BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,136	G→A	A413A (GCG→GCA)	hydG →	HydG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,136	0	G	A	76.6%	520.4 / 135.7	249	A413A (GCG→GCA)	hydG	HydG
Reads supporting (aligned to +/- strand): ref base G (24/34); new base A (93/97); total (117/132)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 262 total aligned reads displayed.

CCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTC  >  NC_000913/4202044‑4202226

   cTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTg                                                                                         <  1:5849442/93‑1 (MQ=255)

   cTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTg                                                                                         >  1:3325326/1‑93 (MQ=255)

    tGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTgg                                                                                        <  2:5618555/93‑1 (MQ=255)

    tGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTgg                                                                                        >  1:5049262/1‑93 (MQ=255)

    tGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTgg                                                                                        <  1:2972870/93‑1 (MQ=255)

     ggCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGga                                                                                       <  2:4364581/93‑1 (MQ=255)

     ggCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGga                                                                                       <  1:6609488/93‑1 (MQ=255)

     ggCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGga                                                                                       >  2:6676679/1‑93 (MQ=255)

         aTTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGaaa                                                                                   >  1:4594810/1‑93 (MQ=255)

         aTTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGaaa                                                                                   >  2:104316/1‑93 (MQ=255)

            gCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGCGATTCTGGCGGCACTGGAGAAAACg                                                                                >  2:7001519/1‑93 (MQ=255)

             ccAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACgg                                                                               <  1:665836/93‑1 (MQ=255)

             ccAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACgg                                                                               <  2:2806501/93‑1 (MQ=255)

              cAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACggg                                                                              >  1:2656712/1‑93 (MQ=255)

              cAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACggg                                                                              <  1:4356061/93‑1 (MQ=255)

               aGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTTGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggc                                                                             <  2:7368403/93‑1 (MQ=255)

               aGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggc                                                                             <  2:3904584/93‑1 (MQ=255)

                gTAGGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggcg                                                                            <  1:1754248/93‑1 (MQ=255)

                gTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggcg                                                                            >  2:5222607/1‑93 (MQ=255)

                gTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggcg                                                                            >  2:5550718/1‑93 (MQ=255)

                gTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggcg                                                                            <  2:5361496/93‑1 (MQ=255)

                gTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGggcg                                                                            >  2:5479562/1‑93 (MQ=255)

                   cGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGca                                                                         >  1:1502369/1‑93 (MQ=255)

                    gCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGcaa                                                                        >  1:4056304/1‑93 (MQ=255)

                    gCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGcaa                                                                        >  1:783701/1‑93 (MQ=255)

                       gATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGcaacaa                                                                     >  2:7284762/1‑93 (MQ=255)

                       gATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGcaacaa                                                                     >  2:6060900/1‑93 (MQ=255)

                        aTCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACaaa                                                                    >  2:1822582/1‑93 (MQ=255)

                        aTCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACaaa                                                                    >  1:2403540/1‑93 (MQ=255)

                        aTCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACaaa                                                                    >  2:1898621/1‑93 (MQ=255)

                         tCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACaaaa                                                                   >  1:6201506/1‑93 (MQ=255)

                         tCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACaaaa                                                                   <  1:1351642/93‑1 (MQ=255)

                          cccGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAAc                                                                  >  2:6778855/1‑93 (MQ=255)

                             gCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGa                                                               <  2:1630527/93‑1 (MQ=255)

                              cTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGaa                                                              <  1:5511060/93‑1 (MQ=255)

                              cTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGaa                                                              <  2:4515458/93‑1 (MQ=255)

                               tGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAg                                                             >  2:3833328/1‑93 (MQ=255)

                               tGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAg                                                             <  2:2774910/93‑1 (MQ=255)

                               tGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAg                                                             >  1:7468052/1‑93 (MQ=255)

                               tGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAg                                                             >  1:7268713/1‑93 (MQ=255)

                                 ggACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAgcc                                                           <  2:6500216/93‑1 (MQ=255)

                                  gACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAgccg                                                          >  1:1631786/1‑93 (MQ=255)

                                    cAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAgccgcc                                                        <  1:5295895/93‑1 (MQ=255)

                                    cAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAgccgcc                                                        >  2:4468721/1‑93 (MQ=255)

                                     aaaGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGccc                                                       >  2:1350851/1‑93 (MQ=255)

                                     aaaGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGccc                                                       >  1:7220868/1‑93 (MQ=255)

                                          cAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAg                                                  <  2:7462291/93‑1 (MQ=255)

                                           aGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGt                                                 >  2:531484/1‑93 (MQ=255)

                                            ggATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGtt                                                >  1:3793843/1‑93 (MQ=255)

                                            ggATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGtt                                                <  2:4098990/93‑1 (MQ=255)

                                             gATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTa                                               <  1:6297053/93‑1 (MQ=255)

                                             gATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTa                                               >  2:6282270/1‑93 (MQ=255)

                                             gATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTa                                               <  2:5061129/93‑1 (MQ=255)

                                               tatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAgg                                             >  1:5169322/1‑93 (MQ=255)

                                                 ttCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGa                                           <  2:3275239/93‑1 (MQ=255)

                                                 ttCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGa                                           >  2:4978635/1‑93 (MQ=255)

                                                  tCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGAt                                          >  1:1433252/1‑93 (MQ=255)

                                                    aGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCa                                        <  2:3529895/93‑1 (MQ=255)

                                                    aGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCa                                        <  2:5965444/93‑1 (MQ=255)

                                                     gCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAc                                       <  2:5741692/93‑1 (MQ=255)

                                                     gCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAc                                       <  2:2506718/93‑1 (MQ=255)

                                                     gCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAc                                       >  2:110710/1‑93 (MQ=255)

                                                      ccGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCAcg                                      <  2:2865534/93‑1 (MQ=255)

                                                       cGTTGGTGGAAGAGGAAAAAGAGGTGATTCTGGCGGCTCTGGAGAAAACGGGGGGCAAAAAAACCGAAGCCGCCCGTCAGTTAGGGATCAcgc                                     <  1:6066073/93‑1 (MQ=255)

                                                            gtggaagtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCaaa                                >  2:2036146/1‑93 (MQ=255)

                                                             tggaagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCaaaa                               <  2:2282161/93‑1 (MQ=255)

                                                             tggaagtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCaaaa                               <  1:5833878/93‑1 (MQ=255)

                                                             tggaagtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCaaaa                               <  2:4732308/93‑1 (MQ=255)

                                                                 agtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCt                           >  2:3037857/1‑93 (MQ=255)

                                                                 agtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCt                           >  1:3133879/1‑93 (MQ=255)

                                                                  gtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTa                          >  1:2492218/1‑93 (MQ=255)

                                                                  gtggaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTa                          <  2:4842682/93‑1 (MQ=255)

                                                                     gaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTg                       >  1:6616043/1‑93 (MQ=255)

                                                                     gaaAAAGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTg                       <  1:1021745/93‑1 (MQ=255)

                                                                      aaaaaGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTgg                      <  1:3884793/93‑1 (MQ=255)

                                                                        aaaGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCa                    >  2:5653142/1‑93 (MQ=255)

                                                                        aaaGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCa                    >  1:7305907/1‑93 (MQ=255)

                                                                         aaGAGGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCaa                   >  2:6566302/1‑93 (MQ=255)

                                                                             ggTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACt               <  1:2285259/93‑1 (MQ=255)

                                                                              gTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTg              >  1:5180069/1‑93 (MQ=255)

                                                                              gTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTg              <  1:5858480/93‑1 (MQ=255)

                                                                               tGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGt             <  1:1140944/93‑1 (MQ=255)

                                                                               tGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGt             <  1:6817771/93‑1 (MQ=255)

                                                                               tGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGt             <  1:3530121/93‑1 (MQ=255)

                                                                                gATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTc            >  1:1985785/1‑93 (MQ=255)

                                                                                gATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTc            <  2:1959795/93‑1 (MQ=255)

                                                                                gATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTc            <  1:7528540/93‑1 (MQ=255)

                                                                                gATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTc            <  1:3018020/93‑1 (MQ=255)

                                                                                  ttCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTcgc          >  1:3991380/1‑93 (MQ=255)

                                                                                  ttCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTcgc          <  1:4447183/93‑1 (MQ=255)

                                                                                    cTGGCGGCACTGGAGAAAACTGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGt        >  2:4601392/1‑93 (MQ=255)

                                                                                     tGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGtt       >  1:2044523/1‑93 (MQ=255)

                                                                                     tGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGtt       <  2:6184054/93‑1 (MQ=255)

                                                                                     tGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGtt       >  1:5681708/1‑93 (MQ=255)

                                                                                      ggcggcACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTa      <  1:4783532/93‑1 (MQ=255)

                                                                                       gcggcaCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAg     <  1:7085629/93‑1 (MQ=255)

                                                                                        cggcgCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGt    <  1:7109011/93‑1 (MQ=255)

                                                                                        cggcaCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGt    <  2:42973/93‑1 (MQ=255)

                                                                                         ggcaCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGtt   >  1:4434446/1‑93 (MQ=255)

                                                                                         ggcaCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGtt   <  1:6703348/93‑1 (MQ=255)

CCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTC  >  NC_000913/4202044‑4202226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`