BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,844	A→G	V480A (GTG→GCG)	glnE ←	GlnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,844	0	A	G	80.2%	464.0 / 114.7	232	V480A (GTG→GCG)	glnE	GlnE
Reads supporting (aligned to +/- strand): ref base A (22/24); new base G (114/72); total (136/96)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

ATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGT  >  NC_000913/3195753‑3195933

 tGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGcc                                                                                         >  1:7330493/1‑93 (MQ=255)

 tGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGcc                                                                                         <  1:3821651/93‑1 (MQ=255)

  gCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCt                                                                                        <  2:982477/93‑1 (MQ=255)

    ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                                                                                      >  2:4029894/1‑93 (MQ=255)

    ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                                                                                      <  2:4405038/93‑1 (MQ=255)

     gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                                                                                     <  2:163541/93‑1 (MQ=255)

     gCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGtt                                                                                     >  2:4149407/1‑93 (MQ=255)

      cATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGttt                                                                                    <  1:6407472/93‑1 (MQ=255)

       aTCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTg                                                                                   >  1:5698665/1‑93 (MQ=255)

        tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                                                                                  >  2:1396006/1‑93 (MQ=255)

         cAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCg                                                                                 <  1:846634/93‑1 (MQ=255)

         cAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCg                                                                                 >  1:6772252/1‑93 (MQ=255)

             tGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatca                                                                             <  2:4924995/93‑1 (MQ=255)

              ggTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcat                                                                            <  2:7269726/93‑1 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatgatc                                                                           <  1:6973482/93‑1 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           >  1:1172789/1‑93 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           <  2:2792086/93‑1 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           >  1:3137161/1‑93 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           <  2:6923477/93‑1 (MQ=255)

               gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           <  2:7169533/93‑1 (MQ=255)

                  gAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTc                                                                        <  2:2845184/93‑1 (MQ=255)

                  gAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTc                                                                        <  2:2912354/93‑1 (MQ=255)

                    gcgccTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGc                                                                      >  1:205922/4‑93 (MQ=255)

                      gccTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTa                                                                    >  2:3957586/2‑93 (MQ=255)

                      aCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCCGTTTGCGATCATCCTCGCTa                                                                    >  1:5400581/1‑93 (MQ=255)

                        cTGCCGCCCTCCCGCCCGGTTGTTGCGCTAATCCGGCTTTTTGC‑GAAAACGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGa                                                                 <  1:2626819/93‑1 (MQ=255)

                        cTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAg                                                                  >  1:6234312/1‑93 (MQ=255)

                        cTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAg                                                                  >  2:320360/1‑93 (MQ=255)

                         tGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTATGa                                                                 >  1:6165716/1‑93 (MQ=255)

                         tGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGa                                                                 <  2:5019542/93‑1 (MQ=255)

                          gACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGAt                                                                >  2:3477688/1‑93 (MQ=255)

                          gACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGAt                                                                >  1:5138344/1‑93 (MQ=255)

                          gACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGAt                                                                >  2:79875/1‑93 (MQ=255)

                           aCGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATg                                                               <  1:5390004/93‑1 (MQ=255)

                           aCGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATg                                                               >  2:6269507/1‑93 (MQ=255)

                           aCGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATg                                                               <  2:5838395/93‑1 (MQ=255)

                            cGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgc                                                              <  2:1286914/93‑1 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTCTCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            <  2:4783454/93‑1 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  2:4370711/1‑93 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  1:6375707/1‑93 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  1:6948059/1‑93 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  1:3404346/1‑93 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  2:5747744/1‑93 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            <  1:1764894/93‑1 (MQ=255)

                              tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            <  1:4505446/93‑1 (MQ=255)

                               ccTCGCGTCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGcc                                                           <  1:609041/93‑1 (MQ=255)

                               ccTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGcc                                                           <  1:4325613/93‑1 (MQ=255)

                                cTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCa                                                          >  2:224843/1‑93 (MQ=255)

                                cTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCa                                                          <  1:1047558/93‑1 (MQ=255)

                                cTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCa                                                          <  2:2541371/93‑1 (MQ=255)

                                 tCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAg                                                         >  2:4554294/1‑93 (MQ=255)

                                 tCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGCCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAg                                                         >  1:2509166/1‑93 (MQ=255)

                                   gcgGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCa                                                       <  1:5487531/93‑1 (MQ=255)

                                   gcgGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCa                                                       <  2:2954891/93‑1 (MQ=255)

                                      gCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTg                                                    <  2:4489930/93‑1 (MQ=255)

                                       cccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTgg                                                   <  1:4128013/93‑1 (MQ=255)

                                        ccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGc                                                  >  1:506537/1‑93 (MQ=255)

                                        ccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGc                                                  >  1:1032203/1‑93 (MQ=255)

                                         cGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCg                                                 >  2:1679097/1‑93 (MQ=255)

                                         cGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGACg                                                 >  2:3868114/1‑93 (MQ=255)

                                          gATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGt                                                >  1:958753/1‑93 (MQ=255)

                                          gATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGt                                                <  1:5867087/93‑1 (MQ=255)

                                           aTGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTa                                               >  2:2411724/1‑93 (MQ=255)

                                            tGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAg                                              <  2:558260/93‑1 (MQ=255)

                                             ggTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgt                                             <  1:4919340/93‑1 (MQ=255)

                                              gTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCATGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgtg                                            <  2:6160635/93‑1 (MQ=255)

                                                gcgcTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTc                                          >  1:3298339/1‑93 (MQ=255)

                                                  gcTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCAt                                        <  2:1567742/93‑1 (MQ=255)

                                                   cTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATc                                       <  1:5553662/93‑1 (MQ=255)

                                                   cTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATc                                       <  2:2755887/93‑1 (MQ=255)

                                                    ttATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCt                                      >  1:2250162/1‑93 (MQ=255)

                                                    ttATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCt                                      >  2:2615476/1‑93 (MQ=255)

                                                      aTCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATCCGCCAGCACTGGCGTAGTGTCATCTTc                                    <  2:6420621/93‑1 (MQ=255)

                                                         cAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTg                                 >  2:1024098/1‑93 (MQ=255)

                                                          aGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGc                                >  1:7237170/1‑93 (MQ=255)

                                                              ctTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACg                            >  2:932509/1‑93 (MQ=255)

                                                               tttGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGc                           >  2:6596826/1‑93 (MQ=255)

                                                                 tGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCAt                         <  1:2994606/93‑1 (MQ=255)

                                                                  gCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATc                        >  1:2763260/1‑93 (MQ=255)

                                                                   cGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATcc                       <  2:5351805/93‑1 (MQ=255)

                                                                    ggAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCt                      <  1:7064107/93‑1 (MQ=255)

                                                                     gAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTg                     >  1:2364974/1‑93 (MQ=255)

                                                                          cGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCcaca                >  2:599484/1‑93 (MQ=255)

                                                                           ggCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAg               >  2:1412838/1‑93 (MQ=255)

                                                                            gCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGc              >  2:1772153/1‑93 (MQ=255)

                                                                              aaTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTc            >  1:4783655/1‑93 (MQ=255)

                                                                               aTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCa           >  2:5022026/1‑93 (MQ=255)

                                                                               aTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCa           >  2:1981866/1‑93 (MQ=255)

                                                                               aTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCa           <  1:144070/93‑1 (MQ=255)

                                                                                tCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCAc          >  2:4090003/1‑93 (MQ=255)

                                                                                tCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCAc          >  2:3794397/1‑93 (MQ=255)

                                                                                  aGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGc        >  1:4179930/1‑93 (MQ=255)

                                                                                    cGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCa      <  2:2189146/93‑1 (MQ=255)

                                                                                     gTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCAc     >  1:3720217/1‑93 (MQ=255)

                                                                                     gTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCAc     >  2:6514135/1‑93 (MQ=255)

                                                                                      ttAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACt    >  1:3776269/1‑93 (MQ=255)

                                                                                      ttAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACt    >  2:2671931/1‑93 (MQ=255)

                                                                                      ttAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACt    >  2:3087742/1‑93 (MQ=255)

                                                                                       tAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTg   >  1:1505255/1‑93 (MQ=255)

                                                                                        aGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGt  <  2:468655/93‑1 (MQ=255)

ATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGT  >  NC_000913/3195753‑3195933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`