BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,155,035	A→G	T203A (ACG→GCG)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,155,035	0	A	G	84.7%	579.0 / 93.7	262	T203A (ACG→GCG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand): ref base A (14/26); new base G (106/116); total (120/142)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 264 total aligned reads displayed.

ATGTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGT  >  NC_000913/4154943‑4155124

aTGTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGaa                                                                                           <  1:4826641/93‑1 (MQ=255)

 tGTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAAc                                                                                          <  1:6582607/93‑1 (MQ=255)

  gTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCg                                                                                         <  2:4431639/93‑1 (MQ=255)

  gTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCg                                                                                         >  2:7432408/1‑93 (MQ=255)

   ttGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCgg                                                                                        <  1:1430269/93‑1 (MQ=255)

     gagaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGcc                                                                                      <  2:7305164/93‑1 (MQ=255)

      agaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCt                                                                                     <  2:5787097/93‑1 (MQ=255)

        aTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTAt                                                                                   <  2:6567859/93‑1 (MQ=255)

         tGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATg                                                                                  <  1:6817295/93‑1 (MQ=255)

         tGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATg                                                                                  >  2:2495873/1‑93 (MQ=255)

          gCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGa                                                                                 <  1:4898647/93‑1 (MQ=255)

           cTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGaa                                                                                <  1:6022140/93‑1 (MQ=255)

             ggCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAAt                                                                              <  1:5083594/93‑1 (MQ=255)

             ggCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAAt                                                                              >  1:2512592/1‑93 (MQ=255)

               cgcgTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCg                                                                            <  1:4289628/93‑1 (MQ=255)

                  gTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGAcc                                                                         >  1:3860186/1‑93 (MQ=255)

                   tgatgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCg                                                                        >  2:5215465/1‑93 (MQ=255)

                    gatgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGt                                                                       >  1:1758678/1‑93 (MQ=255)

                     atgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTTGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTg                                                                      <  1:3033654/93‑1 (MQ=255)

                     atgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTg                                                                      >  2:1515098/1‑93 (MQ=255)

                      tgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGa                                                                     >  1:1824031/1‑93 (MQ=255)

                       gaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGaa                                                                    >  1:1535066/1‑93 (MQ=255)

                         aaGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACa                                                                  <  1:3835407/93‑1 (MQ=255)

                         aaGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACa                                                                  <  1:4741977/93‑1 (MQ=255)

                          aGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAg                                                                 >  1:2657232/1‑93 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  1:3092915/93‑1 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  1:5730875/93‑1 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  2:3812233/93‑1 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  2:4754909/93‑1 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  1:1692128/93‑1 (MQ=255)

                           gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                <  1:629580/93‑1 (MQ=255)

                            ccGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGtt                                                               <  1:7337709/93‑1 (MQ=255)

                              gTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAg                                                             >  2:896219/1‑93 (MQ=255)

                              gTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAg                                                             >  2:4965830/1‑93 (MQ=255)

                                ttGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCa                                                           <  1:393644/93‑1 (MQ=255)

                                ttGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCa                                                           <  1:3219799/93‑1 (MQ=255)

                                 tGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAg                                                          >  2:6349152/1‑93 (MQ=255)

                                    aGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggct                                                       <  2:3013751/93‑1 (MQ=255)

                                    aGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggct                                                       <  2:6480553/93‑1 (MQ=255)

                                     ggATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggctg                                                      >  1:511757/1‑93 (MQ=255)

                                     ggATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggctg                                                      >  2:6149579/1‑93 (MQ=255)

                                         gcgcTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGgctggctg                                                  <  1:4965675/93‑1 (MQ=255)

                                          cgcTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGctggctgg                                                 <  1:1170172/93‑1 (MQ=255)

                                           gcTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTggg                                                >  1:5968894/1‑93 (MQ=255)

                                            cTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGc                                               >  1:3586177/1‑93 (MQ=255)

                                            cTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGc                                               >  2:6948445/1‑93 (MQ=255)

                                             ttAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCt                                              <  1:7278923/93‑1 (MQ=255)

                                              tAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCtt                                             <  1:6117441/93‑1 (MQ=255)

                                              tAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCtt                                             >  2:4237941/1‑93 (MQ=255)

                                                aGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTg                                           >  2:836043/1‑93 (MQ=255)

                                                aGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTg                                           <  2:4213350/93‑1 (MQ=255)

                                                aGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTg                                           <  2:1292277/93‑1 (MQ=255)

                                                 gCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGc                                          >  2:3495658/1‑93 (MQ=255)

                                                  cGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCt                                         <  2:4035679/93‑1 (MQ=255)

                                                    tCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTc                                       <  1:4881997/93‑1 (MQ=255)

                                                    tCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTc                                       <  2:2786707/93‑1 (MQ=255)

                                                    tCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTc                                       <  2:6059569/93‑1 (MQ=255)

                                                     cTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCg                                      <  1:4420054/93‑1 (MQ=255)

                                                     cTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCg                                      >  1:237776/1‑93 (MQ=255)

                                                      tGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCgg                                     >  2:4708256/1‑93 (MQ=255)

                                                       ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGc                                    <  2:6004163/93‑1 (MQ=255)

                                                       ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGc                                    >  1:845204/1‑93 (MQ=255)

                                                        gATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCg                                   >  2:3376318/1‑93 (MQ=255)

                                                         aTGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGa                                  <  2:5434130/93‑1 (MQ=255)

                                                           gTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGAcc                                <  2:2295738/93‑1 (MQ=255)

                                                           gTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGAcc                                >  1:2870747/1‑93 (MQ=255)

                                                           gTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCGTTGCTTCGGCGAcc                                <  2:6435919/93‑1 (MQ=255)

                                                            tCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGAccc                               >  1:4542115/1‑93 (MQ=255)

                                                              aGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGt                             >  1:6747421/1‑93 (MQ=255)

                                                               gCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTa                            >  1:1331426/1‑93 (MQ=255)

                                                                cccGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTaa                           >  1:2796487/1‑93 (MQ=255)

                                                                 ccGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAAc                          <  1:765032/93‑1 (MQ=255)

                                                                 ccGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAAc                          <  2:2387310/93‑1 (MQ=255)

                                                                  cGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACa                         <  2:3572454/93‑1 (MQ=255)

                                                                   gCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAg                        >  2:5517096/1‑93 (MQ=255)

                                                                   gCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAg                        <  2:5421175/93‑1 (MQ=255)

                                                                   gCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAg                        >  2:5304928/1‑93 (MQ=255)

                                                                    cTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGt                       >  2:2909487/1‑93 (MQ=255)

                                                                     tAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtc                      >  2:1940131/1‑93 (MQ=255)

                                                                      aGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtct                     >  1:101505/1‑93 (MQ=255)

                                                                       ggCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtctc                    <  1:6869696/93‑1 (MQ=255)

                                                                         cTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGa                  <  1:5981527/93‑1 (MQ=255)

                                                                         cTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGa                  <  2:1607322/93‑1 (MQ=255)

                                                                          tgtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGAc                 >  2:3852087/1‑93 (MQ=255)

                                                                          tgtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGAc                 <  2:6763332/93‑1 (MQ=255)

                                                                           gtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACa                <  2:5329064/93‑1 (MQ=255)

                                                                           gtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACa                <  2:2719822/93‑1 (MQ=255)

                                                                            tgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGCCAg               >  1:2096835/1‑93 (MQ=255)

                                                                             ggCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGc              <  2:1385174/93‑1 (MQ=255)

                                                                                gcgcTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGtt           <  2:6987550/93‑1 (MQ=255)

                                                                                 cgcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCCGAACAGTCTCGACAGCGttt          >  1:7050479/1‑93 (MQ=255)

                                                                                  gcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTc         >  1:5816304/1‑93 (MQ=255)

                                                                                  gcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTc         <  1:2158142/93‑1 (MQ=255)

                                                                                  gcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTc         <  2:4987083/93‑1 (MQ=255)

                                                                                  gcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTc         <  2:2627030/93‑1 (MQ=255)

                                                                                   cTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCt        >  1:5318010/1‑93 (MQ=255)

                                                                                      gCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGAc     <  1:5905447/93‑1 (MQ=255)

                                                                                        gggAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCg   <  1:5603129/93‑1 (MQ=255)

                                                                                        gggAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCg   <  2:1111642/93‑1 (MQ=255)

                                                                                         ggAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGt  >  1:5888451/1‑93 (MQ=255)

ATGTTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGT  >  NC_000913/4154943‑4155124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`