BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,609	A→G	E395E (GAA→GAG)	yagF →	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,609	0	A	G	83.7%	562.0 / 103.1	257	E395E (GAA→GAG)	yagF	putative dehydratase
Reads supporting (aligned to +/- strand): ref base A (25/17); new base G (112/103); total (137/120)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01

Only 100 of 259 total aligned reads displayed.

GCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCC  >  NC_000913/283519‑283698

gCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtg                                                                                         <  2:7109133/93‑1 (MQ=255)

gCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtg                                                                                         <  1:4959185/93‑1 (MQ=255)

 ccGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtgg                                                                                        <  2:4581604/93‑1 (MQ=255)

  cGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtggt                                                                                       >  1:5572588/1‑93 (MQ=255)

  cGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAtggt                                                                                       <  2:875576/93‑1 (MQ=255)

   ggAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAtggtg                                                                                      >  2:3203716/1‑93 (MQ=255)

    gAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtggtgg                                                                                     <  2:4680184/93‑1 (MQ=255)

    gAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGtggtgg                                                                                     >  2:1390265/1‑93 (MQ=255)

    gAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAtggtgg                                                                                     <  1:2688718/93‑1 (MQ=255)

     aGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGc                                                                                    <  2:2594410/93‑1 (MQ=255)

      ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACTCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCa                                                                                   <  1:3345450/93‑1 (MQ=255)

      ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCa                                                                                   >  1:206103/1‑93 (MQ=255)

      ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCa                                                                                   <  1:4318309/93‑1 (MQ=255)

      ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCa                                                                                   <  2:3587179/93‑1 (MQ=255)

      ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCa                                                                                   <  1:1113614/93‑1 (MQ=255)

        tGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAgg                                                                                 >  1:5938023/1‑93 (MQ=255)

        tGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAgg                                                                                 <  1:5776975/93‑1 (MQ=255)

         gATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGc                                                                                >  2:3664446/1‑93 (MQ=255)

            gCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTc                                                                             <  2:4069128/93‑1 (MQ=255)

               ccACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGa                                                                          >  2:958238/1‑93 (MQ=255)

               ccACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGa                                                                          <  2:1774892/93‑1 (MQ=255)

               ccACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGa                                                                          <  1:4437989/93‑1 (MQ=255)

                cACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAg                                                                         >  1:3572231/1‑93 (MQ=255)

                cACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAg                                                                         >  2:4384573/1‑93 (MQ=255)

                    tGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGGGTCCGAGCGcc                                                                     >  1:5847479/1‑93 (MQ=255)

                    tGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGcc                                                                     >  1:6709897/1‑93 (MQ=255)

                      cgcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCgg                                                                   >  2:1169296/1‑93 (MQ=255)

                      cgcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCgg                                                                   <  1:3656386/93‑1 (MQ=255)

                       gcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCggg                                                                  >  1:2981301/1‑93 (MQ=255)

                         gACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGgcg                                                                >  2:6552087/1‑93 (MQ=255)

                          aCCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGgcgc                                                               >  1:6294598/1‑93 (MQ=255)

                          aCCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGgcgc                                                               >  1:3951529/1‑93 (MQ=255)

                           ccTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGgcgcg                                                              >  1:6097975/1‑93 (MQ=255)

                              cGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCtt                                                           >  1:2251348/1‑93 (MQ=255)

                               ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTc                                                          <  2:3405490/93‑1 (MQ=255)

                               ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCATGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTc                                                          >  2:5031354/1‑93 (MQ=255)

                                gCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTcc                                                         >  1:4110692/1‑93 (MQ=255)

                                 ccTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCg                                                        >  1:4654005/1‑93 (MQ=255)

                                  ctgctgCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGc                                                       >  1:184725/1‑93 (MQ=255)

                                   tgctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGcc                                                      >  1:1256355/1‑93 (MQ=255)

                                   tgctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGcc                                                      >  1:3545776/1‑93 (MQ=255)

                                   tgctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGcc                                                      <  2:4757836/93‑1 (MQ=255)

                                   tgctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGcc                                                      >  2:7459794/1‑93 (MQ=255)

                                     ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAg                                                    >  2:473806/1‑93 (MQ=255)

                                     ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAg                                                    >  2:187942/1‑93 (MQ=255)

                                      tgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGt                                                   <  2:2213035/93‑1 (MQ=255)

                                        cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGc                                                 >  2:5575602/1‑93 (MQ=255)

                                         aTCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGcc                                                >  1:1407358/1‑93 (MQ=255)

                                          tCTGGACGCCATGGCCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCt                                               <  1:5345892/93‑1 (MQ=255)

                                          tCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCt                                               <  1:7234961/93‑1 (MQ=255)

                                          tCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGAGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCt                                               >  1:5610589/1‑93 (MQ=255)

                                          tCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAAAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGccc                                               >  1:6222942/1‑92 (MQ=255)

                                            tGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTgc                                             <  2:2468773/93‑1 (MQ=255)

                                              gACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTgcgc                                           >  2:2331569/1‑93 (MQ=255)

                                                 gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAg                                        >  2:1652778/1‑93 (MQ=255)

                                                   cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCa                                      >  1:101689/1‑93 (MQ=255)

                                                    aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAg                                     >  2:2693557/1‑93 (MQ=255)

                                                     tgaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAgg                                    >  2:2149658/1‑93 (MQ=255)

                                                      gaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGa                                   <  1:3444381/93‑1 (MQ=255)

                                                       accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGAc                                  >  2:5098291/1‑93 (MQ=255)

                                                          gtgaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGc                               <  1:5009612/93‑1 (MQ=255)

                                                            gaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGt                             <  1:4750975/93‑1 (MQ=255)

                                                            gaccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGt                             >  2:2943569/1‑93 (MQ=255)

                                                             cccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTa                            <  1:3858146/92‑1 (MQ=255)

                                                              ccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTag                           <  1:7368044/93‑1 (MQ=255)

                                                              ccgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTag                           <  2:6303373/93‑1 (MQ=255)

                                                              ccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTag                           >  1:4374196/1‑93 (MQ=255)

                                                               cgGCCAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTaga                          <  1:698420/93‑1 (MQ=255)

                                                                  ccAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGcc                       >  2:1030776/1‑93 (MQ=255)

                                                                   cAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCg                      <  1:5737772/93‑1 (MQ=255)

                                                                   cAGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCAGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCg                      <  1:2879717/93‑1 (MQ=255)

                                                                   cAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCg                      >  2:6871131/1‑93 (MQ=255)

                                                                    aGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCgg                     >  2:2387258/1‑93 (MQ=255)

                                                                    aGACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCgg                     <  2:4121582/93‑1 (MQ=255)

                                                                    aGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCgg                     >  1:4857542/1‑93 (MQ=255)

                                                                     gACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGa                    >  1:1003665/1‑93 (MQ=255)

                                                                     gACGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGa                    <  2:2008051/93‑1 (MQ=255)

                                                                       cGGTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATg                  >  2:4330932/1‑93 (MQ=255)

                                                                        ggTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGa                 >  2:7145481/1‑93 (MQ=255)

                                                                         gTGGGCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAc                >  2:3323971/1‑93 (MQ=255)

                                                                            ggCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTg             <  1:6008596/93‑1 (MQ=255)

                                                                            ggCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTg             >  2:6446941/1‑93 (MQ=255)

                                                                             gCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGa            >  2:1240266/1‑93 (MQ=255)

                                                                             gCGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGa            >  1:1666906/1‑93 (MQ=255)

                                                                              cGAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGAt           <  1:6809389/93‑1 (MQ=255)

                                                                              cGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGAt           <  2:5518196/93‑1 (MQ=255)

                                                                               gagaACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATc          >  2:6956924/1‑93 (MQ=255)

                                                                                agaACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc         >  1:1760105/1‑93 (MQ=255)

                                                                                agaACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc         <  1:4691682/93‑1 (MQ=255)

                                                                                agaACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc         <  2:3832082/93‑1 (MQ=255)

                                                                                agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc         >  1:170280/1‑93 (MQ=255)

                                                                                 gaACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCt        <  2:5714794/93‑1 (MQ=255)

                                                                                  aaCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTg       <  1:1346852/93‑1 (MQ=255)

                                                                                   aCCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgc      <  1:3037320/93‑1 (MQ=255)

                                                                                   aCCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgc      <  1:3459571/93‑1 (MQ=255)

                                                                                   aCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgc      <  2:2119563/93‑1 (MQ=255)

                                                                                   aCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgc      >  2:3492447/1‑93 (MQ=255)

                                                                                      ttGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgc   >  2:7006025/1‑93 (MQ=255)

                                                                                       tGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgcc  >  2:5912811/1‑93 (MQ=255)

                                                                                       tGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgcc  <  1:723038/93‑1 (MQ=255)

GCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCC  >  NC_000913/283519‑283698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`