BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,236	T→C	V85A (GTC→GCC)	yidS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,236	0	T	C	86.1%	819.6 / 117.9	346	V85A (GTC→GCC)	yidS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (21/27); new base C (137/161); total (158/188)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 350 total aligned reads displayed.

GGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAA  >  NC_000913/3867145‑3867327

ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTc                                                                                            >  2:246013/1‑93 (MQ=255)

    tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCaaaa                                                                                        >  2:5273868/1‑93 (MQ=255)

    tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCaaaa                                                                                        >  1:1606597/1‑93 (MQ=255)

    tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCaaaa                                                                                        <  2:6216862/93‑1 (MQ=255)

     gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGGCGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAc                                                                                       <  2:499598/93‑1 (MQ=255)

     gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAc                                                                                       >  2:7263163/1‑93 (MQ=255)

      ctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAcc                                                                                      <  1:5025382/93‑1 (MQ=255)

        ggCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGt                                                                                    <  2:4620654/93‑1 (MQ=255)

        ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTTAGCGCCAAAACCGt                                                                                    <  2:6446407/93‑1 (MQ=255)

        ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGt                                                                                    <  1:3535257/93‑1 (MQ=255)

        ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGt                                                                                    <  2:5703441/93‑1 (MQ=255)

        ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGt                                                                                    <  2:3110769/93‑1 (MQ=255)

         gCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTc                                                                                   >  1:2713275/1‑93 (MQ=255)

           nncGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGa                                                                                 <  2:2295114/91‑1 (MQ=255)

             cGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACg                                                                               >  2:1829194/1‑93 (MQ=255)

              ggACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGt                                                                              <  1:1961778/93‑1 (MQ=255)

                aCGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcg                                                                            <  2:272557/93‑1 (MQ=255)

                 cgcgCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgc                                                                           <  1:3477933/93‑1 (MQ=255)

                  gcgcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgcc                                                                          <  2:3200545/93‑1 (MQ=255)

                   cgcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccg                                                                         <  2:654056/93‑1 (MQ=255)

                    gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcgccgc                                                                        >  1:1090071/1‑93 (MQ=255)

                    gcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccgc                                                                        >  2:288235/1‑93 (MQ=255)

                    gcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccgc                                                                        >  1:5964321/1‑93 (MQ=255)

                      aGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCAt                                                                      <  1:235953/93‑1 (MQ=255)

                       gCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATc                                                                     >  2:3003379/1‑93 (MQ=255)

                         gATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGc                                                                   <  2:3354051/93‑1 (MQ=255)

                           tCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTc                                                                 <  1:3258233/93‑1 (MQ=255)

                            cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa                                                                >  1:4897795/1‑93 (MQ=255)

                            cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa                                                                >  1:2219630/1‑93 (MQ=255)

                            cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa                                                                <  2:112796/93‑1 (MQ=255)

                             ttttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCAc                                                               >  1:6070518/1‑93 (MQ=255)

                             ttttATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAc                                                               >  1:2494448/1‑93 (MQ=255)

                               ttATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAcgc                                                             <  2:5052580/93‑1 (MQ=255)

                                 aTTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt                                                           <  1:6430253/93‑1 (MQ=255)

                                 aTTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt                                                           >  2:345401/1‑93 (MQ=255)

                                  ttCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTa                                                          >  1:611511/1‑93 (MQ=255)

                                  ttCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTa                                                          >  2:1040131/1‑93 (MQ=255)

                                        acgggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTAcc                                                    >  1:2917410/3‑93 (MQ=255)

                                         cgggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCa                                                   <  2:6764217/92‑1 (MQ=255)

                                          gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAg                                                  <  1:181119/93‑1 (MQ=255)

                                          gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAg                                                  <  1:2115677/93‑1 (MQ=255)

                                          gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAg                                                  <  1:6282119/93‑1 (MQ=255)

                                           ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                                                 >  1:5312883/1‑93 (MQ=255)

                                             cTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGa                                               <  1:130202/93‑1 (MQ=255)

                                              tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                              <  1:5457003/93‑1 (MQ=255)

                                              tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                              <  1:4209773/93‑1 (MQ=255)

                                              tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                              >  2:1390957/1‑93 (MQ=255)

                                              tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                              <  1:5686793/93‑1 (MQ=255)

                                               gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                             >  1:6965612/1‑93 (MQ=255)

                                               gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                             >  2:1590908/1‑93 (MQ=255)

                                                aCGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGc                                            <  2:6259369/93‑1 (MQ=255)

                                                 cGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCt                                           >  2:3382789/1‑93 (MQ=255)

                                                  gCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCta                                          <  1:760623/93‑1 (MQ=255)

                                                   cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtat                                         <  1:3734948/93‑1 (MQ=255)

                                                    ttCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtata                                        <  2:5461772/93‑1 (MQ=255)

                                                    ttCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtata                                        <  2:6621978/93‑1 (MQ=255)

                                                    ttCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtata                                        <  1:3138468/93‑1 (MQ=255)

                                                    ttCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtata                                        >  2:5932113/1‑93 (MQ=255)

                                                     tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTatat                                       <  1:5495610/93‑1 (MQ=255)

                                                     tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTatat                                       <  2:6408838/93‑1 (MQ=255)

                                                      ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                      >  1:2651882/1‑93 (MQ=255)

                                                      ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                      <  2:7072318/93‑1 (MQ=255)

                                                      ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                      <  1:2995652/93‑1 (MQ=255)

                                                      ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                      <  1:1473145/93‑1 (MQ=255)

                                                       cTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCa                                     >  1:1907578/1‑93 (MQ=255)

                                                       cTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGTGAAGCTATATCa                                     >  1:6893133/1‑93 (MQ=255)

                                                         gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAat                                   <  2:5320345/93‑1 (MQ=255)

                                                         gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAat                                   >  1:7127952/1‑93 (MQ=255)

                                                          tCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAata                                  <  2:2544475/93‑1 (MQ=255)

                                                            gATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATAtt                                >  1:3885561/1‑93 (MQ=255)

                                                            gATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATAtt                                >  1:3070621/1‑93 (MQ=255)

                                                             aTGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTa                               >  2:2026874/1‑93 (MQ=255)

                                                              tgtgATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTaa                              <  2:5518773/93‑1 (MQ=255)

                                                                   tCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGcc                         >  2:4268347/1‑93 (MQ=255)

                                                                    nncTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCa                        <  2:68408/91‑1 (MQ=255)

                                                                    cGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCa                        <  2:3977238/93‑1 (MQ=255)

                                                                    cGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCa                        <  2:2205161/93‑1 (MQ=255)

                                                                    cGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCa                        <  2:3252439/93‑1 (MQ=255)

                                                                     gCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAt                       >  1:3627012/1‑93 (MQ=255)

                                                                     gCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAt                       >  1:7411593/1‑93 (MQ=255)

                                                                       tAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGc                     >  2:6021528/1‑93 (MQ=255)

                                                                       tAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGc                     >  2:4383098/1‑93 (MQ=255)

                                                                       tAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGc                     >  2:3590132/1‑93 (MQ=255)

                                                                        aaTCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCt                    >  2:7054804/1‑93 (MQ=255)

                                                                          tCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCttt                  <  2:5888191/93‑1 (MQ=255)

                                                                           ccGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTc                 <  1:3311455/93‑1 (MQ=255)

                                                                           ccGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTc                 <  1:4606936/93‑1 (MQ=255)

                                                                             gCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGa               >  1:7026477/1‑93 (MQ=255)

                                                                              cAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc              >  2:2599233/1‑93 (MQ=255)

                                                                              cAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc              >  2:4152378/1‑93 (MQ=255)

                                                                                 aTTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtg           <  1:4375040/93‑1 (MQ=255)

                                                                                  ttttCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt          <  2:3853631/93‑1 (MQ=255)

                                                                                  ttttCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt          >  1:4811920/1‑93 (MQ=255)

                                                                                  ttttCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt          >  1:5125797/1‑93 (MQ=255)

                                                                                  ttttCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt          >  2:2360117/1‑93 (MQ=255)

                                                                                   tttCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgtg         <  1:1783131/93‑1 (MQ=255)

                                                                                     tCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGa       >  2:7433901/1‑93 (MQ=255)

                                                                                      cAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAt      <  1:5242146/93‑1 (MQ=255)

                                                                                        gcgcCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGa    <  2:1313082/93‑1 (MQ=255)

                                                                                          gTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGaaa  <  1:7439235/93‑1 (MQ=255)

GGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAA  >  NC_000913/3867145‑3867327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`