BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,778,301	T→C	V283A (GTA→GCA)	lldD →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,778,301	0	T	C	85.9%	741.5 / 99.3	313	V283A (GTA→GCA)	lldD	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (21/23); new base C (130/139); total (151/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 314 total aligned reads displayed.

ATCCTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTAACGGGCT  >  NC_000913/3778210‑3778391

 tCCTCGATCCGGAAGATGCGCGCAATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCAc                                                                                          <  2:5827875/93‑1 (MQ=255)

  ccTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTAct                                                                                         >  1:6191359/1‑93 (MQ=255)

  ccTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCAct                                                                                         >  2:602581/1‑93 (MQ=255)

   cTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCCGGACGGTGTActc                                                                                        <  1:2140365/93‑1 (MQ=255)

      gATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCActctct                                                                                     <  2:5506348/93‑1 (MQ=255)

      gATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCActctct                                                                                     <  1:2719712/93‑1 (MQ=255)

      gATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGAGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCActctct                                                                                     >  1:2650059/1‑93 (MQ=255)

       aTCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCtt                                                                                    >  1:4590862/1‑93 (MQ=255)

        tCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTc                                                                                   <  2:3588038/93‑1 (MQ=255)

           ggAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGc                                                                                >  2:346908/1‑93 (MQ=255)

            gAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGcc                                                                               >  2:1337020/1‑93 (MQ=255)

                 tGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGc                                                                          >  1:3935865/1‑93 (MQ=255)

                 tGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGc                                                                          >  2:4833082/1‑93 (MQ=255)

                      gcgATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgc                                                                     <  1:202706/93‑1 (MQ=255)

                      gcgATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgc                                                                     >  2:6753202/1‑93 (MQ=255)

                      gcgATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgc                                                                     <  2:4975837/93‑1 (MQ=255)

                       cgATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcc                                                                    <  2:2430273/93‑1 (MQ=255)

                        gATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcct                                                                   >  2:1608/1‑93 (MQ=255)

                        gATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcct                                                                   >  1:6105768/1‑93 (MQ=255)

                        gATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcct                                                                   <  2:2521364/93‑1 (MQ=255)

                        gATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcct                                                                   >  1:3344395/1‑93 (MQ=255)

                          tGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcctgc                                                                 <  2:5258364/93‑1 (MQ=255)

                          tGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCActgcctgc                                                                 >  2:1474741/1‑93 (MQ=255)

                           gCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCt                                                                >  2:4313580/1‑93 (MQ=255)

                           gCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCt                                                                >  1:4089984/1‑93 (MQ=255)

                           gCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCt                                                                >  1:4312105/1‑93 (MQ=255)

                           gCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCt                                                                >  1:6897289/1‑93 (MQ=255)

                           gCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCt                                                                >  1:5459041/1‑93 (MQ=255)

                            cAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTa                                                               >  2:6230601/1‑93 (MQ=255)

                            cAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTa                                                               <  1:1693056/93‑1 (MQ=255)

                             aGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTAt                                                              <  1:2483837/93‑1 (MQ=255)

                             aGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTAt                                                              >  1:1351848/1‑93 (MQ=255)

                              gTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTAtt                                                             <  2:3171118/93‑1 (MQ=255)

                              gTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTAtt                                                             <  1:1144678/93‑1 (MQ=255)

                              gTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTAtt                                                             <  1:1113332/93‑1 (MQ=255)

                              gTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTAnt                                                             >  2:7431536/1‑93 (MQ=255)

                               tACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTg                                                            >  1:5085228/1‑93 (MQ=255)

                               tACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTg                                                            >  1:2583726/1‑93 (MQ=255)

                                  gTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAg                                                         >  2:6336266/1‑93 (MQ=255)

                                  gTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAg                                                         <  2:1993311/93‑1 (MQ=255)

                                   ttttGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGa                                                        <  2:5192693/93‑1 (MQ=255)

                                   ttttGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGa                                                        >  1:5730054/1‑93 (MQ=255)

                                   ttttGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGa                                                        <  2:5323177/93‑1 (MQ=255)

                                     ttGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATg                                                      <  1:70441/93‑1 (MQ=255)

                                         tGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGt                                                  <  1:5368544/93‑1 (MQ=255)

                                           cTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGa                                                <  2:1371310/93‑1 (MQ=255)

                                            tGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGaa                                               >  2:2566520/1‑93 (MQ=255)

                                            tGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGaa                                               >  1:2783267/1‑93 (MQ=255)

                                             gATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGaaa                                              <  1:1494945/93‑1 (MQ=255)

                                              aTGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAg                                             <  1:3236057/93‑1 (MQ=255)

                                              aTGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAg                                             <  1:7499360/93‑1 (MQ=255)

                                               tGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAgg                                            >  2:6496814/1‑93 (MQ=255)

                                                  aaTTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGa                                         <  1:4906470/93‑1 (MQ=255)

                                                   aTTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGat                                        >  2:411054/1‑93 (MQ=255)

                                                    ttGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGata                                       <  1:5886445/93‑1 (MQ=255)

                                                    ttGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGata                                       >  2:7057474/1‑93 (MQ=255)

                                                    ttGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGata                                       <  1:1748622/93‑1 (MQ=255)

                                                    ttGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGata                                       <  1:715189/93‑1 (MQ=255)

                                                     tgtgGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGatat                                      >  2:6512399/1‑93 (MQ=255)

                                                      gtgGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGAtata                                     <  1:6724342/93‑1 (MQ=255)

                                                      gtgGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGAtata                                     >  1:3804628/1‑93 (MQ=255)

                                                       tgGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAg                                    <  2:3969938/93‑1 (MQ=255)

                                                        ggTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGc                                   <  2:3786534/93‑1 (MQ=255)

                                                        ggTGTCTAACGACGGTGGCCGCCAGCTGGACGGTGCGCCCTCTCCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGc                                   <  2:6861925/93‑1 (MQ=255)

                                                         gTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGcc                                  >  1:6566368/1‑93 (MQ=255)

                                                          tttCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCa                                 >  1:5958828/1‑93 (MQ=255)

                                                           ttCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCAt                                <  1:934629/93‑1 (MQ=255)

                                                           ttCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCAt                                <  1:624209/93‑1 (MQ=255)

                                                            tCTAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCAtt                               >  2:4160056/1‑93 (MQ=255)

                                                              tAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCt                             >  2:2165039/1‑93 (MQ=255)

                                                              tAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCt                             <  1:1779039/93‑1 (MQ=255)

                                                              tAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCt                             >  1:4225028/1‑93 (MQ=255)

                                                              tAACCACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCt                             <  1:5981642/93‑1 (MQ=255)

                                                                 ccACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGc                          <  2:45540/93‑1 (MQ=255)

                                                                 ccACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGc                          >  1:4021079/1‑93 (MQ=255)

                                                                 ccACGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGc                          <  2:1278069/93‑1 (MQ=255)

                                                                   aCGGTGGCCGCCGGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCgg                        >  1:4151583/1‑93 (MQ=255)

                                                                    cGGTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGa                       >  2:6952406/1‑93 (MQ=255)

                                                                     ggTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGAt                      <  1:5903294/93‑1 (MQ=255)

                                                                     ggTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGAt                      >  2:4890976/1‑93 (MQ=255)

                                                                     ggTGGCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGAt                      >  2:2879971/1‑93 (MQ=255)

                                                                      gTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATa                     >  2:1050162/1‑93 (MQ=255)

                                                                        ggCCGCCAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGc                   <  1:3038186/93‑1 (MQ=255)

                                                                            gccAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGaa               >  1:3693676/1‑93 (MQ=255)

                                                                            gccAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGaa               <  2:3254101/93‑1 (MQ=255)

                                                                            gccAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGaa               <  1:4740547/93‑1 (MQ=255)

                                                                            gccAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGaa               <  1:5242474/93‑1 (MQ=255)

                                                                              cAGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAAtt             >  2:2256211/1‑93 (MQ=255)

                                                                               aGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTc            >  1:6848579/1‑93 (MQ=255)

                                                                               aGCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTc            >  2:1573294/1‑93 (MQ=255)

                                                                                gCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCg           <  1:2607818/93‑1 (MQ=255)

                                                                                gCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCg           >  1:3625997/1‑93 (MQ=255)

                                                                                gCTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCg           >  1:7345716/1‑93 (MQ=255)

                                                                                 cTGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGt          <  1:5547402/93‑1 (MQ=255)

                                                                                  tGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTa         >  2:1809893/1‑93 (MQ=255)

                                                                                  tGGACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTa         >  2:5655407/1‑93 (MQ=255)

                                                                                    gACGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTAAc       <  2:6076301/93‑1 (MQ=255)

                                                                                     aCGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTAACg      <  2:2981944/93‑1 (MQ=255)

                                                                                     aCGGTGCACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTAACg      <  2:3068661/93‑1 (MQ=255)

                                                                                         tgtACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCGGGCGGATAGCGGAATTCGTAACGGGCt  <  1:837107/93‑1 (MQ=255)

ATCCTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGCTGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCCCGTGCACTGCCTGCTATTGCAGATGCGGTGAAAGGTGATATAGCCATTCTGGCGGATAGCGGAATTCGTAACGGGCT  >  NC_000913/3778210‑3778391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`