BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,616,953	T→C	S410G (AGT→GGT)	uraA ←	UraA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,616,953	0	T	C	84.1%	636.2 / 112.4	283	S410G (AGT→GGT)	uraA	UraA
Reads supporting (aligned to +/- strand): ref base T (28/17); new base C (118/120); total (146/137)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-02

Only 100 of 284 total aligned reads displayed.

ACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCG  >  NC_000913/2616861‑2617044

aCCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACt                                                                                             >  2:6190209/1‑93 (MQ=255)

    ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATc                                                                                         <  2:190108/93‑1 (MQ=255)

     gcagcagcaCTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCa                                                                                        <  1:1359803/93‑1 (MQ=255)

      cagcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAg                                                                                       >  2:5558771/1‑93 (MQ=255)

       agcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGt                                                                                      >  2:5707561/1‑93 (MQ=255)

       agcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGt                                                                                      >  1:5353269/1‑93 (MQ=255)

        gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:6327659/1‑93 (MQ=255)

        gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:3949598/1‑93 (MQ=255)

        gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:3884437/1‑93 (MQ=255)

         caACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGttt                                                                                    <  1:3454938/93‑1 (MQ=255)

          aaCACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTg                                                                                   >  1:2498112/1‑93 (MQ=255)

           acacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGa                                                                                  >  1:833049/1‑93 (MQ=255)

            cacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGaa                                                                                 <  2:365923/93‑1 (MQ=255)

            cacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGaa                                                                                 <  1:4869469/93‑1 (MQ=255)

              cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGa                                                                               <  1:4595118/93‑1 (MQ=255)

              cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGa                                                                               <  2:2746611/93‑1 (MQ=255)

              cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGa                                                                               <  1:4118072/93‑1 (MQ=255)

              cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGa                                                                               <  1:3299638/93‑1 (MQ=255)

               tGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGAt                                                                              >  2:2762822/1‑93 (MQ=255)

                gCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATc                                                                             >  1:1112608/1‑93 (MQ=255)

                gCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATc                                                                             >  2:2509141/1‑93 (MQ=255)

                  ccGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaa                                                                           >  2:1293366/1‑93 (MQ=255)

                  ccGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaa                                                                           <  2:3787182/93‑1 (MQ=255)

                  ccGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaa                                                                           <  2:3883161/93‑1 (MQ=255)

                   cggtcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaaa                                                                          >  1:5003133/1‑93 (MQ=255)

                       cggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTa                                                                      >  2:1475046/1‑93 (MQ=255)

                        ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCCCTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     <  1:6911489/93‑1 (MQ=255)

                        ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     >  2:4781519/1‑93 (MQ=255)

                        ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     <  2:6066409/93‑1 (MQ=255)

                         gtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAg                                                                    <  1:983896/93‑1 (MQ=255)

                         gtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAg                                                                    >  1:1667100/1‑93 (MQ=255)

                         gtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAg                                                                    >  2:3312213/1‑93 (MQ=255)

                           aCATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGAACAGTTTGAAGATCAAACTTAAGcc                                                                  >  2:716923/1‑93 (MQ=255)

                            cATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCg                                                                 >  1:4617583/1‑93 (MQ=255)

                             attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                <  2:2104862/93‑1 (MQ=255)

                             attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                >  2:4750254/1‑93 (MQ=255)

                             attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                >  1:2656227/1‑93 (MQ=255)

                             attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                >  2:5097214/1‑93 (MQ=255)

                              ttattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGAt                                                               <  1:2753190/93‑1 (MQ=255)

                               tattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATa                                                              <  1:435077/93‑1 (MQ=255)

                               tattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATa                                                              <  1:2017341/93‑1 (MQ=255)

                                 tttGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATAcc                                                            >  2:1220583/1‑93 (MQ=255)

                                    gTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgac                                                         <  1:177795/93‑1 (MQ=255)

                                     tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacg                                                        <  2:1457892/93‑1 (MQ=255)

                                      cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                       <  2:4681332/93‑1 (MQ=255)

                                       tGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGAt                                                      <  2:4245658/93‑1 (MQ=255)

                                          tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGt                                                   <  2:2868323/93‑1 (MQ=255)

                                          tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGt                                                   <  1:6507774/93‑1 (MQ=255)

                                           atatCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTc                                                  <  2:6320961/93‑1 (MQ=255)

                                            tatCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCg                                                 >  1:6830372/1‑93 (MQ=255)

                                            tatCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCg                                                 >  1:7460767/1‑93 (MQ=255)

                                             atCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGc                                                >  1:3080639/1‑93 (MQ=255)

                                             atCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGc                                                >  2:5005844/1‑93 (MQ=255)

                                             atCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGc                                                >  1:5498205/1‑93 (MQ=255)

                                              tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGcc                                               >  1:6637574/1‑93 (MQ=255)

                                              tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGcc                                               >  2:6920165/1‑93 (MQ=255)

                                                cgcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAg                                             >  1:6716714/1‑93 (MQ=255)

                                                cgcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAg                                             <  2:5232205/93‑1 (MQ=255)

                                                 gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            >  2:5440884/1‑93 (MQ=255)

                                                 gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            >  2:1609859/1‑93 (MQ=255)

                                                 gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            >  1:5156029/1‑93 (MQ=255)

                                                 gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            >  1:4430623/1‑93 (MQ=255)

                                                  cgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcg                                           <  2:5993544/93‑1 (MQ=255)

                                                    tcttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGcc                                         >  1:2926401/1‑93 (MQ=255)

                                                     cttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCa                                        >  2:4811149/1‑93 (MQ=255)

                                                     cttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCa                                        <  2:2385119/93‑1 (MQ=255)

                                                      ttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCAt                                       >  2:4383335/1‑93 (MQ=255)

                                                      ttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCAt                                       >  1:6034275/1‑93 (MQ=255)

                                                          gCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCt                                   <  2:1109463/93‑1 (MQ=255)

                                                            gTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCttt                                 <  2:1753341/93‑1 (MQ=255)

                                                            gTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCttt                                 >  2:5255226/1‑93 (MQ=255)

                                                            gTCCAGCACCACTTCGTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCttt                                 <  1:1394938/93‑1 (MQ=255)

                                                             tCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCtttt                                >  2:2635467/1‑93 (MQ=255)

                                                              ccAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTa                               >  1:3604216/1‑93 (MQ=255)

                                                                aGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAAc                             >  1:5398938/1‑93 (MQ=255)

                                                                 gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            >  1:1170771/1‑93 (MQ=255)

                                                                 gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            >  1:1638707/1‑93 (MQ=255)

                                                                  caccacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTc                           >  1:616521/1‑93 (MQ=255)

                                                                  caccacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTc                           <  1:5717693/93‑1 (MQ=255)

                                                                     cacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCGAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccgc                        <  2:4008615/93‑1 (MQ=255)

                                                                     cacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccgc                        <  2:3122402/93‑1 (MQ=255)

                                                                        ttcttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgc                     >  1:6109373/1‑93 (MQ=255)

                                                                        ttcttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgc                     >  1:5071205/1‑93 (MQ=255)

                                                                         tcttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcg                    >  2:7526332/1‑93 (MQ=255)

                                                                          cttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgc                   <  2:1080163/93‑1 (MQ=255)

                                                                          cttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgc                   <  1:1298671/93‑1 (MQ=255)

                                                                           ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgcc                  >  1:2622809/1‑93 (MQ=255)

                                                                           ttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgcc                  <  2:362776/93‑1 (MQ=255)

                                                                           ttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgcc                  <  2:7338396/93‑1 (MQ=255)

                                                                             cAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGa                <  2:6672077/93‑1 (MQ=255)

                                                                               gggNGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATg              <  1:5808291/93‑1 (MQ=255)

                                                                               gggCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATg              >  2:5863589/1‑93 (MQ=255)

                                                                               gggCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATg              <  2:5811388/93‑1 (MQ=255)

                                                                                  cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGttt           <  2:5356635/93‑1 (MQ=255)

                                                                                  cgcAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGttt           <  1:7083505/93‑1 (MQ=255)

                                                                                  cgcAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGttt           <  1:1083436/93‑1 (MQ=255)

                                                                                   gcagcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTa          >  2:4559666/1‑93 (MQ=255)

                                                                                      gcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCt       <  1:218612/93‑1 (MQ=255)

                                                                                      gcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCt       >  2:6707768/1‑93 (MQ=255)

                                                                                        acacCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTc     >  2:7245654/1‑93 (MQ=255)

ACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCG  >  NC_000913/2616861‑2617044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`