BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,996,557	T→C	K112K (AAA→AAG)	b2863 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,996,557	0	T	C	89.4%	703.3 / 62.6	265	K112K (AAA→AAG)	b2863	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/10); new base C (121/115); total (140/125)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 266 total aligned reads displayed.

CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTG  >  NC_000913/2996468‑2996649

cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTtc                                                                                           >  2:6016117/1‑93 (MQ=255)

cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           >  1:6124647/1‑93 (MQ=255)

cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           <  1:3281920/93‑1 (MQ=255)

cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           >  2:5378843/1‑93 (MQ=255)

 tATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtct                                                                                          >  2:6916189/1‑93 (MQ=255)

 tATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtct                                                                                          >  1:6634886/1‑93 (MQ=255)

  aTCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtctc                                                                                         <  1:2875808/93‑1 (MQ=255)

  aTCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtctc                                                                                         <  2:6462125/93‑1 (MQ=255)

    cccTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCg                                                                                       >  1:5111921/1‑93 (MQ=255)

     ccTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGt                                                                                      >  2:6600385/1‑93 (MQ=255)

      cTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTc                                                                                     <  2:837600/93‑1 (MQ=255)

      cTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTc                                                                                     >  2:1771601/1‑93 (MQ=255)

        cAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCaa                                                                                   <  2:6868913/93‑1 (MQ=255)

          aCCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACg                                                                                 <  2:4232525/93‑1 (MQ=255)

               aCAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAc                                                                            >  1:579048/1‑93 (MQ=255)

               aCAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAc                                                                            >  2:1334939/1‑93 (MQ=255)

                  gCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACttt                                                                         <  1:3996819/93‑1 (MQ=255)

                    atcatAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCg                                                                       >  1:2997183/1‑93 (MQ=255)

                     tcatAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgt                                                                      <  1:2716298/93‑1 (MQ=255)

                      catAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtg                                                                     >  1:6723027/1‑93 (MQ=255)

                       atAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtgt                                                                    <  2:7079510/93‑1 (MQ=255)

                        tAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtgtg                                                                   >  1:6904493/1‑93 (MQ=255)

                          gTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGtt                                                                 <  1:7325426/93‑1 (MQ=255)

                          gTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGtt                                                                 >  1:5505564/1‑93 (MQ=255)

                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                >  2:4904753/1‑93 (MQ=255)

                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                <  1:7287163/93‑1 (MQ=255)

                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                <  1:2364863/93‑1 (MQ=255)

                             ggTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTc                                                              >  2:3251392/1‑93 (MQ=255)

                             ggTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTc                                                              <  2:1496852/93‑1 (MQ=255)

                              gTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAAAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCt                                                             <  2:1581071/93‑1 (MQ=255)

                               tttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTg                                                            <  1:1241640/93‑1 (MQ=255)

                               tttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTg                                                            >  1:3122502/1‑93 (MQ=255)

                                ttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGt                                                           <  2:1925954/93‑1 (MQ=255)

                                   tGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTaa                                                        <  1:4958106/93‑1 (MQ=255)

                                    gCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAAt                                                       >  1:6286427/1‑93 (MQ=255)

                                     ccGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATg                                                      <  2:3473574/93‑1 (MQ=255)

                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    <  1:2037455/93‑1 (MQ=255)

                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGGTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    >  1:7374577/1‑93 (MQ=255)

                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    >  2:6082198/1‑93 (MQ=255)

                                        cTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTa                                                   <  2:5285457/93‑1 (MQ=255)

                                         tGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAg                                                  >  1:4522569/1‑93 (MQ=255)

                                          gTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGc                                                 <  2:2276316/93‑1 (MQ=255)

                                           ttttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCa                                                >  2:6303271/1‑93 (MQ=255)

                                           ttttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCa                                                >  2:7418306/1‑93 (MQ=255)

                                             ttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAt                                              <  2:5028417/93‑1 (MQ=255)

                                             ttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAt                                              >  1:4248851/1‑93 (MQ=255)

                                               gTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATcc                                            <  2:1145956/93‑1 (MQ=255)

                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  1:4859684/1‑93 (MQ=255)

                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  2:5143717/1‑93 (MQ=255)

                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           <  2:2589160/93‑1 (MQ=255)

                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           <  2:4022268/93‑1 (MQ=255)

                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  2:4364149/1‑93 (MQ=255)

                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  2:2677421/93‑1 (MQ=255)

                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  1:4665856/93‑1 (MQ=255)

                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          >  2:449249/1‑93 (MQ=255)

                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  2:1135582/93‑1 (MQ=255)

                                                  aTCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTg                                         >  2:5812768/1‑93 (MQ=255)

                                                    cTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGaa                                       <  1:5720259/93‑1 (MQ=255)

                                                    cTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGaa                                       <  2:3934439/93‑1 (MQ=255)

                                                       cGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAAttt                                    <  1:7170356/93‑1 (MQ=255)

                                                         aaaGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCg                                  >  2:4991363/1‑93 (MQ=255)

                                                           aGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTc                                >  2:3645965/1‑93 (MQ=255)

                                                            gTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCt                               <  2:5871122/93‑1 (MQ=255)

                                                             tAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCtt                              >  1:3919030/1‑93 (MQ=255)

                                                              aGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCttt                             <  2:2747529/93‑1 (MQ=255)

                                                               gAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTg                            <  2:5476227/93‑1 (MQ=255)

                                                               gAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTg                            <  2:2510981/93‑1 (MQ=255)

                                                                aaGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTgg                           <  1:3740365/93‑1 (MQ=255)

                                                                 aGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGt                          <  2:6766412/93‑1 (MQ=255)

                                                                    aaGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGtata                       <  2:3017446/93‑1 (MQ=255)

                                                                    aaGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGtata                       >  2:2550956/1‑93 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  1:4453313/1‑93 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      <  2:5636699/93‑1 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      <  2:1946785/93‑1 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:3175360/1‑93 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:4324314/1‑93 (MQ=255)

                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:2968060/1‑93 (MQ=255)

                                                                      gAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     >  1:103214/1‑93 (MQ=255)

                                                                      gAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTATTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     <  1:977826/93‑1 (MQ=255)

                                                                      gAATAAACGTTCCCCAGGGCTTCCCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     >  1:2141430/1‑93 (MQ=255)

                                                                         tAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  <  1:4758532/93‑1 (MQ=255)

                                                                         tAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  >  1:336272/1‑93 (MQ=255)

                                                                         tAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  >  1:5069272/1‑93 (MQ=255)

                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  1:6067180/93‑1 (MQ=255)

                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  2:1383873/93‑1 (MQ=255)

                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  2:5451550/93‑1 (MQ=255)

                                                                            aCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctct               >  1:5919197/1‑93 (MQ=255)

                                                                            aCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctct               >  2:1400900/1‑93 (MQ=255)

                                                                               ttCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTa            <  2:4528954/93‑1 (MQ=255)

                                                                                tCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAt           >  2:7488500/1‑93 (MQ=255)

                                                                                  cccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAttt         >  2:4011446/1‑93 (MQ=255)

                                                                                  cccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAttt         >  1:5532700/1‑93 (MQ=255)

                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        >  2:6173605/1‑93 (MQ=255)

                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        <  1:1771601/93‑1 (MQ=255)

                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        >  1:6497417/1‑93 (MQ=255)

                                                                                    cAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCa       >  2:4730446/1‑93 (MQ=255)

                                                                                     aGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAt      <  1:3514367/93‑1 (MQ=255)

                                                                                      gggTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAtt     <  1:7417378/93‑1 (MQ=255)

                                                                                      gggCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAtt     <  1:5166409/93‑1 (MQ=255)

                                                                                       ggTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAttt    <  2:5211375/93‑1 (MQ=255)

CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTG  >  NC_000913/2996468‑2996649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`