BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,107	A→G	L246P (CTG→CCG)	acrA ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,107	0	A	G	87.3%	691.4 / 85.1	276	L246P (CTG→CCG)	acrA	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (17/18); new base G (117/124); total (134/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

Only 100 of 276 total aligned reads displayed.

AGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTC  >  NC_000913/484017‑484196

aGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGt                                                                                         <  1:908806/93‑1 (MQ=255)

aGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGt                                                                                         >  1:2863351/1‑93 (MQ=255)

 gggTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTg                                                                                        >  2:2038197/1‑93 (MQ=255)

  ggTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATT‑CAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGac                                                                                      <  1:3687804/93‑1 (MQ=255)

     gATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGacac                                                                                    >  2:4489425/1‑93 (MQ=255)

      aTAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACt                                                                                   >  2:7057478/1‑93 (MQ=255)

        aGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACttt                                                                                 <  1:5179453/93‑1 (MQ=255)

        aGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACttt                                                                                 >  1:2177273/1‑93 (MQ=255)

        aGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACttt                                                                                 >  2:2458574/1‑93 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAGCTTAATGCCGTCACTGGTGATCAGTGACACTTTg                                                                                >  2:4180616/1‑93 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTg                                                                                >  2:3336022/1‑93 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTg                                                                                >  2:650286/1‑93 (MQ=255)

            ccAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCt                                                                             >  2:4601658/1‑93 (MQ=255)

             cAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            <  2:4438147/93‑1 (MQ=255)

               gTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACAGTTTGGCTTTg                                                                          >  2:3158939/1‑93 (MQ=255)

                 ggTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGcc                                                                        <  2:5499420/93‑1 (MQ=255)

                  gTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCg                                                                       >  1:7428322/1‑93 (MQ=255)

                     tGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtc                                                                    >  1:6193625/1‑93 (MQ=255)

                       aTCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtctc                                                                  <  1:5897354/93‑1 (MQ=255)

                        tCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTctct                                                                 <  1:1687470/93‑1 (MQ=255)

                          caCGGGAACGACAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTg                                                               <  2:6325490/92‑1 (MQ=255)

                           aCGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGt                                                              <  2:836897/93‑1 (MQ=255)

                           aCGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGt                                                              <  1:6604322/93‑1 (MQ=255)

                            cGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGtt                                                             <  1:3718049/93‑1 (MQ=255)

                             ggTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGttt                                                            >  2:182356/1‑93 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           <  2:2897354/93‑1 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           <  2:2255686/93‑1 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           >  2:1996810/1‑93 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           >  2:5860501/1‑93 (MQ=255)

                               tAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCa                                                          <  2:3542862/93‑1 (MQ=255)

                               tAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCa                                                          <  1:6562501/93‑1 (MQ=255)

                               tAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCa                                                          >  1:1834029/1‑93 (MQ=255)

                                aaCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAg                                                         <  2:4822283/93‑1 (MQ=255)

                                 aCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGc                                                        <  1:6783504/93‑1 (MQ=255)

                                  cGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCg                                                       <  1:594784/93‑1 (MQ=255)

                                  cGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCg                                                       <  1:6426038/93‑1 (MQ=255)

                                   gTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGt                                                      <  1:2113479/93‑1 (MQ=255)

                                    tCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTg                                                     >  2:2597455/1‑93 (MQ=255)

                                      agagAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   <  2:4211341/93‑1 (MQ=255)

                                      agagAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   >  1:3761942/1‑93 (MQ=255)

                                      agagAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   <  2:7532088/93‑1 (MQ=255)

                                      agagAATTCCAGCGTACCGGCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   >  1:624431/1‑93 (MQ=255)

                                        agaATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCAt                                                 <  1:108954/93‑1 (MQ=255)

                                         gaATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCAtt                                                <  1:1916113/93‑1 (MQ=255)

                                         gaATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCAtt                                                <  1:3065119/93‑1 (MQ=255)

                                          aaTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTc                                               >  1:4138061/1‑93 (MQ=255)

                                           aTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCg                                              <  2:254783/93‑1 (MQ=255)

                                            ttCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGc                                             >  1:254197/1‑93 (MQ=255)

                                                aGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGt                                         >  1:786090/1‑93 (MQ=255)

                                                  cGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTc                                       >  1:2583486/1‑93 (MQ=255)

                                                   gTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTcc                                      >  1:4748866/1‑93 (MQ=255)

                                                   gTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTcc                                      <  2:601138/93‑1 (MQ=255)

                                                    tACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCt                                     >  1:3308441/1‑93 (MQ=255)

                                                    tACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCt                                     >  1:2294254/1‑93 (MQ=255)

                                                    tACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCt                                     >  2:5225706/1‑93 (MQ=255)

                                                    tACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCt                                     <  1:7262141/93‑1 (MQ=255)

                                                     aCCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTg                                    >  1:7141225/1‑93 (MQ=255)

                                                     aCCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTg                                    >  1:4620307/1‑93 (MQ=255)

                                                      ccGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGt                                   >  2:6119236/1‑93 (MQ=255)

                                                      ccGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGt                                   <  1:6096666/93‑1 (MQ=255)

                                                       cGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGtt                                  <  2:2095631/93‑1 (MQ=255)

                                                        gTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGttt                                 >  1:5089433/1‑93 (MQ=255)

                                                         tCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACTCTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTc                                <  2:6764117/93‑1 (MQ=255)

                                                         tCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCTCCAGTTCCTGTTTc                                <  2:5188646/93‑1 (MQ=255)

                                                         tCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTc                                >  1:5554517/1‑93 (MQ=255)

                                                         tCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTc                                >  2:1531825/1‑93 (MQ=255)

                                                          ccTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCa                               <  2:4991184/93‑1 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              >  1:4306365/1‑93 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              >  2:3906989/1‑93 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              >  1:1622909/1‑93 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              <  1:6984976/93‑1 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              <  1:1170862/93‑1 (MQ=255)

                                                            tGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAgg                             <  1:1003597/93‑1 (MQ=255)

                                                               gggAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGgcgc                          >  2:3071387/1‑93 (MQ=255)

                                                                ggAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCa                         >  2:3644036/1‑93 (MQ=255)

                                                                 gAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAg                        >  2:5270339/1‑93 (MQ=255)

                                                                  aaCTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAgg                       <  2:1328700/93‑1 (MQ=255)

                                                                   aCTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGACGCAGGa                      <  1:3236117/93‑1 (MQ=255)

                                                                   aCTTAATACCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGa                      >  2:5747052/1‑93 (MQ=255)

                                                                    cTTAATGCCGTCACTGGTGATCGGTGACTCTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGaa                     <  2:6188634/93‑1 (MQ=255)

                                                                    cTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGaa                     <  2:7464406/93‑1 (MQ=255)

                                                                     ttAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAg                    <  1:5726679/93‑1 (MQ=255)

                                                                     ttAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAg                    >  1:5684292/1‑93 (MQ=255)

                                                                      tAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGt                   <  2:1110469/93‑1 (MQ=255)

                                                                        aTGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCg                 >  2:3676397/1‑93 (MQ=255)

                                                                         tGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGt                >  2:6619136/1‑93 (MQ=255)

                                                                         tGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGt                <  1:530474/93‑1 (MQ=255)

                                                                           ccGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTg              >  1:923151/1‑93 (MQ=255)

                                                                            cGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGc             <  1:3154704/93‑1 (MQ=255)

                                                                            cGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGc             >  2:2736863/1‑93 (MQ=255)

                                                                            cGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGc             <  1:542082/93‑1 (MQ=255)

                                                                             gTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCt            >  1:3590406/1‑93 (MQ=255)

                                                                              tCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTg           >  2:3545779/1‑93 (MQ=255)

                                                                                aCTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGa         >  2:1302509/1‑93 (MQ=255)

                                                                                 cTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGAc        >  1:6485444/1‑93 (MQ=255)

                                                                                  tGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACt       >  2:3498195/1‑93 (MQ=255)

                                                                                   ggTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTg      <  1:4350069/93‑1 (MQ=255)

                                                                                     tGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTggg    <  2:5631594/93‑1 (MQ=255)

                                                                                      gATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGt   <  1:5234039/93‑1 (MQ=255)

                                                                                      gATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGt   >  1:1974309/1‑93 (MQ=255)

AGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTC  >  NC_000913/484017‑484196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`