Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,522,805 A→G Y774C (TAC→TGC)  mrcA → penicillin‑binding protein 1A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,522,8050AG84.3% 684.1 / 119.1 306Y774C (TAC→TGC) mrcApenicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (137/120);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-02

Only 100 of 306 total aligned reads displayed.

GTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGC  >  NC_000913/3522714‑3522894
                                                                                           |                                                                                         
 tgACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCg                                                                                         >  2:4073677/1‑93 (MQ=255)
  gACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGa                                                                                        >  1:6638325/1‑93 (MQ=255)
   aCCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGaa                                                                                       >  1:3841770/1‑93 (MQ=255)
    ccTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAg                                                                                      >  2:4037083/1‑93 (MQ=255)
     cTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAgg                                                                                     >  1:1759338/1‑93 (MQ=255)
      tCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                                                                                    <  1:3747479/93‑1 (MQ=255)
       cGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCg                                                                                   >  1:31996/1‑93 (MQ=255)
         gTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGt                                                                                 >  2:1323689/1‑93 (MQ=255)
           cTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGc                                                                               >  2:7047549/1‑93 (MQ=255)
           cTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGc                                                                               >  1:5149567/1‑93 (MQ=255)
             ggATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCa                                                                             >  1:5740130/1‑93 (MQ=255)
             ggATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCa                                                                             >  2:3130510/1‑93 (MQ=255)
              gATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCaa                                                                            >  2:6883960/1‑93 (MQ=255)
               aTTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAag                                                                           >  1:3582144/1‑93 (MQ=255)
                ttGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAaga                                                                          <  1:1837651/93‑1 (MQ=255)
                ttGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAaga                                                                          >  2:5607106/1‑93 (MQ=255)
                ttGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAaga                                                                          <  2:6634760/93‑1 (MQ=255)
                 tGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAagag                                                                         <  1:617538/93‑1 (MQ=255)
                  ggCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGt                                                                        <  2:6200533/93‑1 (MQ=255)
                   gCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTg                                                                       <  1:2433948/93‑1 (MQ=255)
                      ttGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGccc                                                                    <  2:3718145/93‑1 (MQ=255)
                      ttGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGccc                                                                    >  1:3483662/1‑93 (MQ=255)
                          tgatCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGcc                                                                <  2:6721241/93‑1 (MQ=255)
                           gatCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCt                                                               <  2:5744746/93‑1 (MQ=255)
                            atCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTg                                                              >  1:2399288/1‑93 (MQ=255)
                             tCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGc                                                             >  2:2264232/1‑93 (MQ=255)
                             tCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCCCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGc                                                             >  2:2376358/1‑93 (MQ=255)
                              cACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCa                                                            >  1:6884121/1‑93 (MQ=255)
                                  gtcgtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATggg                                                        >  1:5847205/1‑93 (MQ=255)
                                   tcgtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGa                                                       >  2:1574981/1‑93 (MQ=255)
                                   tcgtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGa                                                       >  2:5390007/1‑93 (MQ=255)
                                   tcgtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGa                                                       <  2:492231/93‑1 (MQ=255)
                                    nntAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGAc                                                      <  1:2571683/91‑1 (MQ=255)
                                    cgtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGAc                                                      <  2:6372702/93‑1 (MQ=255)
                                     gtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACg                                                     <  2:3809597/93‑1 (MQ=255)
                                     gtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACg                                                     >  1:2967144/1‑93 (MQ=255)
                                     gtAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACg                                                     >  2:5227894/1‑93 (MQ=255)
                                      tAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGc                                                    <  2:720933/93‑1 (MQ=255)
                                      tAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGc                                                    >  2:1732675/1‑93 (MQ=255)
                                       aaTCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCt                                                   >  1:5031336/1‑93 (MQ=255)
                                         tctcGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTta                                                 <  2:4993190/93‑1 (MQ=255)
                                         tctcGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTta                                                 <  1:1277539/93‑1 (MQ=255)
                                         tctcGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTta                                                 <  2:1103736/93‑1 (MQ=255)
                                         tctcGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTta                                                 >  1:4780426/1‑93 (MQ=255)
                                          ctcGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTtat                                                <  1:3302714/93‑1 (MQ=255)
                                             ggTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATg                                             <  1:2855312/93‑1 (MQ=255)
                                              gTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGa                                            <  1:4253735/93‑1 (MQ=255)
                                              gTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGa                                            >  1:7100122/1‑93 (MQ=255)
                                              gTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGa                                            >  1:7349287/1‑93 (MQ=255)
                                               tCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGaa                                           >  2:2022753/1‑93 (MQ=255)
                                               tCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGaa                                           >  2:281218/1‑93 (MQ=255)
                                                cATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGaaa                                          >  1:849087/1‑93 (MQ=255)
                                                 aTACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAg                                         >  2:7419356/1‑93 (MQ=255)
                                                 aTACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAg                                         >  2:1605520/1‑93 (MQ=255)
                                                  tagaaCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGc                                        >  2:3940699/4‑93 (MQ=255)
                                                  tACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGc                                        <  1:3093805/93‑1 (MQ=255)
                                                    cAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCg                                      <  2:1018588/93‑1 (MQ=255)
                                                     aaCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGt                                     <  2:2105463/93‑1 (MQ=255)
                                                     aaCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGt                                     <  2:4757499/93‑1 (MQ=255)
                                                      aCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGtt                                    >  2:1453779/1‑93 (MQ=255)
                                                      aCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGtt                                    >  1:5422781/1‑93 (MQ=255)
                                                        ggCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCCTGGGACGCTTATATGAAAGCCGTTCt                                  >  1:6394773/1‑93 (MQ=255)
                                                        ggCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCt                                  <  1:7209086/93‑1 (MQ=255)
                                                        ggCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCt                                  <  1:4646873/93‑1 (MQ=255)
                                                         gCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCtt                                 >  1:6959023/1‑93 (MQ=255)
                                                         gCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCtt                                 <  2:5072375/93‑1 (MQ=255)
                                                         gCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCtt                                 >  2:6082225/1‑93 (MQ=255)
                                                          cTTCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTg                                >  2:4528421/1‑93 (MQ=255)
                                                           ttCCGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGa                               <  1:1652409/93‑1 (MQ=255)
                                                              cGGAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAgg                            >  2:591186/1‑93 (MQ=255)
                                                                gAGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGgtg                          <  1:1037132/93‑1 (MQ=255)
                                                                 aGCGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGgtgt                         <  1:1143481/93‑1 (MQ=255)
                                                                   cGATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGc                       <  2:3380111/93‑1 (MQ=255)
                                                                   cGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGc                       <  2:5983541/93‑1 (MQ=255)
                                                                    gATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGcc                      >  1:6244042/1‑93 (MQ=255)
                                                                    gATTAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGcc                      <  2:5552828/93‑1 (MQ=255)
                                                                      ttAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCgg                    <  2:4355854/93‑1 (MQ=255)
                                                                       tAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGa                   <  1:2130176/93‑1 (MQ=255)
                                                                       tAAAGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGa                   <  1:3964797/93‑1 (MQ=255)
                                                                        aaaGATCAGATCTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGag                  <  1:7032232/93‑1 (MQ=255)
                                                                         aaGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGagc                 >  1:232897/1‑93 (MQ=255)
                                                                          agatcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGagca                >  1:5520628/1‑93 (MQ=255)
                                                                            atcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGagcagc              <  1:1440959/93‑1 (MQ=255)
                                                                             tcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGcc             <  1:4400678/93‑1 (MQ=255)
                                                                             tcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGcc             >  2:4017037/1‑93 (MQ=255)
                                                                             tcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGcc             <  1:4590642/93‑1 (MQ=255)
                                                                             tcagatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGcc             >  1:6782054/1‑93 (MQ=255)
                                                                               agatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGc           <  1:3555060/93‑1 (MQ=255)
                                                                                gatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCt          <  2:5686816/93‑1 (MQ=255)
                                                                                gatcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCt          >  2:2337124/1‑93 (MQ=255)
                                                                                gatcTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCt          >  1:900765/1‑93 (MQ=255)
                                                                                 atcTCAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTg         <  2:320789/93‑1 (MQ=255)
                                                                                  tctcAGGTTGCGAAGGCGGTGCTAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGa        <  2:4616882/93‑1 (MQ=255)
                                                                                  tctcAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGa        <  2:3320373/93‑1 (MQ=255)
                                                                                  tctcAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGa        >  1:4886612/1‑93 (MQ=255)
                                                                                  tctcAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGa        <  1:4073867/93‑1 (MQ=255)
                                                                                  tctcAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGa        <  2:792037/93‑1 (MQ=255)
                                                                                   ctcAGGTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGAc       >  1:6079785/1‑93 (MQ=255)
                                                                                       ggTTGCGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGAcgccg   >  2:2512247/1‑93 (MQ=255)
                                                                                        gTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGAcgccgc  >  1:4966857/1‑93 (MQ=255)
                                                                                           |                                                                                         
GTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCCAGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGC  >  NC_000913/3522714‑3522894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑