BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,194	C→T	V440V (GTC→GTT)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,194	0	C	T	84.9%	765.3 / 88.8	278	V440V (GTC→GTT)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (16/26); new base T (109/127); total (125/153)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 293 total aligned reads displayed.

TTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGAAGC  >  NC_000913/3225104‑3225283

ttCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtgg                                                                                         <  2:2518084/93‑1 (MQ=255)

 tCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggc                                                                                        >  1:5438208/1‑93 (MQ=255)

 tCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggc                                                                                        >  2:3285858/1‑93 (MQ=255)

 tCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggc                                                                                        >  2:3516180/1‑93 (MQ=255)

 tCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTcggc                                                                                        <  2:3629483/93‑1 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCTGAATATTCTGACCATCATTTTTTATAACGTtggcg                                                                                       >  1:3711647/1‑93 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggcgg                                                                                      >  2:7317125/1‑93 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggcgg                                                                                      >  1:6017469/1‑93 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTtggcgg                                                                                      >  2:2572714/1‑93 (MQ=255)

    aTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGt                                                                                     >  1:5933949/1‑93 (MQ=255)

    aTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGt                                                                                     >  1:3692888/1‑93 (MQ=255)

     tGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTa                                                                                    <  2:825382/93‑1 (MQ=255)

      gCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTAt                                                                                   <  2:1727722/93‑1 (MQ=255)

        tGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTg                                                                                 <  2:739693/93‑1 (MQ=255)

          aTTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGtt                                                                               >  1:406764/1‑93 (MQ=255)

          aTTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGtt                                                                               >  1:760010/1‑93 (MQ=255)

           ttGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTa                                                                              <  2:1045887/93‑1 (MQ=255)

            tGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTAt                                                                             >  1:6174024/1‑93 (MQ=255)

             gCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATc                                                                            <  2:5881760/93‑1 (MQ=255)

              cGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCt                                                                           <  2:4729079/93‑1 (MQ=255)

               gATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCtt                                                                          <  1:5626070/93‑1 (MQ=255)

                atatTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTc                                                                         >  2:5619139/1‑93 (MQ=255)

                  atTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCt                                                                       <  2:4905619/93‑1 (MQ=255)

                  atTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCt                                                                       <  2:2902822/93‑1 (MQ=255)

                   tttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCtt                                                                      >  2:3324359/1‑93 (MQ=255)

                   tttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCtt                                                                      >  2:2749286/1‑93 (MQ=255)

                   tttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCtt                                                                      >  1:6275765/1‑93 (MQ=255)

                   tttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCtt                                                                      >  2:4496576/1‑93 (MQ=255)

                    ttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTg                                                                     <  2:4290313/93‑1 (MQ=255)

                         gTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCttt                                                                >  1:4726359/1‑93 (MQ=255)

                          tAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTg                                                               <  2:2405453/93‑1 (MQ=255)

                          tAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTg                                                               >  1:5305246/1‑93 (MQ=255)

                           aGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGc                                                              <  1:5239391/93‑1 (MQ=255)

                           aGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGc                                                              <  1:2521462/93‑1 (MQ=255)

                            gggTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCg                                                             >  2:5403533/1‑93 (MQ=255)

                              gTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtg                                                           <  1:1063031/93‑1 (MQ=255)

                              gTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtg                                                           >  2:5785695/1‑93 (MQ=255)

                              gTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtg                                                           <  1:901222/93‑1 (MQ=255)

                               tttGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtgg                                                          <  2:2112612/93‑1 (MQ=255)

                               tttGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtgg                                                          <  1:464327/93‑1 (MQ=255)

                                  gTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGtggtgg                                                       >  2:441054/1‑93 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                      >  1:1318221/1‑93 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                      >  1:3861538/1‑93 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                      <  1:4868053/93‑1 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                      >  1:3036739/1‑93 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                      >  2:6595523/1‑93 (MQ=255)

                                    cGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGaa                                                     <  1:5965506/93‑1 (MQ=255)

                                     gCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGaaa                                                    >  2:1368075/1‑93 (MQ=255)

                                      cTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAAt                                                   >  1:6727147/1‑93 (MQ=255)

                                       ttCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATa                                                  >  1:1474568/1‑93 (MQ=255)

                                       ttCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATa                                                  <  2:1239183/93‑1 (MQ=255)

                                        tCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATAc                                                 <  2:3148468/93‑1 (MQ=255)

                                          gACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAg                                               <  1:4218595/93‑1 (MQ=255)

                                          gACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAg                                               <  1:4109061/93‑1 (MQ=255)

                                           aCATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGt                                              <  1:2221999/93‑1 (MQ=255)

                                             aTTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaa                                            <  1:3838204/93‑1 (MQ=255)

                                              ttCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaaa                                           <  1:2732174/93‑1 (MQ=255)

                                              ttCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaaa                                           >  1:493285/1‑93 (MQ=255)

                                               tCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAat                                          <  2:494784/93‑1 (MQ=255)

                                               tCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAat                                          >  1:3324809/1‑93 (MQ=255)

                                               tCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAat                                          <  1:4420469/93‑1 (MQ=255)

                                                cccGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAata                                         <  1:3124373/93‑1 (MQ=255)

                                                 ccGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAatat                                        >  1:2428864/1‑93 (MQ=255)

                                                    aCTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAAtatata                                     >  2:2353824/1‑93 (MQ=255)

                                                     cTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaa                                    <  2:1559698/93‑1 (MQ=255)

                                                     cTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaa                                    >  1:7315401/1‑93 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   <  2:2249426/93‑1 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   >  1:5137980/1‑93 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   <  1:4356479/93‑1 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   <  1:362887/93‑1 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   <  2:6747474/93‑1 (MQ=255)

                                                      tGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaaa                                   <  1:6782475/93‑1 (MQ=255)

                                                       ggCGC‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAg                                  >  1:5057349/1‑93 (MQ=255)

                                                        gcgc‑GAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAgg                                 <  2:389808/93‑1 (MQ=255)

                                                         cgc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAggg                                <  2:2393582/93‑1 (MQ=255)

                                                         cgc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAggg                                <  2:1409086/93‑1 (MQ=255)

                                                          gc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                               >  1:1814835/1‑93 (MQ=255)

                                                          gc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                               >  2:5538259/1‑93 (MQ=255)

                                                          gc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                               >  2:3027492/1‑93 (MQ=255)

                                                          gc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                               >  2:4170286/1‑93 (MQ=255)

                                                          gc‑gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                               <  1:7046265/93‑1 (MQ=255)

                                                             gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGAtt                             <  1:3545096/93‑1 (MQ=255)

                                                             gAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGAtc                             >  1:710614/1‑92 (MQ=255)

                                                             gAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGAtt                             <  1:3972986/93‑1 (MQ=255)

                                                               atatTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTaa                           >  2:371641/1‑93 (MQ=255)

                                                               atatTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTaa                           >  2:5303372/1‑93 (MQ=255)

                                                               atatTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTaa                           <  2:2045964/93‑1 (MQ=255)

                                                               atatTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTaa                           >  1:6537314/1‑93 (MQ=255)

                                                                 atTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACg                         >  1:6602894/1‑93 (MQ=255)

                                                                 atTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACg                         <  2:1888855/93‑1 (MQ=255)

                                                                  ttCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACgg                        <  1:2474694/93‑1 (MQ=255)

                                                                     tGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTg                     >  2:3946971/1‑93 (MQ=255)

                                                                      gACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGa                    >  2:4620792/1‑93 (MQ=255)

                                                                        ccATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAag                  <  2:515569/93‑1 (MQ=255)

                                                                         catcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAaga                 <  2:7079213/93‑1 (MQ=255)

                                                                          atcatTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAagag                >  1:1151233/1‑93 (MQ=255)

                                                                                  ttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCatat        <  1:4329499/93‑1 (MQ=255)

                                                                                     taACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGa     <  1:1750407/93‑1 (MQ=255)

                                                                                      aaCGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGaa    <  2:1456880/93‑1 (MQ=255)

                                                                                      aaCGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGaa    <  2:6864398/93‑1 (MQ=255)

TTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGC‑GAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGAAGC  >  NC_000913/3225104‑3225283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`