BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,033	G→T	R67R (CGC→CGA)	hycG ←	hydrogenase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,033	0	G	T	99.7%	1138.0 / ‑4.1	296	R67R (CGC→CGA)	hycG	hydrogenase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (156/139); minor base A (0/1); total (156/140)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

Only 100 of 296 total aligned reads displayed.

CAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGC  >  NC_000913/2841943‑2842122

cagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCg                                                                                         <  1:369689/93‑1 (MQ=255)

    gcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCt                                                                                     >  2:2554105/1‑93 (MQ=255)

      agGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                   >  2:4075635/1‑93 (MQ=255)

      agGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                   >  1:883000/1‑93 (MQ=255)

       ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                  >  2:6306357/1‑93 (MQ=255)

       ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                  >  2:1256279/1‑93 (MQ=255)

       ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                  >  1:6268701/1‑93 (MQ=255)

            tCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaaa                                                                             <  2:3962431/93‑1 (MQ=255)

              gCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                           >  1:2902619/1‑93 (MQ=255)

              gCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                           >  1:3890965/1‑93 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         >  2:761265/1‑93 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         <  2:5403386/93‑1 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         >  2:2947044/1‑93 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         >  2:7079230/1‑93 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         >  1:6512139/1‑93 (MQ=255)

                aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                         <  1:1303746/93‑1 (MQ=255)

                 ttGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                        >  1:1760248/1‑93 (MQ=255)

                   gCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggc                                                                      <  2:2010739/93‑1 (MQ=255)

                       gggTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                  <  1:930156/93‑1 (MQ=255)

                         gTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                <  1:784838/93‑1 (MQ=255)

                          tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCg                                                               <  2:3714912/93‑1 (MQ=255)

                          tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCg                                                               >  2:2371659/1‑93 (MQ=255)

                           gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGt                                                              <  1:4333720/93‑1 (MQ=255)

                            aCCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                             <  1:3074919/93‑1 (MQ=255)

                             ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                            <  2:917165/93‑1 (MQ=255)

                             ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                            <  2:7419878/93‑1 (MQ=255)

                              cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGc                                                           <  1:5988287/93‑1 (MQ=255)

                              cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGc                                                           <  2:1608703/93‑1 (MQ=255)

                                cgcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGa                                                         <  1:2378340/93‑1 (MQ=255)

                                  ccGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGCACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                       >  1:3944573/1‑93 (MQ=255)

                                  ccGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                       >  1:481738/1‑93 (MQ=255)

                                   cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaaa                                                      >  1:5846133/1‑93 (MQ=255)

                                    ggTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAat                                                     >  1:5602086/1‑93 (MQ=255)

                                    ggTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAat                                                     >  2:6691980/1‑93 (MQ=255)

                                     gTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                                    <  1:3369264/93‑1 (MQ=255)

                                      tAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAattt                                                   >  1:6835847/1‑93 (MQ=255)

                                        aaCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcg                                                 >  2:3782870/1‑93 (MQ=255)

                                          cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgat                                               >  1:7107033/1‑93 (MQ=255)

                                           aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatt                                              <  2:4861201/93‑1 (MQ=255)

                                           aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatt                                              >  2:2862381/1‑93 (MQ=255)

                                           aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatt                                              >  1:7465087/1‑93 (MQ=255)

                                            gTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgattt                                             <  2:3230428/93‑1 (MQ=255)

                                             tAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatttc                                            >  1:1921222/1‑93 (MQ=255)

                                               aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGc                                          >  2:1653947/1‑93 (MQ=255)

                                                  tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                       <  2:1295751/93‑1 (MQ=255)

                                                  tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                       <  1:1951348/93‑1 (MQ=255)

                                                  tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                       <  1:1597420/93‑1 (MQ=255)

                                                  tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                       <  1:6612802/93‑1 (MQ=255)

                                                    tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCg                                     >  2:4136205/1‑93 (MQ=255)

                                                       gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                  <  2:4307230/93‑1 (MQ=255)

                                                       gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                  >  1:2275757/1‑93 (MQ=255)

                                                       gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                  <  1:3795204/93‑1 (MQ=255)

                                                        cATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGc                                 >  1:3248889/1‑93 (MQ=255)

                                                          tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAg                               >  1:1844340/1‑93 (MQ=255)

                                                          tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAg                               >  1:2015312/1‑93 (MQ=255)

                                                           gACGCGGGGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgc                              >  1:3665229/1‑93 (MQ=255)

                                                              gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                           <  1:6455578/93‑1 (MQ=255)

                                                              gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                           <  2:7201887/93‑1 (MQ=255)

                                                              gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                           >  2:6777533/1‑93 (MQ=255)

                                                                 gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                        >  2:5637248/1‑93 (MQ=255)

                                                                 gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                        >  2:6047491/1‑93 (MQ=255)

                                                                 gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                        >  2:3891055/1‑93 (MQ=255)

                                                                  tGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCa                       <  1:7020481/93‑1 (MQ=255)

                                                                  tGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCa                       <  2:3220066/93‑1 (MQ=255)

                                                                  tGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCa                       >  1:5452926/1‑93 (MQ=255)

                                                                   gAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                      >  1:6358543/1‑93 (MQ=255)

                                                                     aGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                    >  1:4106672/1‑93 (MQ=255)

                                                                     aGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                    >  1:4492862/1‑93 (MQ=255)

                                                                      ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                   >  1:1066815/1‑93 (MQ=255)

                                                                       gAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCa                  <  2:366976/93‑1 (MQ=255)

                                                                        aaCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                 <  2:1138033/93‑1 (MQ=255)

                                                                          cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgc               >  1:7384534/1‑93 (MQ=255)

                                                                          cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgc               <  2:1822305/93‑1 (MQ=255)

                                                                            aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCgg             >  1:2644998/1‑93 (MQ=255)

                                                                            aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCgg             >  1:5795584/1‑93 (MQ=255)

                                                                             cTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGt            >  1:5517002/1‑93 (MQ=255)

                                                                             cTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGt            <  2:6494593/93‑1 (MQ=255)

                                                                              tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa           >  2:692099/1‑93 (MQ=255)

                                                                              tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa           >  1:3912146/1‑93 (MQ=255)

                                                                              tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa           >  1:4638504/1‑93 (MQ=255)

                                                                              tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa           >  1:4456912/1‑93 (MQ=255)

                                                                               ttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaa          <  1:1153821/93‑1 (MQ=255)

                                                                               ttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaa          <  2:4699681/93‑1 (MQ=255)

                                                                                 aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAAc        <  2:3325207/93‑1 (MQ=255)

                                                                                 aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAAc        <  1:2985284/93‑1 (MQ=255)

                                                                                 aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAAc        <  2:7009583/93‑1 (MQ=255)

                                                                                  aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa       <  1:3974357/93‑1 (MQ=255)

                                                                                  aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa       <  2:4996123/93‑1 (MQ=255)

                                                                                  aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa       <  1:5284230/93‑1 (MQ=255)

                                                                                   tGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACAt      <  2:7313684/93‑1 (MQ=255)

                                                                                    gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa     <  1:4283064/93‑1 (MQ=255)

                                                                                    gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa     <  2:583975/93‑1 (MQ=255)

                                                                                    gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa     >  1:7336973/1‑93 (MQ=255)

                                                                                    gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa     <  2:218959/93‑1 (MQ=255)

                                                                                      cGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg   <  2:6819202/93‑1 (MQ=255)

                                                                                      cGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg   >  1:2828392/1‑93 (MQ=255)

                                                                                      cGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg   <  2:3530925/93‑1 (MQ=255)

                                                                                       gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGc  >  1:4020527/1‑93 (MQ=255)

                                                                                       gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGc  <  1:3564982/93‑1 (MQ=255)

                                                                                       gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGc  >  2:3162067/1‑93 (MQ=255)

CAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGC  >  NC_000913/2841943‑2842122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`