BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,324,185	G→A	A131V (GCC→GTC)	hflB ←	HflB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,324,185	0	G	A	89.5%	914.6 / 61.4	306	A131V (GCC→GTC)	hflB	HflB
Reads supporting (aligned to +/- strand): ref base G (14/18); new base A (143/130); total (157/149)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 312 total aligned reads displayed.

TTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATA  >  NC_000913/3324096‑3324274

ttGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGacac                                                                                         <  2:5120381/93‑1 (MQ=255)

 tGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACAcc                                                                                        <  2:2755123/93‑1 (MQ=255)

 tGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACAcc                                                                                        <  1:5839144/93‑1 (MQ=255)

  gCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCt                                                                                       <  2:5067554/93‑1 (MQ=255)

   cTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCtt                                                                                      <  1:784870/93‑1 (MQ=255)

   cTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCtt                                                                                      >  1:7323728/1‑93 (MQ=255)

    ttCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCttt                                                                                     >  1:6897581/1‑93 (MQ=255)

    ttCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCttt                                                                                     >  2:6577977/1‑93 (MQ=255)

     tcgtcgCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTg                                                                                    <  1:2503662/93‑1 (MQ=255)

         cgCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑c                                                                               <  2:1394047/93‑1 (MQ=255)

         cgCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑c                                                                               <  1:4373993/93‑1 (MQ=255)

          gCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGACCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑cc                                                                              >  1:1264183/1‑93 (MQ=255)

            aGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑ccgc                                                                            <  1:3406286/93‑1 (MQ=255)

             gCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑ccgcc                                                                           >  1:4629730/1‑93 (MQ=255)

               ccGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑Ccgccgc                                                                         >  2:25048/1‑93 (MQ=255)

                cGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCgccgcc                                                                        >  1:2855983/1‑93 (MQ=255)

                cGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCgccgcc                                                                        >  1:6845093/1‑93 (MQ=255)

                 gCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGccc                                                                       <  1:6805092/93‑1 (MQ=255)

                 gCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGccc                                                                       >  1:1059886/1‑93 (MQ=255)

                  cAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCt                                                                      <  1:4819839/93‑1 (MQ=255)

                  cAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCt                                                                      <  2:2329886/93‑1 (MQ=255)

                  cAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCt                                                                      <  2:1235578/93‑1 (MQ=255)

                   aaCGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTg                                                                     >  2:5851053/1‑93 (MQ=255)

                   aaCGTCAGCAAAGGTCGATTTGATCTGATCTTACGACAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTg                                                                     >  1:5704330/1‑93 (MQ=255)

                    aCGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGc                                                                    >  1:517962/1‑93 (MQ=255)

                    aCGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGc                                                                    >  2:5932331/1‑93 (MQ=255)

                     cGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCa                                                                   <  2:5693439/93‑1 (MQ=255)

                     cGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCa                                                                   <  1:1699602/93‑1 (MQ=255)

                     cGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCa                                                                   >  2:613615/1‑93 (MQ=255)

                      gTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCAt                                                                  >  2:4125876/1‑93 (MQ=255)

                        cAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCAttt                                                                <  2:6847986/93‑1 (MQ=255)

                         aGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTg                                                               >  2:4229003/1‑93 (MQ=255)

                          gCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGa                                                              <  1:3303356/93‑1 (MQ=255)

                          gCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGa                                                              <  1:1981424/93‑1 (MQ=255)

                             aaGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGc                                                           >  1:3798998/1‑93 (MQ=255)

                             aaGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGc                                                           >  1:1582889/1‑93 (MQ=255)

                              aGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCa                                                          >  1:3707189/1‑93 (MQ=255)

                              aGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCa                                                          <  1:1289388/93‑1 (MQ=255)

                               ggTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCAt                                                         <  2:5460193/93‑1 (MQ=255)

                                gTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATg                                                        >  1:6260523/1‑93 (MQ=255)

                                    ttttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATgaaga                                                    <  1:5122899/93‑1 (MQ=255)

                                     tttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATgaagaa                                                   >  1:493000/1‑93 (MQ=255)

                                     tttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATgaagaa                                                   <  2:7489489/93‑1 (MQ=255)

                                     tttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATgaagaa                                                   <  1:6072735/93‑1 (MQ=255)

                                     tttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATgaagaa                                                   <  1:5269150/93‑1 (MQ=255)

                                      ttGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGaagaag                                                  >  1:7096217/1‑93 (MQ=255)

                                       tgatctgatcTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAagaaga                                                 <  1:3237023/93‑1 (MQ=255)

                                       tgatctgatcTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAagaaga                                                 >  2:2227941/1‑93 (MQ=255)

                                        gatctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGAt                                                >  2:3789122/1‑93 (MQ=255)

                                        gatctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGAt                                                >  2:6330692/1‑93 (MQ=255)

                                         atctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATc                                               <  2:3248824/93‑1 (MQ=255)

                                         atctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATc                                               <  2:5325894/93‑1 (MQ=255)

                                          tctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATcc                                              <  1:724887/93‑1 (MQ=255)

                                           ctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATcga                                             <  2:3067092/93‑3 (MQ=255)

                                           ctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCa                                             >  2:2682621/1‑93 (MQ=255)

                                           ctgatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCa                                             <  2:7043178/93‑1 (MQ=255)

                                             gatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGa                                           <  2:4543967/93‑1 (MQ=255)

                                             gatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGa                                           >  2:5974530/1‑93 (MQ=255)

                                             gatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGa                                           >  2:833040/1‑93 (MQ=255)

                                             gatctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGa                                           <  1:6141688/93‑1 (MQ=255)

                                               tctTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGaca                                         >  2:1805600/1‑93 (MQ=255)

                                                      tCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCa                                  >  1:1728118/1‑93 (MQ=255)

                                                      tCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCa                                  <  2:1605228/93‑1 (MQ=255)

                                                      tCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCa                                  >  1:5984705/1‑93 (MQ=255)

                                                      tCAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCa                                  >  1:6559531/1‑93 (MQ=255)

                                                       cAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAg                                 <  1:2939084/93‑1 (MQ=255)

                                                       cAGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAg                                 >  1:7217482/1‑93 (MQ=255)

                                                        aGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGc                                >  2:2029848/1‑93 (MQ=255)

                                                        aGCATGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGc                                <  2:4053434/93‑1 (MQ=255)

                                                            tGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACa                            >  1:5081087/1‑93 (MQ=255)

                                                            tGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACa                            >  1:7059739/1‑93 (MQ=255)

                                                            tGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACa                            <  1:3061872/93‑1 (MQ=255)

                                                            tGCGCGCTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACa                            <  1:2569569/93‑1 (MQ=255)

                                                             gcgcgcTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAg                           <  2:3512218/93‑1 (MQ=255)

                                                              cgcgcTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGc                          <  1:6345531/93‑1 (MQ=255)

                                                                cgcTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCAt                        <  1:4513266/93‑1 (MQ=255)

                                                                 gcTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATc                       <  1:4991917/93‑1 (MQ=255)

                                                                 gcTTTGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATc                       <  1:4024618/93‑1 (MQ=255)

                                                                  cTTTGCTCTTACCAAACGACATGACACCTTTGCCATCCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATc                       <  1:1210676/93‑1 (MQ=255)

                                                                   tttGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCgg                     >  1:180888/1‑93 (MQ=255)

                                                                   tttGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCgg                     <  2:1856035/93‑1 (MQ=255)

                                                                   tttGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCgg                     <  2:7236495/93‑1 (MQ=255)

                                                                   tttGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCgg                     <  2:592812/93‑1 (MQ=255)

                                                                    ttGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCggg                    >  1:4647583/1‑93 (MQ=255)

                                                                    ttGCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCggg                    >  2:1109006/1‑93 (MQ=255)

                                                                      gCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGaa                  <  2:4706781/93‑1 (MQ=255)

                                                                      gCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGaa                  >  1:5387248/1‑93 (MQ=255)

                                                                      gCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGaa                  >  2:4280088/1‑93 (MQ=255)

                                                                      gCTCTTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGaa                  <  2:2868786/93‑1 (MQ=255)

                                                                        tctTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAAcc                <  2:1717919/93‑1 (MQ=255)

                                                                        tctTACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAAcc                >  2:5243983/1‑93 (MQ=255)

                                                                           tACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAgg             <  2:2469346/93‑1 (MQ=255)

                                                                           tACCAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAgg             >  1:5441729/1‑93 (MQ=255)

                                                                             ccAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGgag           <  1:2711672/93‑1 (MQ=255)

                                                                              cAAACGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGgaga          >  1:967828/1‑93 (MQ=255)

                                                                               aaaCGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGAt         <  1:2660627/93‑1 (MQ=255)

                                                                               aaaCGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGAt         <  2:2810068/93‑1 (MQ=255)

                                                                                  cGACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGaa      >  2:4377032/1‑93 (MQ=255)

                                                                                   gACATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAg     >  1:2120023/1‑93 (MQ=255)

                                                                                     cATGACACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGAt   >  1:7444297/1‑93 (MQ=255)

TTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCA‑CCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATA  >  NC_000913/3324096‑3324274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`