BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,551	T→C	Q178R (CAG→CGG)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,551	0	T	C	88.5%	739.4 / 80.9	286	Q178R (CAG→CGG)	rnc	RNase III
Reads supporting (aligned to +/- strand): ref base T (13/20); new base C (110/143); total (123/163)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

Only 100 of 287 total aligned reads displayed.

CGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTA  >  NC_000913/2701461‑2701643

       aaCCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCa                                                                                     >  1:38608/1‑93 (MQ=255)

       aaCCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCa                                                                                     <  1:3956056/93‑1 (MQ=255)

        gccTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAg                                                                                    >  1:4870840/2‑93 (MQ=255)

         ccTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGa                                                                                   <  2:271931/93‑1 (MQ=255)

           tgtgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATa                                                                                 <  1:675374/93‑1 (MQ=255)

            gtgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATaa                                                                                <  2:5769214/93‑1 (MQ=255)

            gtgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATaa                                                                                <  1:6492168/93‑1 (MQ=255)

            gtgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATaa                                                                                <  1:1071204/93‑1 (MQ=255)

             tgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAg                                                                               >  1:3156682/1‑93 (MQ=255)

             tgCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAg                                                                               <  2:2104139/93‑1 (MQ=255)

                 aaCCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcgg                                                                           >  2:613722/1‑93 (MQ=255)

                  accaccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggc                                                                          >  1:2706145/1‑93 (MQ=255)

                    caccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcag                                                                        >  1:4199002/1‑93 (MQ=255)

                     accGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagc                                                                       >  1:720575/1‑93 (MQ=255)

                     accGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagc                                                                       <  2:3317171/93‑1 (MQ=255)

                     accGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagc                                                                       >  2:5094877/1‑93 (MQ=255)

                     accGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagc                                                                       <  2:748859/93‑1 (MQ=255)

                      ccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcg                                                                      >  1:4110682/1‑93 (MQ=255)

                       cGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcgg                                                                     >  1:4984810/1‑93 (MQ=255)

                       cGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcgg                                                                     <  1:3902999/93‑1 (MQ=255)

                        ggTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggc                                                                    >  1:2254602/1‑93 (MQ=255)

                         gTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggca                                                                   <  2:2438238/93‑1 (MQ=255)

                         gTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggca                                                                   <  2:522573/93‑1 (MQ=255)

                         gTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggca                                                                   >  1:1570764/1‑93 (MQ=255)

                              cTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATgg                                                              >  1:3800695/1‑93 (MQ=255)

                                cAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTAACAGATAAGTCGGCAGCGGCAGATGGCg                                                            <  1:5647485/93‑1 (MQ=255)

                                 aGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGa                                                           <  2:3123157/93‑1 (MQ=255)

                                 aGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGa                                                           <  2:1924770/93‑1 (MQ=255)

                                 aGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGa                                                           <  1:5595033/93‑1 (MQ=255)

                                 aGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGa                                                           <  2:651260/93‑1 (MQ=255)

                                  ggCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAc                                                          <  2:2593099/93‑1 (MQ=255)

                                  ggCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAc                                                          >  2:5611624/1‑93 (MQ=255)

                                  ggCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAc                                                          >  1:5541074/1‑93 (MQ=255)

                                   gCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAcc                                                         <  2:3803975/93‑1 (MQ=255)

                                    ccGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAccc                                                        <  1:6639176/93‑1 (MQ=255)

                                    ccGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAccc                                                        <  2:16470/93‑1 (MQ=255)

                                     cGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCt                                                       >  2:2262197/1‑93 (MQ=255)

                                      gCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTg                                                      >  2:1106651/1‑93 (MQ=255)

                                       cTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGc                                                     <  2:250498/93‑1 (MQ=255)

                                       cTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGc                                                     <  1:4385134/93‑1 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                                  <  2:4072382/93‑1 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                                  <  1:2609524/93‑1 (MQ=255)

                                           ccTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAat                                                 >  2:2768235/1‑93 (MQ=255)

                                            cTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAata                                                >  2:5470186/1‑93 (MQ=255)

                                             tGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAatat                                               <  2:1954231/93‑1 (MQ=255)

                                             tGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAatat                                               >  2:4530440/1‑93 (MQ=255)

                                               gCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTc                                             <  2:4323907/93‑1 (MQ=255)

                                               gCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTc                                             >  2:2517850/1‑93 (MQ=255)

                                                  gTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTg                                          >  2:1530434/1‑93 (MQ=255)

                                                   tGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGc                                         >  1:1089813/1‑93 (MQ=255)

                                                    ggATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCa                                        >  1:6066770/1‑93 (MQ=255)

                                                     gATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCaa                                       >  2:1271860/1‑93 (MQ=255)

                                                      aTAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAg                                      <  2:3614624/93‑1 (MQ=255)

                                                       tAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAgc                                     <  2:5342331/93‑1 (MQ=255)

                                                        aGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAgcg                                    >  1:2029265/1‑93 (MQ=255)

                                                         gTAAATTCCTGATCGTGCGCTTCGCTACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAgcgc                                   <  1:4695108/93‑1 (MQ=255)

                                                          tAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGcgcg                                  >  1:6309713/1‑93 (MQ=255)

                                                           aaaTTCCTGATCGTGCGCTTCGCCGCGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGt                                 <  2:6997918/93‑1 (MQ=255)

                                                           aaaTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGt                                 <  2:1712280/93‑1 (MQ=255)

                                                            aaTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtt                                >  1:1016041/1‑93 (MQ=255)

                                                              ttCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtttt                              >  1:1332310/1‑93 (MQ=255)

                                                              ttCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtttt                              >  2:3957666/1‑93 (MQ=255)

                                                               tCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTc                             <  1:3441721/93‑1 (MQ=255)

                                                               tCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTc                             <  1:2954911/93‑1 (MQ=255)

                                                                 cTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCgg                           >  1:36828/1‑93 (MQ=255)

                                                                 cTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCgg                           <  1:694098/93‑1 (MQ=255)

                                                                  tGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGa                          <  1:1708101/93‑1 (MQ=255)

                                                                  tGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGa                          <  1:865190/93‑1 (MQ=255)

                                                                   gATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGAt                         <  2:6660797/93‑1 (MQ=255)

                                                                   gATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGAt                         <  1:6443009/93‑1 (MQ=255)

                                                                   gATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGAt                         >  1:5144794/1‑93 (MQ=255)

                                                                   gATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGAt                         <  2:4195846/93‑1 (MQ=255)

                                                                     tCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCt                       >  2:63279/1‑93 (MQ=255)

                                                                     tCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCt                       <  2:5868011/93‑1 (MQ=255)

                                                                        tGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtttt                    >  1:2851950/1‑93 (MQ=255)

                                                                        tGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtttt                    <  1:6134610/93‑1 (MQ=255)

                                                                        tGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtttt                    <  1:1370296/93‑1 (MQ=255)

                                                                           gcTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 >  2:7250740/1‑93 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 <  1:7503430/93‑1 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 <  2:1573628/93‑1 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 >  1:4061404/1‑93 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 >  1:7326582/1‑93 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 >  2:2988488/1‑93 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt                 >  2:3199456/1‑93 (MQ=255)

                                                                             ttCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGttt               <  1:4541238/93‑1 (MQ=255)

                                                                              tCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTa              <  2:1468023/93‑1 (MQ=255)

                                                                               cGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTAt             <  2:3902246/93‑1 (MQ=255)

                                                                               cGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTAt             <  2:3405724/93‑1 (MQ=255)

                                                                                gCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATc            <  1:3527942/93‑1 (MQ=255)

                                                                                gCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATc            >  1:5366135/1‑93 (MQ=255)

                                                                                gCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATc            <  2:6062033/93‑1 (MQ=255)

                                                                                  cACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGc          >  2:2368898/1‑93 (MQ=255)

                                                                                   aCGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGcc         >  2:1341030/1‑93 (MQ=255)

                                                                                    cGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCt        <  1:1446172/93‑1 (MQ=255)

                                                                                    cGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCt        <  1:2210805/93‑1 (MQ=255)

                                                                                     gTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGcccg       >  1:2417312/1‑91 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg      >  1:3929455/1‑93 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg      >  1:5850382/1‑93 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg      <  2:7059625/93‑1 (MQ=255)

                                                                                          tGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTa  <  2:4870840/93‑1 (MQ=255)

CGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTA  >  NC_000913/2701461‑2701643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`