BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,876,595	G→C	P385A (CCG→GCG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,876,595	0	G	C	98.9%	770.4 / NA	266	P385A (CCG→GCG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (3/0); new base C (119/143); total (123/143)
Fisher's exact test for biased strand distribution p-value = 9.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

GATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGGCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGT  >  NC_000913/3876505‑3876687

gATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCCTTTTACGCGCGCGGCgcgccg                                                                                             >  1:4441346/1‑93 (MQ=255)

gATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACgcgccg                                                                                             >  2:3664415/1‑93 (MQ=255)

 aTCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGGTTTTTTCCCGCGCGACGcgccgc                                                                                            >  1:4742332/1‑93 (MQ=255)

  tCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCt                                                                                           <  2:1002158/93‑1 (MQ=255)

      gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGGCGCTTcac                                                                                       >  2:6739025/1‑93 (MQ=255)

      gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcac                                                                                       >  2:5759606/1‑93 (MQ=255)

      gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcac                                                                                       <  1:3623989/93‑1 (MQ=255)

      gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcac                                                                                       <  1:2885154/93‑1 (MQ=255)

      gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTcac                                                                                       >  2:3532799/1‑93 (MQ=255)

       ttCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACCCGCGCGACGCGCCCCCTcacc                                                                                      >  1:971607/1‑92 (MQ=255)

       ttCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCCTctcc                                                                                      >  2:6570015/1‑90 (MQ=255)

        tCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgg                                                                                     <  2:939964/93‑1 (MQ=255)

         ccTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacggg                                                                                    <  2:4932038/93‑1 (MQ=255)

         ccTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacggg                                                                                    <  2:2180220/93‑1 (MQ=255)

           tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggca                                                                                  <  2:539900/93‑1 (MQ=255)

           tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggca                                                                                  <  1:380013/93‑1 (MQ=255)

           tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcaccggca                                                                                  >  2:553146/1‑93 (MQ=255)

            ggCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTGCCCTCGCGACGGGCCGCCTcacgggggg                                                                                 >  1:5914291/1‑90 (MQ=255)

               aGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTACGCGCGCGGCGCGCCGCTTccccgggacggg                                                                              >  1:5879259/1‑93 (MQ=255)

                gTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCacgggcacgggc                                                                             <  2:2923627/93‑1 (MQ=255)

                gTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCaccggcaccggc                                                                             >  2:1420035/1‑93 (MQ=255)

                 tCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCAcgggcacgggca                                                                            <  1:7076611/93‑1 (MQ=255)

                    gCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagca                                                                         <  1:463302/93‑1 (MQ=255)

                        gTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCCTTTCACGCGCGCGACGCGACCCTTCACGGGCACGGGGAGCATccg                                                                     >  2:6183748/1‑91 (MQ=255)

                        gTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGa                                                                     <  1:5436653/93‑1 (MQ=255)

                         tttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑t                                                                   >  2:3986458/1‑93 (MQ=255)

                         tttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGGCGCGCCGCCTCACGGGCCCGGGGAGCATCCA‑t                                                                   >  1:3181014/1‑93 (MQ=255)

                          ttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑Ta                                                                  <  2:7276644/93‑1 (MQ=255)

                          ttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑Ta                                                                  <  2:2008788/93‑1 (MQ=255)

                          ttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑Ta                                                                  <  1:2105782/93‑1 (MQ=255)

                           tGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑Taa                                                                 <  2:710440/93‑1 (MQ=255)

                              ccGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAttt                                                              <  1:458194/93‑1 (MQ=255)

                              ccGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAttt                                                              <  1:6486159/93‑1 (MQ=255)

                              ccGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAttt                                                              <  2:211092/93‑1 (MQ=255)

                               cGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAtttt                                                             <  1:1210402/93‑1 (MQ=255)

                               cGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAtttt                                                             <  1:7002147/93‑1 (MQ=255)

                               cGGCAGGCCCACTAAGTAGAGCGCTCCTTTACGGCGGGTCATTTCACGGGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAtttt                                                             <  1:7292301/93‑1 (MQ=255)

                                 acAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGc                                                           >  2:1538897/2‑93 (MQ=255)

                                   aGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGCCGCGCCCCCTCACCGGCACGGGCAGCACCGA‑TAATTTTTCCa                                                         >  1:6040152/1‑93 (MQ=255)

                                    ggCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCaa                                                        <  1:4859086/93‑1 (MQ=255)

                                    ggCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCACCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCaa                                                        <  1:6219651/93‑1 (MQ=255)

                                     gCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGGATTTTACCCGCGCGGCGCGCCGCCTCACCGGGACGGGGCGGAACGA‑TAAATTTTTCAAc                                                       >  1:1399655/1‑93 (MQ=255)

                                      cccGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAAcc                                                      <  2:5158822/93‑1 (MQ=255)

                                       ccGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCa                                                     >  2:4598669/1‑93 (MQ=255)

                                       ccGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCa                                                     <  1:3425726/93‑1 (MQ=255)

                                       ccGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCa                                                     <  2:4441479/93‑1 (MQ=255)

                                        cGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCAc                                                    <  1:686983/93‑1 (MQ=255)

                                         gCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACg                                                   >  2:2559733/1‑93 (MQ=255)

                                         gCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACCGGGACGGGCAGCATCCA‑TAATTTTTCCAACCAcc                                                   >  1:3390944/1‑92 (MQ=255)

                                          cTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACCGGCAGCATCGA‑TAATTTTGCCAAACACGa                                                  >  2:390554/1‑93 (MQ=255)

                                          cTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTACGCGCGGGACGCGCCGCTTCCCGGGCACGGGCAGCAACGA‑TAAATTTTCCAACCACGa                                                  >  1:7148433/1‑93 (MQ=255)

                                           tAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGGCGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAAATTTTCCAACCACGAt                                                 >  2:5019479/1‑93 (MQ=255)

                                           tAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGCCGCGCCGCCTCACGGGCACGGGCAGCATCGA‑TAATTTTTCCAACCACGAt                                                 >  1:7428006/1‑93 (MQ=255)

                                                 gAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCCCTTCACGGGCACGGGCAGCATCGA‑TAATTTTTTCCACCACGAttttttc                                           >  1:426683/1‑90 (MQ=255)

                                                  aGCGCACCTTTACGGCGGGTCATTTCCCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTcgcg                                          >  2:6671949/1‑93 (MQ=255)

                                                   gcgcACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACCGGCAGCATCGA‑TAATTTTGCCAAACACGATTTTCGCGt                                         >  2:6884323/1‑93 (MQ=255)

                                                     gcACCTTTACGGCGGGGCATTTTACGCGCGCGGCGCGCCGCTTCACGGGCGCGGGCCGCATCGA‑TAATTTTTGCAACCACGATTTTTGCGTCg                                       >  1:5950478/1‑93 (MQ=255)

                                                      cACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCgg                                      <  2:1456618/93‑1 (MQ=255)

                                                      cACCTTTACGGCGGCTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCgg                                      <  2:4376074/93‑1 (MQ=255)

                                                       aCCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGt                                     <  2:4276029/93‑1 (MQ=255)

                                                       aCCTTTACGGCGGGGCATTTCACGCGCGGGACGCGCCCCTTTACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTTGCGTCTGt                                     >  2:3086060/1‑93 (MQ=255)

                                                        ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCgggt                                    >  2:5853273/1‑91 (MQ=255)

                                                         cTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTg                                   <  1:3252802/93‑1 (MQ=255)

                                                          tttACGGCGGGGCATTTTACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTTCCAACCACGATTTTTGCGTCGGTTgg                                  >  1:1101500/1‑93 (MQ=255)

                                                           ttACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTggg                                 <  2:1902188/93‑1 (MQ=255)

                                                            tACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGt                                <  2:1756580/93‑1 (MQ=255)

                                                             aCGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGtt                               <  1:4903694/93‑1 (MQ=255)

                                                             aCGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGtt                               <  2:644060/93‑1 (MQ=255)

                                                                gcggGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTc                            >  1:2699210/1‑93 (MQ=255)

                                                                  gggTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                          <  1:1200848/93‑1 (MQ=255)

                                                                    gTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagc                        >  2:3801381/1‑93 (MQ=255)

                                                                    gTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagc                        >  2:4687580/1‑93 (MQ=255)

                                                                     tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                       <  2:2378969/93‑1 (MQ=255)

                                                                     tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                       <  1:1765509/93‑1 (MQ=255)

                                                                     tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                       >  2:7345970/1‑93 (MQ=255)

                                                                     tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                       >  1:6636691/1‑93 (MQ=255)

                                                                     tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                       >  2:5298573/1‑93 (MQ=255)

                                                                      cATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagcag                      <  2:1110690/93‑1 (MQ=255)

                                                                      cATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagcag                      <  1:406939/93‑1 (MQ=255)

                                                                       aTTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAgg                     <  2:5699137/93‑1 (MQ=255)

                                                                        tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                    >  2:2291249/1‑93 (MQ=255)

                                                                        tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                    <  1:1804400/93‑1 (MQ=255)

                                                                        tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                    >  1:2256749/1‑93 (MQ=255)

                                                                          tCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTAt                  <  2:849735/93‑1 (MQ=255)

                                                                            aCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc                <  2:2706555/93‑1 (MQ=255)

                                                                            aCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc                <  2:5256733/93‑1 (MQ=255)

                                                                            aCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc                <  1:2785851/93‑1 (MQ=255)

                                                                             cgcgcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCt               <  1:1858544/93‑1 (MQ=255)

                                                                             cgcgcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCt               <  1:3029379/93‑1 (MQ=255)

                                                                              gcgcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTg              >  1:5058906/1‑93 (MQ=255)

                                                                                 cgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCca           >  1:2986423/1‑93 (MQ=255)

                                                                                   cgACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagc         <  1:5633816/93‑1 (MQ=255)

                                                                                    gACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca        >  2:3353206/1‑93 (MQ=255)

                                                                                    gACGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca        <  1:87450/93‑1 (MQ=255)

                                                                                     aCGCGCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagcag       >  1:3857242/1‑93 (MQ=255)

                                                                                      cgcgCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgt      <  1:5211073/93‑1 (MQ=255)

                                                                                      cgcgCCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgt      <  1:4153509/93‑1 (MQ=255)

                                                                                       gcgccgCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt     <  2:7170321/93‑1 (MQ=255)

                                                                                       gcgccgCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt     <  1:1018217/93‑1 (MQ=255)

                                                                                        cggcgCCTCAAGGGGCCGGGCAATATCCATTATTTTTGCAAACCCCG‑TTTTTGCGGCGGTTTGGGTTTCCAGCAGGTATTCTGCCAGCAgttc    <  2:7475696/93‑1 (MQ=255)

GATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGGCGCTTCACGGGCACGGGCAGCATCGA‑TAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGT  >  NC_000913/3876505‑3876687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`