Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,765,203	A→T	Q119L (CAG→CTG)	yibJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,765,203	0	A	T	100.0%	178.8 / NA	62	Q119L (CAG→CTG)	yibJ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/34);  total (28/34)

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292                    |                                                                                          cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATTCGTTGAATCCg                  <  1:6190331/93‑1 (MQ=11) cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  <  2:4669950/93‑1 (MQ=11) cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  <  2:3600433/93‑1 (MQ=255) cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  >  2:5542218/1‑93 (MQ=11) cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  <  1:6190330/93‑1 (MQ=11)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  1:6031325/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  1:2279092/1‑93 (MQ=11)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:7388879/93‑1 (MQ=11)  gCTATTATGACCCGCTGCTGAGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  2:3848920/1‑93 (MQ=11)   cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                <  1:1893768/93‑1 (MQ=255)   cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                <  1:1793720/93‑1 (MQ=255)   cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                >  1:6053360/1‑93 (MQ=255)   cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                >  1:7408718/1‑93 (MQ=11)   cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                <  2:3646459/93‑1 (MQ=11)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:3116323/1‑93 (MQ=11)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:1841865/1‑93 (MQ=11)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               <  1:515827/93‑1 (MQ=255)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               <  1:5373614/93‑1 (MQ=255)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              >  2:2175079/1‑93 (MQ=11)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              <  2:2473433/93‑1 (MQ=255)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              >  2:4005409/1‑93 (MQ=11)      ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:6552153/1‑93 (MQ=11)      ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:5128974/1‑93 (MQ=11)       tatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:7123895/1‑93 (MQ=255)       tatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  2:6471833/1‑93 (MQ=14)       tatGACCCGCTGCTGGGCCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  2:4019234/1‑93 (MQ=14)        atGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  1:6107289/1‑93 (MQ=14)        atGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  2:817952/1‑93 (MQ=14)        atGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  1:2565487/1‑93 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  2:7017575/1‑93 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  2:6810002/1‑93 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  1:1388918/1‑93 (MQ=14)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:269937/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:5592055/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  2:3999048/1‑93 (MQ=14)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:214392/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:6250145/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:7332181/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:63793/93‑1 (MQ=255)          nncccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         <  2:1692195/91‑1 (MQ=14)          gACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGGTGGAATTTTTATCAGTATCCGTTGAATCTGGTCATCAAt         >  2:6672703/1‑93 (MQ=11)          gACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         <  2:5679361/93‑1 (MQ=255)           aCCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATg        >  1:3678406/1‑93 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  2:4420312/93‑1 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  1:6064582/93‑1 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  2:5993345/93‑1 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  1:503658/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:935215/1‑93 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:7343578/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:5225013/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  2:4441839/93‑1 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     >  1:3105227/1‑93 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  2:4850651/93‑1 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     >  1:1283297/1‑93 (MQ=17)              cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     >  2:4101477/1‑93 (MQ=17)              cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:2975663/93‑1 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  2:6823404/1‑93 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  1:2259108/93‑1 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  1:2735532/1‑93 (MQ=17)               gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  2:2626870/1‑93 (MQ=17)               gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  1:7345484/1‑93 (MQ=17)               gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  1:6981792/93‑1 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  1:6125868/93‑1 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   >  1:3505997/1‑93 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   >  2:5385703/1‑93 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  2:447573/93‑1 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  1:1687083/93‑1 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  1:117759/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  1:5170924/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:611210/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:2438878/1‑93 (MQ=17)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:268446/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:3031367/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:7413496/93‑1 (MQ=255)                    |                                                                                          CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑