BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	276,473	T→C	L179L (TTG→CTG)	yagD →	YagD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	276,473	0	T	C	86.4%	625.5 / 83.7	264	L179L (TTG→CTG)	yagD	YagD
Reads supporting (aligned to +/- strand): ref base T (18/18); new base C (128/100); total (146/118)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 269 total aligned reads displayed.

CGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTG  >  NC_000913/276381‑276565

cGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGt                                                                                              <  1:6363634/93‑1 (MQ=255)

  tttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTg                                                                                            >  1:6816305/1‑93 (MQ=255)

  tttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTg                                                                                            >  2:7354505/1‑93 (MQ=255)

  tttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTg                                                                                            >  1:164819/1‑93 (MQ=255)

  tttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTg                                                                                            >  2:4758182/1‑93 (MQ=255)

   ttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTgg                                                                                           <  2:1302463/93‑1 (MQ=255)

   ttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTgg                                                                                           >  1:828249/1‑93 (MQ=255)

    tCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGc                                                                                          >  2:2110955/1‑93 (MQ=255)

      aTCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCg                                                                                        >  1:3848978/1‑93 (MQ=255)

       tCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGa                                                                                       >  1:3398801/1‑93 (MQ=255)

       tCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGa                                                                                       >  1:4554394/1‑93 (MQ=255)

       tCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGa                                                                                       <  2:391305/93‑1 (MQ=255)

         gCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGc                                                                                     >  1:3903554/1‑93 (MQ=255)

         gCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGc                                                                                     >  1:2509829/1‑93 (MQ=255)

         gCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGc                                                                                     <  2:5658730/93‑1 (MQ=255)

           ccGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTg                                                                                   >  1:7241665/1‑93 (MQ=255)

           ccGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTg                                                                                   >  1:1925662/1‑93 (MQ=255)

           ccGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTg                                                                                   >  1:5100583/1‑93 (MQ=255)

            cgcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGt                                                                                  >  1:5358402/1‑93 (MQ=255)

             gcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTggt                                                                                 >  1:3711032/1‑91 (MQ=255)

              cgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTg                                                                                >  1:1588855/1‑93 (MQ=255)

                  tGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCg                                                                            >  1:4962877/1‑93 (MQ=255)

                   ggAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGATTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGc                                                                           <  1:4350812/93‑1 (MQ=255)

                    gAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCa                                                                          <  2:2914865/93‑1 (MQ=255)

                    gAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCa                                                                          >  2:1941786/1‑93 (MQ=255)

                      aGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCata                                                                        >  1:877407/1‑93 (MQ=255)

                      aGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCata                                                                        <  1:6180217/93‑1 (MQ=255)

                       gCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCatat                                                                       <  1:4322177/93‑1 (MQ=255)

                        ccTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATc                                                                      <  1:4472331/93‑1 (MQ=255)

                        ccTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATc                                                                      <  2:306461/93‑1 (MQ=255)

                          ttGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCcg                                                                    <  2:218166/93‑1 (MQ=255)

                          ttGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCcg                                                                    >  1:5161090/1‑93 (MQ=255)

                            gCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCcgcg                                                                  <  2:1779723/93‑1 (MQ=255)

                             cTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGt                                                                 <  1:6625931/93‑1 (MQ=255)

                                gATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTgcg                                                              <  2:2316267/93‑1 (MQ=255)

                                  tGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGcgcg                                                            <  1:3988360/93‑1 (MQ=255)

                                   gCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCgg                                                           <  1:5626184/93‑1 (MQ=255)

                                    ctggggCCGACCTGCTGGCCTGAGAACCCCTGCGGAATTTTTCCGAGATTGAGGGGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCggg                                                          <  1:2848081/91‑1 (MQ=255)

                                    ccGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCggg                                                          <  2:1019770/93‑1 (MQ=255)

                                     cGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGc                                                         >  2:725185/1‑93 (MQ=255)

                                      ggggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCg                                                        >  2:861085/1‑93 (MQ=255)

                                      ggggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCg                                                        <  2:3989756/93‑1 (MQ=255)

                                      ggggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCg                                                        <  2:4527110/93‑1 (MQ=255)

                                       gggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGt                                                       >  2:4311217/1‑93 (MQ=255)

                                         gCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTgg                                                     >  2:2538391/1‑93 (MQ=255)

                                         gCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTgg                                                     >  2:1590196/1‑93 (MQ=255)

                                            gATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtc                                                  >  1:1001885/1‑93 (MQ=255)

                                            gATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtc                                                  <  1:4711384/93‑1 (MQ=255)

                                            gATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtc                                                  <  2:404475/93‑1 (MQ=255)

                                             aTCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtct                                                 >  1:5996641/1‑93 (MQ=255)

                                             aTCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtct                                                 >  1:6547324/1‑93 (MQ=255)

                                             aTCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtct                                                 >  2:6130681/1‑93 (MQ=255)

                                              tCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTtctc                                                >  1:4918929/1‑93 (MQ=255)

                                               ctgctgGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCa                                               >  2:7240656/1‑93 (MQ=255)

                                                  ctgGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCAttt                                            >  2:2778782/1‑93 (MQ=255)

                                                   tgGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTa                                           <  2:5280496/93‑1 (MQ=255)

                                                   tgGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTCCTCATTTa                                           <  2:4860867/93‑1 (MQ=255)

                                                    ggCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTAc                                          <  1:5481433/93‑1 (MQ=255)

                                                     gCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTAcc                                         >  1:2443272/1‑93 (MQ=255)

                                                     gCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTAcc                                         >  1:5749401/1‑93 (MQ=255)

                                                      ccTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTAccc                                        <  1:2615071/93‑1 (MQ=255)

                                                       cTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCt                                       <  2:5682110/93‑1 (MQ=255)

                                                       cTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCt                                       >  1:3571132/1‑93 (MQ=255)

                                                        tGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTg                                      >  2:4594180/1‑93 (MQ=255)

                                                        tGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTg                                      >  2:5404485/1‑93 (MQ=255)

                                                        tGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTg                                      >  2:7017899/1‑93 (MQ=255)

                                                           gAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTgcgc                                   >  2:619832/1‑93 (MQ=255)

                                                           gAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTgcgc                                   >  1:2288347/1‑93 (MQ=255)

                                                           gAAACCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTgcgc                                   >  2:7447888/1‑93 (MQ=255)

                                                             aaCCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGa                                 >  2:6638227/1‑93 (MQ=255)

                                                             aaCCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGa                                 >  1:5901535/1‑93 (MQ=255)

                                                              aCCCTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGAc                                >  2:708540/1‑93 (MQ=255)

                                                               cccTGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACa                               >  1:2962147/1‑93 (MQ=255)

                                                                 cTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGc                             <  2:3776324/93‑1 (MQ=255)

                                                                  tGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCg                            >  2:3669532/1‑93 (MQ=255)

                                                                  tGCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCg                            >  2:3315450/1‑93 (MQ=255)

                                                                   gCCGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGa                           >  1:6613739/1‑93 (MQ=255)

                                                                    ccGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGaa                          <  1:1030558/93‑1 (MQ=255)

                                                                    ccGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGaa                          <  2:2605064/93‑1 (MQ=255)

                                                                    ccGAATTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCATGCGCGACAGCGaa                          <  1:7156399/93‑1 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       <  1:5692535/93‑1 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       >  2:2394466/1‑93 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       <  1:7089668/93‑1 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       >  2:6429359/1‑93 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       >  2:6059932/1‑93 (MQ=255)

                                                                       aaTTTTTCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAacac                       >  2:4948205/1‑93 (MQ=255)

                                                                        aTTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGTCGTGGTTCTCATTTACCCTGCGCGACAGCGAACAcc                      <  2:61699/93‑1 (MQ=255)

                                                                         tttttCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCt                     <  1:4130440/93‑1 (MQ=255)

                                                                         tttttCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCt                     >  2:698335/1‑93 (MQ=255)

                                                                          ttttCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTg                    >  1:2068980/1‑93 (MQ=255)

                                                                          ttttCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTg                    <  2:5075991/93‑1 (MQ=255)

                                                                          ttttCCGAGATTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTg                    >  2:3166953/1‑93 (MQ=255)

                                                                                gagaTTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGAc              <  1:1327276/93‑1 (MQ=255)

                                                                                gagaTTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGAc              <  2:3487932/93‑1 (MQ=255)

                                                                                gagaTTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGAc              >  1:7010/1‑93 (MQ=255)

                                                                                 agaTTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACg             >  2:3443257/1‑93 (MQ=255)

                                                                                   aTTGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGt           <  1:4040481/93‑1 (MQ=255)

                                                                                    ttGAGGCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTa          >  2:2627334/1‑93 (MQ=255)

                                                                                        ggCGCTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgcc      <  2:1247408/93‑1 (MQ=255)

                                                                                           gTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCt   <  1:3059986/93‑1 (MQ=255)

CGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTG  >  NC_000913/276381‑276565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`