BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,592,725	T→C	L385L (TTA→TTG)	ypfI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,592,725	0	T	C	82.6%	594.6 / 119.8	276	L385L (TTA→TTG)	ypfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (29/19); new base C (110/118); total (139/137)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 282 total aligned reads displayed.

CCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCTGTTC  >  NC_000913/2592633‑2592817

  aCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTTGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCaa                                                                                            >  1:2008190/1‑93 (MQ=255)

             ccaGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAgcggcg                                                                                 >  1:93781/1‑93 (MQ=255)

             ccaGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCCGGTGCATCCATCATCCGGCGCAAATCCAgcggcg                                                                                 >  2:5334071/1‑93 (MQ=255)

              caGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGa                                                                                >  1:5979438/1‑93 (MQ=255)

               aGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGaa                                                                               <  2:3244265/93‑1 (MQ=255)

                gCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAg                                                                              >  1:5559995/1‑93 (MQ=255)

                 ccACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGACGTAAATCCAGCGGCGAAGt                                                                             >  2:2314122/1‑93 (MQ=255)

                  cacaGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTc                                                                            >  1:1704138/1‑93 (MQ=255)

                  cacaGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTc                                                                            <  1:3992712/93‑1 (MQ=255)

                  cacaGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTc                                                                            >  2:478626/1‑93 (MQ=255)

                   acaGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTcc                                                                           >  1:2501872/1‑93 (MQ=255)

                   acaGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTcc                                                                           >  2:1867322/1‑93 (MQ=255)

                    caGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCg                                                                          >  1:2601567/1‑93 (MQ=255)

                    caGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCg                                                                          >  2:1045087/1‑93 (MQ=255)

                     aGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTACGa                                                                         <  1:1147965/93‑1 (MQ=255)

                       cgcCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATa                                                                       <  1:6863884/93‑1 (MQ=255)

                        gcCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAg                                                                      <  2:912009/93‑1 (MQ=255)

                         ccccGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGt                                                                     >  1:3581378/1‑93 (MQ=255)

                          cccGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTg                                                                    <  2:5927057/93‑1 (MQ=255)

                           ccGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTgc                                                                   <  1:1362923/93‑1 (MQ=255)

                            cGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTgcg                                                                  >  1:2736342/1‑93 (MQ=255)

                             ggCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTgcgc                                                                 >  1:4403884/1‑93 (MQ=255)

                              gCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCa                                                                <  2:3472167/93‑1 (MQ=255)

                              gCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCa                                                                <  2:5260345/93‑1 (MQ=255)

                               cAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCAc                                                               <  2:5757944/93‑1 (MQ=255)

                                aaTCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTCGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCAcc                                                              >  2:2109730/1‑93 (MQ=255)

                                 aTCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCa                                                             <  1:4675725/93‑1 (MQ=255)

                                  tctcGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAg                                                            <  2:4380950/93‑1 (MQ=255)

                                   ctcGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGa                                                           <  1:838139/93‑1 (MQ=255)

                                    tcGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGAc                                                          <  1:2863945/93‑1 (MQ=255)

                                      tttttCGCCTGCCGCCTGTAAAAAATGTTGCCCTGGTGCAACCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGAGCGCACCAGACaa                                                        <  1:1022500/92‑1 (MQ=255)

                                      gTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACaa                                                        >  1:5649199/1‑93 (MQ=255)

                                      gTTTGCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACaa                                                        <  2:6620327/93‑1 (MQ=255)

                                       ttttCGCCAGCCGCCTGTAAAAAATGTTGTCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAag                                                       >  2:2817284/1‑93 (MQ=255)

                                       ttttCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAag                                                       >  1:374161/1‑93 (MQ=255)

                                        tttCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAaga                                                      <  1:4850884/93‑1 (MQ=255)

                                        tttCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAaga                                                      <  2:2794905/93‑1 (MQ=255)

                                          tCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGc                                                    <  2:4148156/93‑1 (MQ=255)

                                            gCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTg                                                  <  1:2354118/93‑1 (MQ=255)

                                               aGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATa                                               <  1:1000984/93‑1 (MQ=255)

                                               aGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATa                                               >  2:4244085/1‑93 (MQ=255)

                                               aGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATa                                               <  1:6202248/93‑1 (MQ=255)

                                                 ccgccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATaaa                                             <  1:5196320/93‑1 (MQ=255)

                                                  cgccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAAc                                            <  1:4256442/93‑1 (MQ=255)

                                                   gccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAAcc                                           >  2:502798/1‑93 (MQ=255)

                                                   gccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAAcc                                           >  2:2018499/1‑93 (MQ=255)

                                                   gccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAAcc                                           >  1:3054904/1‑93 (MQ=255)

                                                    ccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCt                                          <  1:3421844/93‑1 (MQ=255)

                                                    ccTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCt                                          <  1:4459117/93‑1 (MQ=255)

                                                      tGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCttt                                        >  2:6990425/1‑93 (MQ=255)

                                                      tGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCttt                                        <  1:4485028/93‑1 (MQ=255)

                                                      gtaaaaaaaTTTTGCCCTGGTGCATCCACCATCGGGCGCAAATCCAGGGCCGAAGTCCGATAGTGCGCA‑CAGACAAGAGCTGATAAACCTTTa                                       <  2:6100788/90‑1 (MQ=255)

                                                       gTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTa                                       <  2:5632814/93‑1 (MQ=255)

                                                        tAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTaa                                      >  2:3067648/1‑93 (MQ=255)

                                                         aaaaaaTGTTGCCCTGTTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAAc                                     <  2:4664191/93‑1 (MQ=255)

                                                         aaaaaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGTGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAAc                                     <  2:6672349/93‑1 (MQ=255)

                                                          aaaaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACg                                    <  2:7057563/93‑1 (MQ=255)

                                                          aaaaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACg                                    <  1:5854165/93‑1 (MQ=255)

                                                           aaaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACgg                                   <  2:4533534/93‑1 (MQ=255)

                                                            aaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGc                                  >  2:2647648/1‑93 (MQ=255)

                                                            aaaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGc                                  <  1:5250095/93‑1 (MQ=255)

                                                             aaTGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCg                                 >  2:1593473/1‑93 (MQ=255)

                                                               tGTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGtt                               <  2:3743269/93‑1 (MQ=255)

                                                                gTTGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGttt                              >  2:2960826/1‑93 (MQ=255)

                                                                 ttGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTc                             <  1:3921221/93‑1 (MQ=255)

                                                                 ttGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTc                             <  2:3299784/93‑1 (MQ=255)

                                                                  tGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCt                            <  1:6094615/93‑1 (MQ=255)

                                                                  tGCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCt                            <  1:4132813/93‑1 (MQ=255)

                                                                   gCCCTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTg                           >  2:1348321/1‑93 (MQ=255)

                                                                     ccTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGa                         >  2:2009468/1‑93 (MQ=255)

                                                                     ccTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGa                         <  1:6808642/93‑1 (MQ=255)

                                                                     ccTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGa                         <  1:1951473/93‑1 (MQ=255)

                                                                     ccTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGa                         >  2:872032/1‑93 (MQ=255)

                                                                      cTGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGAt                        >  2:3856325/1‑93 (MQ=255)

                                                                       tGGTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATc                       <  1:7351983/93‑1 (MQ=255)

                                                                        ggTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCg                      >  1:6475793/1‑93 (MQ=255)

                                                                         gTGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGc                     >  1:3659874/1‑93 (MQ=255)

                                                                          tGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCt                    <  1:6644071/93‑1 (MQ=255)

                                                                          tGCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCt                    <  1:631559/93‑1 (MQ=255)

                                                                           gCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCtt                   <  2:7362232/93‑1 (MQ=255)

                                                                           gCATCCATCATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCtt                   >  2:6433828/1‑93 (MQ=255)

                                                                            catccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCttt                  <  2:6234463/93‑1 (MQ=255)

                                                                            catccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCttt                  <  2:6335801/93‑1 (MQ=255)

                                                                             atccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTg                 >  2:2587696/1‑93 (MQ=255)

                                                                             atccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTg                 >  1:2949754/1‑93 (MQ=255)

                                                                              tccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGc                >  1:2218976/1‑93 (MQ=255)

                                                                              tccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGc                >  2:6849236/1‑93 (MQ=255)

                                                                              tccatcATCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGc                >  1:1428943/1‑93 (MQ=255)

                                                                                catcatCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCa              >  1:1236422/1‑93 (MQ=255)

                                                                                catcatCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCa              >  1:5406190/1‑93 (MQ=255)

                                                                                catcatCCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCa              >  2:1280158/1‑93 (MQ=255)

                                                                                   catcCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATaa           <  1:4985263/93‑1 (MQ=255)

                                                                                   catcCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATaa           <  2:1924449/93‑1 (MQ=255)

                                                                                    atcCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAAc          >  2:5435392/1‑93 (MQ=255)

                                                                                    atcCGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAAc          <  2:3969320/93‑1 (MQ=255)

                                                                                      ccGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGt        <  2:7498674/93‑1 (MQ=255)

                                                                                       cGGCGCAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTc       <  2:4305494/93‑1 (MQ=255)

                                                                                        ggCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGGGGTTCTGGATCGCTTTGCCATAACGTCt      >  2:5218016/1‑93 (MQ=255)

                                                                                        ggCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCt      >  2:245886/1‑93 (MQ=255)

                                                                                         gcgcAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCTg     <  1:1584105/93‑1 (MQ=255)

CCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCTGTTC  >  NC_000913/2592633‑2592817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`