BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,775	A→G	V503A (GTG→GCG)	glnE ←	GlnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,775	0	A	G	83.3%	513.9 / 91.4	233	V503A (GTG→GCG)	glnE	GlnE
Reads supporting (aligned to +/- strand): ref base A (17/22); new base G (82/112); total (99/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTA  >  NC_000913/3195684‑3195867

    aGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGa                                                                                         <  1:2490741/93‑1 (MQ=255)

    aGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGa                                                                                         <  2:2579368/93‑1 (MQ=255)

       aaGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGt                                                                                      <  1:3468978/93‑1 (MQ=255)

        aGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTc                                                                                     >  2:4983953/1‑93 (MQ=255)

        aGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTc                                                                                     >  1:2957443/1‑93 (MQ=255)

         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    <  2:7470584/93‑1 (MQ=255)

         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    >  2:1903308/1‑93 (MQ=255)

         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    <  1:4155562/93‑1 (MQ=255)

          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCt                                                                                   <  1:7281043/93‑1 (MQ=255)

          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCt                                                                                   >  1:1324815/1‑93 (MQ=255)

          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCt                                                                                   >  1:6895184/1‑93 (MQ=255)

            ggTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcg                                                                                 <  2:5592243/93‑1 (MQ=255)

             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  1:6140090/93‑1 (MQ=255)

             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  1:852430/93‑1 (MQ=255)

             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  2:6041264/93‑1 (MQ=255)

             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  2:7205386/93‑1 (MQ=255)

             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                >  1:604913/1‑93 (MQ=255)

              tAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgcg                                                                               >  2:3550542/1‑93 (MQ=255)

              tAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTcgcg                                                                               >  1:4575827/1‑93 (MQ=255)

                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            >  1:6668868/1‑93 (MQ=255)

                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  2:3878775/93‑1 (MQ=255)

                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  1:3192404/93‑1 (MQ=255)

                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  2:7247535/93‑1 (MQ=255)

                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         >  2:2568894/1‑93 (MQ=255)

                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:795123/93‑1 (MQ=255)

                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  2:1189973/93‑1 (MQ=255)

                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:2532916/93‑1 (MQ=255)

                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:2075886/93‑1 (MQ=255)

                      gACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGTGGCCCGATg                                                                       >  2:3496777/1‑93 (MQ=255)

                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  2:1255030/93‑1 (MQ=255)

                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  1:1231998/93‑1 (MQ=255)

                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  2:953197/93‑1 (MQ=255)

                        cAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGt                                                                     <  1:1262417/93‑1 (MQ=255)

                        cAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGt                                                                     <  1:92300/93‑1 (MQ=255)

                          gCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTgc                                                                   <  1:2306015/93‑1 (MQ=255)

                            gTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTgcgc                                                                 >  2:144070/1‑93 (MQ=255)

                             tAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCt                                                                >  1:5615069/1‑93 (MQ=255)

                             tAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCt                                                                <  1:2142963/93‑1 (MQ=255)

                              aaCGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCtt                                                               <  2:658545/93‑1 (MQ=255)

                              aaCGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCtt                                                               >  2:1161460/1‑93 (MQ=255)

                                 ggCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATc                                                            <  1:135744/93‑1 (MQ=255)

                                 ggCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATc                                                            <  1:4731209/93‑1 (MQ=255)

                                     gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGc                                                        >  1:2217900/1‑93 (MQ=255)

                                     gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGc                                                        <  1:3337721/93‑1 (MQ=255)

                                        tCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGctct                                                     >  1:4049269/1‑93 (MQ=255)

                                        tCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGctct                                                     >  1:1882867/1‑93 (MQ=255)

                                          ttCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCttt                                                   >  1:1518836/1‑93 (MQ=255)

                                          ttCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCttt                                                   <  1:3649916/93‑1 (MQ=255)

                                           tCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTg                                                  <  1:4810494/93‑1 (MQ=255)

                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                <  1:4987386/93‑1 (MQ=255)

                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                >  1:7495195/1‑93 (MQ=255)

                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                >  1:6942129/1‑93 (MQ=255)

                                               gcgcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGa                                              >  1:5661516/1‑93 (MQ=255)

                                                 gcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGTCCCGATGGTGCGCTTATCCAGCTCTTTGCGGaaa                                            <  2:2702074/93‑1 (MQ=255)

                                                 gcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGaaa                                            <  1:2855007/93‑1 (MQ=255)

                                                   acaGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATc                                          <  2:1346640/93‑1 (MQ=255)

                                                   acaGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATc                                          <  2:2973040/93‑1 (MQ=255)

                                                    caGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCg                                         >  1:430614/1‑93 (MQ=255)

                                                      gACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGc                                       >  1:679548/1‑93 (MQ=255)

                                                      gACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGc                                       <  2:3300633/93‑1 (MQ=255)

                                                        cATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCaa                                     >  1:4116180/1‑93 (MQ=255)

                                                        cATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCaa                                     <  2:5060038/93‑1 (MQ=255)

                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    <  2:671860/93‑1 (MQ=255)

                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    <  2:2885072/93‑1 (MQ=255)

                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    >  2:3163322/1‑93 (MQ=255)

                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   >  1:1576782/1‑93 (MQ=255)

                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   <  1:244516/93‑1 (MQ=255)

                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   <  1:2682036/93‑1 (MQ=255)

                                                           cACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCa                                  >  1:1132696/1‑93 (MQ=255)

                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAg                                 >  1:5166615/1‑93 (MQ=255)

                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAg                                 >  1:6527562/1‑93 (MQ=255)

                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCAGCAATCAg                                 <  1:3767070/93‑1 (MQ=255)

                                                             cTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGc                                <  2:6197952/93‑1 (MQ=255)

                                                             cTTAGCAGATGCGGCATCAGATGGACGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGc                                <  2:4788503/93‑1 (MQ=255)

                                                               tAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGt                              >  1:3034164/1‑93 (MQ=255)

                                                                aGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGtt                             <  2:2069750/93‑1 (MQ=255)

                                                                 gCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTa                            <  2:5680359/93‑1 (MQ=255)

                                                                  cAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAg                           <  1:7177642/93‑1 (MQ=255)

                                                                  cAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAg                           <  2:5312072/93‑1 (MQ=255)

                                                                     aTGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGcgc                        <  2:5957638/93‑3 (MQ=255)

                                                                      tGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGcc                       <  1:3821651/93‑1 (MQ=255)

                                                                         ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                    <  2:4405038/93‑1 (MQ=255)

                                                                         ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                    >  2:4029894/1‑93 (MQ=255)

                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  2:2599592/93‑1 (MQ=255)

                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  1:5292412/93‑1 (MQ=255)

                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  2:163541/93‑1 (MQ=255)

                                                                           cATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGttt                  <  1:6407472/93‑1 (MQ=255)

                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                >  2:2785734/1‑93 (MQ=255)

                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                <  2:4612022/93‑1 (MQ=255)

                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                >  2:4190321/1‑93 (MQ=255)

                                                                              cAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCg               <  2:5473746/93‑1 (MQ=255)

                                                                               aGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGa              >  2:1658584/1‑93 (MQ=255)

                                                                                 aTGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATGAGCGTTAGCGCCTGTTTGCGatc            >  2:6971093/1‑93 (MQ=255)

                                                                                  tGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatca           <  2:2335441/93‑1 (MQ=255)

                                                                                   ggTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcat          <  2:7269726/93‑1 (MQ=255)

                                                                                    gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc         <  2:1585069/93‑1 (MQ=255)

                                                                                    gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc         >  1:3137161/1‑93 (MQ=255)

                                                                                       gAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTc      <  2:2845184/93‑1 (MQ=255)

                                                                                        aGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCg     >  1:6247971/1‑93 (MQ=255)

                                                                                           aCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCCGTTTGCGATCATCCTCGCTa  >  1:5400581/1‑93 (MQ=255)

CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTA  >  NC_000913/3195684‑3195867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`