BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,701	T→C	H128R (CAC→CGC)	mioC ←	MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,701	0	T	C	84.6%	733.4 / 127.8	324	H128R (CAC→CGC)	mioC	MioC
Reads supporting (aligned to +/- strand): ref base T (25/25); new base C (134/140); total (159/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 326 total aligned reads displayed.

TTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAG  >  NC_000913/3923609‑3923793

ttATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGt                                                                                              <  2:6111932/93‑1 (MQ=255)

   tCCGCAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGAt                                                                                           >  1:880845/1‑93 (MQ=255)

   tCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGAt                                                                                           <  2:4821928/93‑1 (MQ=255)

    ccACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATc                                                                                          >  2:1603045/1‑93 (MQ=255)

    ccACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATc                                                                                          >  1:7186155/1‑93 (MQ=255)

     cacaGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCa                                                                                         <  2:1775373/93‑1 (MQ=255)

       caGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAg                                                                                       >  2:6565711/1‑93 (MQ=255)

            gATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCGTTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGt                                                                                  <  2:3048796/93‑1 (MQ=255)

            gATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGt                                                                                  <  1:2362915/93‑1 (MQ=255)

             aTCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGtt                                                                                 <  1:7093533/93‑1 (MQ=255)

               cGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGa                                                                               >  2:4636386/1‑93 (MQ=255)

                gCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGAt                                                                              >  1:7433694/1‑93 (MQ=255)

                 cACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATc                                                                             <  1:1003664/93‑1 (MQ=255)

                  aCGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCt                                                                            >  1:2192921/1‑93 (MQ=255)

                   ngATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCtt                                                                           <  2:2705756/92‑1 (MQ=255)

                   cGATCTGTATACTTATTTGAGTAAATTACCCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCtt                                                                           <  1:2867051/93‑1 (MQ=255)

                   cGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCtt                                                                           <  1:5189387/93‑1 (MQ=255)

                   cGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCtt                                                                           >  2:1855425/1‑93 (MQ=255)

                    gATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTc                                                                          >  1:5761307/1‑93 (MQ=255)

                    gATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTc                                                                          <  1:537460/93‑1 (MQ=255)

                    gATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTc                                                                          <  2:867643/93‑1 (MQ=255)

                      tCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAg                                                                        <  2:4299479/93‑1 (MQ=255)

                       cTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgt                                                                       >  1:6659671/1‑93 (MQ=255)

                       cTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgt                                                                       <  2:3264929/93‑1 (MQ=255)

                       cTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgt                                                                       <  1:4237553/93‑1 (MQ=255)

                        tGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtg                                                                      >  2:4189331/1‑93 (MQ=255)

                        tGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtg                                                                      <  2:7105988/93‑1 (MQ=255)

                        tGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtg                                                                      >  2:7383307/1‑93 (MQ=255)

                         gTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtgt                                                                     >  2:1463108/1‑93 (MQ=255)

                         gTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtgt                                                                     <  1:2625925/93‑1 (MQ=255)

                         gTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtgt                                                                     >  2:6969263/1‑93 (MQ=255)

                         gTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtgt                                                                     >  1:1660723/1‑93 (MQ=255)

                         gTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAgtgt                                                                     >  1:5595866/1‑93 (MQ=255)

                          tataCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGtt                                                                    <  2:7029943/93‑1 (MQ=255)

                          tataCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGtt                                                                    >  1:6302932/1‑93 (MQ=255)

                           ataCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGttt                                                                   >  2:397931/1‑93 (MQ=255)

                           ataCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGttt                                                                   <  2:6379447/93‑1 (MQ=255)

                            taCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTc                                                                  >  2:7489826/1‑93 (MQ=255)

                            taCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTc                                                                  >  1:1133647/1‑93 (MQ=255)

                            taCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTc                                                                  <  1:1199043/93‑1 (MQ=255)

                             aCTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCg                                                                 <  2:3902851/93‑1 (MQ=255)

                              cTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGc                                                                >  1:125073/1‑93 (MQ=255)

                               ttATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGcc                                                               <  1:626598/93‑1 (MQ=255)

                               ttATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGcc                                                               >  2:5515297/1‑93 (MQ=255)

                                tATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCct                                                              <  1:1967422/93‑1 (MQ=255)

                                   ttGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCctgtc                                                           <  1:3958671/93‑1 (MQ=255)

                                   ttGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCctgtc                                                           <  1:4744584/93‑1 (MQ=255)

                                   ttGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCctgtc                                                           >  2:2803041/1‑93 (MQ=255)

                                      aGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCctgtctgt                                                        <  1:2471484/93‑1 (MQ=255)

                                       gTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGtt                                                       >  2:5702245/1‑93 (MQ=255)

                                       gTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGtt                                                       <  1:3083713/93‑1 (MQ=255)

                                        tAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGttt                                                      <  2:3501689/93‑1 (MQ=255)

                                        tAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGttt                                                      <  1:2255831/93‑1 (MQ=255)

                                         aaaTTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGtttt                                                     <  2:4657082/93‑1 (MQ=255)

                                          aaTTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTg                                                    >  1:5490955/1‑93 (MQ=255)

                                          aaTTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTg                                                    <  1:5262128/93‑1 (MQ=255)

                                           aTTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGc                                                   <  1:3801074/93‑1 (MQ=255)

                                             tAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCAc                                                 <  2:2257052/93‑1 (MQ=255)

                                              aaCCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCAcc                                                >  1:3696458/1‑93 (MQ=255)

                                                cccACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCgg                                              >  1:6878072/1‑93 (MQ=255)

                                                 ccACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGa                                             >  2:5846384/1‑93 (MQ=255)

                                                   aCGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAt                                           <  2:6005893/93‑1 (MQ=255)

                                                   aCGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAt                                           <  1:4923567/93‑1 (MQ=255)

                                                     gATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAttt                                         >  1:3501686/1‑93 (MQ=255)

                                                       tCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAttttt                                       >  2:5344626/1‑93 (MQ=255)

                                                       tCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAttttt                                       >  2:1237120/1‑93 (MQ=255)

                                                       tCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAAttttt                                       >  1:695982/1‑93 (MQ=255)

                                                         ccAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGa                                     <  2:2411659/93‑1 (MQ=255)

                                                           aGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGt                                   <  2:6755351/93‑1 (MQ=255)

                                                            gCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGtt                                  >  1:3161912/1‑93 (MQ=255)

                                                            gCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGtt                                  >  1:1868242/1‑93 (MQ=255)

                                                            gCCATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGtt                                  >  2:3686208/1‑93 (MQ=255)

                                                             ccATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTc                                 >  1:2732591/1‑93 (MQ=255)

                                                             ccATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTc                                 <  1:1708868/93‑1 (MQ=255)

                                                              cATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTTCACCGGGATTTTTGAGTTCt                                >  1:6175325/1‑93 (MQ=255)

                                                              cATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCt                                >  2:3340780/1‑93 (MQ=255)

                                                              cATTCTTCTGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCt                                <  1:4147135/93‑1 (MQ=255)

                                                               aTTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTg                               >  2:2690025/1‑93 (MQ=255)

                                                                ttcttcTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGc                              >  1:943880/1‑93 (MQ=255)

                                                                 tcttctGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGcc                             <  1:7311278/93‑1 (MQ=255)

                                                                    tctGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCg                          >  2:1018745/1‑93 (MQ=255)

                                                                      tGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAg                        >  1:979339/1‑93 (MQ=255)

                                                                      tGCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAg                        <  1:4470923/93‑1 (MQ=255)

                                                                       gCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGt                       <  1:6548096/93‑1 (MQ=255)

                                                                       gCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGt                       >  1:1337570/1‑93 (MQ=255)

                                                                       gCCGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGt                       <  2:4148411/93‑1 (MQ=255)

                                                                         cGGATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGttt                     >  1:5979879/1‑93 (MQ=255)

                                                                           gATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTAt                   >  2:6303739/1‑93 (MQ=255)

                                                                           gATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTAt                   >  2:1145287/1‑93 (MQ=255)

                                                                           gATCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTAt                   <  1:1494955/93‑1 (MQ=255)

                                                                            aTCTTCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATc                  <  1:4289767/93‑1 (MQ=255)

                                                                               ttCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGAt               <  2:4604475/93‑1 (MQ=255)

                                                                               ttCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGAt               <  2:3211070/93‑1 (MQ=255)

                                                                               ttCCGGAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGAt               >  2:7253125/1‑93 (MQ=255)

                                                                                   ggAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGcc           >  2:2584555/1‑93 (MQ=255)

                                                                                    gAATGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGccc          >  2:2864942/1‑93 (MQ=255)

                                                                                     aaTGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGcccc         <  1:4633769/93‑1 (MQ=255)

                                                                                     aaTGTCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGcccc         >  2:4940142/1‑93 (MQ=255)

                                                                                         tCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACaa     >  2:5585978/1‑93 (MQ=255)

                                                                                         tCGCGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACaa     >  1:3383453/1‑93 (MQ=255)

TTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCAAGAATGTTGATCTTCAGTGTTTCGCCTGTCTGTTTTGCACCGGAATTTTTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAG  >  NC_000913/3923609‑3923793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`