BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,723	T→C	F352L (TTC→CTC)	acrF →	AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,723	0	T	C	81.2%	642.9 / 153.4	309	F352L (TTC→CTC)	acrF	AcrF
Reads supporting (aligned to +/- strand): ref base T (33/25); new base C (142/109); total (175/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 313 total aligned reads displayed.

GAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGTTTGCC  >  NC_000913/3413632‑3413812

gAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGtt                                                                                          <  2:7031671/93‑1 (MQ=255)

  aGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTcc                                                                                        >  2:3595008/1‑93 (MQ=255)

  aGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTcc                                                                                        <  2:5606884/93‑1 (MQ=255)

  aGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTcc                                                                                        <  2:6235975/93‑1 (MQ=255)

    gNTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCtn                                                                                      <  2:5395358/93‑2 (MQ=255)

     ttCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTgg                                                                                     <  2:7240622/93‑1 (MQ=255)

     ttCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTgg                                                                                     >  1:3793390/1‑93 (MQ=255)

      tctcTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGt                                                                                    <  2:2359724/93‑1 (MQ=255)

       ctctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTg                                                                                   >  1:4690941/1‑93 (MQ=255)

        tctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGa                                                                                  <  2:4493972/93‑1 (MQ=255)

         ctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGAt                                                                                 <  1:6189032/93‑1 (MQ=255)

          tACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATg                                                                                >  2:4743603/1‑93 (MQ=255)

           aCCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGt                                                                               >  1:6989717/1‑93 (MQ=255)

           aCCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGt                                                                               <  1:2712299/93‑1 (MQ=255)

           aCCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGt                                                                               >  1:1712163/1‑93 (MQ=255)

            cccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTa                                                                              >  2:5490661/1‑93 (MQ=255)

            cccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTa                                                                              <  1:6342779/93‑1 (MQ=255)

            cccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTa                                                                              <  2:1938162/93‑1 (MQ=255)

             ccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTAt                                                                             >  2:6518730/1‑93 (MQ=255)

               ttATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCt                                                                           <  1:1874315/93‑1 (MQ=255)

                tATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTg                                                                          <  2:3230832/93‑1 (MQ=255)

                 aTGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGt                                                                         <  2:32903/93‑1 (MQ=255)

                  tGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGtt                                                                        >  1:6389500/1‑93 (MQ=255)

                  tGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGtt                                                                        >  1:6814585/1‑93 (MQ=255)

                    acacCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCt                                                                      <  2:1309847/93‑1 (MQ=255)

                      accacGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTg                                                                    >  2:4069414/1‑93 (MQ=255)

                       ccacGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGc                                                                   >  1:3252854/1‑93 (MQ=255)

                       ccacGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGc                                                                   >  2:2131977/1‑93 (MQ=255)

                          cGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGa                                                                <  1:5520123/93‑1 (MQ=255)

                             cATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAata                                                             >  2:1136785/1‑93 (MQ=255)

                              aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAatat                                                            >  1:6926617/1‑93 (MQ=255)

                              aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAatat                                                            <  1:752780/93‑1 (MQ=255)

                              aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAatat                                                            <  2:4547090/93‑1 (MQ=255)

                              aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAatat                                                            >  1:559271/1‑93 (MQ=255)

                              aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAatat                                                            >  1:3607580/1‑93 (MQ=255)

                               ttCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATg                                                           <  1:4298685/93‑1 (MQ=255)

                               ttCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATg                                                           <  2:5997406/93‑1 (MQ=255)

                                tCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGc                                                          >  1:3720205/1‑93 (MQ=255)

                                tCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGc                                                          >  2:3482842/1‑93 (MQ=255)

                                tCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGc                                                          <  1:3326316/93‑1 (MQ=255)

                                tCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGc                                                          <  1:2638409/93‑1 (MQ=255)

                                  gTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGa                                                        <  2:3694375/93‑1 (MQ=255)

                                   tCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAg                                                       >  2:4397553/1‑93 (MQ=255)

                                   tCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAg                                                       >  1:3936468/1‑93 (MQ=255)

                                   tCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAg                                                       >  2:5183080/1‑93 (MQ=255)

                                   tCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAg                                                       <  1:4448589/93‑1 (MQ=255)

                                    ccAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGc                                                      >  1:1489415/1‑93 (MQ=255)

                                    ccAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGc                                                      >  1:2985958/1‑93 (MQ=255)

                                     cAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCa                                                     >  2:2837396/1‑93 (MQ=255)

                                     cAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCa                                                     >  1:2319334/1‑93 (MQ=255)

                                     cAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCa                                                     >  2:1395673/1‑93 (MQ=255)

                                       gCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAAc                                                   <  1:548413/93‑1 (MQ=255)

                                       gCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAAc                                                   >  1:3129294/1‑93 (MQ=255)

                                         tttCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGc                                                 >  1:3508857/1‑93 (MQ=255)

                                         tttCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGc                                                 >  2:3179972/1‑93 (MQ=255)

                                           tCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTg                                               >  2:6809356/1‑93 (MQ=255)

                                           tCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTg                                               >  1:672801/1‑93 (MQ=255)

                                           tCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTg                                               >  2:1643782/1‑93 (MQ=255)

                                             tATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGaa                                             >  2:3463020/1‑92 (MQ=255)

                                             tATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGAt                                             >  2:923855/1‑93 (MQ=255)

                                             tATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGAt                                             >  2:301077/1‑93 (MQ=255)

                                             tATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGAt                                             <  2:6944699/93‑1 (MQ=255)

                                               ttCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATcc                                           >  1:4219926/1‑93 (MQ=255)

                                                tCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATccc                                          <  2:4214121/93‑1 (MQ=255)

                                                    gAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCccacc                                      >  1:5648478/1‑93 (MQ=255)

                                                    gAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCccacc                                      >  1:35251/1‑93 (MQ=255)

                                                     aaGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCccacca                                     <  1:1076803/93‑1 (MQ=255)

                                                     aaGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCccacca                                     >  1:6099120/1‑93 (MQ=255)

                                                      aGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCAt                                    <  1:1242403/93‑1 (MQ=255)

                                                       gTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCAtt                                   >  1:2619287/1‑93 (MQ=255)

                                                        tGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTg                                  >  2:1711568/1‑93 (MQ=255)

                                                        tGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTg                                  >  1:7250679/1‑93 (MQ=255)

                                                         ggTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGc                                 <  2:4099242/93‑1 (MQ=255)

                                                         ggTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGc                                 <  2:3965879/93‑1 (MQ=255)

                                                         ggTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGc                                 >  1:276925/1‑93 (MQ=255)

                                                         ggTAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGc                                 <  1:5804357/93‑1 (MQ=255)

                                                           tAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCgg                               <  2:7423042/93‑1 (MQ=255)

                                                           tAAAAACGCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCgg                               >  2:594858/1‑93 (MQ=255)

                                                               aaCGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTAcc                           <  1:4625972/93‑1 (MQ=255)

                                                                  gCTGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCgt                        <  2:2529232/93‑1 (MQ=255)

                                                                    tGTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCgtgg                      >  1:2154152/1‑93 (MQ=255)

                                                                     gTTCGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCgtggt                     <  1:2813293/93‑1 (MQ=255)

                                                                      ttCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCgtggtg                    >  2:5237172/1‑93 (MQ=255)

                                                                        cGAAGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGtgtt                  <  1:4498034/93‑1 (MQ=255)

                                                                          aaGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGtgttgt                >  1:4085239/1‑93 (MQ=255)

                                                                           aGCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTgttgtt               >  2:6580313/1‑93 (MQ=255)

                                                                            gCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTa              <  2:1821785/93‑1 (MQ=255)

                                                                            gCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTa              <  2:58462/93‑1 (MQ=255)

                                                                            gCCATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTa              <  2:4634608/93‑1 (MQ=255)

                                                                             ccATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAg             >  1:526203/1‑93 (MQ=255)

                                                                             ccATTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAg             <  1:3984330/93‑1 (MQ=255)

                                                                               aTTATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAggg           >  1:1346987/1‑93 (MQ=255)

                                                                                ttATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGa          >  1:4950315/1‑93 (MQ=255)

                                                                                ttATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGa          >  2:6152305/1‑93 (MQ=255)

                                                                                ttATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGa          <  2:6293469/93‑1 (MQ=255)

                                                                                ttATGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGa          >  1:5027215/1‑93 (MQ=255)

                                                                                   tGCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGt       <  1:906329/93‑1 (MQ=255)

                                                                                    gCTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGtt      <  2:1066548/93‑1 (MQ=255)

                                                                                     cTGGTGCTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGttt     >  2:5353448/1‑93 (MQ=255)

                                                                                        gtgcTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGTTTGcc  >  2:78852/1‑93 (MQ=255)

GAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTGATGTATCTGTTCTTGCAGAATATGCGAGCAACGCTGATCCCCACCATTGCGGTACCCGTGGTGTTGTTAGGGACGTTTGCC  >  NC_000913/3413632‑3413812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`