BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,074	A→G	intergenic (+109/‑3)	sdaB → / → b2798	L‑serine dehydratase/Exo

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,074	0	A	G	85.2%	687.6 / 113.7	304	intergenic (+109/‑3)	sdaB/b2798	L‑serine dehydratase/Exo
Reads supporting (aligned to +/- strand): ref base A (16/29); new base G (121/137); total (137/167)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 305 total aligned reads displayed.

TCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928982‑2929165

    tttGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGt                                                                                         <  2:7149148/93‑1 (MQ=255)

     tggCGGGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTg                                                                                        <  1:2679537/91‑1 (MQ=255)

      tGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTgg                                                                                       >  1:468267/1‑93 (MQ=255)

       gCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGc                                                                                      <  2:6777918/93‑1 (MQ=255)

       cgggggCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGc                                                                                      <  1:2946611/89‑1 (MQ=255)

         gAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTg                                                                                    >  1:5038317/1‑93 (MQ=255)

             ccTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCa                                                                                <  1:6498564/93‑1 (MQ=255)

             ccTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCa                                                                                <  1:868402/93‑1 (MQ=255)

              tcctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAt                                                                               <  1:3717150/91‑1 (MQ=255)

              ctctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAt                                                                               >  2:6108933/1‑93 (MQ=255)

                ctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAttt                                                                             <  1:3772444/93‑1 (MQ=255)

                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  2:5606605/1‑93 (MQ=255)

                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  2:5836818/1‑93 (MQ=255)

                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       <  2:2338405/93‑1 (MQ=255)

                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  1:6691226/1‑93 (MQ=255)

                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  2:3772432/93‑1 (MQ=255)

                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  1:7439239/93‑1 (MQ=255)

                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  2:7225724/93‑1 (MQ=255)

                         ttCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGt                                                                    <  1:453480/93‑1 (MQ=255)

                         ttCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGt                                                                    <  2:5513066/93‑1 (MQ=255)

                           ctcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCg                                                                  >  2:5114215/1‑93 (MQ=255)

                            tcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGa                                                                 <  2:6609970/93‑1 (MQ=255)

                             cATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGAt                                                                <  2:3574496/93‑1 (MQ=255)

                              aTCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATg                                                               >  1:1178248/1‑93 (MQ=255)

                              aTCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATg                                                               <  2:4254757/93‑1 (MQ=255)

                               tCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGc                                                              <  1:2147526/93‑1 (MQ=255)

                                ccAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCa                                                             <  2:5667448/93‑1 (MQ=255)

                                ccAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCa                                                             <  1:236454/93‑1 (MQ=255)

                                 cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                            <  2:1849173/93‑1 (MQ=255)

                                 cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                            <  1:6420791/93‑1 (MQ=255)

                                  aGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACt                                                           <  1:2462945/93‑1 (MQ=255)

                                  aGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACt                                                           <  1:7343821/93‑1 (MQ=255)

                                   gCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTg                                                          <  1:5596630/93‑1 (MQ=255)

                                      gTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAAt                                                       <  1:5342592/93‑1 (MQ=255)

                                      gTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAAt                                                       >  2:4174911/1‑93 (MQ=255)

                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      <  1:7222205/93‑1 (MQ=255)

                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      >  2:283504/1‑93 (MQ=255)

                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      >  2:2407257/1‑93 (MQ=255)

                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      <  2:6919250/93‑1 (MQ=255)

                                        aGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCt                                                     <  1:5367166/93‑1 (MQ=255)

                                         gCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCtt                                                    >  1:5112755/1‑93 (MQ=255)

                                          ccTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTa                                                   >  2:4804581/1‑93 (MQ=255)

                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  <  1:4847253/93‑1 (MQ=255)

                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  >  2:4861920/1‑93 (MQ=255)

                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  <  1:3972178/93‑1 (MQ=255)

                                            tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                                 >  1:7000778/1‑93 (MQ=255)

                                            tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                                 <  1:7190693/93‑1 (MQ=255)

                                             gTTCCGGCATCGAATGTTACCCTTATCTCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATCTGCTTATTGTCGATGCACTGAATCTTATtc                                                <  2:1618883/93‑1 (MQ=255)

                                             gTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtc                                                <  1:1736887/93‑1 (MQ=255)

                                              ttCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAttag                                               >  2:1243654/1‑91 (MQ=255)

                                                ccGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcgtc                                             <  2:668869/93‑1 (MQ=255)

                                                ccGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcgtc                                             <  2:4067994/93‑1 (MQ=255)

                                                  ggCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGc                                           >  1:5560625/1‑93 (MQ=255)

                                                  ggCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTAATTGTCGATGCACTGAATCTTATTCGTCGc                                           >  2:7434016/1‑93 (MQ=255)

                                                   gCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCa                                          <  2:1543467/93‑1 (MQ=255)

                                                   gCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCa                                          <  1:4314261/93‑1 (MQ=255)

                                                     aTCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAtt                                        >  1:1888702/1‑93 (MQ=255)

                                                     aTCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAtt                                        >  2:439085/1‑93 (MQ=255)

                                                      tCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTc                                       >  2:383679/1‑93 (MQ=255)

                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:298677/93‑1 (MQ=255)

                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:7011209/93‑1 (MQ=255)

                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:3347566/93‑1 (MQ=255)

                                                          aTGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGc                                   <  2:346431/93‑1 (MQ=255)

                                                           tGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGcc                                  >  1:2900322/1‑93 (MQ=255)

                                                           tGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGcc                                  <  1:7218934/93‑1 (MQ=255)

                                                               aCCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTc                              <  2:4293775/93‑1 (MQ=255)

                                                                cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                             >  1:3077954/1‑93 (MQ=255)

                                                                cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                             >  1:321452/1‑93 (MQ=255)

                                                                  cTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgg                           >  2:2675736/1‑93 (MQ=255)

                                                                  cTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgg                           >  2:5782190/1‑93 (MQ=255)

                                                                   ttATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                          >  2:3118915/1‑93 (MQ=255)

                                                                   ttATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                          >  2:6858254/1‑93 (MQ=255)

                                                                    tATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgggg                         >  1:4762688/1‑93 (MQ=255)

                                                                     aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                        <  2:6134181/93‑1 (MQ=255)

                                                                     aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                        >  2:2495057/1‑93 (MQ=255)

                                                                      tCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTc                       <  2:4443808/93‑1 (MQ=255)

                                                                      tCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTc                       >  2:394626/1‑93 (MQ=255)

                                                                       cGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCg                      <  1:131915/93‑1 (MQ=255)

                                                                        gCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGc                     >  1:349870/1‑93 (MQ=255)

                                                                        gCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGc                     <  1:1653678/93‑1 (MQ=255)

                                                                         ccTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTCCAGGGGTCGcc                    <  2:4658035/93‑1 (MQ=255)

                                                                          cTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGccc                   <  1:4891676/93‑1 (MQ=255)

                                                                           tGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                  >  2:7337227/1‑93 (MQ=255)

                                                                           tGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                  <  1:6937294/93‑1 (MQ=255)

                                                                            gATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtg                 >  1:3373522/1‑93 (MQ=255)

                                                                             aTCTTTAAGGGGGTGATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgt                >  2:6449273/1‑93 (MQ=255)

                                                                              tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg               <  1:305175/93‑1 (MQ=255)

                                                                              tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg               >  2:147036/1‑93 (MQ=255)

                                                                                tttAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTc             <  1:5183921/93‑1 (MQ=255)

                                                                                  tAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGa           <  2:121929/93‑1 (MQ=255)

                                                                                   aaGGGGGTTGTCGTCGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGaa          >  2:5187521/1‑93 (MQ=255)

                                                                                    aGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGaaa         <  2:3706434/93‑1 (MQ=255)

                                                                                     ggggggTATCGGGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  1:1018082/1‑93 (MQ=255)

                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        <  2:5059327/93‑1 (MQ=255)

                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  1:6090175/1‑93 (MQ=255)

                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        <  2:1350853/93‑1 (MQ=255)

                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  2:2696721/1‑93 (MQ=255)

                                                                                       gggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCt      <  1:6108956/93‑1 (MQ=255)

                                                                                         gTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGc    <  2:815197/93‑1 (MQ=255)

                                                                                           tATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCa  <  2:6406546/93‑1 (MQ=255)

TCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928982‑2929165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`