BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,377	A→G	V89A (GTT→GCT)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,377	0	A	G	83.4%	688.0 / 130.1	313	V89A (GTT→GCT)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base A (28/24); new base G (130/131); total (158/155)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 314 total aligned reads displayed.

TCTTCTCGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCAGGT  >  NC_000913/3070285‑3070467

tcttctCGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGaa                                                                                             <  1:4403614/93‑1 (MQ=255)

 cttctCGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAAc                                                                                            >  1:3746758/1‑93 (MQ=255)

   tctcGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑g                                                                                         >  2:5862784/1‑93 (MQ=255)

    ctcGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑gg                                                                                        >  1:1020554/1‑93 (MQ=255)

    ctcGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑gg                                                                                        <  2:4524208/93‑1 (MQ=255)

     tcGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑ggg                                                                                       <  1:5721634/93‑1 (MQ=255)

      cGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGt                                                                                      <  1:6157714/93‑1 (MQ=255)

       gCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGtt                                                                                     >  1:2858173/1‑93 (MQ=255)

         cTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAg                                                                                   >  2:589067/1‑93 (MQ=255)

          tttGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGa                                                                                  >  2:3981199/1‑93 (MQ=255)

          tttGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGa                                                                                  >  1:7082197/1‑93 (MQ=255)

           ttgttgAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGAc                                                                                 >  1:6866673/1‑93 (MQ=255)

            tgttgAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACa                                                                                <  1:4721588/93‑1 (MQ=255)

            tgttgAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACa                                                                                >  1:906986/1‑93 (MQ=255)

              ttgAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGt                                                                              <  1:3346045/93‑1 (MQ=255)

                gAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGttt                                                                            >  2:6844337/1‑93 (MQ=255)

                gAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGttt                                                                            >  2:436805/1‑93 (MQ=255)

                gAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGttt                                                                            <  2:4228706/93‑1 (MQ=255)

                gAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGttt                                                                            >  1:4593803/1‑93 (MQ=255)

                 aaGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTg                                                                           >  2:1317199/1‑93 (MQ=255)

                  aGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGt                                                                          >  1:2780600/1‑93 (MQ=255)

                  aGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGt                                                                          >  2:2203904/1‑93 (MQ=255)

                   gCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACTTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCCGGGGTTAGACAGTTTGt                                                                          >  1:2418796/1‑93 (MQ=255)

                     gAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCtt                                                                       <  2:3426396/93‑1 (MQ=255)

                         gTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAg                                                                   >  2:4705669/1‑93 (MQ=255)

                         gTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAg                                                                   >  2:6377167/1‑93 (MQ=255)

                          ttttCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAgg                                                                  >  2:4846860/1‑93 (MQ=255)

                           tttCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGt                                                                 >  2:1368518/1‑93 (MQ=255)

                           tttCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGt                                                                 <  1:5678948/93‑1 (MQ=255)

                            ttCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTa                                                                <  2:7203302/93‑1 (MQ=255)

                             tCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAg                                                               >  1:1852685/1‑93 (MQ=255)

                              ccAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGt                                                              >  2:4928429/1‑93 (MQ=255)

                              ccAGAACAACCAGTCCACCTTCAGCAACGTCACCGCCGTCGAGGTATTCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGt                                                              <  1:5548778/93‑1 (MQ=255)

                                 gAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaa                                                           >  1:404609/1‑93 (MQ=255)

                                 gAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaa                                                           >  2:1975088/1‑93 (MQ=255)

                                  aacaacCAGTTCCCCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaac                                                          <  1:2372664/93‑1 (MQ=255)

                                  aacaacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaac                                                          <  2:3175588/93‑1 (MQ=255)

                                   acaacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaaca                                                         >  1:7295900/1‑93 (MQ=255)

                                    caacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaa                                                        <  2:670952/93‑1 (MQ=255)

                                    caacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaa                                                        <  2:1936942/93‑1 (MQ=255)

                                    caacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaa                                                        <  2:4165862/93‑1 (MQ=255)

                                    caacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaa                                                        <  1:2277683/93‑1 (MQ=255)

                                     aacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaac                                                       >  2:7355758/1‑93 (MQ=255)

                                     aacCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTaacaac                                                       <  1:5142179/93‑1 (MQ=255)

                                      acCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAAcc                                                      <  2:2057944/93‑1 (MQ=255)

                                        cAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCgg                                                    >  1:4329924/1‑93 (MQ=255)

                                          gTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGgca                                                  <  1:5023335/93‑1 (MQ=255)

                                          gTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGgca                                                  >  2:1508015/1‑93 (MQ=255)

                                          gTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGgca                                                  <  1:5619630/93‑1 (MQ=255)

                                           ttCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGgcag                                                 <  2:4070996/93‑1 (MQ=255)

                                            tCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGgcagc                                                <  1:1264108/93‑1 (MQ=255)

                                              aCCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGcagcag                                              >  2:3041832/1‑93 (MQ=255)

                                               ccTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCaga                                             >  1:3150584/1‑93 (MQ=255)

                                               ccTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCaga                                             <  1:2679085/93‑1 (MQ=255)

                                               ccTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCaga                                             >  2:7037828/1‑93 (MQ=255)

                                               ccTTCACCACCGTCAGCGCGGTCGAGTTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCaga                                             <  1:3185714/93‑1 (MQ=255)

                                                 ttCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑TGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCagaga                                           <  1:6396078/93‑1 (MQ=255)

                                                  tCAGCAGCGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCagagag                                          <  2:2750751/93‑1 (MQ=255)

                                                  tCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCagagag                                          <  2:3773149/93‑1 (MQ=255)

                                                    aGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGaa                                        <  1:3035981/93‑1 (MQ=255)

                                                     gCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAt                                       <  1:3491869/93‑1 (MQ=255)

                                                     gCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAt                                       >  1:3982595/1‑93 (MQ=255)

                                                     gCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAt                                       >  1:2142442/1‑93 (MQ=255)

                                                     gCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAt                                       >  1:1605884/1‑93 (MQ=255)

                                                        aCGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAttct                                    >  2:6085730/1‑93 (MQ=255)

                                                        aCGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAttct                                    >  1:7399188/1‑93 (MQ=255)

                                                         cGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAttctt                                   <  1:665238/93‑1 (MQ=255)

                                                          gTCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAAttcttc                                  <  1:5791526/93‑1 (MQ=255)

                                                           tCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCg                                 >  2:2845138/1‑93 (MQ=255)

                                                           tCAACGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCg                                 >  2:6286457/1‑93 (MQ=255)

                                                           tCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCg                                 <  2:1092521/93‑1 (MQ=255)

                                                             aaCGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGtt                               <  1:4193802/93‑1 (MQ=255)

                                                             aaCGCCGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTCCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGtt                               <  1:6207097/93‑1 (MQ=255)

                                                                  cGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACt                          <  2:1230500/93‑1 (MQ=255)

                                                                  cGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACt                          <  2:1737088/93‑1 (MQ=255)

                                                                  cGTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACt                          <  1:6452099/93‑1 (MQ=255)

                                                                   gTCGAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTc                         <  2:5197545/93‑1 (MQ=255)

                                                                      gAGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGcc                      >  1:1349309/1‑93 (MQ=255)

                                                                       aGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCt                     >  2:2409591/1‑93 (MQ=255)

                                                                       aGGTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCt                     <  1:5294864/93‑1 (MQ=255)

                                                                        ggTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCtt                    <  2:2350561/93‑1 (MQ=255)

                                                                        ggTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCtt                    <  2:1815370/93‑1 (MQ=255)

                                                                         gTAATCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTc                   <  2:2557868/93‑1 (MQ=255)

                                                                            aTCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGt                <  1:3644144/93‑1 (MQ=255)

                                                                             tCTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTa               >  1:1494981/1‑93 (MQ=255)

                                                                              cTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAg              >  2:5600591/1‑93 (MQ=255)

                                                                              cTTTAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAg              <  1:5941148/93‑1 (MQ=255)

                                                                               tttAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAgg             <  2:6964827/93‑1 (MQ=255)

                                                                                ttAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGga            >  2:3705618/1‑93 (MQ=255)

                                                                                ttAACCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGga            <  2:6479390/93‑1 (MQ=255)

                                                                                ttAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGga            >  1:2566722/1‑93 (MQ=255)

                                                                                  aaCCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGgacg          >  1:1069252/1‑93 (MQ=255)

                                                                                   aCCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGgacga         >  1:4824874/1‑93 (MQ=255)

                                                                                   aCCAGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGgacga         >  1:3837496/1‑93 (MQ=255)

                                                                                     cAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGAcc       >  2:3642828/1‑93 (MQ=255)

                                                                                      aGACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGAccc      >  1:422092/1‑93 (MQ=255)

                                                                                       gACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCa     >  2:763714/1‑93 (MQ=255)

                                                                                       gACGAGCC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCa     >  2:838456/1‑93 (MQ=255)

                                                                                       gACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCa     >  2:4407446/1‑93 (MQ=255)

                                                                                        aCGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCAg    <  2:4624759/93‑1 (MQ=255)

TCTTCTCGCCTTTGTTGAAGCGAACGTTTTCCAGAACAACCAGTTCACCTTCAGCAACGTCAACGCCGTCGAGGTAATCTTTAACCAGACGAACC‑GGGTTAGACAGTTTGTCTTTCAGGTAGTTAACAACCGGCAGCAGAGAGAATTCTTCGTTGTACTCGCCTTCGGTAGGACGACCCAGGT  >  NC_000913/3070285‑3070467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`