BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	394,595	T→C	D306G (GAT→GGT)	yaiH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	394,595	0	T	C	86.9%	663.1 / 86.2	274	D306G (GAT→GGT)	yaiH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (24/12); new base C (115/123); total (139/135)
Fisher's exact test for biased strand distribution p-value = 4.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 280 total aligned reads displayed.

AGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGC  >  NC_000913/394503‑394687

   tAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCg                                                                                           >  2:5000667/1‑93 (MQ=255)

   tAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCT‑AACCAAGCGCACCCGc                                                                                          >  2:1332441/1‑93 (MQ=255)

    aaTGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGc                                                                                          <  2:924771/93‑1 (MQ=255)

    aaTGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGc                                                                                          >  1:5641073/1‑93 (MQ=255)

    aaTGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGc                                                                                          <  2:3770275/93‑1 (MQ=255)

       gAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCttt                                                                                       <  1:5300142/93‑1 (MQ=255)

          aCCGCCACCCCCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGcc                                                                                    <  1:4717462/93‑1 (MQ=255)

            cGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCgg                                                                                  <  1:2813567/93‑1 (MQ=255)

              ccaccaCCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCgggg                                                                                <  1:6479618/93‑1 (MQ=255)

              ccaccaCCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCgggg                                                                                >  1:492330/1‑93 (MQ=255)

               caccacCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGa                                                                               <  2:1173465/93‑1 (MQ=255)

                accaccGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGAc                                                                              >  1:1315453/1‑93 (MQ=255)

                accaccGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGAc                                                                              >  1:5489574/1‑93 (MQ=255)

                 ccaccGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACa                                                                             <  1:5397144/93‑1 (MQ=255)

                  caccGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCGCCCGCTTTGCCGGGGACAt                                                                            >  2:5746213/1‑93 (MQ=255)

                   accGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATc                                                                           >  1:3858798/1‑93 (MQ=255)

                   accGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATc                                                                           <  1:534193/93‑1 (MQ=255)

                   accGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATc                                                                           >  1:831847/1‑93 (MQ=255)

                   accGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATc                                                                           <  2:210307/93‑1 (MQ=255)

                     cGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCa                                                                         <  2:4640324/93‑1 (MQ=255)

                     cGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCa                                                                         <  2:6625333/93‑1 (MQ=255)

                     cGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCa                                                                         >  2:2778506/1‑93 (MQ=255)

                     cGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCa                                                                         <  1:5042362/93‑1 (MQ=255)

                     cGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCa                                                                         <  1:7069982/93‑1 (MQ=255)

                      gCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCAt                                                                        >  2:6104744/1‑93 (MQ=255)

                      gCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCAt                                                                        >  1:4196162/1‑93 (MQ=255)

                      gCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCAt                                                                        <  2:1791029/93‑1 (MQ=255)

                      gCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCAt                                                                        <  1:6891575/93‑1 (MQ=255)

                       ccTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATg                                                                       >  2:296637/1‑93 (MQ=255)

                         tGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGcc                                                                     >  1:6311539/1‑93 (MQ=255)

                           tcttctGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCaa                                                                   >  1:6168152/1‑93 (MQ=255)

                              tctGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCa                                                                >  1:2411542/1‑93 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              <  2:3640634/93‑1 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              <  2:2688626/93‑1 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              >  2:7420410/1‑93 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              >  2:3157844/1‑93 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              >  2:4380971/1‑93 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              >  2:7294167/1‑93 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              <  2:1074252/93‑1 (MQ=255)

                                tGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACt                                                              >  1:2324281/1‑93 (MQ=255)

                                     aatCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGt                                                         <  2:5164870/93‑1 (MQ=255)

                                      atCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTa                                                        <  1:7399059/93‑1 (MQ=255)

                                       tCCCCGGACGACCCTCTTTCGGGGGCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTaa                                                       <  1:7206408/93‑1 (MQ=255)

                                       tCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTaa                                                       >  1:461113/1‑93 (MQ=255)

                                        ccccGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTaaa                                                      <  2:6372482/93‑1 (MQ=255)

                                        ccccGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTaaa                                                      <  1:248273/93‑1 (MQ=255)

                                         cccGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAAc                                                     <  2:5016876/93‑1 (MQ=255)

                                            ggACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgc                                                  <  1:7441752/93‑1 (MQ=255)

                                             gacgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcg                                                 >  1:4772417/1‑93 (MQ=255)

                                             gacgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcg                                                 >  1:1982041/1‑93 (MQ=255)

                                              acgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcgc                                                >  2:4065993/1‑93 (MQ=255)

                                              acgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcgc                                                <  1:2925398/93‑1 (MQ=255)

                                              acgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcgc                                                <  2:1954176/93‑1 (MQ=255)

                                                gacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcgcgc                                              >  2:5466841/1‑93 (MQ=255)

                                                  cccTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTgcgcgcgc                                            >  1:3331268/1‑93 (MQ=255)

                                                    ctctTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTg                                          >  2:146609/1‑93 (MQ=255)

                                                    ctctTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTg                                          >  1:3601747/1‑93 (MQ=255)

                                                      ctTTCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGAt                                        >  1:5843045/1‑93 (MQ=255)

                                                       tttCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATa                                       >  2:7088202/1‑93 (MQ=255)

                                                       tttCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATa                                       >  1:3657811/1‑93 (MQ=255)

                                                       tttCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATa                                       >  2:5599703/1‑93 (MQ=255)

                                                       tttCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATa                                       >  1:1932279/1‑93 (MQ=255)

                                                         tCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATaaa                                     <  1:5207361/93‑1 (MQ=255)

                                                         tCGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATaaa                                     >  1:3806179/1‑93 (MQ=255)

                                                          cGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAAt                                    <  1:3465637/93‑1 (MQ=255)

                                                          cGGGGCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAAt                                    <  2:3914535/93‑1 (MQ=255)

                                                           ggggCCATATATACCCAACCTAAACCAAGCGCACACGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATc                                   <  2:5531243/93‑1 (MQ=255)

                                                             ggCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCaa                                 <  1:4820013/93‑1 (MQ=255)

                                                             ggCCATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCaa                                 <  2:6482572/93‑1 (MQ=255)

                                                                cATATATACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGt                              <  2:674881/93‑1 (MQ=255)

                                                                 atatatACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTc                             <  2:21860/93‑1 (MQ=255)

                                                                 atatatACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTc                             >  2:3405988/1‑93 (MQ=255)

                                                                 atatatACCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTc                             <  2:1809121/93‑1 (MQ=255)

                                                                  tatataCCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCt                            >  1:2203747/1‑93 (MQ=255)

                                                                    tataCCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGc                          >  1:3983900/1‑93 (MQ=255)

                                                                     ataCCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCa                         >  1:1183353/1‑93 (MQ=255)

                                                                     ataCCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCa                         <  2:4213319/93‑1 (MQ=255)

                                                                     ataCCCAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCa                         >  1:50751/1‑93 (MQ=255)

                                                                         ccAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCg                     >  1:7458225/1‑93 (MQ=255)

                                                                         ccAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCg                     >  2:6340461/1‑93 (MQ=255)

                                                                          cAACCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGa                    >  2:3353007/1‑93 (MQ=255)

                                                                           aaCCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGAt                   <  1:1019827/93‑1 (MQ=255)

                                                                            aCCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATc                  >  2:2057929/1‑93 (MQ=255)

                                                                            aCCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATc                  >  1:3749531/1‑93 (MQ=255)

                                                                            aCCTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATc                  >  2:6914037/1‑93 (MQ=255)

                                                                             ccTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCt                 <  1:7146188/93‑1 (MQ=255)

                                                                              cTAAACCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTg                <  2:4165410/93‑1 (MQ=255)

                                                                                aaaCCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGcc              <  2:2646023/93‑1 (MQ=255)

                                                                                aaaCCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGcc              >  2:6053326/1‑93 (MQ=255)

                                                                                aaaCCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGcc              <  1:1750232/93‑1 (MQ=255)

                                                                                 aaCCAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCt             >  1:2572694/1‑93 (MQ=255)

                                                                                    cAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGt          <  1:5751269/93‑1 (MQ=255)

                                                                                    cAAGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGt          <  1:3229026/93‑1 (MQ=255)

                                                                                      aGCGCACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTa        <  2:2371838/93‑1 (MQ=255)

                                                                                       gcgcACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTAc       <  2:1687176/93‑1 (MQ=255)

                                                                                       gcgcACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTAc       <  1:5956482/93‑1 (MQ=255)

                                                                                       gcgcACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTAc       <  1:6781885/93‑1 (MQ=255)

                                                                                       gcgcACCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTAc       >  1:1462366/1‑93 (MQ=255)

                                                                                            tCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTgcgc  >  2:596311/1‑93 (MQ=255)

                                                                                            tCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTgcgc  >  1:2864539/1‑93 (MQ=255)

AGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGC  >  NC_000913/394503‑394687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`