BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,069	C→G	V136L (GTC→CTC)	yagV ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,069	0	C	G	85.7%	844.1 / 112.4	293	V136L (GTC→CTC)	yagV	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (19/23); new base G (105/146); total (124/169)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 295 total aligned reads displayed.

CGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAA  >  NC_000913/303978‑304159

cGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAcc                                                                                           <  1:6561251/93‑1 (MQ=255)

 gTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCt                                                                                          <  2:5413084/93‑1 (MQ=255)

 gTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCt                                                                                          >  2:781222/1‑93 (MQ=255)

 gTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCt                                                                                          <  2:2686977/93‑1 (MQ=255)

  tactacCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTc                                                                                         <  2:4545587/93‑1 (MQ=255)

    ctacCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTcgc                                                                                       <  2:1542729/93‑1 (MQ=255)

    ctacCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTcgc                                                                                       >  1:6424572/1‑93 (MQ=255)

         aGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAAt                                                                                  >  1:149873/1‑93 (MQ=255)

         aGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAAt                                                                                  <  1:3299131/93‑1 (MQ=255)

           aaTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATga                                                                                <  1:5058682/93‑1 (MQ=255)

           aaTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATga                                                                                >  2:4016644/1‑93 (MQ=255)

           aaTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATga                                                                                <  1:793281/93‑1 (MQ=255)

            aTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATgag                                                                               <  2:1273891/93‑1 (MQ=255)

            aTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATgag                                                                               >  1:281481/1‑93 (MQ=255)

             tGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATgaga                                                                              <  1:3607418/93‑1 (MQ=255)

               gTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGAcc                                                                            >  2:4446194/1‑93 (MQ=255)

                  tCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCgg                                                                         >  2:4162787/1‑93 (MQ=255)

                   ccATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGgt                                                                        <  1:7109897/93‑1 (MQ=255)

                     acccccaccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGgtag                                                                      <  2:7007940/89‑1 (MQ=255)

                      tCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGgtagt                                                                     >  2:4257664/1‑93 (MQ=255)

                       caccaccacCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGgtagta                                                                    <  2:4970569/93‑1 (MQ=255)

                        accaccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGtagtag                                                                   >  2:5495537/1‑93 (MQ=255)

                         ccaccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAgc                                                                  >  2:6228230/1‑93 (MQ=255)

                           accaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAgcgc                                                                <  1:6500539/93‑1 (MQ=255)

                           accaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAgcgc                                                                <  1:4548215/93‑1 (MQ=255)

                            ccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCt                                                               >  1:1025717/1‑93 (MQ=255)

                             caccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTc                                                              <  2:1365974/93‑1 (MQ=255)

                                cGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTcgcg                                                           >  2:4367872/1‑93 (MQ=255)

                                 ggCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCgg                                                          <  2:1238682/93‑1 (MQ=255)

                                 ggCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCgg                                                          >  1:5447009/1‑93 (MQ=255)

                                  gCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGt                                                         >  2:693134/1‑93 (MQ=255)

                                  gCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGt                                                         <  2:240578/93‑1 (MQ=255)

                                  gCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGt                                                         <  1:1836908/93‑1 (MQ=255)

                                    tCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTa                                                       <  2:5586439/93‑1 (MQ=255)

                                     ccGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTAt                                                      >  2:3219068/1‑93 (MQ=255)

                                     ccGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTAt                                                      <  2:479014/93‑1 (MQ=255)

                                       gTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATcc                                                    <  2:1616640/93‑1 (MQ=255)

                                        tGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATccc                                                   >  2:6355763/1‑93 (MQ=255)

                                         gCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGGTATCCCg                                                  >  1:5542008/1‑93 (MQ=255)

                                          cTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGt                                                 <  1:4801223/93‑1 (MQ=255)

                                           tGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTg                                                >  1:2309309/1‑93 (MQ=255)

                                            gACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTgg                                               >  1:6166131/1‑93 (MQ=255)

                                             aCCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGa                                              >  1:874002/1‑93 (MQ=255)

                                              ccACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGAc                                             >  1:3924660/1‑93 (MQ=255)

                                                aCGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCa                                           <  2:4719811/93‑1 (MQ=255)

                                                aCGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCa                                           <  1:2395124/93‑1 (MQ=255)

                                                 cgccgcCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                          <  2:5280362/93‑1 (MQ=255)

                                                 cgccgcCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                          <  1:495854/93‑1 (MQ=255)

                                                  gccgccGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATg                                         <  1:4718902/93‑1 (MQ=255)

                                                  gccgccGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATg                                         <  2:4888974/93‑1 (MQ=255)

                                                   ccgccgGTTGGGCTGCGTCTTGTGTGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGa                                        <  1:1774435/93‑1 (MQ=255)

                                                   ccgccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGa                                        <  2:1000237/93‑1 (MQ=255)

                                                    cgccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGAt                                       <  1:5847761/93‑1 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      >  2:1569017/1‑93 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      <  2:2280992/93‑1 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      <  1:3159853/93‑1 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      >  2:1077210/1‑93 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      >  1:5209903/1‑93 (MQ=255)

                                                     gccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                      <  1:4239090/93‑1 (MQ=255)

                                                      ccgGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAg                                     <  2:6007037/93‑1 (MQ=255)

                                                         gTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaa                                  >  2:5894660/1‑93 (MQ=255)

                                                         gTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaa                                  <  2:7131730/93‑1 (MQ=255)

                                                          ttGGGCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaaa                                 >  2:1891973/1‑93 (MQ=255)

                                                           tGGGCTGCGTCTTGGATGGTTACGGGTGGGGTGCTCGCGAAATGAAACCCGGCAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaaaa                                >  1:7295253/1‑93 (MQ=255)

                                                            gggCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAt                               <  1:1068356/93‑1 (MQ=255)

                                                             ggCTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAtt                              <  2:1707857/93‑1 (MQ=255)

                                                               cTGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTa                            >  2:4087396/1‑93 (MQ=255)

                                                                tGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                           <  2:809223/93‑1 (MQ=255)

                                                                tGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                           <  2:2953960/93‑1 (MQ=255)

                                                                tGCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                           <  2:2113667/93‑1 (MQ=255)

                                                                 gCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaaa                          <  1:1690801/93‑1 (MQ=255)

                                                                 gCGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaaa                          <  1:7187723/93‑1 (MQ=255)

                                                                  cGTCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaaaa                         <  1:2868931/93‑1 (MQ=255)

                                                                   gTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAAt                        <  2:6510050/93‑1 (MQ=255)

                                                                    tCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATa                       <  2:3351697/93‑1 (MQ=255)

                                                                    tCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATa                       >  1:4439135/1‑93 (MQ=255)

                                                                    tCTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATa                       >  1:5902521/1‑93 (MQ=255)

                                                                     cTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATAc                      <  1:2110536/93‑1 (MQ=255)

                                                                     cTTGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATAc                      <  1:1212025/93‑1 (MQ=255)

                                                                      ttGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACt                     <  1:5082129/93‑1 (MQ=255)

                                                                      ttGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACt                     >  2:6054465/1‑93 (MQ=255)

                                                                       tGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTc                    <  1:5378084/93‑1 (MQ=255)

                                                                       tGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTc                    <  1:304290/93‑1 (MQ=255)

                                                                       tGTATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTc                    <  1:1185598/93‑1 (MQ=255)

                                                                         tATGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGc                  <  2:4679148/93‑1 (MQ=255)

                                                                           tGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGctc                <  1:4763940/93‑1 (MQ=255)

                                                                           tGGTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGctc                <  1:376859/93‑1 (MQ=255)

                                                                            ggTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGctct               >  2:6908600/1‑93 (MQ=255)

                                                                             gTTACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCtctc              <  1:6651275/93‑1 (MQ=255)

                                                                               tACGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCAc            >  2:5120641/1‑93 (MQ=255)

                                                                                aCGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCAcc           >  2:252653/1‑93 (MQ=255)

                                                                                 cGAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCa          <  1:6043463/93‑1 (MQ=255)

                                                                                  gAGTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAg         >  2:6815531/1‑93 (MQ=255)

                                                                                    gTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc       >  1:1568519/1‑93 (MQ=255)

                                                                                    gTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc       <  2:870946/93‑1 (MQ=255)

                                                                                    gTGGGGAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc       >  1:6948184/1‑93 (MQ=255)

                                                                                      ggggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTg     >  1:3439934/1‑93 (MQ=255)

                                                                                       gggAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGc    >  2:3671642/1‑93 (MQ=255)

                                                                                       gggAGCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGc    >  2:3507275/1‑93 (MQ=255)

                                                                                         gACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCaa  <  2:6731973/93‑1 (MQ=255)

CGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAA  >  NC_000913/303978‑304159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`