BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	517,700	A→G	V225A (GTG→GCG)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	517,700	0	A	G	82.5%	498.5 / 99.9	242	V225A (GTG→GCG)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (25/17); new base G (96/102); total (121/121)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 266 total aligned reads displayed.

CTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGA  >  NC_000913/517609‑517792

 ttAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCaccgcc                                                                                            >  1:6393439/1‑93 (MQ=255)

 ttAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCaccgcc                                                                                            >  2:1355562/1‑93 (MQ=255)

  tAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCg                                                                                           >  1:3145598/1‑93 (MQ=255)

      gaCCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTc                                                                                       <  1:3754753/92‑1 (MQ=255)

      cACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTc                                                                                       <  1:4212989/93‑1 (MQ=255)

       aCCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTcc                                                                                      <  2:457075/93‑1 (MQ=255)

       aCCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTcc                                                                                      >  1:328473/1‑93 (MQ=255)

        ccATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCg                                                                                     <  1:7349791/93‑1 (MQ=255)

         cATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGTATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCgg                                                                                    >  1:524983/1‑93 (MQ=255)

         cATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCgg                                                                                    >  1:1264746/1‑93 (MQ=255)

           ttACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTc                                                                                  >  1:1349179/1‑93 (MQ=255)

            tACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTcc                                                                                 <  1:7179107/93‑1 (MQ=255)

             aCCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTccc                                                                                >  1:2127949/1‑93 (MQ=255)

             aCCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTccc                                                                                <  1:799186/93‑1 (MQ=255)

              ccGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCa                                                                               <  1:2894464/93‑1 (MQ=255)

              ccGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCa                                                                               <  2:3910543/93‑1 (MQ=255)

               cGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCaa                                                                              <  2:3076682/93‑1 (MQ=255)

                   cAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTa                                                                          >  1:3261230/1‑93 (MQ=255)

                   cAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTa                                                                          >  1:6644346/1‑93 (MQ=255)

                   cAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTa                                                                          >  2:2360963/1‑93 (MQ=255)

                   cAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTa                                                                          >  1:2013250/1‑93 (MQ=255)

                   cAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTa                                                                          >  2:3963488/1‑93 (MQ=255)

                     ggTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTaaa                                                                        <  2:5710834/93‑1 (MQ=255)

                       tCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgc                                                                      >  2:2999913/1‑93 (MQ=255)

                         aCCAGCGTCACTGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgcgg                                                                    >  2:4990475/1‑93 (MQ=255)

                         aCCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgcgg                                                                    >  2:753834/1‑93 (MQ=255)

                          ccAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgcggg                                                                   <  2:3250362/93‑1 (MQ=255)

                           cAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCCCCCCCGCTTCCGGTCCCAACGTAAAgggggg                                                                  <  1:2372243/93‑2 (MQ=255)

                           cAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgcgggc                                                                  <  1:6796518/93‑1 (MQ=255)

                            aGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgggcg                                                                 <  2:3494866/93‑1 (MQ=255)

                             gCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAgcgggcgg                                                                >  1:1003190/1‑93 (MQ=255)

                              cGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc                                                               >  1:2083973/1‑93 (MQ=255)

                                tCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGa                                                             <  2:5904267/93‑1 (MQ=255)

                                tCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGa                                                             >  1:3218953/1‑93 (MQ=255)

                                 cACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGAt                                                            <  2:4407315/93‑1 (MQ=255)

                                 cACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGAt                                                            >  2:2508842/1‑93 (MQ=255)

                                 cACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGAt                                                            <  2:1567700/93‑1 (MQ=255)

                                  aCCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATg                                                           >  2:5075534/1‑93 (MQ=255)

                                  aCCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATg                                                           >  1:3032629/1‑93 (MQ=255)

                                    cGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGcc                                                         <  2:4937891/93‑1 (MQ=255)

                                     ggATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCg                                                        <  1:4239429/93‑1 (MQ=255)

                                     ggATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCg                                                        >  2:1408075/1‑93 (MQ=255)

                                      gATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCCCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCgg                                                       <  2:1217925/93‑1 (MQ=255)

                                       aTAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCggg                                                      >  2:5232351/1‑93 (MQ=255)

                                        tAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGa                                                     >  1:2962173/1‑93 (MQ=255)

                                         aGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCCCCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAt                                                    <  1:1701845/93‑1 (MQ=255)

                                            gcATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAtttt                                                 >  2:7186111/1‑93 (MQ=255)

                                             cATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTc                                                <  2:3892636/93‑1 (MQ=255)

                                              aTCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCg                                               <  2:6547695/93‑1 (MQ=255)

                                               tCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGa                                              >  1:4994458/1‑93 (MQ=255)

                                               tCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGa                                              <  1:931076/93‑1 (MQ=255)

                                               tCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGa                                              >  2:2527944/1‑93 (MQ=255)

                                                cTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCGACCGCCCCTTCCGGCCCCAACGTAAAGCGGGCGGCGCTGCCGGGATTTTCgtc                                             >  1:6210736/1‑91 (MQ=255)

                                                 ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACt                                            >  1:6464748/1‑93 (MQ=255)

                                                 ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACt                                            <  1:7264106/93‑1 (MQ=255)

                                                 ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACt                                            <  1:1541350/93‑1 (MQ=255)

                                                  tCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg                                           <  2:1810098/93‑1 (MQ=255)

                                                  tCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg                                           <  2:1961243/93‑1 (MQ=255)

                                                  tCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg                                           >  2:3168800/1‑93 (MQ=255)

                                                  tCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg                                           <  2:1461558/93‑1 (MQ=255)

                                                    ggCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGt                                         <  1:2726347/93‑1 (MQ=255)

                                                     gCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGtt                                        <  2:4645181/93‑1 (MQ=255)

                                                     gCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGtt                                        <  2:4714899/93‑1 (MQ=255)

                                                     gCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGtt                                        <  2:5620193/93‑1 (MQ=255)

                                                     gCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGtt                                        <  1:1095996/93‑1 (MQ=255)

                                                      cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt                                       <  1:3958264/93‑1 (MQ=255)

                                                       ttCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa                                      >  1:354213/1‑93 (MQ=255)

                                                       ttCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa                                      >  2:6181881/1‑93 (MQ=255)

                                                         ctcGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATc                                    <  1:5143529/93‑1 (MQ=255)

                                                         ctcGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATc                                    >  2:5276790/1‑93 (MQ=255)

                                                          tcGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCa                                   >  1:4351733/1‑93 (MQ=255)

                                                          tcGCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCa                                   >  2:1019451/1‑93 (MQ=255)

                                                            gCTAATAAAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACt                                 >  1:5424586/1‑93 (MQ=255)

                                                              taataaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACttt                               <  2:4285313/93‑1 (MQ=255)

                                                              taataaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACttt                               <  1:4940581/93‑1 (MQ=255)

                                                              taataaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACttt                               <  1:863195/93‑1 (MQ=255)

                                                               aataaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTg                              <  2:552406/93‑1 (MQ=255)

                                                                ataaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGc                             >  2:6455335/1‑93 (MQ=255)

                                                                 taaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCg                            >  2:4187378/1‑93 (MQ=255)

                                                                 taaAAGCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCg                            >  1:3393163/1‑93 (MQ=255)

                                                                    aaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCt                         >  1:7016291/1‑93 (MQ=255)

                                                                      gCATGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTgg                       >  2:3550391/1‑93 (MQ=255)

                                                                        aTGGCGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGtt                     <  1:6524099/93‑1 (MQ=255)

                                                                        aTGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGtt                     >  2:6014722/1‑93 (MQ=255)

                                                                            cGTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACg                 >  2:4105881/1‑93 (MQ=255)

                                                                            cGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACg                 >  2:6261773/1‑93 (MQ=255)

                                                                             gTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGt                <  1:4813724/93‑1 (MQ=255)

                                                                             gTACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGt                <  1:3644292/93‑1 (MQ=255)

                                                                             gTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGt                >  1:5929084/1‑93 (MQ=255)

                                                                              tACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGtt               >  2:7012487/1‑93 (MQ=255)

                                                                              tACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGtt               <  1:3473874/93‑1 (MQ=255)

                                                                              tACTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGtt               >  1:1470046/1‑93 (MQ=255)

                                                                               aCTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTg              >  1:5632040/1‑93 (MQ=255)

                                                                                cTTTGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGt             <  1:584200/93‑1 (MQ=255)

                                                                                 tttGTCCACCGCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTc            >  2:170214/1‑93 (MQ=255)

                                                                                      ccaccgCCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGa       <  2:7273432/93‑1 (MQ=255)

                                                                                       caccgcCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGaa      >  1:2525887/1‑93 (MQ=255)

                                                                                       caccgcCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGaa      >  1:4545241/1‑93 (MQ=255)

                                                                                       caccgcCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGaa      >  2:1365485/1‑93 (MQ=255)

                                                                                        accgccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAg     >  1:5655927/1‑93 (MQ=255)

CTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGA  >  NC_000913/517609‑517792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`