BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	403,861	T→C	V312A (GTG→GCG)	yaiC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	403,861	0	T	C	87.3%	697.6 / 71.2	276	V312A (GTG→GCG)	yaiC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/17); new base C (112/129); total (130/146)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GCGATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAA  >  NC_000913/403771‑403950

gCGATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGgcgc                                                                                         >  2:7130154/1‑93 (MQ=255)

 cGATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCa                                                                                        <  1:5500228/93‑1 (MQ=255)

  gATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCAt                                                                                       >  2:2575155/1‑93 (MQ=255)

  gATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCAt                                                                                       >  1:1754097/1‑93 (MQ=255)

   aTGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATg                                                                                      >  2:3271530/1‑93 (MQ=255)

   aTGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATg                                                                                      >  1:5865806/1‑93 (MQ=255)

    tgtgATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGa                                                                                     >  2:3797689/1‑93 (MQ=255)

    tgtgATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGa                                                                                     >  1:4372501/1‑93 (MQ=255)

     gtgATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGaa                                                                                    >  2:6416390/1‑93 (MQ=255)

     gtgATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGaa                                                                                    >  2:6575882/1‑93 (MQ=255)

      tgATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAg                                                                                   <  2:610049/93‑1 (MQ=255)

       gATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAgg                                                                                  <  2:4450091/93‑1 (MQ=255)

       gATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAgg                                                                                  <  2:843239/93‑1 (MQ=255)

       gATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAgg                                                                                  >  2:6703845/1‑93 (MQ=255)

        aTTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGGTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAggg                                                                                 >  1:4670162/1‑93 (MQ=255)

        aTTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAggg                                                                                 <  1:4877079/93‑1 (MQ=255)

        aTTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAggg                                                                                 <  2:3709954/93‑1 (MQ=255)

         ttGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGc                                                                                >  2:4977380/1‑93 (MQ=255)

         ttGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGc                                                                                >  1:4974117/1‑93 (MQ=255)

             tCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTaaa                                                                            >  1:3143190/1‑93 (MQ=255)

             tCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTaaa                                                                            <  2:1036290/93‑1 (MQ=255)

             tCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTaaa                                                                            >  2:3556483/1‑93 (MQ=255)

              cGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAAt                                                                           <  1:5608663/93‑1 (MQ=255)

               ggTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATa                                                                          >  1:2648451/1‑93 (MQ=255)

                 tttGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACa                                                                        >  2:1851901/1‑93 (MQ=255)

                 tttGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACa                                                                        >  1:6149877/1‑93 (MQ=255)

                   tGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACAtt                                                                      <  2:2725527/93‑1 (MQ=255)

                   tGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACAtt                                                                      <  1:7163344/93‑1 (MQ=255)

                    ggcggcGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTa                                                                     <  2:3492916/93‑1 (MQ=255)

                     gcggcgATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTAc                                                                    <  2:3392639/93‑1 (MQ=255)

                        gcgATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGtt                                                                 <  2:4265445/93‑1 (MQ=255)

                        gcgATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGtt                                                                 >  2:7379083/1‑93 (MQ=255)

                         cgATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGttt                                                                >  2:6240294/1‑93 (MQ=255)

                         cgATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGttt                                                                <  2:252138/93‑1 (MQ=255)

                          gATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTg                                                               >  2:6733480/1‑93 (MQ=255)

                           aTGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGc                                                              >  1:1049823/1‑93 (MQ=255)

                            tGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGcc                                                             <  1:5354443/93‑1 (MQ=255)

                                 ttGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATa                                                        <  2:6920305/93‑1 (MQ=255)

                                 ttGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATa                                                        <  2:6756423/93‑1 (MQ=255)

                                 ttGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATa                                                        <  2:5012323/93‑1 (MQ=255)

                                  tGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATAc                                                       <  1:1428076/93‑1 (MQ=255)

                                  tGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATAc                                                       <  1:4241226/93‑1 (MQ=255)

                                   gCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACg                                                      >  1:237613/1‑93 (MQ=255)

                                   gCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACg                                                      <  1:1542354/93‑1 (MQ=255)

                                    cAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGc                                                     <  1:4113345/93‑1 (MQ=255)

                                     aGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGcc                                                    <  2:5357653/93‑1 (MQ=255)

                                      gTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCca                                                   <  2:2163737/93‑1 (MQ=255)

                                       tAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCcac                                                  >  2:1622537/1‑93 (MQ=255)

                                        aaTCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCcaca                                                 >  2:4143279/1‑93 (MQ=255)

                                        aaTCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCcaca                                                 <  1:3778914/93‑1 (MQ=255)

                                        aaTCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCcaca                                                 >  1:2727270/1‑93 (MQ=255)

                                        aaTCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCcaca                                                 <  2:5578515/93‑1 (MQ=255)

                                             tGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTaa                                            <  2:1721484/93‑1 (MQ=255)

                                             tGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTaa                                            >  2:7478767/1‑93 (MQ=255)

                                             tGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTaa                                            >  1:7211334/1‑93 (MQ=255)

                                                ccGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACtt                                         >  1:1603910/1‑93 (MQ=255)

                                                   gTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTAc                                      >  2:3986202/1‑93 (MQ=255)

                                                   gTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTAc                                      <  2:6837691/93‑1 (MQ=255)

                                                      cGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGa                                   <  1:6508409/93‑1 (MQ=255)

                                                       gCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGAt                                  <  1:3920216/93‑1 (MQ=255)

                                                        ccAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGAtt                                 >  1:7521718/1‑93 (MQ=255)

                                                         cAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTa                                <  1:6128453/93‑1 (MQ=255)

                                                         cAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTa                                <  2:2090137/93‑1 (MQ=255)

                                                         cAGCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTa                                <  2:1906187/93‑1 (MQ=255)

                                                           gCTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAgt                              >  1:4440244/1‑93 (MQ=255)

                                                            cTGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAgtg                             <  2:3035160/93‑1 (MQ=255)

                                                             tGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAgtgt                            >  2:5831631/1‑93 (MQ=255)

                                                             tGAGAGCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAgtgt                            <  1:2156385/93‑1 (MQ=255)

                                                             tGAGAGCGCCATCACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAgtgt                            <  2:464191/93‑1 (MQ=255)

                                                               agagCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTgg                          >  1:7057800/1‑93 (MQ=255)

                                                                gagCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTggg                         >  1:5697615/1‑93 (MQ=255)

                                                                gagCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTggg                         <  1:5738982/93‑1 (MQ=255)

                                                                 agCGCCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTgggg                        <  2:1276124/93‑1 (MQ=255)

                                                                  gcgcCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTggggg                       >  2:358505/1‑93 (MQ=255)

                                                                  gcgcCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTggggg                       >  1:6564448/1‑93 (MQ=255)

                                                                   cgcCATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGt                      <  2:1397056/93‑1 (MQ=255)

                                                                   cgcCATTACCGCCATGTTACAGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGt                      <  2:6499969/93‑1 (MQ=255)

                                                                     ccATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTg                    <  2:3875886/93‑1 (MQ=255)

                                                                     ccATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTg                    >  1:3182301/1‑93 (MQ=255)

                                                                     ccATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTg                    >  2:4717124/1‑93 (MQ=255)

                                                                      cATTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgc                   >  1:195871/1‑93 (MQ=255)

                                                                       aTTACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgcg                  >  1:211962/1‑93 (MQ=255)

                                                                        ttACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgcgc                 >  1:2479242/1‑93 (MQ=255)

                                                                        ttACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgcgc                 >  2:2311496/1‑93 (MQ=255)

                                                                        ttACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgcgc                 <  2:5473645/93‑1 (MQ=255)

                                                                        ttACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTgcgc                 >  2:5134858/1‑93 (MQ=255)

                                                                         tACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGcgcc                >  1:3284312/1‑93 (MQ=255)

                                                                         tACCGCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGcgcc                >  2:7186506/1‑93 (MQ=255)

                                                                             gCCATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTg            >  1:1124434/1‑93 (MQ=255)

                                                                              ccATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGa           >  1:6764968/1‑93 (MQ=255)

                                                                               cATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGaa          >  2:4355263/1‑93 (MQ=255)

                                                                               cATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGaa          <  1:7356194/93‑1 (MQ=255)

                                                                               cATGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGaa          <  2:2443857/93‑1 (MQ=255)

                                                                                aTGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAAc         >  1:2849047/1‑93 (MQ=255)

                                                                                aTGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAAc         <  1:6974217/93‑1 (MQ=255)

                                                                                aTGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAAc         <  1:1698544/93‑1 (MQ=255)

                                                                                aTGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAAc         >  1:1291977/1‑93 (MQ=255)

                                                                                aTGTTACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAACACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAAc         >  2:4675711/1‑93 (MQ=255)

                                                                                    tACGGGCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCcac     <  2:2987674/93‑1 (MQ=255)

                                                                                       gggCGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACaaa  <  1:68979/93‑1 (MQ=255)

GCGATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACGCCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAA  >  NC_000913/403771‑403950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`