BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,074,951	C→T	G80S (GGC→AGC)	b2931 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,074,951	0	C	T	87.9%	915.2 / 67.6	306	G80S (GGC→AGC)	b2931	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (15/22); new base T (119/150); total (134/172)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 314 total aligned reads displayed.

ACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCT  >  NC_000913/3074860‑3075040

accaccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTgccg                                                                                          >  2:1641244/1‑93 (MQ=255)

  caccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTgctgcc                                                                                        <  2:6593063/93‑1 (MQ=255)

   accCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCa                                                                                       >  1:5946634/1‑93 (MQ=255)

    nncgcgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAg                                                                                      <  2:2125430/91‑1 (MQ=255)

    cccGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAg                                                                                      <  2:6231480/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:6762199/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:3772028/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:2237817/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  2:1428399/93‑1 (MQ=255)

            ggTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACg                                                                              >  2:5844952/1‑93 (MQ=255)

             gTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGc                                                                             >  1:1996312/1‑93 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          >  2:7130863/1‑93 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          <  1:5494199/93‑1 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          <  2:5587553/93‑1 (MQ=255)

                   tGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGc                                                                       >  1:3314506/1‑93 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGa                                                                     <  2:90627/93‑1 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGa                                                                     <  2:1284118/93‑1 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                                     <  1:6395441/93‑1 (MQ=255)

                      cgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAt                                                                    >  2:2755307/1‑93 (MQ=255)

                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGc                                                                  >  1:5324452/1‑93 (MQ=255)

                          gTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTc                                                                >  1:1890943/1‑93 (MQ=255)

                          gTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTc                                                                >  1:2956549/1‑93 (MQ=255)

                           ttAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCa                                                               >  2:4646718/1‑93 (MQ=255)

                           ttAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCa                                                               >  1:875270/1‑93 (MQ=255)

                            tAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCAt                                                              >  1:3187634/1‑93 (MQ=255)

                              aTGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATaa                                                            >  2:4164959/1‑93 (MQ=255)

                               tGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATaaa                                                           >  2:4420547/1‑93 (MQ=255)

                                ggCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAAc                                                          >  2:2868339/1‑93 (MQ=255)

                                ggCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAAc                                                          <  2:4041193/93‑1 (MQ=255)

                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACat                                                        <  2:189882/93‑1 (MQ=255)

                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACat                                                        <  2:3533796/93‑1 (MQ=255)

                                   gTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACata                                                       >  2:1720751/1‑93 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  1:950749/93‑1 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  1:5797089/93‑1 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      >  2:4567133/1‑93 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  2:5653191/93‑1 (MQ=255)

                                      aTCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTg                                                    <  2:4873919/93‑1 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCGCACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgt                                                 <  2:3302193/93‑1 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgt                                                 >  2:768825/1‑93 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATANTGCgt                                                 >  1:6491833/1‑93 (MQ=255)

                                          aTAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtg                                                >  2:3147547/1‑93 (MQ=255)

                                          aTAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtg                                                <  2:1077066/93‑1 (MQ=255)

                                           tAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtgg                                               <  2:6982817/93‑1 (MQ=255)

                                           tAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtgg                                               <  1:413720/93‑1 (MQ=255)

                                               gcCAATGCCCATCGGACCTGCACGCGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGtgtg                                           <  1:1389032/93‑1 (MQ=255)

                                                 cAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGAt                                         <  1:100033/93‑1 (MQ=255)

                                                  aaTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATa                                        <  1:5085171/93‑1 (MQ=255)

                                                  aaTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATa                                        >  1:4338852/1‑93 (MQ=255)

                                                   aTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATaa                                       <  1:2530368/93‑1 (MQ=255)

                                                   aTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATaa                                       >  2:6343996/1‑93 (MQ=255)

                                                    tGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                                      >  1:3621986/1‑93 (MQ=255)

                                                    tGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                                      >  1:4993983/1‑93 (MQ=255)

                                                      cccATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTg                                    >  1:4721737/1‑93 (MQ=255)

                                                      cccATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTg                                    <  2:1203741/93‑1 (MQ=255)

                                                       ccATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTgg                                   <  1:3030810/93‑1 (MQ=255)

                                                        cATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGc                                  >  1:2653585/1‑93 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 >  2:3988925/1‑93 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  2:3839165/93‑1 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  1:819129/93‑1 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  2:5914078/93‑1 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                >  1:3702499/1‑93 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                <  1:2435008/93‑1 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                <  1:7000012/93‑1 (MQ=255)

                                                              aCCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATa                            <  1:603342/93‑1 (MQ=255)

                                                               ccTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATaa                           <  1:7114421/93‑1 (MQ=255)

                                                               ccTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATaa                           >  1:7400190/1‑93 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          <  2:820820/93‑1 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          >  1:1276984/1‑93 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          <  2:2370293/93‑1 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          >  1:4383742/1‑93 (MQ=255)

                                                                 tGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                         >  1:4908654/1‑93 (MQ=255)

                                                                    acacGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCAcc                      <  1:5881084/93‑1 (MQ=255)

                                                                      acGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaa                    <  1:6814845/93‑1 (MQ=255)

                                                                       cGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat                   <  1:4605612/93‑1 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                >  2:5260152/1‑93 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                <  2:5377373/93‑1 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                <  1:2880470/93‑1 (MQ=255)

                                                                           agcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg               <  1:7295953/93‑1 (MQ=255)

                                                                           agcagTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg               >  2:6379933/1‑93 (MQ=255)

                                                                              agTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcag            >  2:4762074/1‑93 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTTATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           >  1:6226764/1‑93 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           <  2:2512083/93‑1 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           >  2:4948766/1‑93 (MQ=255)

                                                                                tGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagca          >  2:5505701/1‑93 (MQ=255)

                                                                                tGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagca          >  2:360672/1‑93 (MQ=255)

                                                                                 gCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcac         >  2:7394640/1‑93 (MQ=255)

                                                                                  cAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcaca        <  2:6727135/93‑1 (MQ=255)

                                                                                  cAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcaca        <  2:4321759/93‑1 (MQ=255)

                                                                                   aaTATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACAt       >  1:1610235/1‑93 (MQ=255)

                                                                                    atatTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATa      <  1:1673694/93‑1 (MQ=255)

                                                                                    atatTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATa      <  2:3704421/93‑1 (MQ=255)

                                                                                     tatTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAg     <  1:2920094/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:3893573/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:3462125/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    >  2:2269154/1‑93 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:745745/93‑1 (MQ=255)

                                                                                       ttGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGc   >  2:5168553/1‑93 (MQ=255)

                                                                                        tgctgcCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  <  2:6101249/93‑1 (MQ=255)

                                                                                        tgctgcCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  >  2:7263706/1‑93 (MQ=255)

                                                                                        tGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  <  2:3273205/93‑1 (MQ=255)

ACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCT  >  NC_000913/3074860‑3075040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`