Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,132,556 T→C R137R (CGT→CGC)  yghT → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,132,5560TC81.9% 639.8 / 154.2 331R137R (CGT→CGC) yghThypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base C (145/126);  total (177/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

Only 100 of 340 total aligned reads displayed.

GGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCT  >  NC_000913/3132464‑3132648
                                                                                            |                                                                                            
 ggTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGtt                                                                                             <  2:2850370/93‑1 (MQ=255)
  gTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGctt                                                                                            <  2:2404687/93‑1 (MQ=255)
    tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTg                                                                                          <  2:6056386/93‑1 (MQ=255)
         gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCa                                                                                     <  2:1266412/93‑1 (MQ=255)
         gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCa                                                                                     <  2:1354875/93‑1 (MQ=255)
          ttCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCAt                                                                                    <  1:4725025/93‑1 (MQ=255)
            cTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCAtgg                                                                                  >  2:5816222/1‑93 (MQ=255)
             tAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTTCGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCAtggc                                                                                 >  1:7514611/1‑93 (MQ=255)
             tAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCAtggc                                                                                 >  2:2660377/1‑93 (MQ=255)
             tAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCAtggc                                                                                 <  2:7162541/93‑1 (MQ=255)
               accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCAtggctg                                                                               >  1:5067433/1‑93 (MQ=255)
               accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCAtggctg                                                                               <  2:298551/93‑1 (MQ=255)
                ccgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCAtggctgg                                                                              <  1:4614667/93‑1 (MQ=255)
                ccgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCAtggctgg                                                                              >  1:5594466/1‑93 (MQ=255)
                   accgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCaa                                                                           >  2:3559478/1‑93 (MQ=255)
                     cgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGc                                                                         >  2:2135403/1‑93 (MQ=255)
                      gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGcc                                                                        >  1:4062840/1‑93 (MQ=255)
                      gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGcc                                                                        >  1:625284/1‑93 (MQ=255)
                      gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGcc                                                                        >  1:5208885/1‑93 (MQ=255)
                      gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGcc                                                                        <  2:4047192/93‑1 (MQ=255)
                       ttATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCa                                                                       >  1:1280152/1‑93 (MQ=255)
                       ttATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCa                                                                       >  2:5411518/1‑93 (MQ=255)
                       ttATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCa                                                                       >  2:3169806/1‑93 (MQ=255)
                         aTCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAgg                                                                     >  1:6985371/1‑93 (MQ=255)
                          tCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGa                                                                    >  1:80675/1‑93 (MQ=255)
                          tCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGa                                                                    >  2:3258007/1‑93 (MQ=255)
                           ccTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGaa                                                                   <  2:779247/93‑1 (MQ=255)
                           ccTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGaa                                                                   <  1:3238765/93‑1 (MQ=255)
                            cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAAc                                                                  <  1:1945658/93‑1 (MQ=255)
                            cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAAc                                                                  >  1:5528494/1‑93 (MQ=255)
                             tCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACg                                                                 >  1:1399358/1‑93 (MQ=255)
                               aGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTa                                                               <  2:6888135/93‑1 (MQ=255)
                                ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                              >  1:6582767/1‑93 (MQ=255)
                                ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                              <  2:2224166/93‑1 (MQ=255)
                                ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                              <  1:5289446/93‑1 (MQ=255)
                                ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                              <  2:6323801/93‑1 (MQ=255)
                                 gACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTaaa                                                             <  1:840545/93‑1 (MQ=255)
                                 gACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTaaa                                                             >  2:5005471/1‑93 (MQ=255)
                                  aCCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAg                                                            >  2:3896906/1‑93 (MQ=255)
                                  aCCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAg                                                            >  2:2591923/1‑93 (MQ=255)
                                      aaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGAAAGCCAGGAACGTAAAGCGtt                                                        >  1:6479184/1‑93 (MQ=255)
                                        ttCCTGGCGCTTACGATGGTCGGGTGTCCCACCCAAACGTTGAAGGCGGTCCCTTTGTCTAATGGCTGGCAAGCCAGGAACGTAAAGCGTTTc                                                      <  1:6157189/93‑1 (MQ=255)
                                        ttCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTc                                                      >  2:7249178/1‑93 (MQ=255)
                                        ttCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGCACGTAAAGCGTTTc                                                      <  2:6453356/93‑1 (MQ=255)
                                        ttCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTc                                                      >  2:4612993/1‑93 (MQ=255)
                                         tCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCa                                                     <  1:1867473/93‑1 (MQ=255)
                                          ccTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCAc                                                    <  1:4210461/93‑1 (MQ=255)
                                           cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                                   >  2:3312226/1‑93 (MQ=255)
                                           cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                                   >  1:4438897/1‑93 (MQ=255)
                                           cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                                   <  2:7524209/93‑1 (MQ=255)
                                           cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                                   <  1:741561/93‑1 (MQ=255)
                                            tGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                                  >  2:5098778/1‑93 (MQ=255)
                                            tGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                                  <  2:4277006/93‑1 (MQ=255)
                                              gcgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGa                                                >  2:6506593/1‑93 (MQ=255)
                                               cgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                               <  1:2313826/93‑1 (MQ=255)
                                               cgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                               <  1:2215087/93‑1 (MQ=255)
                                               cgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                               <  1:4691005/93‑1 (MQ=255)
                                                  ttACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATggg                                            >  1:2858671/1‑92 (MQ=255)
                                                   tACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                           <  1:6356951/93‑1 (MQ=255)
                                                    aCGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGa                                          <  1:7243737/93‑1 (MQ=255)
                                                      gATGGTACGGTGTTCCCACCTAACGTTGAAGGGGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                        >  2:2311668/1‑93 (MQ=255)
                                                      gATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                        <  2:2779370/93‑1 (MQ=255)
                                                      gATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                        >  2:2031391/1‑93 (MQ=255)
                                                      gATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                        >  1:7055949/1‑93 (MQ=255)
                                                       aTGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGcc                                       >  2:6671715/1‑93 (MQ=255)
                                                       aTGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGcc                                       <  1:5233771/93‑1 (MQ=255)
                                                       aTGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGcc                                       <  2:6573039/93‑1 (MQ=255)
                                                         ggTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCAt                                     >  2:4346503/1‑93 (MQ=255)
                                                           tACGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATaa                                   >  2:7373878/1‑93 (MQ=255)
                                                            aCGGTGTTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAg                                  <  2:5255588/93‑1 (MQ=255)
                                                               gtgtTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCt                               >  2:5684903/1‑93 (MQ=255)
                                                               gtgtTCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCt                               >  2:5900090/1‑93 (MQ=255)
                                                                  ttCCCGCCTAACGTTGAAGGGGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGAt                            <  2:7084992/93‑1 (MQ=255)
                                                                  ttCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGAt                            <  2:4194148/93‑1 (MQ=255)
                                                                   tCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATc                           <  1:1111451/93‑1 (MQ=255)
                                                                   tCCCACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATc                           >  1:4578432/1‑93 (MQ=255)
                                                                    cccACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAACCATAAGCCTGATCt                          >  1:591369/1‑93 (MQ=255)
                                                                      cACTTAACGTTGAAGGCGGTGGCTTTGTCTCATGGCGGGAAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTgg                        <  1:1476753/93‑1 (MQ=255)
                                                                      cACCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTgg                        >  1:4795373/1‑93 (MQ=255)
                                                                       cccTACCGTTGAGGGTGGTCGTTTTGTCTCATGGCGGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGt                       <  1:2638571/92‑1 (MQ=255)
                                                                       aCCTAAGGTTGAAGGTGGTTTTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGt                       <  1:4269878/93‑1 (MQ=255)
                                                                       aCCTAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGGGAGCCATAAGCCCGATCTGGt                       >  1:3633043/1‑93 (MQ=255)
                                                                        ccaaaCGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGAAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtc                      <  1:3873387/90‑1 (MQ=255)
                                                                          tAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcat                    >  1:3946180/1‑93 (MQ=255)
                                                                          tAACGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcat                    <  2:1482422/93‑1 (MQ=255)
                                                                          tAACGTTGAAGGCGGCCTCTTTGTCTCAGGGCGGGAAAGCCAGGAACGTAAAGCGTTTCACTGGAGGGCGAGCCATAAGCCTGATCTGGtcat                    <  1:3562012/93‑1 (MQ=255)
                                                                            aCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcatca                  <  2:1906688/93‑1 (MQ=255)
                                                                            aCGTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcatca                  >  1:1022906/1‑93 (MQ=255)
                                                                             cGTTGAGGGTGGTCTTTTTGTCTCATGGCGGGAAAGCCAGGAACGTAAAGCGTTTCACTGGAGGGGGAGCCATAAGCCTGATCTGGTCATCaa                 <  1:5759107/93‑1 (MQ=255)
                                                                              gTTGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCaaa                >  2:1000551/1‑93 (MQ=255)
                                                                               ttGAAGGCGTCCGCTTTGTCTCAGGGCTGGCAAGCCAGGAACAAAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAAc               <  1:6722029/93‑1 (MQ=255)
                                                                               ttGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAAc               <  2:1787531/93‑1 (MQ=255)
                                                                               tggAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAAc               <  1:5449073/91‑1 (MQ=255)
                                                                                tGAAGGCGGTCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACt              <  2:4862198/93‑1 (MQ=255)
                                                                                   aGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCaa           >  1:4245488/1‑93 (MQ=255)
                                                                                    gggggtCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAAt          <  1:1786184/90‑1 (MQ=255)
                                                                                     gcggtCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATg         >  1:244567/3‑93 (MQ=255)
                                                                                       ggtCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGtt       >  1:4950670/1‑93 (MQ=255)
                                                                                        gtCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTg      >  1:3198985/1‑93 (MQ=255)
                                                                                        gtCGCTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTg      <  2:3501015/93‑1 (MQ=255)
                                                                                            |                                                                                            
GGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCT  >  NC_000913/3132464‑3132648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑