BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,435	G→A	A196V (GCG→GTG)	b3718 ←	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,435	0	G	A	86.6%	803.3 / 73.7	292	A196V (GCG→GTG)	b3718	putative isomerase
Reads supporting (aligned to +/- strand): ref base G (19/20); new base A (141/111); total (161/131)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

Only 100 of 297 total aligned reads displayed.

ATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCC  >  NC_000913/3896345‑3896526

 tCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCacgc                                                                                          <  2:851547/93‑1 (MQ=255)

 tCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCacgc                                                                                          >  1:4790251/1‑93 (MQ=255)

   aGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCAcgccg                                                                                        <  1:494050/93‑1 (MQ=255)

     cGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCt                                                                                      <  1:522528/93‑1 (MQ=255)

      gAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTg                                                                                     >  1:5551296/1‑93 (MQ=255)

      gAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTg                                                                                     >  1:3813554/1‑93 (MQ=255)

        aGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGAc                                                                                   >  2:6445978/1‑93 (MQ=255)

         ggATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACa                                                                                  >  1:2050692/1‑93 (MQ=255)

         ggATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACa                                                                                  <  1:3671087/93‑1 (MQ=255)

         ggATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACa                                                                                  >  2:4263280/1‑93 (MQ=255)

          gATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACaa                                                                                 >  2:3227419/1‑93 (MQ=255)

          gATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACaa                                                                                 >  1:5773613/1‑93 (MQ=255)

          gATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACaa                                                                                 <  2:7324640/93‑1 (MQ=255)

          gATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACaa                                                                                 >  2:1408264/1‑93 (MQ=255)

             gCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTa                                                                              <  1:6699680/93‑1 (MQ=255)

               aGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATc                                                                            <  2:3069024/93‑1 (MQ=255)

               aGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATc                                                                            <  2:4713305/93‑1 (MQ=255)

                gCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCa                                                                           <  1:7201193/93‑1 (MQ=255)

                  tGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGc                                                                         >  2:1237412/1‑93 (MQ=255)

                    cAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCaa                                                                       <  1:5347149/93‑1 (MQ=255)

                      acacGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGt                                                                     <  2:5975564/93‑1 (MQ=255)

                      acacGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGt                                                                     >  2:6609838/1‑93 (MQ=255)

                       cacGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGtt                                                                    <  2:4498959/93‑1 (MQ=255)

                         cGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGtttt                                                                  <  1:3134190/93‑1 (MQ=255)

                           gAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTc                                                                <  2:6311224/93‑1 (MQ=255)

                             aGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTcgc                                                              >  2:553595/1‑93 (MQ=255)

                              gCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTcgcc                                                             >  1:2671955/1‑93 (MQ=255)

                                tGGAACATCTTCGGTAACCGGACCTCGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTcgccgc                                                           >  2:6265718/1‑93 (MQ=255)

                                  gAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCa                                                         <  1:5620530/93‑1 (MQ=255)

                                    aCATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatg                                                       >  1:1094764/1‑93 (MQ=255)

                                     cATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatga                                                      >  2:1572878/1‑93 (MQ=255)

                                     cATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatga                                                      <  1:4469529/93‑1 (MQ=255)

                                     cATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatga                                                      <  2:3316576/93‑1 (MQ=255)

                                     cATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatga                                                      >  2:2173084/1‑93 (MQ=255)

                                      aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgat                                                     <  2:3927694/93‑1 (MQ=255)

                                      aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgat                                                     >  2:3660653/1‑93 (MQ=255)

                                       tCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgatg                                                    <  2:4970355/93‑1 (MQ=255)

                                       tCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgatg                                                    >  1:1479735/1‑93 (MQ=255)

                                       tCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgatg                                                    >  1:1826553/1‑93 (MQ=255)

                                       tCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgatg                                                    >  2:2232743/1‑93 (MQ=255)

                                        cTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGc                                                   <  1:7067351/93‑1 (MQ=255)

                                         ttCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCt                                                  >  1:636694/1‑93 (MQ=255)

                                          tCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCtt                                                 >  1:6733909/1‑93 (MQ=255)

                                            ggTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCtttt                                               <  1:5931472/93‑1 (MQ=255)

                                            ggTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCtttt                                               <  1:7270629/93‑1 (MQ=255)

                                             gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTCCCCGCGCCGCTGACAATTATCAGCAAGTGTTTCGCCGCCATGATGCTTTTc                                              <  1:3462614/93‑1 (MQ=255)

                                             gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTc                                              >  1:4186870/1‑93 (MQ=255)

                                             gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTc                                              >  1:1318048/1‑93 (MQ=255)

                                             gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTc                                              <  2:7022144/93‑1 (MQ=255)

                                              tAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCg                                             >  2:2447774/1‑93 (MQ=255)

                                               aaCCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCgg                                            <  2:5821655/93‑1 (MQ=255)

                                                aCCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGc                                           >  2:2924252/1‑93 (MQ=255)

                                                aCCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGc                                           >  1:82823/1‑93 (MQ=255)

                                                aCCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGc                                           >  1:2782445/1‑93 (MQ=255)

                                                 ccGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcc                                          <  2:3435079/93‑1 (MQ=255)

                                                 ccGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcc                                          >  2:5801849/1‑93 (MQ=255)

                                                  cGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGccc                                         <  1:1027806/93‑1 (MQ=255)

                                                  cGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGccc                                         >  2:1177391/1‑93 (MQ=255)

                                                   ggACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcccc                                        <  1:1810623/93‑1 (MQ=255)

                                                   ggACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcccc                                        >  2:4072863/1‑93 (MQ=255)

                                                    gACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCa                                       >  2:1116019/1‑93 (MQ=255)

                                                    gACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCa                                       >  1:2120782/1‑93 (MQ=255)

                                                     aCCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCAt                                      <  2:2761944/93‑1 (MQ=255)

                                                      ccTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATc                                     <  2:5478715/93‑1 (MQ=255)

                                                       cTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCg                                    >  1:2342429/1‑93 (MQ=255)

                                                        ttGCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGt                                   <  2:2522971/93‑1 (MQ=255)

                                                          gCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCa                                 <  1:7115706/93‑1 (MQ=255)

                                                          gCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCa                                 <  2:5665651/93‑1 (MQ=255)

                                                          gCAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCa                                 <  2:6839437/93‑1 (MQ=255)

                                                           cAGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCAc                                >  1:1243434/1‑93 (MQ=255)

                                                            aGAACATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACg                               >  2:187991/1‑93 (MQ=255)

                                                            aGAACATTTTTCAGCGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACg                               >  1:6148063/1‑93 (MQ=255)

                                                              aaCATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTa                             <  1:2881311/93‑1 (MQ=255)

                                                               aCATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAg                            >  2:3821151/1‑93 (MQ=255)

                                                                cATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGc                           >  2:7108235/1‑93 (MQ=255)

                                                                cATTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGc                           >  1:3088050/1‑93 (MQ=255)

                                                                 aTTTTTCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCt                          <  2:3500052/93‑1 (MQ=255)

                                                                  tttttCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTg                         >  1:6920993/1‑93 (MQ=255)

                                                                  tttttCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAATTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTg                         >  1:5124204/1‑93 (MQ=255)

                                                                     ttCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCa                      <  2:1622910/93‑1 (MQ=255)

                                                                     ttCAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTGTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCa                      <  1:5681343/93‑1 (MQ=255)

                                                                       cAGTGCCTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAgg                    >  1:3479502/1‑93 (MQ=255)

                                                                         gTGACTGCGCTTTACCCACGCCGCTGACAATTATAAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTAACGTAGCTGTCAGGCa                  >  1:4644446/1‑93 (MQ=255)

                                                                          tGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCAc                 <  1:6528535/93‑1 (MQ=255)

                                                                            ccTGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTa               >  2:4860967/1‑93 (MQ=255)

                                                                              tGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAAc             <  2:53406/93‑1 (MQ=255)

                                                                              tGCGCTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAAc             >  2:4443418/1‑93 (MQ=255)

                                                                               gcgcTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACg            >  1:3552986/1‑93 (MQ=255)

                                                                               gcgcTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACg            >  1:1517031/1‑93 (MQ=255)

                                                                                 gcTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaa          >  2:1334520/1‑93 (MQ=255)

                                                                                 gcTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaa          >  1:4369575/1‑93 (MQ=255)

                                                                                 gcTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaa          <  2:2157528/93‑1 (MQ=255)

                                                                                  cTTTACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaaa         >  2:1705860/1‑93 (MQ=255)

                                                                                   tttACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaaaa        >  1:2535035/1‑93 (MQ=255)

                                                                                   tttACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaaaa        >  1:6658260/1‑93 (MQ=255)

                                                                                    ttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAg       >  2:5060990/1‑93 (MQ=255)

                                                                                    ttACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAg       <  1:4991276/93‑1 (MQ=255)

                                                                                    ttACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAg       >  2:2367367/1‑93 (MQ=255)

                                                                                     tACCCACGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGt      <  1:400831/93‑1 (MQ=255)

                                                                                         cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTcgcc  >  2:1515543/1‑93 (MQ=255)

ATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCC  >  NC_000913/3896345‑3896526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`