BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,719,936	A→G	Q655R (CAG→CGG)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,719,936	0	A	G	85.7%	699.0 / 103.5	300	Q655R (CAG→CGG)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (26/17); new base G (139/118); total (165/135)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 300 total aligned reads displayed.

CTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAAT  >  NC_000913/2719845‑2720025

 tGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGc                                                                                         <  2:7524453/93‑1 (MQ=255)

 tGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGc                                                                                         >  2:4363724/1‑93 (MQ=255)

    cccGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTc                                                                                      <  2:4256000/93‑1 (MQ=255)

     ccGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCt                                                                                     >  1:762407/1‑93 (MQ=255)

     ccGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCt                                                                                     >  1:2337740/1‑93 (MQ=255)

     ccGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCt                                                                                     <  2:1325857/93‑1 (MQ=255)

      cGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTg                                                                                    >  2:6032917/1‑93 (MQ=255)

      cGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTg                                                                                    >  2:2032226/1‑93 (MQ=255)

       gCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTgg                                                                                   >  1:3952837/1‑93 (MQ=255)

          aTCTGGTTATTCAGGGGATCGAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGc                                                                                >  1:4650150/1‑93 (MQ=255)

            cTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAg                                                                              <  2:4063703/93‑1 (MQ=255)

             tGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAgg                                                                             <  2:5847173/93‑1 (MQ=255)

              ggTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGt                                                                            >  1:1034735/1‑93 (MQ=255)

               gTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGtt                                                                           <  2:2468177/93‑1 (MQ=255)

               gTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGtt                                                                           <  2:6799379/93‑1 (MQ=255)

                 tATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTc                                                                         >  2:5409894/1‑93 (MQ=255)

                  aTTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGGTTcc                                                                        >  1:720264/1‑93 (MQ=255)

                   ttCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCa                                                                       <  1:51017/93‑1 (MQ=255)

                    tCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCaa                                                                      <  2:6146758/93‑1 (MQ=255)

                       ggggATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACtt                                                                   >  1:2713960/1‑93 (MQ=255)

                           aTCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGAtt                                                               <  1:7127156/93‑1 (MQ=255)

                            tCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTg                                                              >  1:7264995/1‑93 (MQ=255)

                             cAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGt                                                             >  1:930421/1‑93 (MQ=255)

                             cAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGt                                                             <  2:108809/93‑1 (MQ=255)

                              aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  1:2747258/1‑93 (MQ=255)

                              aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  1:5636967/1‑93 (MQ=255)

                              aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  2:6217664/1‑93 (MQ=255)

                              aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  2:4821329/1‑93 (MQ=255)

                               aaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCg                                                           >  1:5354298/1‑93 (MQ=255)

                                aaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGa                                                          <  1:5215584/93‑1 (MQ=255)

                                aaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGGGCAGGTTTCCAACTTGATTGTCGa                                                          >  1:6889237/1‑93 (MQ=255)

                                  gTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGAtt                                                        <  1:3143766/93‑1 (MQ=255)

                                   tAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTg                                                       <  1:5456848/93‑1 (MQ=255)

                                      aaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGGTTGTCGATTGccc                                                    >  1:4753727/1‑93 (MQ=255)

                                      aaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGccc                                                    >  1:6086529/1‑93 (MQ=255)

                                        aGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCgg                                                  >  1:4620133/1‑93 (MQ=255)

                                        aGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCgg                                                  >  1:6322204/1‑93 (MQ=255)

                                        aGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCgg                                                  >  1:6007515/1‑93 (MQ=255)

                                         gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 <  1:2949531/93‑1 (MQ=255)

                                         gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 >  2:5673596/1‑93 (MQ=255)

                                           ttCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGaaa                                               <  2:2944382/93‑1 (MQ=255)

                                           ttCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGaaa                                               >  1:2025450/1‑93 (MQ=255)

                                            tCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAAt                                              >  1:2363352/1‑93 (MQ=255)

                                             cGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAAtt                                             <  2:3877092/93‑1 (MQ=255)

                                              gTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTc                                            <  2:5917439/93‑1 (MQ=255)

                                              gTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTc                                            <  2:790673/93‑1 (MQ=255)

                                               tGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCa                                           <  2:2568573/93‑1 (MQ=255)

                                               tGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCa                                           <  1:6289842/93‑1 (MQ=255)

                                               tGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCa                                           <  1:3788201/93‑1 (MQ=255)

                                               tGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCa                                           <  2:5424204/93‑1 (MQ=255)

                                                 cgcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGc                                         <  1:7403558/93‑1 (MQ=255)

                                                  gcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCg                                        >  2:4621957/1‑93 (MQ=255)

                                                  gcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCg                                        <  2:500871/93‑1 (MQ=255)

                                                    gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      <  2:5613170/93‑1 (MQ=255)

                                                    gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      <  1:2545656/93‑1 (MQ=255)

                                                    gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      >  1:4011847/1‑93 (MQ=255)

                                                     cAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCt                                     <  2:2825957/93‑1 (MQ=255)

                                                       gTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTgg                                   >  1:2808236/1‑93 (MQ=255)

                                                       gTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTgg                                   >  1:3558065/1‑93 (MQ=255)

                                                        tGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGa                                  >  2:1464712/1‑93 (MQ=255)

                                                        tGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGa                                  <  1:1736985/93‑1 (MQ=255)

                                                          cgcTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGata                                <  2:1252371/93‑1 (MQ=255)

                                                           gcTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGatat                               >  2:176294/1‑93 (MQ=255)

                                                           gcTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGatat                               <  2:5789606/93‑1 (MQ=255)

                                                            cTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGTTAtt                              >  2:4523370/1‑93 (MQ=255)

                                                            cTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATAtt                              <  2:2082235/93‑1 (MQ=255)

                                                            cTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGCTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATAtt                              >  1:4328791/1‑93 (MQ=255)

                                                             tACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTc                             <  2:6290004/93‑1 (MQ=255)

                                                              aCGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTGCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCa                            <  1:3673983/93‑1 (MQ=255)

                                                                  ccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCt                        >  2:509985/1‑93 (MQ=255)

                                                                  ccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCt                        >  1:1822954/1‑93 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       <  1:3465865/93‑1 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  2:1838274/1‑93 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  2:7108134/1‑93 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  1:1279461/1‑93 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGGGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  1:6773651/1‑93 (MQ=255)

                                                                   cATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  1:330808/1‑93 (MQ=255)

                                                                     ttGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTg                     >  2:5215884/1‑93 (MQ=255)

                                                                     ttGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTg                     >  2:4754391/1‑93 (MQ=255)

                                                                      tGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGc                    <  2:3428237/93‑1 (MQ=255)

                                                                      tGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGc                    >  1:5031115/1‑93 (MQ=255)

                                                                       ggATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCt                   <  2:6661189/93‑1 (MQ=255)

                                                                        gATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTg                  >  1:1544480/1‑93 (MQ=255)

                                                                        gATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTg                  <  2:2901063/93‑1 (MQ=255)

                                                                          tGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGc                >  1:1123225/1‑93 (MQ=255)

                                                                          tGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGc                <  2:2409128/93‑1 (MQ=255)

                                                                           gTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCt               <  1:3128683/93‑1 (MQ=255)

                                                                            ttGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCtt              >  2:1549403/1‑93 (MQ=255)

                                                                            ttGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCtt              >  1:5250442/1‑93 (MQ=255)

                                                                            ttGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCtt              >  1:4159096/1‑93 (MQ=255)

                                                                            ttGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCtt              >  2:3261062/1‑93 (MQ=255)

                                                                              gCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCt            <  2:6493123/93‑1 (MQ=255)

                                                                               cAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTg           <  2:367200/93‑1 (MQ=255)

                                                                                aGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTgg          >  2:4952547/1‑93 (MQ=255)

                                                                                 ggCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTCATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGc         <  2:6848670/93‑1 (MQ=255)

                                                                                  gCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCa        <  1:4562886/93‑1 (MQ=255)

                                                                                  gCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCa        >  1:6489019/1‑93 (MQ=255)

                                                                                     tGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTg     <  2:7278069/93‑1 (MQ=255)

                                                                                     tGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTg     <  1:7112396/93‑1 (MQ=255)

                                                                                        gCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAAt  <  1:5717120/93‑1 (MQ=255)

CTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAAT  >  NC_000913/2719845‑2720025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`