BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,446	G→C	G196G (GGC→GGG)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,446	0	G	C	84.9%	757.9 / 110.3	271	G196G (GGC→GGG)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (25/16); new base C (127/103); total (152/119)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01

Only 100 of 280 total aligned reads displayed.

TCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGC  >  NC_000913/2819355‑2819538

tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGc                                                                                              >  2:4055699/1‑93 (MQ=255)

 cGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGccc                                                                                             >  1:6258754/1‑93 (MQ=255)

    gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                          >  1:372817/1‑93 (MQ=255)

     ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGcccccc                                                                                         >  1:1126003/1‑93 (MQ=255)

     ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCcccccc                                                                                         >  1:4971769/1‑93 (MQ=255)

       aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                       >  1:5933504/1‑93 (MQ=255)

       aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                       <  1:3243539/93‑1 (MQ=255)

        tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCaa                                                                                      <  1:2541290/93‑1 (MQ=255)

        tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaa                                                                                      >  1:6858103/1‑93 (MQ=255)

         cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaaa                                                                                     <  1:7103711/93‑1 (MQ=255)

           gCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAAtg                                                                                   <  1:2077646/93‑1 (MQ=255)

           gCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg                                                                                   <  2:6523190/93‑1 (MQ=255)

           gCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg                                                                                   >  2:102288/1‑93 (MQ=255)

            ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt                                                                                  <  2:1700690/93‑1 (MQ=255)

             cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt‑g                                                                                <  1:4371930/93‑1 (MQ=255)

              tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑gg                                                                               <  2:6452090/93‑1 (MQ=255)

               ggCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGt                                                                              <  1:3141195/93‑1 (MQ=255)

               ggCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGt                                                                              <  2:1229361/93‑1 (MQ=255)

               ggCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGt                                                                              >  1:2670892/1‑93 (MQ=255)

                gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTc                                                                             >  1:2891544/1‑93 (MQ=255)

                  ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGc                                                                           >  1:3441018/1‑93 (MQ=255)

                        aCTGCATGAAGACGATGTTCCAGATCTCAATGTATCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGAt                                                                     <  1:7230133/93‑1 (MQ=255)

                        aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGAt                                                                     >  2:2999905/1‑93 (MQ=255)

                        aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGAt                                                                     <  2:3073632/93‑1 (MQ=255)

                         cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATc                                                                    >  2:7083638/1‑93 (MQ=255)

                          tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCg                                                                   >  1:4588435/1‑93 (MQ=255)

                           gCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGt                                                                  >  1:5569412/1‑93 (MQ=255)

                            cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTa                                                                 >  1:2256430/1‑93 (MQ=255)

                              tGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTaga                                                               >  2:5885954/1‑93 (MQ=255)

                               gAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTagaa                                                              <  2:78567/93‑1 (MQ=255)

                                aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTagaag                                                             >  2:5259601/1‑93 (MQ=255)

                                 aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTagaaga                                                            >  2:2066241/1‑93 (MQ=255)

                                 aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTagaaga                                                            >  1:5800762/1‑93 (MQ=255)

                                  gACCATGTTCCAGATCTCAATGTAGCGGTCCCCCTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAAGTGGGTCGCCGTGATCGTagaaga                                                            <  1:6170185/93‑1 (MQ=255)

                                   aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGAtt                                                          >  1:79703/1‑93 (MQ=255)

                                   aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGAtt                                                          <  1:7005621/93‑1 (MQ=255)

                                    cGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGAttt                                                         <  2:134838/93‑1 (MQ=255)

                                     gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTc                                                        <  2:6089462/93‑1 (MQ=255)

                                     gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTc                                                        >  2:1982137/1‑93 (MQ=255)

                                       tGTTCCAGATCTCAGTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCgg                                                      >  2:5189740/1‑93 (MQ=255)

                                       tGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCgg                                                      >  1:5254261/1‑93 (MQ=255)

                                       tGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCgg                                                      <  2:3819991/93‑1 (MQ=255)

                                        gTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGt                                                     <  2:355772/93‑1 (MQ=255)

                                          tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                   >  1:511910/1‑93 (MQ=255)

                                          tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                   >  1:6092334/1‑93 (MQ=255)

                                          tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                   >  2:1841890/1‑93 (MQ=255)

                                           ccAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                                  >  1:1151242/1‑93 (MQ=255)

                                            cAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCAc                                                 <  2:4393137/93‑1 (MQ=255)

                                             aGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACg                                                <  1:1565974/93‑1 (MQ=255)

                                               aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                              >  2:5464284/1‑93 (MQ=255)

                                                 ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                            <  1:5413093/93‑1 (MQ=255)

                                                    aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                         >  2:5660814/1‑93 (MQ=255)

                                                     aTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                        <  1:7398199/93‑1 (MQ=255)

                                                     aTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGCCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                        >  2:5935270/1‑93 (MQ=255)

                                                      tGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACg                                       >  1:2770355/1‑93 (MQ=255)

                                                        tAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                     <  1:864570/93‑1 (MQ=255)

                                                        tAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                     <  2:5719098/93‑1 (MQ=255)

                                                        tAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                     >  2:6891131/1‑93 (MQ=255)

                                                         aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                    <  2:2857113/93‑1 (MQ=255)

                                                           cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa                                  <  1:635388/93‑1 (MQ=255)

                                                            ggTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                 <  2:5357689/93‑1 (MQ=255)

                                                             gTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgt                                <  2:5089058/93‑1 (MQ=255)

                                                              tCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACTAgtg                               <  1:3009786/93‑1 (MQ=255)

                                                               cGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGG‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtgt                              <  1:4230704/93‑1 (MQ=255)

                                                               cGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtgt                              >  2:2411511/1‑93 (MQ=255)

                                                                gCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTc                             >  1:4646155/1‑93 (MQ=255)

                                                                gCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCAGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTc                             <  1:234727/93‑1 (MQ=255)

                                                                gCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTc                             >  1:5727063/1‑93 (MQ=255)

                                                                 ccGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCa                            >  1:1387163/1‑93 (MQ=255)

                                                                 ccGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCa                            >  2:7360554/1‑93 (MQ=255)

                                                                   gTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                          >  2:1286341/1‑93 (MQ=255)

                                                                   gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                          >  1:4765774/1‑93 (MQ=255)

                                                                   gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                          >  2:531549/1‑93 (MQ=255)

                                                                   gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                          >  2:2573755/1‑93 (MQ=255)

                                                                    tcttctTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAccc                         >  1:4166263/1‑93 (MQ=255)

                                                                      ttcttcCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                       >  1:3919843/1‑93 (MQ=255)

                                                                      ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATTGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                       >  1:6423310/1‑93 (MQ=255)

                                                                      ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                       >  1:214960/1‑93 (MQ=255)

                                                                      ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                       >  2:6660905/1‑93 (MQ=255)

                                                                      ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                       <  1:35517/93‑1 (MQ=255)

                                                                       tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                      <  1:531475/93‑1 (MQ=255)

                                                                       tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                      >  2:4229413/1‑93 (MQ=255)

                                                                        cttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                     <  2:1463040/93‑1 (MQ=255)

                                                                        cttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                     <  2:2630198/93‑1 (MQ=255)

                                                                        cttcCGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                     <  2:488633/93‑1 (MQ=255)

                                                                           ccGGGCTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                  >  2:5528341/1‑93 (MQ=255)

                                                                                cTTCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAg             >  2:1527070/1‑93 (MQ=255)

                                                                                 ttCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt            >  1:1841784/1‑93 (MQ=255)

                                                                                  tCCCGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGtt           <  1:4114046/93‑1 (MQ=255)

                                                                                   cccGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTg          <  1:552152/93‑1 (MQ=255)

                                                                                   cccGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTg          >  1:1877144/1‑93 (MQ=255)

                                                                                    ccGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt         >  2:5555054/1‑93 (MQ=255)

                                                                                     cGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc        <  1:6698609/93‑1 (MQ=255)

                                                                                     cGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc        <  1:4801308/93‑1 (MQ=255)

                                                                                     cGGAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc        <  2:7191990/93‑1 (MQ=255)

                                                                                      ggAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa       <  1:1643250/93‑1 (MQ=255)

                                                                                       gAGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAg      >  1:4767535/1‑93 (MQ=255)

                                                                                        aGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa     <  2:4789932/93‑1 (MQ=255)

                                                                                        aGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa     <  1:2392645/93‑1 (MQ=255)

                                                                                        aGGCCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa     <  1:4000101/93‑1 (MQ=255)

TCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGT‑GGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGC  >  NC_000913/2819355‑2819538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`