BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,820,318	G→A	I115I (ATC→ATT)	oraA ←	OraA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,820,318	0	G	A	82.1%	825.6 / 122.2	318	I115I (ATC→ATT)	oraA	OraA
Reads supporting (aligned to +/- strand): ref base G (31/26); new base A (119/142); total (150/168)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

ACGCTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATAACCTT  >  NC_000913/2820228‑2820409

 cGCTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATg                                                                                          <  1:566676/93‑1 (MQ=255)

  gCTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGt                                                                                         <  1:137/93‑1 (MQ=255)

  gCTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGt                                                                                         >  2:2657996/1‑93 (MQ=255)

   cTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTc                                                                                        >  1:1176780/1‑93 (MQ=255)

   cTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTc                                                                                        <  1:6770306/93‑1 (MQ=255)

    tGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTca                                                                                       >  1:3707486/1‑93 (MQ=255)

    tGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTca                                                                                       <  1:6955487/93‑1 (MQ=255)

    tGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTca                                                                                       >  1:7415909/1‑93 (MQ=255)

      gATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTcaca                                                                                     <  2:6727028/93‑1 (MQ=255)

      gATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTcaca                                                                                     >  2:6629239/1‑93 (MQ=255)

        tCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACTAGTCAATGTCACAtt                                                                                   <  2:4735769/93‑1 (MQ=255)

        tCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACAtt                                                                                   >  1:2386854/1‑93 (MQ=255)

         cTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTc                                                                                  >  2:30996/1‑93 (MQ=255)

         cTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTc                                                                                  >  2:1428462/1‑93 (MQ=255)

          ttAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCa                                                                                 >  1:6635235/1‑93 (MQ=255)

          ttAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCa                                                                                 >  2:7446559/1‑93 (MQ=255)

           tAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCAc                                                                                >  2:1205962/1‑93 (MQ=255)

           tAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCAc                                                                                >  1:7241495/1‑93 (MQ=255)

             aCTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGc                                                                              >  1:7058783/1‑93 (MQ=255)

               tttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCAt                                                                            >  1:751992/1‑93 (MQ=255)

               tttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCAt                                                                            >  1:3134321/1‑93 (MQ=255)

               tttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCAt                                                                            >  1:1260629/1‑93 (MQ=255)

               tttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCAt                                                                            <  2:5087487/93‑1 (MQ=255)

                ttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATc                                                                           <  1:11067/93‑1 (MQ=255)

                ttttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATc                                                                           <  2:581627/93‑1 (MQ=255)

                 tttCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCg                                                                          <  2:78714/93‑1 (MQ=255)

                   tCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCt                                                                        <  2:3706407/93‑1 (MQ=255)

                    cTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCtt                                                                       <  2:2047953/93‑1 (MQ=255)

                     tGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCttt                                                                      >  1:4724018/1‑93 (MQ=255)

                     tGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCttt                                                                      <  1:6212657/93‑1 (MQ=255)

                     tGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCttt                                                                      >  1:211777/1‑93 (MQ=255)

                      gAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCtttt                                                                     >  1:2702365/1‑93 (MQ=255)

                      gAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCtttt                                                                     <  2:6041779/93‑1 (MQ=255)

                        aaaGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTc                                                                   >  2:4464861/1‑93 (MQ=255)

                         aaGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCt                                                                  >  1:3274862/1‑93 (MQ=255)

                         aaGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCt                                                                  <  1:5585529/93‑1 (MQ=255)

                         aaGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCt                                                                  >  2:1689390/1‑93 (MQ=255)

                          aGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTg                                                                 >  1:5172310/1‑93 (MQ=255)

                          aGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTg                                                                 <  2:3538661/93‑1 (MQ=255)

                           gACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGt                                                                >  2:5453274/1‑93 (MQ=255)

                           gACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGt                                                                >  1:6403884/1‑93 (MQ=255)

                           gACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGt                                                                <  1:2895394/93‑1 (MQ=255)

                            aCAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTc                                                               <  2:1598602/93‑1 (MQ=255)

                            aCAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTc                                                               <  1:3425365/93‑1 (MQ=255)

                               gTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCt                                                            >  1:1782471/1‑93 (MQ=255)

                                 tGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTc                                                          <  2:4921283/93‑1 (MQ=255)

                                 tGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTc                                                          <  1:1701255/93‑1 (MQ=255)

                                   gCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTcgc                                                        <  1:4308212/93‑1 (MQ=255)

                                     aaaGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATCCACGCATCGCTTTTTCTGTCGCTTCGCgg                                                      <  2:6568548/93‑1 (MQ=255)

                                        ggTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGaa                                                   >  1:6275943/1‑93 (MQ=255)

                                         gTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGaaa                                                  <  1:3885959/93‑1 (MQ=255)

                                          ttCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAAt                                                 >  2:5584939/1‑93 (MQ=255)

                                          ttCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAAt                                                 <  2:4489117/93‑1 (MQ=255)

                                           tCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATa                                                >  1:3509108/1‑93 (MQ=255)

                                            cGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATAc                                               <  1:5863934/93‑1 (MQ=255)

                                             gCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATAcc                                              <  2:7281621/93‑1 (MQ=255)

                                              ccATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCt                                             >  1:4392975/1‑93 (MQ=255)

                                                atatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCttt                                           <  2:1474151/93‑1 (MQ=255)

                                                atatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCttt                                           <  2:4681141/93‑1 (MQ=255)

                                                 tatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTc                                          <  2:4887415/93‑1 (MQ=255)

                                                 tatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTc                                          <  2:5055692/93‑1 (MQ=255)

                                                 tatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTc                                          <  2:7348635/93‑1 (MQ=255)

                                                 tatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTc                                          >  1:5295415/1‑93 (MQ=255)

                                                 tatTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTc                                          <  1:5452097/93‑1 (MQ=255)

                                                  atTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCt                                         >  1:4627465/1‑93 (MQ=255)

                                                  atTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCt                                         <  1:2640049/93‑1 (MQ=255)

                                                  atTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCt                                         >  1:1573994/1‑93 (MQ=255)

                                                   ttttCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTg                                        >  2:1798607/1‑93 (MQ=255)

                                                    tttCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGa                                       <  1:4510791/93‑1 (MQ=255)

                                                        gcgTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCa                                   <  2:6213314/93‑1 (MQ=255)

                                                        gcgTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCa                                   <  1:4375932/93‑1 (MQ=255)

                                                          gTCGCCTGATCGCGCGCCAGTGCGCACGAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGt                                 >  2:6421391/1‑93 (MQ=255)

                                                          gTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGt                                 <  2:2183494/93‑1 (MQ=255)

                                                          gTCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGt                                 <  1:5012291/93‑1 (MQ=255)

                                                           tCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGtt                                >  1:5804245/1‑93 (MQ=255)

                                                           tCGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGtt                                <  2:7198675/93‑1 (MQ=255)

                                                            cGCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTc                               <  1:969867/93‑1 (MQ=255)

                                                             gCCTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTcc                              >  2:5337638/1‑93 (MQ=255)

                                                              ccTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCt                             >  2:1075396/1‑93 (MQ=255)

                                                              ccTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCt                             >  1:6489053/1‑93 (MQ=255)

                                                               cTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTg                            <  2:3404609/93‑1 (MQ=255)

                                                               cTGATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTg                            <  2:2869793/93‑1 (MQ=255)

                                                                 gATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGc                          >  2:3158588/1‑93 (MQ=255)

                                                                 gATCGCGCGCCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGc                          >  2:5785950/1‑93 (MQ=255)

                                                                      cgcgcCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATa                     <  2:3901526/93‑1 (MQ=255)

                                                                      cgcgcCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATa                     <  2:5271747/93‑1 (MQ=255)

                                                                        cgcCAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATAcg                   <  1:852232/93‑1 (MQ=255)

                                                                           cAGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACgcgc                <  1:1928219/93‑1 (MQ=255)

                                                                            aGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCa               <  2:4013828/93‑1 (MQ=255)

                                                                            aGTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCa               <  1:6681163/93‑1 (MQ=255)

                                                                             gTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAg              <  2:2400390/93‑1 (MQ=255)

                                                                             gTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAg              >  1:2268850/1‑93 (MQ=255)

                                                                             gTGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAg              >  1:1406556/1‑93 (MQ=255)

                                                                              tGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAgg             >  2:115091/1‑93 (MQ=255)

                                                                              tGCGCACCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAgg             <  2:193088/93‑1 (MQ=255)

                                                                                   aCCAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCAt        >  2:5611947/1‑93 (MQ=255)

                                                                                    ccAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATa       <  1:6126803/93‑1 (MQ=255)

                                                                                    ccAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATa       <  1:2581901/93‑1 (MQ=255)

                                                                                     cAGTCAATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATaa      <  1:4432247/93‑1 (MQ=255)

                                                                                       gTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATAAcc    >  1:2948682/1‑93 (MQ=255)

ACGCTGGATCTTAACTTTTTCTGAAAAGACAGTTGGCAAAGGTTCGCCATATTTTCGCGTCGCCTGATCGCGCGCCAGTGCGCACCAGTCGATGTCACATTCACGCATCGCTTTTTCTGTCGCTTCGCGGGAAATACCTTTCTGATTCAGTTCCTGGCGAATACGCGCAGGTCCATAACCTT  >  NC_000913/2820228‑2820409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`