BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	307,659	T→C	T300A (ACC→GCC)	yagX ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	307,659	0	T	C	83.5%	513.8 / 77.4	257	T300A (ACC→GCC)	yagX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (25/17); new base C (108/104); total (133/124)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 259 total aligned reads displayed.

CACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCC  >  NC_000913/307567‑307751

  caTCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCga                                                                                            <  1:2040020/93‑1 (MQ=255)

  caTCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCga                                                                                            <  1:6699375/93‑1 (MQ=255)

    tCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCgaga                                                                                          >  2:890451/1‑93 (MQ=255)

    tCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCgaga                                                                                          >  1:2326405/1‑93 (MQ=255)

    tCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCgaga                                                                                          >  1:4195486/1‑93 (MQ=255)

     cGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGAt                                                                                         <  2:1505017/93‑1 (MQ=255)

       tAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGt                                                                                       >  1:1049882/1‑93 (MQ=255)

       tAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGt                                                                                       >  1:6487682/1‑93 (MQ=255)

        aaaTCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTa                                                                                      <  2:4532774/93‑1 (MQ=255)

         aaTCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑AGGCGAGATGTAc                                                                                     >  1:4456237/1‑93 (MQ=255)

          aTCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACt                                                                                    <  2:7420283/93‑1 (MQ=255)

          aTCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACt                                                                                    >  1:5339343/1‑93 (MQ=255)

          aTCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACt                                                                                    <  1:475302/93‑1 (MQ=255)

           tCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACtt                                                                                   <  2:4826403/93‑1 (MQ=255)

           tCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACtt                                                                                   >  1:930359/1‑93 (MQ=255)

            cccGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTc                                                                                  <  1:6259500/93‑1 (MQ=255)

            cccGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTc                                                                                  <  2:490604/93‑1 (MQ=255)

                   ggTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTcgcccgcc                                                                           >  2:373883/1‑93 (MQ=255)

                    gTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCccgccg                                                                          >  2:5117043/1‑93 (MQ=255)

                      aGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCccgccgcc                                                                        <  2:2918027/93‑1 (MQ=255)

                      aGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCccgccgcc                                                                        <  2:4439033/93‑1 (MQ=255)

                       gACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCccgccgccg                                                                       <  2:4150520/93‑1 (MQ=255)

                        aCCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCgg                                                                      <  2:3656151/93‑1 (MQ=255)

                        aCCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCgg                                                                      <  2:223156/93‑1 (MQ=255)

                        aCCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCgg                                                                      <  2:5668096/93‑1 (MQ=255)

                          cccGGGTATCCACTTCATGATTACCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTa                                                                    >  1:6671780/1‑93 (MQ=255)

                           ccGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAG‑GGGTGAGATGTACTTCGCCCGCCGCCGGTaa                                                                   <  1:3226685/93‑1 (MQ=255)

                            cGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTaaa                                                                  >  2:560297/1‑93 (MQ=255)

                            cGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTaaa                                                                  >  1:4998147/1‑93 (MQ=255)

                             gggTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTaaaa                                                                 >  1:3173030/1‑93 (MQ=255)

                              ggTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTaaaaa                                                                >  1:5734276/1‑93 (MQ=255)

                               gTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAG‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAg                                                               <  1:360097/93‑1 (MQ=255)

                               gTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAg                                                               >  1:6819111/1‑93 (MQ=255)

                                tATCCACTTCATGATTGCCCATAGTGAATTTCTGACCGCTTAGTAGCCGCCCATCAC‑GGGGGAGATGTACTTCGCCCGCCGCCGGTAAAAAgg                                                              <  2:3093077/93‑1 (MQ=255)

                                tATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAgg                                                              <  1:301241/93‑1 (MQ=255)

                                tATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAgg                                                              <  2:4234981/93‑1 (MQ=255)

                                 aTCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGc                                                             >  1:2466675/1‑93 (MQ=255)

                                  tCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCg                                                            >  1:3073753/1‑93 (MQ=255)

                                  tCCACTTCATGATTGCCCATAGTGAAGATCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCg                                                            >  2:6431182/1‑93 (MQ=255)

                                    cACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGAt                                                          >  1:4078148/1‑93 (MQ=255)

                                     aCTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATc                                                         <  1:850161/93‑1 (MQ=255)

                                     aCTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATc                                                         >  2:2928746/1‑93 (MQ=255)

                                     aCTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATc                                                         <  1:1173599/93‑1 (MQ=255)

                                     aCTGCATGATTGCCCATGGTGAAGTTCTGAACGCTTAGTGGCCGCCCATCAC‑GGGTGAAATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATc                                                         <  1:5214075/93‑1 (MQ=255)

                                      ctccATGTTTGCCCATAGTGAATTTCTGAACCCTTAGTCGCCCCCCTTCCC‑GGGCGAGATGTACTCCGCCCGCCGCCGGTAAAAAGGCGATCa                                                        <  1:2927168/90‑1 (MQ=255)

                                      cTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCa                                                        >  2:6610566/1‑93 (MQ=255)

                                      cTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCa                                                        >  2:3207849/1‑93 (MQ=255)

                                       ttCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCAc                                                       <  1:7183611/93‑1 (MQ=255)

                                        tCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACt                                                      <  1:378437/93‑1 (MQ=255)

                                          aggATTGCCAATAGTGAATTTCTGACCGTTTAGTACCCCCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTgg                                                    <  1:4289611/91‑1 (MQ=255)

                                          aTGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTgg                                                    >  2:2263901/1‑93 (MQ=255)

                                           tGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGc                                                   >  2:1811004/1‑93 (MQ=255)

                                            gATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCg                                                  >  1:5866614/1‑93 (MQ=255)

                                             aTTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGt                                                 <  1:2555758/93‑1 (MQ=255)

                                               tGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTgg                                               >  2:6562356/1‑93 (MQ=255)

                                                gCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCCC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGc                                              <  1:2135325/93‑1 (MQ=255)

                                                gCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGc                                              <  2:5433253/93‑1 (MQ=255)

                                                  ccATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTg                                            >  1:2419399/1‑93 (MQ=255)

                                                    aTAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGAc                                          >  1:7382391/1‑93 (MQ=255)

                                                    aTAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGAc                                          <  2:6198862/93‑1 (MQ=255)

                                                     tAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACt                                         >  2:5324397/1‑93 (MQ=255)

                                                       gTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTgg                                       >  1:5231145/1‑93 (MQ=255)

                                                          aaGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                    >  2:6721758/1‑93 (MQ=255)

                                                          aaGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                    >  2:7067565/1‑93 (MQ=255)

                                                          aaGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                    >  2:2446776/1‑93 (MQ=255)

                                                           atttCTAAACGTTTAGTAGCCCCCCATCAC‑GGGCGAGATGTATTCCCCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctgc                                   <  1:6277336/91‑1 (MQ=255)

                                                           aGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGTTGTACTTGCCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctgc                                   <  1:4368763/93‑1 (MQ=255)

                                                            gTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGgctgct                                  >  2:3364969/1‑93 (MQ=255)

                                                             ttCTGAACGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGctgctg                                 <  2:6933210/93‑1 (MQ=255)

                                                                 gAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGa                             >  2:540288/1‑93 (MQ=255)

                                                                    cGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGa                          >  2:147317/1‑93 (MQ=255)

                                                                    cGCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGa                          >  1:4403362/1‑93 (MQ=255)

                                                                    cGCTTAGTACCCGCCCATCCC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGa                          <  1:6327953/93‑1 (MQ=255)

                                                                     gCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAt                         >  1:3697963/1‑93 (MQ=255)

                                                                     gCTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGAGTGGCTGACTGGCTGCTGTCGAAGAt                         <  2:2035328/93‑1 (MQ=255)

                                                                     gCTTAGTAGCCCCCCATCCC‑GGGCGAGTTGTACTTCCCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAt                         <  1:6808055/93‑1 (MQ=255)

                                                                      cTTAGTAGCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtg                        >  2:2726773/1‑93 (MQ=255)

                                                                      cTTAGTAGCCCCCCCACACGGGGGGAATTGTCCTCCCCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAt                         <  1:6755963/93‑1 (MQ=255)

                                                                       ttAGTAGCCGCCCATCAG‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtgg                       <  1:740021/93‑1 (MQ=255)

                                                                        tagtagCCCCCCATCAG‑GGGCGAGATGTCCTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtggt                      <  1:1544862/93‑1 (MQ=255)

                                                                         agtagCCGCCCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtggtg                     <  2:2517014/93‑1 (MQ=255)

                                                                             gccgccCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGc                 >  2:6216157/1‑93 (MQ=255)

                                                                             gccgccCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGc                 <  2:3546591/93‑1 (MQ=255)

                                                                              ccgccCATCAG‑GGGCGAGATGTCCTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCt                <  1:5485915/93‑1 (MQ=255)

                                                                              ccgccCACCAG‑GGGCGAGATGTCCTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCt                <  1:384293/93‑1 (MQ=255)

                                                                                gccCATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTgg              >  2:1193564/1‑93 (MQ=255)

                                                                                 cccATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGc             >  1:3314773/1‑93 (MQ=255)

                                                                                 cccATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGc             >  2:1299730/1‑93 (MQ=255)

                                                                                  ccATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGcc            <  2:7105619/93‑1 (MQ=255)

                                                                                   cATCAG‑GGGCGATATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCt           <  1:5989862/93‑1 (MQ=255)

                                                                                   cATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCt           >  2:6678867/1‑93 (MQ=255)

                                                                                   cATCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCt           <  2:4722849/93‑1 (MQ=255)

                                                                                    aTCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTg          <  2:7478828/93‑1 (MQ=255)

                                                                                     tCAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTgg         >  2:1649224/1‑93 (MQ=255)

                                                                                      cAC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGt        >  2:5967150/1‑93 (MQ=255)

                                                                                       aC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGtt       >  2:4656054/1‑93 (MQ=255)

                                                                                       aC‑GGGCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCCGGCCTGGtt       >  1:1835161/1‑93 (MQ=255)

                                                                                          gggCGAGTTGTACTCCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTc     <  1:2778349/93‑1 (MQ=255)

                                                                                          gggCGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTc     >  2:4029459/1‑93 (MQ=255)

                                                                                            gTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTccc   <  2:1044549/93‑1 (MQ=255)

CACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCAC‑GGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCC  >  NC_000913/307567‑307751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`