BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,647,757	G→A	P851S (CCG→TCG)	b2520 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,647,757	0	G	A	89.5%	841.3 / 51.6	276	P851S (CCG→TCG)	b2520	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (10/19); new base A (123/124); total (133/143)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 277 total aligned reads displayed.

CTTTAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGA  >  NC_000913/2647667‑2647848

cTTTAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACa                                                                                           <  2:6560559/93‑1 (MQ=255)

  ttAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGa                                                                                         <  1:3592893/93‑1 (MQ=255)

  ttAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGa                                                                                         <  1:6464412/93‑1 (MQ=255)

  ttAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGa                                                                                         <  1:6205710/93‑1 (MQ=255)

  ttAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGa                                                                                         <  1:5206746/93‑1 (MQ=255)

   ttaTTTTTCCGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGAt                                                                                        <  1:1479408/91‑1 (MQ=255)

    aaTTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATg                                                                                       <  1:5097865/93‑1 (MQ=255)

     aTTTTCACGGTTAATGGTTGATTGGGATGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGc                                                                                      <  2:3440936/93‑1 (MQ=255)

      ttttCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCa                                                                                     >  2:3875273/1‑93 (MQ=255)

       tttCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCaa                                                                                    <  2:6715504/93‑1 (MQ=255)

       tttCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCaa                                                                                    <  1:2731362/93‑1 (MQ=255)

        ttCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAac                                                                                   <  1:5719866/93‑1 (MQ=255)

         tCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAaca                                                                                  <  1:2405265/93‑1 (MQ=255)

         tCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAaca                                                                                  >  2:251834/1‑93 (MQ=255)

           aCGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAacaca                                                                                >  1:1223834/1‑93 (MQ=255)

           aCGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAacaca                                                                                >  1:794679/1‑93 (MQ=255)

            cGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAacacac                                                                               <  1:4700092/93‑1 (MQ=255)

             ggTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACAcc                                                                              >  2:3794981/1‑93 (MQ=255)

              gTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCa                                                                             >  1:345835/1‑93 (MQ=255)

              gTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCa                                                                             >  1:3776059/1‑93 (MQ=255)

               ttAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCaa                                                                            <  2:240672/93‑1 (MQ=255)

                tAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAAc                                                                           <  2:3091453/93‑1 (MQ=255)

                  aTGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACcg                                                                         >  1:2923706/1‑93 (MQ=255)

                  aTGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACcg                                                                         >  2:214881/1‑93 (MQ=255)

                   tGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACcgc                                                                        >  1:6608731/1‑93 (MQ=255)

                          ttGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGtttt                                                                 >  1:3918721/1‑93 (MQ=255)

                          ttGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGtttt                                                                 >  1:4737371/1‑93 (MQ=255)

                          ttGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGtttt                                                                 >  2:5293541/1‑93 (MQ=255)

                          ttGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGtttt                                                                 >  1:4909125/1‑93 (MQ=255)

                          ttGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGtttt                                                                 <  2:1890824/93‑1 (MQ=255)

                               aCGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGt                                                            <  2:7301771/93‑1 (MQ=255)

                                cGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTc                                                           >  1:3742149/1‑93 (MQ=255)

                                cGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTc                                                           >  1:6095571/1‑93 (MQ=255)

                                  cATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTcgc                                                         >  1:1964558/1‑93 (MQ=255)

                                   aTTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTcgcg                                                        >  2:6495697/1‑93 (MQ=255)

                                   aTTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTcgcg                                                        <  2:4789004/93‑1 (MQ=255)

                                   aTTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTcgcg                                                        <  2:4336303/93‑1 (MQ=255)

                                    tttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCgg                                                       >  2:5551031/1‑93 (MQ=255)

                                    tttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCgg                                                       >  2:1097314/1‑93 (MQ=255)

                                    tttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCgg                                                       >  1:4329968/1‑93 (MQ=255)

                                    tttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCgg                                                       >  1:4012362/1‑93 (MQ=255)

                                     ttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGa                                                      <  1:4436855/93‑1 (MQ=255)

                                     ttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGa                                                      <  2:6934069/93‑1 (MQ=255)

                                     ttttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGa                                                      <  2:1693260/93‑1 (MQ=255)

                                      tttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAg                                                     >  2:6139401/1‑93 (MQ=255)

                                       ttGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGc                                                    >  2:7169657/1‑93 (MQ=255)

                                         gCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCga                                                  <  2:1254945/93‑1 (MQ=255)

                                           tGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCgaga                                                <  2:4867297/93‑1 (MQ=255)

                                            ggTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGAt                                               >  2:6341342/1‑93 (MQ=255)

                                            ggTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGAt                                               >  2:6002586/1‑93 (MQ=255)

                                                 ttCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATc                                          <  2:892434/93‑1 (MQ=255)

                                                  tCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCg                                         >  1:3376820/1‑93 (MQ=255)

                                                    cAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGcc                                       <  2:7373047/93‑1 (MQ=255)

                                                     aGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCa                                      >  2:4920698/1‑93 (MQ=255)

                                                     aGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCa                                      <  2:1993641/93‑1 (MQ=255)

                                                     aGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCa                                      >  1:5341254/1‑93 (MQ=255)

                                                      gcgcCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAg                                     <  1:863519/93‑1 (MQ=255)

                                                      gcgcCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAg                                     <  2:4012712/93‑1 (MQ=255)

                                                       cgcCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAgg                                    <  1:231086/93‑1 (MQ=255)

                                                        gcCAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGa                                   >  2:7044950/1‑93 (MQ=255)

                                                         ccAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGAc                                  <  2:6374344/93‑1 (MQ=255)

                                                         acAGATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGAc                                  <  2:4649949/92‑1 (MQ=255)

                                                            gATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTa                               >  1:6580494/1‑93 (MQ=255)

                                                            gATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTa                               >  2:1465773/1‑93 (MQ=255)

                                                            gATCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTa                               <  1:3206235/93‑1 (MQ=255)

                                                             aTCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTac                              <  2:2983441/93‑1 (MQ=255)

                                                             aTCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTac                              >  1:4553868/1‑93 (MQ=255)

                                                             aTCGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTac                              >  1:255777/1‑93 (MQ=255)

                                                               cGAGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTacca                            <  1:7399964/93‑1 (MQ=255)

                                                                 aGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTaccacc                          <  2:371715/93‑1 (MQ=255)

                                                                 aGGCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTaccacc                          >  2:4384110/1‑93 (MQ=255)

                                                                 aGGCGACGGCTTTCATCGCCAAGCGACAGCTGCCACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGTTTTATCGCCAGGACGTaccacc                          >  1:537925/1‑93 (MQ=255)

                                                                  ggCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTAccacca                         <  1:3055308/93‑1 (MQ=255)

                                                                  ggCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTAccacca                         >  2:5870560/1‑93 (MQ=255)

                                                                   gCGACGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAg                        <  2:3157448/93‑1 (MQ=255)

                                                                      aCGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGt                     >  1:6377128/1‑93 (MQ=255)

                                                                      aCGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGt                     >  1:6769033/1‑93 (MQ=255)

                                                                      aCGGTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGt                     >  2:6663139/1‑93 (MQ=255)

                                                                        ggTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTAc                   <  2:234451/93‑1 (MQ=255)

                                                                         gTTTTCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACt                  >  2:1369824/1‑93 (MQ=255)

                                                                          ttttCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACtt                 >  1:7303963/1‑93 (MQ=255)

                                                                             tCATCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTaaa              <  2:6239960/93‑1 (MQ=255)

                                                                               aTCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATa            <  1:1563394/93‑1 (MQ=255)

                                                                               aTCGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATa            >  1:1011283/1‑93 (MQ=255)

                                                                                 cGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACa          <  2:2052945/93‑1 (MQ=255)

                                                                                 cGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACa          <  2:2497933/93‑1 (MQ=255)

                                                                                 cGCCAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACa          <  1:7094604/93‑1 (MQ=255)

                                                                                  gCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAg         >  2:2783553/1‑93 (MQ=255)

                                                                                   ccAAGGGGCAAGTTCAACCCCCCCACCGCGGGTTTTGGGGGTGCGGAGCGGGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGa        <  1:6385273/93‑1 (MQ=255)

                                                                                   ccAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGa        <  1:4874924/93‑1 (MQ=255)

                                                                                   ccAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGa        <  1:1899057/93‑1 (MQ=255)

                                                                                   ccAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGa        <  2:4288536/93‑1 (MQ=255)

                                                                                    cAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGAt       >  1:6825237/1‑93 (MQ=255)

                                                                                    cAAGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGAt       >  2:3749960/1‑93 (MQ=255)

                                                                                     aaGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATc      <  1:5836279/93‑1 (MQ=255)

                                                                                      aGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCa     >  1:2223286/1‑93 (MQ=255)

                                                                                      aGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCa     <  1:6873071/93‑1 (MQ=255)

                                                                                      aGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCa     <  1:5520144/93‑1 (MQ=255)

                                                                                      aGCGACAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCa     >  2:422150/1‑93 (MQ=255)

                                                                                         ggCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGa  <  2:7198442/93‑1 (MQ=255)

CTTTAATTTTCACGGTTAATGGTTGATTGGGACGCATTTTTGCTGGTGTTTCCAGCGCCAGATCGAGGCGACGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGA  >  NC_000913/2647667‑2647848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`