BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,412,612	A→G	Q371R (CAG→CGG)	acrE →	AcrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,412,612	0	A	G	84.5%	740.1 / 137.2	335	Q371R (CAG→CGG)	acrE	AcrE
Reads supporting (aligned to +/- strand): ref base A (24/28); new base G (129/154); total (153/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

Only 100 of 337 total aligned reads displayed.

GGCGATTGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGATATTTG  >  NC_000913/3412520‑3412703

ggCGATTGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCa                                                                                             <  1:1067231/93‑1 (MQ=255)

ggCGATTGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCa                                                                                             >  1:4873699/1‑93 (MQ=255)

   gATTGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCGAAAAGCGCGTCCGGGAGAGCGGGt                                                                                          <  2:3286718/93‑1 (MQ=255)

     ttGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTa                                                                                        >  2:268441/1‑93 (MQ=255)

         cGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGc                                                                                    <  2:2875192/93‑1 (MQ=255)

         cGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGc                                                                                    <  1:4591658/93‑1 (MQ=255)

          gATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGcc                                                                                   >  2:1126775/1‑93 (MQ=255)

          gATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGcc                                                                                   >  1:3389075/1‑93 (MQ=255)

            tAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCAc                                                                                 >  2:7165518/1‑93 (MQ=255)

            tAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCAc                                                                                 <  2:2598452/93‑1 (MQ=255)

              aaTGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTa                                                                               >  2:902954/1‑93 (MQ=255)

              aaTGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTa                                                                               <  1:6705005/93‑1 (MQ=255)

              aaTGGTTGATTACTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTa                                                                               <  1:6235013/93‑1 (MQ=255)

               acggTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTAc                                                                              <  2:1486864/91‑1 (MQ=255)

               aTGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTAc                                                                              <  2:4602629/93‑1 (MQ=255)

               aTGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTAc                                                                              >  2:5996212/1‑93 (MQ=255)

                tGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTAcc                                                                             <  1:3604822/93‑1 (MQ=255)

                 ggTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCg                                                                            >  1:5441945/1‑93 (MQ=255)

                  gTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGa                                                                           >  1:202288/1‑93 (MQ=255)

                   ttGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGAt                                                                          >  1:4758280/1‑93 (MQ=255)

                    tGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATa                                                                         <  2:5124755/93‑1 (MQ=255)

                       ttAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATAccc                                                                      >  1:7524737/1‑93 (MQ=255)

                        tAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATAcccc                                                                     <  1:3845796/93‑1 (MQ=255)

                        tAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATAcccc                                                                     <  2:4522328/93‑1 (MQ=255)

                        tAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATAcccc                                                                     >  2:4157475/1‑93 (MQ=255)

                           tGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGc                                                                  >  2:1331969/1‑93 (MQ=255)

                           tGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGc                                                                  >  1:2765418/1‑93 (MQ=255)

                           tGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGc                                                                  >  2:308850/1‑93 (MQ=255)

                             aaGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAg                                                                >  2:1006915/1‑93 (MQ=255)

                             aaGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAg                                                                >  1:2917534/1‑93 (MQ=255)

                              aGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGa                                                               <  1:4108691/93‑1 (MQ=255)

                              aGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGa                                                               <  2:4228904/93‑1 (MQ=255)

                               ggACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGAt                                                              <  1:4297043/93‑1 (MQ=255)

                               ggACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGAt                                                              >  1:1248655/1‑93 (MQ=255)

                                gACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATa                                                             >  2:5845259/1‑93 (MQ=255)

                                  cTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACt                                                           <  2:392835/93‑1 (MQ=255)

                                   tGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTg                                                          >  2:5281239/1‑93 (MQ=255)

                                   tGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTg                                                          >  2:624297/1‑93 (MQ=255)

                                   tGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTg                                                          <  1:5147098/93‑1 (MQ=255)

                                    gAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGc                                                         >  2:1365309/1‑93 (MQ=255)

                                    gAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGc                                                         >  2:6501394/1‑93 (MQ=255)

                                    gAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGc                                                         >  2:4904519/1‑93 (MQ=255)

                                    gAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGc                                                         >  1:1021596/1‑93 (MQ=255)

                                     aaaTCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCa                                                        <  1:6121854/93‑1 (MQ=255)

                                      aaTCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCAt                                                       <  2:3795453/93‑1 (MQ=255)

                                        tCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCg                                                     >  2:7349779/1‑93 (MQ=255)

                                         cTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGa                                                    <  1:3673457/93‑1 (MQ=255)

                                          tGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGaa                                                   >  2:2076064/1‑93 (MQ=255)

                                            gCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGt                                                 >  1:6925146/1‑93 (MQ=255)

                                            gCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGt                                                 >  1:1121537/1‑93 (MQ=255)

                                              gATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTaa                                               <  1:4652926/93‑1 (MQ=255)

                                               aTCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAg                                              <  2:5746581/93‑1 (MQ=255)

                                               aTCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAg                                              <  2:4490849/93‑1 (MQ=255)

                                               aTCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAg                                              >  2:1656649/1‑93 (MQ=255)

                                               aTCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAg                                              >  2:2370302/1‑93 (MQ=255)

                                                tCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAgg                                             <  1:784138/93‑1 (MQ=255)

                                                tCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAgg                                             >  2:3033535/1‑93 (MQ=255)

                                                tCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAgg                                             <  2:313306/93‑1 (MQ=255)

                                                  aaGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTa                                           <  1:7495540/93‑1 (MQ=255)

                                                  aaGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTa                                           >  2:686618/1‑93 (MQ=255)

                                                  aaGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTa                                           >  2:6306730/1‑93 (MQ=255)

                                                   aGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTaa                                          >  1:2334653/1‑93 (MQ=255)

                                                   aGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTaa                                          >  2:3556139/1‑93 (MQ=255)

                                                    gTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAAt                                         <  1:5999584/93‑1 (MQ=255)

                                                     tCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATc                                        >  2:5199880/1‑93 (MQ=255)

                                                      cATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCt                                       >  1:2478316/1‑93 (MQ=255)

                                                       aTTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTg                                      >  1:5112387/1‑93 (MQ=255)

                                                        ttGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGa                                     <  2:2719644/93‑1 (MQ=255)

                                                          gTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACa                                   <  1:923822/93‑1 (MQ=255)

                                                           tCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACAt                                  >  2:2960084/1‑93 (MQ=255)

                                                           tCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACAt                                  >  1:6487317/1‑93 (MQ=255)

                                                            cAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATg                                 <  2:1793865/93‑1 (MQ=255)

                                                             aGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATgg                                <  2:557769/93‑1 (MQ=255)

                                                              gCGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCGCTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGc                               <  1:326431/93‑1 (MQ=255)

                                                               cGGCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCa                              >  1:2544249/1‑93 (MQ=255)

                                                                 gCCTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCaaa                            <  1:2122371/93‑1 (MQ=255)

                                                                  ccTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAAc                           <  1:1429245/93‑1 (MQ=255)

                                                                   cTGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACt                          >  2:7230397/1‑93 (MQ=255)

                                                                   cTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACt                          >  2:5490170/1‑93 (MQ=255)

                                                                    tGCAAAAAGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACtt                         >  1:6857851/1‑93 (MQ=255)

                                                                      cAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACtttt                       >  1:4014773/1‑93 (MQ=255)

                                                                       aaaaaGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACttttt                      <  1:5193693/93‑1 (MQ=255)

                                                                        aaaaGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACtttttt                     >  1:4860944/1‑93 (MQ=255)

                                                                        aaaaGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACtttttt                     >  2:337308/1‑93 (MQ=255)

                                                                        aaaaGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACtttttt                     <  1:7334946/93‑1 (MQ=255)

                                                                          aaGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTAt                   <  2:6575423/93‑1 (MQ=255)

                                                                           aGCGCGTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTAtt                  <  2:4984322/93‑1 (MQ=255)

                                                                              gcgTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTcga               >  1:6188020/1‑93 (MQ=255)

                                                                               cgTCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTcgac              >  2:406780/1‑93 (MQ=255)

                                                                                 tCCGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTcgacga            >  1:5636761/1‑93 (MQ=255)

                                                                                  ccGGGAGAGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCgacgac           <  2:818538/93‑1 (MQ=255)

                                                                                      gagaGCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGat       >  1:7109568/1‑93 (MQ=255)

                                                                                       agagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGata      <  2:5314064/93‑1 (MQ=255)

                                                                                       agagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGata      <  1:4274674/93‑1 (MQ=255)

                                                                                       agagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGata      >  1:1492869/1‑93 (MQ=255)

                                                                                        gagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGatat     <  1:3920682/93‑1 (MQ=255)

                                                                                        gagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGatat     <  1:5259724/93‑1 (MQ=255)

                                                                                        gagCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGatat     <  1:2664615/93‑1 (MQ=255)

                                                                                         agCGGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGATAtt    <  1:1858700/93‑1 (MQ=255)

                                                                                          gCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGATAttt   <  2:2222980/93‑1 (MQ=255)

GGCGATTGGCGATAAATGGTTGATTAGTGAAGGACTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAGCCACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGATATTTG  >  NC_000913/3412520‑3412703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`