BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,748	A→G	V135A (GTG→GCG)	b2531 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,748	0	A	G	85.2%	618.0 / 99.5	270	V135A (GTG→GCG)	b2531	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (25/15); new base G (116/114); total (141/129)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 273 total aligned reads displayed.

CTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCA  >  NC_000913/2659656‑2659838

cTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCa                                                                                            >  1:6730507/1‑93 (MQ=255)

cTTTTTATTAAGCGAGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCa                                                                                            >  2:4767126/1‑93 (MQ=255)

  ttttATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCt                                                                                          >  1:5578666/1‑93 (MQ=255)

   tttATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACtt                                                                                         >  1:5015937/1‑93 (MQ=255)

   tttATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACtt                                                                                         <  1:6452749/93‑1 (MQ=255)

    ttattaAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTc                                                                                        <  1:1290372/93‑1 (MQ=255)

       ttaAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTg                                                                                     >  2:5922012/1‑93 (MQ=255)

       ttaAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTg                                                                                     >  2:683534/1‑93 (MQ=255)

       ttaAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTg                                                                                     <  2:3710253/93‑1 (MQ=255)

        taAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTgg                                                                                    <  2:6189459/93‑1 (MQ=255)

        taAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTgg                                                                                    <  2:5259924/93‑1 (MQ=255)

        taAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTgg                                                                                    <  2:2352677/93‑1 (MQ=255)

        taAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTgg                                                                                    <  1:1452943/93‑1 (MQ=255)

           gcgcGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGtta                                                                                 <  2:2214546/93‑1 (MQ=255)

              cgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGttatta                                                                              >  1:5861381/1‑93 (MQ=255)

               gTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTaa                                                                             >  2:6079847/1‑93 (MQ=255)

                tAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAAc                                                                            <  1:1781114/93‑1 (MQ=255)

                tAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAAc                                                                            <  1:3922233/93‑1 (MQ=255)

                 aaCTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAAcc                                                                           >  2:7211173/1‑93 (MQ=255)

                 aaCTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAAcc                                                                           <  1:7331155/93‑1 (MQ=255)

                 aaCTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAAcc                                                                           >  1:940939/1‑93 (MQ=255)

                     tAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGtt                                                                       <  2:5779924/93‑1 (MQ=255)

                     tAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGtt                                                                       <  2:4080474/93‑1 (MQ=255)

                       aCGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGGCGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCg                                                                     >  1:7372587/1‑93 (MQ=255)

                        cGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGc                                                                    >  2:2858066/1‑93 (MQ=255)

                        cGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGc                                                                    <  1:4543987/93‑1 (MQ=255)

                         gTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGcc                                                                   >  2:2399002/1‑93 (MQ=255)

                         gTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGcc                                                                   >  2:5406583/1‑93 (MQ=255)

                          tCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCt                                                                  >  1:6973595/1‑93 (MQ=255)

                            gATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaa                                                                >  1:6098686/1‑93 (MQ=255)

                            gATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTaa                                                                >  2:660488/1‑93 (MQ=255)

                             aTCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaaa                                                               <  1:678696/93‑1 (MQ=255)

                             aTCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaaa                                                               <  2:172433/93‑1 (MQ=255)

                             aTCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaaa                                                               <  2:319568/93‑1 (MQ=255)

                             aTCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaaa                                                               >  1:789533/1‑93 (MQ=255)

                             aTCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTaaa                                                               >  1:3936938/1‑93 (MQ=255)

                              tCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAg                                                              >  2:4898403/1‑93 (MQ=255)

                               cgcgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGt                                                             <  1:3665781/93‑1 (MQ=255)

                                gcgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTa                                                            >  2:6914891/1‑93 (MQ=255)

                                 cgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTaa                                                           <  2:6372525/93‑1 (MQ=255)

                                  gTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAt                                                          <  1:5157970/93‑1 (MQ=255)

                                   tCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAtg                                                         >  1:1800236/1‑93 (MQ=255)

                                   tCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAtg                                                         >  1:418782/1‑93 (MQ=255)

                                   tCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAtg                                                         >  1:153607/1‑93 (MQ=255)

                                    cTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAtgt                                                        >  2:4639338/1‑93 (MQ=255)

                                      tgtgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAtgttg                                                      <  2:2082566/93‑1 (MQ=255)

                                            cGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTgaga                                                <  1:35945/93‑1 (MQ=255)

                                               gtacGTGGCGATTCGTGAGTATGCTGACGACGGGAAAGATCCTATGCGTCCTGGTTATGAGCCAAGTCGCATAAAGTAATGTTGTTGAGaaaa                                             <  1:1960350/90‑1 (MQ=255)

                                               gTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGaaaa                                             <  1:6906217/93‑1 (MQ=255)

                                                tGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAc                                            <  1:4645532/93‑1 (MQ=255)

                                                tGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAc                                            >  1:2033323/1‑93 (MQ=255)

                                                 gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAcc                                           <  2:2774541/93‑1 (MQ=255)

                                                 gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAcc                                           >  1:2614610/1‑93 (MQ=255)

                                                 gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAcc                                           >  2:6290711/1‑93 (MQ=255)

                                                 gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAAcc                                           <  2:5343629/93‑1 (MQ=255)

                                                  cGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCg                                          <  1:1482704/93‑1 (MQ=255)

                                                    tGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGt                                        >  2:1801570/1‑93 (MQ=255)

                                                      gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTga                                      >  1:3569234/1‑93 (MQ=255)

                                                      gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTga                                      <  1:7064908/93‑1 (MQ=255)

                                                        gcgTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTgaga                                    <  1:2476082/93‑1 (MQ=255)

                                                        gcgTCGTGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTgaga                                    <  1:3392884/93‑1 (MQ=255)

                                                           tcgtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACgg                                 >  1:2974832/1‑93 (MQ=255)

                                                           tcgtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACgg                                 >  2:5843631/1‑93 (MQ=255)

                                                           tcgtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACgg                                 <  2:114067/93‑1 (MQ=255)

                                                           tcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACgg                                 >  1:3858472/1‑93 (MQ=255)

                                                            cgtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGt                                <  1:1225311/93‑1 (MQ=255)

                                                            cgtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGt                                <  2:6556540/93‑1 (MQ=255)

                                                             gtGAGTATGCTGACGACCAGACACATCGAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTc                               >  1:5660274/1‑93 (MQ=255)

                                                             gtGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTc                               >  2:801705/1‑93 (MQ=255)

                                                              tGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCg                              >  2:3585299/1‑93 (MQ=255)

                                                              tGAGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCg                              >  2:1406721/1‑93 (MQ=255)

                                                                aGTATGCTGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCt                            >  2:3163843/1‑93 (MQ=255)

                                                                       tGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCa                     <  2:3435132/93‑1 (MQ=255)

                                                                       tGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCa                     >  1:2082923/1‑93 (MQ=255)

                                                                       tGACGACCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCa                     <  2:3599959/93‑1 (MQ=255)

                                                                        gacgacCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCAc                    <  1:910322/93‑1 (MQ=255)

                                                                        gacgacCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCAc                    >  2:5555483/1‑93 (MQ=255)

                                                                         acgacCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACg                   <  1:4881985/93‑1 (MQ=255)

                                                                           gacCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGcc                 <  2:3999422/93‑1 (MQ=255)

                                                                            acCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCca                >  2:7466791/1‑93 (MQ=255)

                                                                            acCAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCca                >  1:2136722/1‑93 (MQ=255)

                                                                              cAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCcaca              <  2:4283439/93‑1 (MQ=255)

                                                                              cAGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCcaca              >  2:161115/1‑93 (MQ=255)

                                                                               aGACACATCCAGCGCTTCGTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAg             >  2:3921389/1‑93 (MQ=255)

                                                                               aGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAg             <  1:6292687/93‑1 (MQ=255)

                                                                               aGACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAg             >  1:5949804/1‑93 (MQ=255)

                                                                                gACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgc            >  2:159301/1‑93 (MQ=255)

                                                                                gACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgc            <  2:1827497/93‑1 (MQ=255)

                                                                                gACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgc            >  1:6242282/1‑93 (MQ=255)

                                                                                gACACATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgc            <  2:7074887/93‑1 (MQ=255)

                                                                                gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgc            <  1:1352508/93‑1 (MQ=255)

                                                                                 acacATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgcg           >  1:1665881/1‑93 (MQ=255)

                                                                                 acacATCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAgcg           <  1:334398/93‑1 (MQ=255)

                                                                                   acaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGcgcg         >  1:2796194/1‑93 (MQ=255)

                                                                                    caTCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGt        >  1:431203/1‑93 (MQ=255)

                                                                                    caTCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGt        >  1:3184495/1‑93 (MQ=255)

                                                                                      tCCAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTg       >  2:3347004/1‑92 (MQ=255)

                                                                                        cAGCGCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGt    <  1:5378897/93‑1 (MQ=255)

                                                                                        cAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGt    >  1:3453871/1‑93 (MQ=255)

                                                                                        cAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGt    >  2:722002/1‑93 (MQ=255)

CTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCA  >  NC_000913/2659656‑2659838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`