BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,717	A→G	noncoding (83/87 nt)	leuU ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,717	0	A	G	85.2%	754.4 / 122.0	325	noncoding (83/87 nt)	leuU	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (31/17); new base G (175/102); total (206/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 325 total aligned reads displayed.

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

    gagaTACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAgg                                                                                     <  2:5203186/93‑1 (MQ=255)

     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                    <  2:3233775/93‑1 (MQ=255)

     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                    <  2:3565854/93‑1 (MQ=255)

      gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAc                                                                                   >  1:3622355/1‑93 (MQ=255)

       atacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACg                                                                                  <  2:568014/93‑1 (MQ=255)

         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                >  1:6621241/1‑93 (MQ=255)

         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACggg                                                                                >  2:5374332/1‑93 (MQ=255)

          cagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGa                                                                               >  2:2606229/1‑93 (MQ=255)

           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              >  1:4137854/1‑93 (MQ=255)

            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  1:7423207/1‑93 (MQ=255)

            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  2:2277965/1‑93 (MQ=255)

              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           <  1:6928968/93‑1 (MQ=255)

              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           >  1:6174704/1‑93 (MQ=255)

              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           >  2:678904/1‑93 (MQ=255)

              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTg                                                                           >  1:5760875/1‑93 (MQ=255)

                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         >  1:7218077/1‑93 (MQ=255)

                  tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTAGTACCGAGGACGGGACTTGAAcc                                                                       <  2:7481972/93‑1 (MQ=255)

                      aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTa                                                                   >  2:3206300/1‑93 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:3068902/1‑93 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  <  2:6118660/93‑1 (MQ=255)

                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:1002680/1‑93 (MQ=255)

                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  1:107829/93‑1 (MQ=255)

                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  2:4127488/93‑1 (MQ=255)

                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  1:776510/93‑1 (MQ=255)

                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  2:4429740/93‑1 (MQ=255)

                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  2:2803944/93‑1 (MQ=255)

                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  1:2489910/93‑1 (MQ=255)

                              cTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAt                                                           <  2:1102954/93‑1 (MQ=255)

                              cTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTAt                                                           <  2:3470529/93‑1 (MQ=255)

                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          <  1:4761416/93‑1 (MQ=255)

                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          >  2:675710/1‑93 (MQ=255)

                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          >  1:6088243/1‑93 (MQ=255)

                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         <  2:5543745/93‑1 (MQ=255)

                                 aGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                        >  2:2520091/1‑93 (MQ=255)

                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      >  1:1864344/1‑93 (MQ=255)

                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      <  2:6838300/93‑1 (MQ=255)

                                    ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCa                                                     >  2:6324073/1‑93 (MQ=255)

                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                    >  1:267072/1‑93 (MQ=255)

                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                    >  2:3693730/1‑93 (MQ=255)

                                       ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                  >  1:3260372/1‑93 (MQ=255)

                                        gACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                 >  1:1730008/1‑93 (MQ=255)

                                        gACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                 <  2:6941721/93‑1 (MQ=255)

                                         aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                >  1:1988234/1‑93 (MQ=255)

                                         aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                >  1:7092048/1‑93 (MQ=255)

                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacca                                               <  1:6345037/93‑1 (MQ=255)

                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAAGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacca                                               <  2:2935999/93‑1 (MQ=255)

                                            aaaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                             >  2:6393444/1‑93 (MQ=255)

                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  1:1916568/1‑93 (MQ=255)

                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  2:3044814/1‑93 (MQ=255)

                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  2:472499/1‑93 (MQ=255)

                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          <  1:6696201/93‑1 (MQ=255)

                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          >  1:1708712/1‑93 (MQ=255)

                                                 gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                        >  2:4080675/1‑93 (MQ=255)

                                                 gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                        >  1:2307666/1‑93 (MQ=255)

                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  1:3749678/93‑1 (MQ=255)

                                                    aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                     >  1:3540079/1‑93 (MQ=255)

                                                      aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                   >  1:6759482/1‑93 (MQ=255)

                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  2:1424593/1‑93 (MQ=255)

                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  1:5028565/1‑93 (MQ=255)

                                                        gACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                 >  2:390735/1‑93 (MQ=255)

                                                         aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                <  2:7164868/93‑1 (MQ=255)

                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               >  1:1061445/1‑93 (MQ=255)

                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  2:1750028/1‑93 (MQ=255)

                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:2105141/1‑93 (MQ=255)

                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            <  2:6558361/93‑1 (MQ=255)

                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:2557175/1‑93 (MQ=255)

                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:5653800/1‑93 (MQ=255)

                                                              tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                           >  1:4593391/1‑93 (MQ=255)

                                                                  aGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                       <  2:729440/93‑1 (MQ=255)

                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      <  2:6220117/93‑1 (MQ=255)

                                                                    cGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTc                     <  2:3305950/93‑1 (MQ=255)

                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    >  1:821472/1‑93 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   <  2:7035548/93‑1 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:489807/1‑93 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:4569636/1‑93 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:6489150/1‑93 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  1:593814/1‑93 (MQ=255)

                                                                       ctctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  >  2:1906957/1‑93 (MQ=255)

                                                                       ctctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  <  1:3882726/93‑1 (MQ=255)

                                                                       ctctTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  <  1:2182714/93‑1 (MQ=255)

                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:4242344/1‑93 (MQ=255)

                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:525132/1‑93 (MQ=255)

                                                                          ttttCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCcaccac               >  1:1599575/1‑93 (MQ=255)

                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  2:1959633/93‑1 (MQ=255)

                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             >  1:1106666/1‑93 (MQ=255)

                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  2:6902387/93‑1 (MQ=255)

                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  2:1258844/1‑93 (MQ=255)

                                                                              cTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  1:7339762/1‑93 (MQ=255)

                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         >  2:5899767/1‑93 (MQ=255)

                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         <  1:1394014/93‑1 (MQ=255)

                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         >  1:3374639/1‑93 (MQ=255)

                                                                                 gAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCa        <  2:7392473/93‑1 (MQ=255)

                                                                                 gAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCa        <  1:1524752/93‑1 (MQ=255)

                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:6506090/1‑93 (MQ=255)

                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  1:571937/1‑93 (MQ=255)

                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACAACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       <  2:6007163/93‑1 (MQ=255)

                                                                                  aaTTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:836231/1‑93 (MQ=255)

                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  1:3135754/1‑93 (MQ=255)

                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  2:4355388/1‑93 (MQ=255)

                                                                                    tttGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACgg     >  1:519304/1‑93 (MQ=255)

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`