BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,132	C→T	R229C (CGC→TGC)	emrA →	EmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,132	0	C	T	86.3%	710.6 / 68.8	263	R229C (CGC→TGC)	emrA	EmrA
Reads supporting (aligned to +/- strand): ref base C (16/20); new base T (120/107); total (136/127)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-02

Only 100 of 286 total aligned reads displayed.

CCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACTTTAA  >  NC_000913/2810040‑2810223

  gTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑Ccgctgc                                                                                           >  1:7452444/1‑93 (MQ=255)

  gTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑Ccgctgc                                                                                           <  1:5259442/93‑1 (MQ=255)

  gTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑Ccgccgc                                                                                           >  2:1475564/1‑93 (MQ=255)

  gTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑Ccgccgc                                                                                           >  1:831890/1‑93 (MQ=255)

  gTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑Ccgccgc                                                                                           >  2:6001145/1‑93 (MQ=255)

    gCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCtgcgc                                                                                         <  1:6103043/93‑1 (MQ=255)

    gCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCtgcgc                                                                                         >  2:3116664/1‑93 (MQ=255)

      aaCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCgg                                                                                       >  1:2737118/1‑93 (MQ=255)

      aaCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCgg                                                                                       >  1:4073525/1‑93 (MQ=255)

      aaCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCgg                                                                                       <  1:5702136/93‑1 (MQ=255)

      aaCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCgg                                                                                       >  1:189788/1‑93 (MQ=255)

       aCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGt                                                                                      >  1:7066289/1‑93 (MQ=255)

       aCAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGt                                                                                      >  2:4413250/1‑93 (MQ=255)

        cAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTa                                                                                     >  1:2538349/1‑93 (MQ=255)

         aGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTAc                                                                                    <  2:3793932/93‑1 (MQ=255)

          ggCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACa                                                                                   >  1:4894277/1‑93 (MQ=255)

          ggCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACa                                                                                   <  2:5275679/93‑1 (MQ=255)

           gCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAg                                                                                  <  1:5023039/93‑1 (MQ=255)

           gCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAg                                                                                  >  2:6923401/1‑93 (MQ=255)

             tGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTTCTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGTACAGcc                                                                                <  1:6144918/93‑1 (MQ=255)

             tGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGcc                                                                                >  1:584135/1‑93 (MQ=255)

               ccACCGAAGTACGTAACGCCTGGCTGGCGCTTGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCCCTGCGCGGTACAGCCTg                                                                              >  1:7420545/1‑93 (MQ=255)

               ccACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTg                                                                              <  2:1600173/93‑1 (MQ=255)

               ccACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTg                                                                              <  2:6976685/93‑1 (MQ=255)

                 aCCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTggg                                                                            <  1:4481055/93‑1 (MQ=255)

                 aCCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTggg                                                                            <  1:4716212/93‑1 (MQ=255)

                 aCCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGTACAGCCTggg                                                                            >  1:2560167/1‑93 (MQ=255)

                 aCCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGCACAGCCTggg                                                                            >  1:4362046/1‑93 (MQ=255)

                   cGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGgc                                                                          <  2:2794902/93‑1 (MQ=255)

                     aaGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGgcgc                                                                        >  1:2837329/1‑93 (MQ=255)

                      aGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCa                                                                       >  2:4763456/1‑93 (MQ=255)

                        tACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCaaa                                                                     <  2:1908441/93‑1 (MQ=255)

                           gTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTa                                                                  >  2:784946/1‑93 (MQ=255)

                            tAACGCCTGGGTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAg                                                                 <  1:1018390/93‑1 (MQ=255)

                            tAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAg                                                                 >  2:1068370/1‑93 (MQ=255)

                            tAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAg                                                                 >  2:5369545/1‑93 (MQ=255)

                             aaCGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGc                                                                >  2:1193457/1‑93 (MQ=255)

                              aCGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGcc                                                               >  2:2199982/1‑93 (MQ=255)

                               cGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGccc                                                              >  1:7410152/1‑93 (MQ=255)

                                gCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCa                                                             >  1:2172367/1‑93 (MQ=255)

                                gCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCa                                                             <  2:5664809/93‑1 (MQ=255)

                                 ccTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCaa                                                            <  2:7458347/93‑1 (MQ=255)

                                 ccTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCaa                                                            >  2:5107688/1‑93 (MQ=255)

                                  ctggctggCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAac                                                           <  2:2318982/93‑1 (MQ=255)

                                  ctggctggCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAac                                                           <  1:5384175/93‑1 (MQ=255)

                                  ctggctggCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAac                                                           >  2:294626/1‑93 (MQ=255)

                                   tggctggcGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAacg                                                          >  1:205313/1‑93 (MQ=255)

                                   tggctggcGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAacg                                                          >  1:6497577/1‑93 (MQ=255)

                                    ggctggcGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAacga                                                         <  2:1775655/93‑1 (MQ=255)

                                      ctggcGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAacgacg                                                       >  1:4253365/1‑93 (MQ=255)

                                         gcgcTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGAcgccg                                                    >  2:350958/1‑93 (MQ=255)

                                            cTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTg                                                 >  1:7204479/1‑93 (MQ=255)

                                             tGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGa                                                >  1:3982116/1‑93 (MQ=255)

                                               gAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATg                                              >  1:3332321/1‑93 (MQ=255)

                                               gAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATg                                              >  2:573576/1‑93 (MQ=255)

                                                aGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATgg                                             <  2:3313983/93‑1 (MQ=255)

                                                 gCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGc                                            <  1:6592500/93‑1 (MQ=255)

                                                 gCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGc                                            >  2:5870394/1‑93 (MQ=255)

                                                 gCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGc                                            >  2:7156602/1‑93 (MQ=255)

                                                  cGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCg                                           <  1:5066165/93‑1 (MQ=255)

                                                   gTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCgg                                          >  2:3152206/1‑93 (MQ=255)

                                                   gTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCgg                                          <  2:6510351/93‑1 (MQ=255)

                                                    tACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGt                                         <  1:4884273/93‑1 (MQ=255)

                                                    tACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGt                                         >  1:684789/1‑93 (MQ=255)

                                                    tACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGt                                         <  2:6290495/93‑1 (MQ=255)

                                                     aCTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTc                                        >  2:2061227/1‑93 (MQ=255)

                                                          tattatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTcc                                   <  2:1409478/93‑1 (MQ=255)

                                                           attatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCa                                  >  2:2043875/1‑93 (MQ=255)

                                                           attatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCa                                  >  2:859307/1‑93 (MQ=255)

                                                           attatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCa                                  <  1:5284944/93‑1 (MQ=255)

                                                            ttatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAg                                 >  1:496007/1‑93 (MQ=255)

                                                             tatCAGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGc                                >  2:6605910/1‑93 (MQ=255)

                                                                 aGTCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGccacc                            <  2:18431/93‑1 (MQ=255)

                                                                   tCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCaa                          <  1:2627121/93‑1 (MQ=255)

                                                                   tCCGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCaa                          <  2:6830850/93‑1 (MQ=255)

                                                                    ccGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAat                         <  1:6410499/93‑1 (MQ=255)

                                                                     cGATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAata                        >  1:6927154/1‑93 (MQ=255)

                                                                      gATGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAatat                       >  1:2509656/1‑93 (MQ=255)

                                                                        tGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgt                     <  2:414611/93‑1 (MQ=255)

                                                                        tGACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgt                     >  1:170838/1‑93 (MQ=255)

                                                                         gACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgtg                    <  1:2908218/93‑1 (MQ=255)

                                                                         gACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgtg                    <  1:3724943/93‑1 (MQ=255)

                                                                         gACCGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgtg                    <  2:4536469/93‑1 (MQ=255)

                                                                          aCCGGTTATGTCTCGCCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATAtgtg                    <  1:1189802/93‑1 (MQ=255)

                                                                           ccGGTTATGTCTC‑CCGCTGCGCGGGACAGCCTGGGGAGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATgtggg                  >  1:5185524/1‑93 (MQ=255)

                                                                           ccGGTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGGCGCCGCTGATGGCGGTCGTTCCAGCCACCAATATgtggg                  <  1:6214533/93‑1 (MQ=255)

                                                                            cGGTTATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATgtgggt                 >  1:3520871/1‑93 (MQ=255)

                                                                             ggTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATgtgggtg                >  1:3132716/1‑93 (MQ=255)

                                                                              gTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATgtgggtgg               >  2:985645/1‑93 (MQ=255)

                                                                               ttATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGa              >  2:3001919/1‑93 (MQ=255)

                                                                                tATGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGAt             <  1:3594710/93‑1 (MQ=255)

                                                                                  tGTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGc           >  2:7371264/1‑93 (MQ=255)

                                                                                  tGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGc           >  1:1372796/1‑93 (MQ=255)

                                                                                   gTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGcc          >  2:4946377/1‑93 (MQ=255)

                                                                                   gTCTC‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGcc          <  1:5885892/93‑1 (MQ=255)

                                                                                      tc‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAAc       >  2:6421740/1‑93 (MQ=255)

                                                                                      tc‑CCGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAAc       >  1:3112868/1‑93 (MQ=255)

                                                                                       c‑ccGCTGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACt      <  1:5508806/93‑1 (MQ=255)

                                                                                          cgctgcGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACttt    <  2:5549535/93‑1 (MQ=255)

                                                                                          cgctgcGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACttt    >  1:412271/1‑93 (MQ=255)

CCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCGTATTATCAGTCCGATGACCGGTTATGTCTC‑CCGCCGCGCGGTACAGCCTGGGGCGCAAATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACTTTAA  >  NC_000913/2810040‑2810223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`