BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,116,492	C→T	E213K (GAA→AAA)	b2974 ←	putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,116,492	0	C	T	83.8%	776.4 / 94.4	284	E213K (GAA→AAA)	b2974	putative endoglucanase
Reads supporting (aligned to +/- strand): ref base C (18/28); new base T (114/124); total (132/152)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GACGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCG  >  NC_000913/3116400‑3116581

  cGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCg                                                                                         >  2:6054886/1‑93 (MQ=255)

   gggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCgg                                                                                        <  2:4500330/93‑1 (MQ=255)

   gggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCgg                                                                                        >  2:3473910/1‑93 (MQ=255)

    ggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGt                                                                                       >  1:3597118/1‑93 (MQ=255)

     gCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTg                                                                                      >  2:7373415/1‑93 (MQ=255)

          aCCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGa                                                                                 <  2:4792402/93‑1 (MQ=255)

          aCCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGa                                                                                 <  1:3133996/93‑1 (MQ=255)

             ggTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCg                                                                              >  2:1128021/1‑93 (MQ=255)

              gTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGa                                                                             <  1:6893391/93‑1 (MQ=255)

                gAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAtt                                                                           >  1:2762315/1‑93 (MQ=255)

                gAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAtt                                                                           <  2:2175733/93‑1 (MQ=255)

                gAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGAtt                                                                           <  2:5190272/93‑1 (MQ=255)

                 aaGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAttt                                                                          <  2:512862/93‑1 (MQ=255)

                  aGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTg                                                                         >  2:7259928/1‑93 (MQ=255)

                   gTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGc                                                                        <  1:1029016/93‑1 (MQ=255)

                    tATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCt                                                                       >  1:295762/1‑93 (MQ=255)

                     aTGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCtt                                                                      <  1:975202/93‑1 (MQ=255)

                      tGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTta                                                                     >  1:2347095/1‑93 (MQ=255)

                        aGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTtata                                                                   <  1:1833864/93‑1 (MQ=255)

                         gTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATAc                                                                  >  1:4506297/1‑93 (MQ=255)

                          tGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACa                                                                 <  2:3581857/93‑1 (MQ=255)

                          tGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACa                                                                 <  1:696610/93‑1 (MQ=255)

                           ggATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAg                                                                >  2:1928006/1‑93 (MQ=255)

                            nntGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGc                                                               <  2:1390372/91‑1 (MQ=255)

                            gATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGc                                                               <  1:2994398/93‑1 (MQ=255)

                             aTGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCg                                                              >  1:7322862/1‑93 (MQ=255)

                              tGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGa                                                             <  2:2490461/93‑1 (MQ=255)

                                gcgcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGt                                                           <  1:364362/93‑1 (MQ=255)

                                 cgcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTc                                                          <  2:780729/93‑1 (MQ=255)

                                 cgcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTc                                                          <  1:1752641/93‑1 (MQ=255)

                                  gcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCg                                                         >  1:240771/1‑93 (MQ=255)

                                  gcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCg                                                         >  2:5239323/1‑93 (MQ=255)

                                   cTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGa                                                        >  1:4195066/1‑93 (MQ=255)

                                   cTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGa                                                        >  2:1473277/1‑93 (MQ=255)

                                    tttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGaa                                                       >  2:2584237/1‑93 (MQ=255)

                                    tttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGaa                                                       >  1:838454/1‑93 (MQ=255)

                                    tttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGaa                                                       >  2:3912158/1‑93 (MQ=255)

                                    tttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGaa                                                       <  2:1543561/93‑1 (MQ=255)

                                     ttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAg                                                      <  2:5798331/93‑1 (MQ=255)

                                      tATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGc                                                     <  1:647698/93‑1 (MQ=255)

                                      tATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGc                                                     >  2:5604548/1‑93 (MQ=255)

                                         cGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtt                                                  >  2:5456379/1‑93 (MQ=255)

                                         cGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtt                                                  <  1:1554782/93‑1 (MQ=255)

                                           gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTa                                                <  2:1696325/93‑1 (MQ=255)

                                           gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTa                                                >  1:6368691/1‑93 (MQ=255)

                                            tCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTAc                                               <  2:3878858/93‑1 (MQ=255)

                                              gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             <  1:6552675/93‑1 (MQ=255)

                                              gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             >  1:5891187/1‑93 (MQ=255)

                                              gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             >  1:3455353/1‑93 (MQ=255)

                                              gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             >  2:2682738/1‑93 (MQ=255)

                                                agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           <  1:3051294/93‑1 (MQ=255)

                                                agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           <  2:4405856/93‑1 (MQ=255)

                                                agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           >  1:5674020/1‑93 (MQ=255)

                                                agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           >  1:1461268/1‑93 (MQ=255)

                                                  caTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGa                                         <  2:3211828/93‑1 (MQ=255)

                                                  caTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGa                                         >  2:5660224/1‑93 (MQ=255)

                                                  caTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGa                                         >  2:4894910/1‑93 (MQ=255)

                                                    ttGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATc                                       >  1:5345678/1‑93 (MQ=255)

                                                     tGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCa                                      <  2:1617905/93‑1 (MQ=255)

                                                      gTTTTCCACCTCTTCATTTACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCAc                                     >  1:3690588/1‑93 (MQ=255)

                                                      gTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCAc                                     <  2:699009/93‑1 (MQ=255)

                                                       ttttCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCAcc                                    <  1:5010309/93‑1 (MQ=255)

                                                         ttCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCga                                  <  2:5884390/93‑1 (MQ=255)

                                                         ttCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCga                                  <  1:3485298/93‑1 (MQ=255)

                                                          tCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCgag                                 <  2:3085119/93‑1 (MQ=255)

                                                            cACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCgagga                               <  2:7128883/93‑1 (MQ=255)

                                                              ccTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGgana                             >  2:3817037/1‑93 (MQ=255)

                                                              ccTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGgaga                             <  2:2809934/93‑1 (MQ=255)

                                                               ctctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            <  1:4268822/93‑1 (MQ=255)

                                                               ctctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            <  1:1231068/93‑1 (MQ=255)

                                                                  ttCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGt                         >  2:287239/1‑93 (MQ=255)

                                                                  ttCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGt                         <  2:1358270/93‑1 (MQ=255)

                                                                  ttCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGt                         <  2:4171569/93‑1 (MQ=255)

                                                                  ttCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGt                         <  1:7038185/93‑1 (MQ=255)

                                                                   tCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTc                        <  2:1196621/93‑1 (MQ=255)

                                                                    cATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCa                       <  2:6524109/93‑1 (MQ=255)

                                                                    cATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCa                       >  2:2076499/1‑93 (MQ=255)

                                                                     aTTGACCAGCTTTTTGATCTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  1:671419/1‑93 (MQ=255)

                                                                     aTTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  2:3301558/1‑93 (MQ=255)

                                                                     aTTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  1:6066572/1‑93 (MQ=255)

                                                                     aTTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  1:740693/1‑93 (MQ=255)

                                                                      ttGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGa                     >  2:4073825/1‑93 (MQ=255)

                                                                         aCCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACaa                  <  1:3207834/93‑1 (MQ=255)

                                                                          ccAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACaaa                 >  1:2207706/1‑93 (MQ=255)

                                                                           cAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAAc                <  2:4636092/93‑1 (MQ=255)

                                                                             gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              <  1:4816243/93‑1 (MQ=255)

                                                                             gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              <  1:5757829/93‑1 (MQ=255)

                                                                             gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              >  1:1964644/1‑93 (MQ=255)

                                                                               tttttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGt            <  1:4012622/93‑1 (MQ=255)

                                                                                ttttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGtt           >  1:5537358/1‑93 (MQ=255)

                                                                                 tttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTc          >  1:1267770/1‑93 (MQ=255)

                                                                                  ttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCt         <  2:6471456/93‑1 (MQ=255)

                                                                                  ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCt         >  1:4695747/1‑93 (MQ=255)

                                                                                    gAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGt       >  1:719043/1‑93 (MQ=255)

                                                                                    gAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGt       >  2:6222775/1‑93 (MQ=255)

                                                                                     aaCTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTa      <  1:5285344/93‑1 (MQ=255)

                                                                                      aCTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTAt     >  1:4450294/1‑93 (MQ=255)

                                                                                       ctctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTAtt    <  1:3304921/93‑1 (MQ=255)

                                                                                       ctctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTAtt    <  1:4777074/93‑1 (MQ=255)

                                                                                         ctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCg  <  2:4818443/93‑1 (MQ=255)

GACGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCG  >  NC_000913/3116400‑3116581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`