BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,659	G→A	R173H (CGT→CAT)	spoU →	putative RNA methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,659	0	G	A	75.4%	650.9 / 182.1	317	R173H (CGT→CAT)	spoU	putative RNA methylase
Reads supporting (aligned to +/- strand): ref base G (42/36); new base A (125/114); total (167/150)
Fisher's exact test for biased strand distribution p-value = 8.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 319 total aligned reads displayed.

CGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCC  >  NC_000913/3822567‑3822751

  gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATg                                                                                            >  2:713727/1‑93 (MQ=255)

  gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATg                                                                                            >  2:6714837/1‑93 (MQ=255)

   cATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGa                                                                                           <  1:3499858/93‑1 (MQ=255)

   cATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGa                                                                                           <  2:5689553/93‑1 (MQ=255)

    aTGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGaa                                                                                          <  2:6241985/93‑1 (MQ=255)

     tGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGaaa                                                                                         <  1:4537521/93‑1 (MQ=255)

      ggTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGGTACCTGCATGaaa                                                                                         >  1:2416083/1‑93 (MQ=255)

      ggTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGaaaa                                                                                        <  1:2665823/93‑1 (MQ=255)

           aGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCa                                                                                   <  1:7325286/93‑1 (MQ=255)

            gTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCAt                                                                                  <  2:3837843/93‑1 (MQ=255)

              cGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGt                                                                                <  1:1026504/93‑1 (MQ=255)

               gCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGtt                                                                               <  2:5516790/93‑1 (MQ=255)

                cTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTg                                                                              >  1:6899770/1‑93 (MQ=255)

                 tGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGc                                                                             >  2:6006245/1‑93 (MQ=255)

                 tGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGc                                                                             <  2:3012711/93‑1 (MQ=255)

                    aTGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCgg                                                                          >  1:151599/1‑93 (MQ=255)

                      gTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGaa                                                                        <  2:4437169/93‑1 (MQ=255)

                      gTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGaa                                                                        <  1:1591346/93‑1 (MQ=255)

                            gTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCagag                                                                  >  2:1444412/1‑93 (MQ=255)

                             ttGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGc                                                                 >  2:314082/1‑93 (MQ=255)

                              tGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGca                                                                >  1:3127375/1‑93 (MQ=255)

                              tGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGca                                                                >  2:1897831/1‑93 (MQ=255)

                               gCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaa                                                               <  2:3495376/93‑1 (MQ=255)

                                ccTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaac                                                              <  2:1833352/93‑1 (MQ=255)

                                 cTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaaca                                                             >  1:5082393/1‑93 (MQ=255)

                                 cTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaaca                                                             >  1:2291976/1‑93 (MQ=255)

                                 cTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaaca                                                             >  1:2004757/1‑93 (MQ=255)

                                  tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                            >  1:1172289/1‑93 (MQ=255)

                                  tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                            <  1:4431851/93‑1 (MQ=255)

                                     gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                                         >  1:1107237/1‑93 (MQ=255)

                                     gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GCACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                                         >  2:4888058/1‑93 (MQ=255)

                                     gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GCACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                                         >  2:5657946/1‑93 (MQ=255)

                                      cccTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GCACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGcc                                                        <  2:3609050/93‑1 (MQ=255)

                                       ccTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCt                                                       >  2:584866/1‑93 (MQ=255)

                                        cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                                      >  2:2786066/1‑93 (MQ=255)

                                        cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                                      >  1:3032497/1‑93 (MQ=255)

                                        cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                                      >  1:4207276/1‑93 (MQ=255)

                                        cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                                      >  1:3331693/1‑93 (MQ=255)

                                         tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                                     <  2:2534567/93‑1 (MQ=255)

                                         tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                                     <  1:1317374/93‑1 (MQ=255)

                                          cATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgtt                                                    >  1:3775008/1‑93 (MQ=255)

                                           aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttg                                                   >  1:875534/1‑93 (MQ=255)

                                           aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttg                                                   >  1:6785340/1‑93 (MQ=255)

                                           aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttg                                                   >  1:7136952/1‑93 (MQ=255)

                                            ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                                  >  1:2577306/1‑93 (MQ=255)

                                            ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                                  <  2:2257290/93‑1 (MQ=255)

                                             tCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTgttgtt                                                 >  2:2063094/1‑93 (MQ=255)

                                              cTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGttt                                                <  1:4737770/93‑1 (MQ=255)

                                               tttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTg                                               >  1:7353052/1‑93 (MQ=255)

                                               tttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTg                                               >  2:7245109/1‑93 (MQ=255)

                                               tttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTg                                               >  2:5271096/1‑93 (MQ=255)

                                                 tACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGaa                                             <  1:6413542/93‑1 (MQ=255)

                                                 tACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGaa                                             <  1:4471989/93‑1 (MQ=255)

                                                   cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                           >  2:1016875/1‑93 (MQ=255)

                                                   cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                           >  1:1779664/1‑93 (MQ=255)

                                                    gAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggc                                          >  1:5917927/1‑93 (MQ=255)

                                                    gAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggc                                          >  1:2630090/1‑93 (MQ=255)

                                                     aaGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcg                                         >  1:5650030/1‑93 (MQ=255)

                                                      aGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcgg                                        >  2:4661619/1‑93 (MQ=255)

                                                       gCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcggc                                       <  1:534090/93‑1 (MQ=255)

                                                       gCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcggc                                       >  2:778544/1‑93 (MQ=255)

                                                        cccAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCt                                      >  2:4098870/1‑93 (MQ=255)

                                                        cccAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCt                                      >  1:7243839/1‑93 (MQ=255)

                                                         ccAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTa                                     <  1:7458470/93‑1 (MQ=255)

                                                         ccAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTa                                     >  2:1541969/1‑93 (MQ=255)

                                                          cAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTAt                                    >  2:4535666/1‑93 (MQ=255)

                                                           aGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATc                                   >  1:6011413/1‑93 (MQ=255)

                                                           aGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATc                                   <  1:5477811/93‑1 (MQ=255)

                                                            gCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATcc                                  >  2:3340139/1‑93 (MQ=255)

                                                              gTCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCgg                                >  2:6835962/1‑93 (MQ=255)

                                                               tCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGt                               <  1:684941/93‑1 (MQ=255)

                                                               tCAGCGGCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGt                               <  1:2509290/93‑1 (MQ=255)

                                                                    ggCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTgg                          <  2:117399/93‑1 (MQ=255)

                                                                     gCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGc                         >  2:2379848/1‑93 (MQ=255)

                                                                     gCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGc                         >  1:4801749/1‑93 (MQ=255)

                                                                     gCAAAATGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGc                         >  1:4063359/1‑93 (MQ=255)

                                                                       aaaaTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGa                       >  1:6267639/1‑93 (MQ=255)

                                                                       aaaaTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGa                       >  1:3742229/1‑93 (MQ=255)

                                                                        aaaTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaa                      >  1:906202/1‑93 (MQ=255)

                                                                        aaaTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaa                      >  2:2656098/1‑93 (MQ=255)

                                                                         aaTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaaa                     <  1:6651746/93‑1 (MQ=255)

                                                                          aTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAg                    >  2:7064938/1‑93 (MQ=255)

                                                                          aTGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAg                    <  1:4114383/93‑1 (MQ=255)

                                                                           tGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGt                   <  2:5145014/93‑1 (MQ=255)

                                                                           tGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGt                   <  2:5930079/93‑1 (MQ=255)

                                                                           tGCAGGCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGt                   >  1:2847175/1‑93 (MQ=255)

                                                                              aGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGc                >  1:5281560/1‑93 (MQ=255)

                                                                                gCAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaa              <  1:1208794/93‑1 (MQ=255)

                                                                                 cAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaaa             <  2:4248030/93‑1 (MQ=255)

                                                                                 cAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaaa             >  1:3599960/1‑93 (MQ=255)

                                                                                 cAT‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaaa             >  2:4568627/1‑93 (MQ=255)

                                                                                   t‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAc           >  2:3165751/1‑93 (MQ=255)

                                                                                   t‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAc           <  1:584453/93‑1 (MQ=255)

                                                                                   t‑GTACCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAc           >  1:6726874/1‑93 (MQ=255)

                                                                                       aCCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTCAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCa        <  1:6208097/93‑1 (MQ=255)

                                                                                       aCCTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCa        <  2:1361133/93‑1 (MQ=255)

                                                                                         cTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCaaa      <  1:2088533/93‑1 (MQ=255)

                                                                                         cTGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCaaa      >  2:3265016/1‑93 (MQ=255)

                                                                                          tGCATGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAg     >  2:5100170/1‑93 (MQ=255)

                                                                                             gTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGgcc  >  2:164166/1‑93 (MQ=255)

CGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCAT‑GTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCC  >  NC_000913/3822567‑3822751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`