BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,242,993	A→G	T84A (ACC→GCC)	exuT →	ExuT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,242,993	0	A	G	87.6%	780.3 / 83.2	314	T84A (ACC→GCC)	exuT	ExuT
Reads supporting (aligned to +/- strand): ref base A (21/18); new base G (134/141); total (155/159)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 314 total aligned reads displayed.

CTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGT  >  NC_000913/3242903‑3243082

cTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGcc                                                                                         >  2:2948677/1‑93 (MQ=255)

cTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGcc                                                                                         <  1:817520/93‑1 (MQ=255)

 tGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCAccc                                                                                        <  1:2059133/93‑1 (MQ=255)

    tGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAAc                                                                                     >  1:7447831/1‑93 (MQ=255)

    tGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAAc                                                                                     >  1:3070037/1‑93 (MQ=255)

       cGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGt                                                                                  <  2:6694579/93‑1 (MQ=255)

        gCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTa                                                                                 <  2:5235005/93‑1 (MQ=255)

        gCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTa                                                                                 <  1:5878878/93‑1 (MQ=255)

        gCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTa                                                                                 <  1:3364209/93‑1 (MQ=255)

         cTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTAt                                                                                <  1:1832680/93‑1 (MQ=255)

         cTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTAt                                                                                <  1:3730305/93‑1 (MQ=255)

          tCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTAtt                                                                               <  1:5716137/93‑1 (MQ=255)

          tCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAtt                                                                               <  2:2580132/93‑1 (MQ=255)

           cGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTc                                                                              <  1:1374835/93‑1 (MQ=255)

           cGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTc                                                                              <  2:6552777/93‑1 (MQ=255)

            ggCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGGAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTcc                                                                             >  1:6185815/1‑93 (MQ=255)

            ggCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTcc                                                                             <  2:5628170/93‑1 (MQ=255)

            ggCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTcc                                                                             <  2:314587/93‑1 (MQ=255)

             gCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCt                                                                            <  2:1714052/93‑1 (MQ=255)

              cACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCta                                                                           <  2:7503370/93‑1 (MQ=255)

              cACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCta                                                                           <  2:5487878/93‑1 (MQ=255)

               aCCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtat                                                                          <  2:6170041/93‑1 (MQ=255)

               aCCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtat                                                                          >  2:3937554/1‑93 (MQ=255)

               aCCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtat                                                                          >  2:6981178/1‑93 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtata                                                                         >  1:5331442/1‑93 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtata                                                                         <  1:934162/93‑1 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtata                                                                         >  2:3430293/1‑93 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtata                                                                         <  2:4105748/93‑1 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCtata                                                                         >  2:573057/1‑93 (MQ=255)

                ccGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTCATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCtata                                                                         >  2:2630516/1‑93 (MQ=255)

                  gTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATatc                                                                       <  2:3855651/93‑1 (MQ=255)

                   tGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATatca                                                                      <  1:2541357/93‑1 (MQ=255)

                     cTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATatcatc                                                                    <  1:5239056/93‑1 (MQ=255)

                     cTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATatcatc                                                                    >  2:1892808/1‑93 (MQ=255)

                     cTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATatcatc                                                                    <  2:6214075/93‑1 (MQ=255)

                      ttGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCg                                                                   >  2:2811136/1‑93 (MQ=255)

                          ttACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGc                                                               <  1:6681515/93‑1 (MQ=255)

                             ccTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTa                                                            <  1:6593556/93‑1 (MQ=255)

                              cTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTAt                                                           >  2:2494237/1‑93 (MQ=255)

                              cTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTAt                                                           >  2:5330052/1‑93 (MQ=255)

                               tGACGCGTAACACTGTGGCGGCAGCTTCGCCAACTCTGATGGGAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATTATTGCCGCCTAtt                                                          >  1:4287587/1‑93 (MQ=255)

                                gACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTc                                                         <  2:555114/93‑1 (MQ=255)

                                 aCGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTct                                                        <  1:3511374/93‑1 (MQ=255)

                                 aCGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTct                                                        <  1:3972413/93‑1 (MQ=255)

                                 aCGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTACATCATCGCAGCCTATTct                                                        >  1:3480001/1‑93 (MQ=255)

                                   gcgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTctgc                                                      >  1:4657051/1‑93 (MQ=255)

                                      tAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCtgctgc                                                   <  1:4550439/93‑1 (MQ=255)

                                       aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTgctgct                                                  <  2:3352677/93‑1 (MQ=255)

                                           cTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTtata                                              <  1:6063354/93‑1 (MQ=255)

                                           cTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGATGCTtata                                              <  2:6668164/93‑1 (MQ=255)

                                           cTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTtata                                              >  2:801200/1‑93 (MQ=255)

                                               ggcggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt                                          <  1:2267333/93‑1 (MQ=255)

                                               ggcggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt                                          <  1:4907335/93‑1 (MQ=255)

                                               ggcggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt                                          <  1:188884/93‑1 (MQ=255)

                                                 cggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGCTCa                                        <  1:6974122/93‑1 (MQ=255)

                                                  ggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCAt                                       <  1:3576899/93‑1 (MQ=255)

                                                      gCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCaa                                   >  1:1657239/1‑93 (MQ=255)

                                                      gCTGCGCCAACTCTGATGGAAGAGCTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCaa                                   >  1:3907764/1‑93 (MQ=255)

                                                       cTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAAc                                  >  2:6373770/1‑93 (MQ=255)

                                                        tGCGCCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAAcc                                 <  1:1182360/93‑1 (MQ=255)

                                                          cgcCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                               <  2:7254433/93‑1 (MQ=255)

                                                           gcCAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCCGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt                              <  2:4521300/93‑1 (MQ=255)

                                                            ccAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTa                             <  1:1669270/93‑1 (MQ=255)

                                                            ccAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTa                             >  2:3023386/1‑93 (MQ=255)

                                                            ccAACTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTa                             >  1:5101311/1‑93 (MQ=255)

                                                               aCTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCa                          >  1:6548727/1‑93 (MQ=255)

                                                               aCTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCa                          >  2:4264607/1‑93 (MQ=255)

                                                               aCTCTGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCa                          >  1:4478806/1‑93 (MQ=255)

                                                                ctctGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAg                         >  2:4819723/1‑93 (MQ=255)

                                                                 tctGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAgg                        >  1:1570167/1‑93 (MQ=255)

                                                                 tctGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAgg                        >  2:6327357/1‑93 (MQ=255)

                                                                  ctGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGt                       >  1:7101933/1‑93 (MQ=255)

                                                                   tGATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGtt                      >  1:2672254/1‑93 (MQ=255)

                                                                    gATGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa                     <  2:3314778/93‑1 (MQ=255)

                                                                    gATGGAAGAGTTAAACATCTCCGCACAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa                     >  2:7362994/1‑93 (MQ=255)

                                                                    gATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa                     <  2:6817007/93‑1 (MQ=255)

                                                                     aTGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTAt                    <  2:215189/93‑1 (MQ=255)

                                                                      tGGAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATg                   >  1:2547557/1‑93 (MQ=255)

                                                                      tGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATg                   >  1:5519207/1‑93 (MQ=255)

                                                                       ggAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt                  >  1:4865140/1‑93 (MQ=255)

                                                                       ggAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt                  >  1:2840114/1‑93 (MQ=255)

                                                                       ggAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt                  <  1:6267656/93‑1 (MQ=255)

                                                                       ggAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt                  <  1:2164806/93‑1 (MQ=255)

                                                                        gAAGAGTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa                 >  1:5069356/1‑93 (MQ=255)

                                                                          agagTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACt               >  1:3256103/1‑93 (MQ=255)

                                                                            agTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTgg             <  2:153148/93‑1 (MQ=255)

                                                                             gTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGa            <  1:7368789/93‑1 (MQ=255)

                                                                             gTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGa            <  1:5630645/93‑1 (MQ=255)

                                                                             gTTAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGa            <  2:4925161/93‑1 (MQ=255)

                                                                             gTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGa            >  1:1975880/1‑93 (MQ=255)

                                                                               tAAACATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGAtg          >  1:4072517/1‑93 (MQ=255)

                                                                                aaaCATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGAtgt         <  2:1327808/93‑1 (MQ=255)

                                                                                 aaCATCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGAtgtg        >  2:3733307/1‑93 (MQ=255)

                                                                                  aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGc       <  2:4718086/93‑1 (MQ=255)

                                                                                    aTCTCCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTg     >  2:5940704/1‑93 (MQ=255)

                                                                                     tctcCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTgg    <  1:4149154/93‑1 (MQ=255)

                                                                                     tctcCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTgg    >  2:5051715/1‑93 (MQ=255)

                                                                                      ctcCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTggg   <  2:7127216/93‑1 (MQ=255)

                                                                                       tcCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGt  >  2:206796/1‑93 (MQ=255)

                                                                                       tcCGCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGt  <  2:4580028/93‑1 (MQ=255)

CTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGT  >  NC_000913/3242903‑3243082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`