BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,407	A→G	G142G (GGA→GGG)	ykgG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,407	0	A	G	82.9%	572.2 / 118.6	270	G142G (GGA→GGG)	ykgG	putative transporter
Reads supporting (aligned to +/- strand): ref base A (19/27); new base G (113/110); total (132/138)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 275 total aligned reads displayed.

GGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCC  >  NC_000913/323316‑323498

gggATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAg                                                                                            >  2:4492276/1‑93 (MQ=255)

  gATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGc                                                                                          <  1:7276353/93‑1 (MQ=255)

   aTCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCg                                                                                         >  2:6285741/1‑93 (MQ=255)

    tCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGt                                                                                        <  1:883254/93‑1 (MQ=255)

     ccGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTg                                                                                       <  1:6842872/93‑1 (MQ=255)

      cGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTgg                                                                                      <  1:5996008/93‑1 (MQ=255)

       ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGt                                                                                     >  2:2764480/1‑93 (MQ=255)

       ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGt                                                                                     <  2:678356/93‑1 (MQ=255)

        gCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGtt                                                                                    <  1:3434469/93‑1 (MQ=255)

         cGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTc                                                                                   <  1:5591143/93‑1 (MQ=255)

           aaaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                                 >  1:6259208/1‑93 (MQ=255)

           aaaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                                 >  1:5302041/1‑93 (MQ=255)

            aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttt                                                                                >  1:2825835/1‑93 (MQ=255)

             aGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtttt                                                                               <  2:5867786/93‑1 (MQ=255)

              ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttttt                                                                              <  2:4173467/93‑1 (MQ=255)

                  ccGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccg                                                                          <  2:4057314/93‑1 (MQ=255)

                    gagaATATCTCGCAGGCAGGGCAGGCTAAAGGGGGGGTTGGGTATGCTGAATATGGTTTAACCGAATCGGGGGGGGGGGTTCTTTTTTccccc                                                                        >  1:3669513/1‑93 (MQ=255)

                    gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                        >  2:932058/1‑93 (MQ=255)

                    gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                        >  2:686881/1‑93 (MQ=255)

                    gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                        <  2:2195440/93‑1 (MQ=255)

                     agaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGGGGTTCTTTTTTccgccg                                                                       >  1:2683461/1‑93 (MQ=255)

                       aaTATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTccgccgcc                                                                     <  2:2590504/93‑1 (MQ=255)

                        atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgccg                                                                    >  1:1979235/1‑93 (MQ=255)

                         tatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGa                                                                   <  2:4442811/93‑1 (MQ=255)

                          atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                  >  1:7032813/1‑93 (MQ=255)

                          atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                  <  1:2184897/93‑1 (MQ=255)

                          atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                  <  2:5036909/93‑1 (MQ=255)

                           tctcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgc                                                                 >  1:6350036/1‑93 (MQ=255)

                           tctcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAgc                                                                 <  2:5544321/93‑1 (MQ=255)

                             tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                               <  1:3658975/93‑1 (MQ=255)

                             tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                               >  2:4658467/1‑93 (MQ=255)

                               gcaggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCgg                                                             <  1:2933943/93‑1 (MQ=255)

                                 aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGc                                                           >  2:1485494/1‑93 (MQ=255)

                                 aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGc                                                           >  2:129837/1‑93 (MQ=255)

                                  ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                          >  1:1949257/1‑93 (MQ=255)

                                   gcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGt                                                         <  2:5730960/93‑1 (MQ=255)

                                      gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                      <  2:2648765/93‑1 (MQ=255)

                                      gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                      >  1:1765024/1‑93 (MQ=255)

                                        gCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAtt                                                    >  2:141174/1‑93 (MQ=255)

                                          aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                  >  1:3611244/1‑93 (MQ=255)

                                          aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                  <  2:2929687/93‑1 (MQ=255)

                                           ggCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGGGTTCATTGAg                                                 >  1:2432931/1‑93 (MQ=255)

                                             cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                               <  2:6062374/93‑1 (MQ=255)

                                             cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                               <  2:7342258/93‑1 (MQ=255)

                                             cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                               <  1:1217784/93‑1 (MQ=255)

                                              tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                              <  2:5446502/93‑1 (MQ=255)

                                                aaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGc                                            <  1:3301899/93‑1 (MQ=255)

                                                aaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGc                                            <  2:1548792/93‑1 (MQ=255)

                                                  gTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTc                                          >  1:4488680/1‑93 (MQ=255)

                                                  gTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTc                                          <  2:7189217/93‑1 (MQ=255)

                                                    gggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTccc                                        <  1:5750708/93‑1 (MQ=255)

                                                      gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCgg                                      >  2:1228954/1‑93 (MQ=255)

                                                      gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCgg                                      <  1:4278034/93‑1 (MQ=255)

                                                      gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCgg                                      >  2:695172/1‑93 (MQ=255)

                                                       tgttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGa                                     >  2:3863768/1‑93 (MQ=255)

                                                        gttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGaa                                    >  1:320338/1‑93 (MQ=255)

                                                        gttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGaa                                    >  2:6091165/1‑93 (MQ=255)

                                                        gttgtGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGaa                                    >  1:1106249/1‑93 (MQ=255)

                                                          tgtgTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAata                                  <  1:4904364/93‑1 (MQ=255)

                                                           gtgtATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAatat                                 >  2:7118432/1‑93 (MQ=255)

                                                            tgtATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATAtt                                <  1:6024794/93‑1 (MQ=255)

                                                              tATGCTGAATATGGTTTAACCGAATCGGG‑GGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctc                             <  1:5104335/93‑1 (MQ=255)

                                                               aTGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctc                             <  2:6069855/93‑1 (MQ=255)

                                                               aTGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctc                             <  2:1997699/93‑1 (MQ=255)

                                                                tGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTctct                            <  2:1732834/93‑1 (MQ=255)

                                                                 gCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCtt                           >  1:2971415/1‑93 (MQ=255)

                                                                  cTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCttt                          >  2:7502650/1‑93 (MQ=255)

                                                                  cTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCttt                          <  1:5826530/93‑1 (MQ=255)

                                                                   tGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCtttt                         <  2:4876088/93‑1 (MQ=255)

                                                                    gAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCttttt                        >  1:391477/1‑93 (MQ=255)

                                                                     aaTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTa                       >  1:1046155/1‑93 (MQ=255)

                                                                      atatGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTAt                      >  2:6597969/1‑93 (MQ=255)

                                                                         tGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCt                   >  2:3706977/1‑93 (MQ=255)

                                                                         tGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCt                   >  1:6989194/1‑93 (MQ=255)

                                                                         tGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCt                   <  2:2297847/93‑1 (MQ=255)

                                                                          ggTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTg                  <  1:825180/93‑1 (MQ=255)

                                                                          ggTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTg                  <  1:6424443/93‑1 (MQ=255)

                                                                           gTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc                 <  1:2355461/93‑1 (MQ=255)

                                                                           gTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc                 >  1:3244824/1‑93 (MQ=255)

                                                                           gTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc                 <  2:4473815/93‑1 (MQ=255)

                                                                           gTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc                 <  2:394609/93‑1 (MQ=255)

                                                                            tttAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCg                <  2:1894803/93‑1 (MQ=255)

                                                                            tttAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCg                >  1:3178394/1‑93 (MQ=255)

                                                                            tttAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCg                >  1:737327/1‑93 (MQ=255)

                                                                             ttAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGt               >  2:1107055/1‑93 (MQ=255)

                                                                              tAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTa              >  2:3478476/1‑93 (MQ=255)

                                                                               aaCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaa             <  2:7407770/93‑1 (MQ=255)

                                                                                aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa            >  2:484822/1‑93 (MQ=255)

                                                                                aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa            >  2:6372544/1‑93 (MQ=255)

                                                                                aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa            >  1:401965/1‑93 (MQ=255)

                                                                                aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa            >  2:3414851/1‑93 (MQ=255)

                                                                                aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa            >  2:1168835/1‑93 (MQ=255)

                                                                                 ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa           <  1:4220164/93‑1 (MQ=255)

                                                                                 ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa           >  2:5266605/1‑93 (MQ=255)

                                                                                 ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa           <  1:6302501/93‑1 (MQ=255)

                                                                                      tCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACt      >  1:5339553/1‑93 (MQ=255)

                                                                                      tCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACt      <  2:3108557/93‑1 (MQ=255)

                                                                                       cGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa     <  1:6716477/93‑1 (MQ=255)

                                                                                       cGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa     <  1:1624684/93‑1 (MQ=255)

                                                                                         ggAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATc   >  2:7256896/1‑93 (MQ=255)

GGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCC  >  NC_000913/323316‑323498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`