BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,610,461	T→C	I181T (ATC→ACC)	b2491 →	HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,610,461	0	T	C	86.8%	709.9 / 93.1	287	I181T (ATC→ACC)	b2491	HyfR
Reads supporting (aligned to +/- strand): ref base T (26/12); new base C (126/123); total (152/135)
Fisher's exact test for biased strand distribution p-value = 5.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 293 total aligned reads displayed.

TCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCTTTCTG  >  NC_000913/2610369‑2610553

  gcgATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGAccc                                                                                            >  2:6459264/1‑93 (MQ=255)

  gcgATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGAccc                                                                                            <  1:2482915/93‑1 (MQ=255)

     atcatcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCcatc                                                                                         >  2:4390348/1‑93 (MQ=255)

     atcatcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCcatc                                                                                         >  1:3077182/1‑93 (MQ=255)

      tcatcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCcatca                                                                                        >  1:5629206/1‑93 (MQ=255)

      tcatcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCcatca                                                                                        >  2:2771964/1‑93 (MQ=255)

       catcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCcatcat                                                                                       <  2:253329/93‑1 (MQ=255)

         tcatcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCAttt                                                                                     <  1:2205330/93‑1 (MQ=255)

          catcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCAtttt                                                                                    <  2:5538163/93‑1 (MQ=255)

           atcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCAttttt                                                                                   >  2:2189393/1‑93 (MQ=255)

           atcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCAttttt                                                                                   >  2:146556/1‑93 (MQ=255)

            tcGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCAtttttt                                                                                  <  1:3227964/93‑1 (MQ=255)

                gTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGt                                                                              >  2:694326/1‑93 (MQ=255)

                gTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGt                                                                              <  2:7203844/93‑1 (MQ=255)

                 tACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTc                                                                             >  2:3144060/1‑93 (MQ=255)

                 tACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTc                                                                             >  1:267260/1‑93 (MQ=255)

                 tACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTc                                                                             <  1:6757203/93‑1 (MQ=255)

                    tGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTgg                                                                          >  2:7507648/1‑93 (MQ=255)

                    tGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTgg                                                                          >  1:5802419/1‑93 (MQ=255)

                      gTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCt                                                                        >  1:857375/1‑93 (MQ=255)

                      gTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCt                                                                        >  1:4215832/1‑93 (MQ=255)

                       ttGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCtt                                                                       >  1:3836837/1‑93 (MQ=255)

                         gATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtca                                                                     >  1:778488/1‑93 (MQ=255)

                         gATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtca                                                                     <  2:1789992/93‑1 (MQ=255)

                          atatTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcag                                                                    >  2:1588353/1‑93 (MQ=255)

                           tatTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcagt                                                                   >  1:6827099/1‑93 (MQ=255)

                           tatTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcagt                                                                   >  1:5351960/1‑93 (MQ=255)

                            atTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcagtc                                                                  <  1:2422542/93‑1 (MQ=255)

                             ttACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca                                                                 >  2:5871176/1‑93 (MQ=255)

                             ttACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca                                                                 >  2:3562192/1‑93 (MQ=255)

                             ttACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcagtca                                                                 >  1:2893180/1‑93 (MQ=255)

                             ttACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTtcagtca                                                                 <  1:4283850/93‑1 (MQ=255)

                                cTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa                                                              >  1:1292593/1‑93 (MQ=255)

                                cTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa                                                              <  2:1739973/93‑1 (MQ=255)

                                cTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa                                                              <  2:3671857/93‑1 (MQ=255)

                                 tAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTAt                                                             >  2:3288489/1‑93 (MQ=255)

                                   aTGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg                                                           <  1:5994067/93‑1 (MQ=255)

                                      cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc                                                        <  1:3391822/93‑1 (MQ=255)

                                      cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc                                                        <  2:877430/93‑1 (MQ=255)

                                         tGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTgg                                                     >  2:4322354/1‑93 (MQ=255)

                                          gCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTggg                                                    >  1:6365052/1‑93 (MQ=255)

                                            tGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCg                                                  >  1:4511281/1‑93 (MQ=255)

                                             gTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa                                                 >  2:101965/1‑93 (MQ=255)

                                              tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat                                                >  2:6304763/1‑93 (MQ=255)

                                              tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat                                                <  2:5657411/93‑1 (MQ=255)

                                              tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat                                                >  2:4896042/1‑93 (MQ=255)

                                              tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat                                                <  1:6920256/93‑1 (MQ=255)

                                                acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatca                                              >  1:7184767/1‑93 (MQ=255)

                                                acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatca                                              <  1:1756350/93‑1 (MQ=255)

                                                 caTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatcat                                             >  1:2689396/1‑93 (MQ=255)

                                                  aTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatcatc                                            <  1:6271720/93‑1 (MQ=255)

                                                  aTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatcatc                                            <  2:6071795/93‑1 (MQ=255)

                                                   tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCg                                           >  2:3762726/1‑93 (MQ=255)

                                                   tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCg                                           >  2:697409/1‑93 (MQ=255)

                                                   tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCg                                           >  1:1964583/1‑93 (MQ=255)

                                                     ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGaa                                         >  1:6609598/1‑93 (MQ=255)

                                                     ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGaa                                         >  2:4647647/1‑93 (MQ=255)

                                                      tGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGaaa                                        <  1:733223/93‑1 (MQ=255)

                                                        aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGa                                      >  1:939420/1‑93 (MQ=255)

                                                        aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGa                                      <  1:6574594/93‑1 (MQ=255)

                                                          ctcGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAAc                                    <  2:735337/93‑1 (MQ=255)

                                                          ctcGACGATCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGCATGGTACTGGGCGATCATCGAAAGAAc                                    <  1:2778776/93‑1 (MQ=255)

                                                            cgacgaTCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACga                                  <  2:4119520/93‑1 (MQ=255)

                                                              acgaTCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTGGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgaga                                >  1:6073322/1‑93 (MQ=255)

                                                              acgaTCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgaga                                >  1:4723078/1‑93 (MQ=255)

                                                                gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAg                              >  2:6088634/1‑93 (MQ=255)

                                                                 aTCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt                             >  1:1702972/1‑93 (MQ=255)

                                                                 aTCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt                             <  1:648002/93‑1 (MQ=255)

                                                                  tCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGtt                            >  2:2405726/1‑93 (MQ=255)

                                                                  tCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGtt                            <  2:389270/93‑1 (MQ=255)

                                                                  tCTGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGtt                            >  2:715016/1‑93 (MQ=255)

                                                                    tGATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCa                          <  2:4560379/93‑1 (MQ=255)

                                                                     gATCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAg                         >  1:4462904/1‑93 (MQ=255)

                                                                      aTCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGc                        <  2:4808759/93‑1 (MQ=255)

                                                                       tCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGcc                       <  1:1194985/93‑1 (MQ=255)

                                                                       tCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGcc                       >  2:510532/1‑93 (MQ=255)

                                                                       tCGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGcc                       >  1:5519390/1‑93 (MQ=255)

                                                                        cGCTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCt                      <  2:6007207/93‑1 (MQ=255)

                                                                          cTGACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgt                    <  2:75542/93‑1 (MQ=255)

                                                                            gACGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg                  >  2:6654918/1‑93 (MQ=255)

                                                                             aCGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtggt                 <  1:539405/93‑1 (MQ=255)

                                                                              cGTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtggtg                <  2:6364539/93‑1 (MQ=255)

                                                                               gTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGc               >  2:2009650/1‑93 (MQ=255)

                                                                               gTCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGc               >  2:5242117/1‑93 (MQ=255)

                                                                                tCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCa              >  1:2957940/1‑93 (MQ=255)

                                                                                tCGCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCa              >  1:5073505/1‑93 (MQ=255)

                                                                                 cGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAg             <  2:5949248/93‑1 (MQ=255)

                                                                                  gCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGc            <  1:3682501/93‑1 (MQ=255)

                                                                                  gCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGc            <  2:1535127/93‑1 (MQ=255)

                                                                                  gCTCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGc            <  2:1109026/93‑1 (MQ=255)

                                                                                    tCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGa          <  1:1029212/93‑1 (MQ=255)

                                                                                    tCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGa          >  2:3831/1‑93 (MQ=255)

                                                                                    tCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGa          <  1:5203277/93‑1 (MQ=255)

                                                                                    tCGTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGa          >  1:5495137/1‑93 (MQ=255)

                                                                                      gTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATc        >  2:2649958/1‑93 (MQ=255)

                                                                                      gTGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATc        <  1:492303/93‑1 (MQ=255)

                                                                                       tGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCt       <  1:5718984/93‑1 (MQ=255)

                                                                                       tGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCt       <  2:2800121/93‑1 (MQ=255)

                                                                                       tGAGACCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCt       <  1:1592593/93‑1 (MQ=255)

                                                                                         agaCCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCttt     <  1:2806932/93‑1 (MQ=255)

TCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCTTTCTG  >  NC_000913/2610369‑2610553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`