BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,900,224	T→C	W103R (TGG→CGG)	ygcE →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,900,224	0	T	C	85.4%	694.3 / 108.1	294	W103R (TGG→CGG)	ygcE	putative kinase
Reads supporting (aligned to +/- strand): ref base T (32/11); new base C (136/115); total (168/126)
Fisher's exact test for biased strand distribution p-value = 1.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 296 total aligned reads displayed.

AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTA  >  NC_000913/2900133‑2900316

agaagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTg                                                                                             >  1:4159360/1‑93 (MQ=255)

  aagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGc                                                                                           >  1:4610818/1‑93 (MQ=255)

  aagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGc                                                                                           >  2:763754/1‑93 (MQ=255)

   agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCa                                                                                          >  1:2307468/1‑93 (MQ=255)

   agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCa                                                                                          <  2:4973490/93‑1 (MQ=255)

     atatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAgg                                                                                        >  1:3397380/1‑93 (MQ=255)

      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGa                                                                                       >  1:6535627/1‑93 (MQ=255)

      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGa                                                                                       >  2:3940116/1‑93 (MQ=255)

      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGa                                                                                       >  1:5801550/1‑93 (MQ=255)

         tGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGc                                                                                    >  1:4303322/1‑93 (MQ=255)

           tCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAc                                                                                  >  2:688218/1‑93 (MQ=255)

             ggTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAcgc                                                                                <  2:4107876/93‑1 (MQ=255)

             ggTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAcgc                                                                                >  1:2864202/1‑93 (MQ=255)

               tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGt                                                                              >  2:2625393/1‑93 (MQ=255)

               tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGt                                                                              >  2:1719933/1‑93 (MQ=255)

                aTTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGtt                                                                             >  1:5275045/1‑93 (MQ=255)

                aTTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGtt                                                                             >  2:5658550/1‑93 (MQ=255)

                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTa                                                                            >  1:1747039/1‑93 (MQ=255)

                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  2:6387634/1‑93 (MQ=255)

                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  2:6793655/1‑93 (MQ=255)

                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  1:5998002/1‑93 (MQ=255)

                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            <  2:6134404/93‑1 (MQ=255)

                  tGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTac                                                                           <  1:826164/93‑1 (MQ=255)

                    gTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTacac                                                                         <  1:5605269/93‑1 (MQ=255)

                      cTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGc                                                                       <  2:1213053/93‑1 (MQ=255)

                      cTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGc                                                                       >  1:405336/1‑93 (MQ=255)

                       tGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGcc                                                                      >  2:2999813/1‑93 (MQ=255)

                        gggATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGccc                                                                     <  2:3392204/93‑1 (MQ=255)

                              catccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGa                                                               >  1:3787045/1‑93 (MQ=255)

                               atccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGaa                                                              >  2:3671841/1‑93 (MQ=255)

                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  1:4678502/1‑93 (MQ=255)

                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  1:3667617/1‑93 (MQ=255)

                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  2:1796828/1‑93 (MQ=255)

                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  2:232818/1‑93 (MQ=255)

                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             <  1:1163588/93‑1 (MQ=255)

                                 ccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAaca                                                            >  2:6017523/1‑93 (MQ=255)

                                  cGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAacac                                                           <  1:4827835/93‑1 (MQ=255)

                                      gctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTa                                                       >  1:7176876/1‑93 (MQ=255)

                                      gctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTa                                                       <  2:479726/93‑1 (MQ=255)

                                       ctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTaa                                                      <  2:815041/93‑1 (MQ=255)

                                          ccGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc                                                   <  1:5183289/93‑1 (MQ=255)

                                          ccGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc                                                   >  2:2407899/1‑93 (MQ=255)

                                           cGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                                                  >  2:4604158/1‑93 (MQ=255)

                                           cGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                                                  >  1:574452/1‑93 (MQ=255)

                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  2:739910/1‑93 (MQ=255)

                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  1:1473104/1‑93 (MQ=255)

                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  1:1026106/1‑93 (MQ=255)

                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  1:1220238/93‑1 (MQ=255)

                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  2:4899745/93‑1 (MQ=255)

                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                >  1:5512711/1‑93 (MQ=255)

                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                >  1:202203/1‑93 (MQ=255)

                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  2:2769659/93‑1 (MQ=255)

                                              gCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAc                                               <  1:2812147/93‑1 (MQ=255)

                                              gCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAc                                               >  1:5357502/1‑93 (MQ=255)

                                               cGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg                                              >  1:1300283/1‑93 (MQ=255)

                                                gTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGt                                             >  2:2831644/1‑93 (MQ=255)

                                                gTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGt                                             >  2:4225095/1‑93 (MQ=255)

                                                    ttGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa                                         <  2:1986164/93‑1 (MQ=255)

                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      <  2:891347/93‑1 (MQ=255)

                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      >  1:7299776/1‑93 (MQ=255)

                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      >  2:4339557/1‑93 (MQ=255)

                                                         aGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGt                                    <  1:4933335/93‑1 (MQ=255)

                                                          gCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTc                                   <  2:3597052/93‑1 (MQ=255)

                                                            cGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAc                                 >  2:5399111/1‑93 (MQ=255)

                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                >  1:2745349/1‑93 (MQ=255)

                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                <  1:3044975/93‑1 (MQ=255)

                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                >  2:344783/1‑93 (MQ=255)

                                                              aTGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACct                               >  2:5844908/1‑93 (MQ=255)

                                                               tGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACctc                              <  1:4392584/93‑1 (MQ=255)

                                                               tGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACctc                              <  1:2652262/93‑1 (MQ=255)

                                                                 gCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCtt                            <  1:5899423/93‑1 (MQ=255)

                                                                    cGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCttttt                         <  1:4735052/93‑1 (MQ=255)

                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        >  1:7209609/1‑93 (MQ=255)

                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        >  2:1414533/1‑93 (MQ=255)

                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        <  2:400365/93‑1 (MQ=255)

                                                                        tgctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     <  2:6058430/93‑1 (MQ=255)

                                                                        tgctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     >  1:1877471/1‑93 (MQ=255)

                                                                        tgctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     >  2:531609/1‑93 (MQ=255)

                                                                         gctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  2:323281/1‑93 (MQ=255)

                                                                         gctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  2:330725/1‑93 (MQ=255)

                                                                         gctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  1:2189956/1‑93 (MQ=255)

                                                                          ctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGtt                   <  2:3771873/93‑1 (MQ=255)

                                                                          ctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGtt                   <  2:6748096/93‑1 (MQ=255)

                                                                           tgcGCCATTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTa                  >  2:6989019/1‑93 (MQ=255)

                                                                            gcgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAt                 <  1:6397633/93‑1 (MQ=255)

                                                                            gcgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAt                 >  1:4552458/1‑93 (MQ=255)

                                                                             cgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCCCCTCTTTTTCGGGTTATc                <  1:1537478/93‑1 (MQ=255)

                                                                             cgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATc                <  1:7435989/93‑1 (MQ=255)

                                                                              gcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTctct               <  1:6971158/93‑1 (MQ=255)

                                                                              gcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCt               >  1:6807928/1‑93 (MQ=255)

                                                                               ccGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTg              <  1:1061182/93‑1 (MQ=255)

                                                                                 gTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  1:4710321/1‑93 (MQ=255)

                                                                                 gTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  2:5075510/1‑93 (MQ=255)

                                                                                 gTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  2:2280432/1‑93 (MQ=255)

                                                                                    gATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCa         <  1:6061455/93‑1 (MQ=255)

                                                                                    gATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCa         >  1:3966593/1‑93 (MQ=255)

                                                                                     aTTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCAt        >  1:994839/1‑93 (MQ=255)

                                                                                       tAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCg      >  2:6593004/1‑93 (MQ=255)

                                                                                        aGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGc     <  1:5091019/93‑1 (MQ=255)

                                                                                           tGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTa  <  2:5220773/93‑1 (MQ=255)

AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTA  >  NC_000913/2900133‑2900316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`