BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,798	A→G	H270R (CAC→CGC)	mutL →	MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,798	0	A	G	84.9%	576.9 / 95.1	252	H270R (CAC→CGC)	mutL	MutL
Reads supporting (aligned to +/- strand): ref base A (19/19); new base G (97/117); total (116/136)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-02

Only 100 of 271 total aligned reads displayed.

TGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGA  >  NC_000913/4395708‑4395888

tGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATcgcg                                                                                          <  2:2861852/93‑1 (MQ=255)

    cGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGAt                                                                                      <  1:6517079/93‑1 (MQ=255)

      aTCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATcc                                                                                    >  2:764494/1‑93 (MQ=255)

      aTCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATcc                                                                                    <  2:3649048/93‑1 (MQ=255)

      aTCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATcc                                                                                    <  2:1565734/93‑1 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGc                                                                                  >  2:3478220/1‑93 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGc                                                                                  >  1:6959288/1‑93 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGc                                                                                  <  2:6948730/93‑1 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGc                                                                                  <  2:2006977/93‑1 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGc                                                                                  >  1:246283/1‑93 (MQ=255)

         cAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGcc                                                                                 <  1:3895492/93‑1 (MQ=255)

         cAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGcc                                                                                 <  2:5609740/93‑1 (MQ=255)

          aaaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCa                                                                                <  2:522460/93‑1 (MQ=255)

           aaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAg                                                                               <  1:171048/93‑1 (MQ=255)

           aaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAg                                                                               <  1:6054964/93‑1 (MQ=255)

             tCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGc                                                                             >  2:3361043/1‑93 (MQ=255)

             tCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCACCAGGc                                                                             <  2:1916942/93‑1 (MQ=255)

                caccacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTg                                                                          >  1:6144404/1‑93 (MQ=255)

                 accacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGc                                                                         >  1:5764053/1‑93 (MQ=255)

                 accacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGc                                                                         >  1:1467993/1‑93 (MQ=255)

                 accacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGc                                                                         <  2:5060885/93‑1 (MQ=255)

                 accacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGc                                                                         <  1:747510/93‑1 (MQ=255)

                   cacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGa                                                                       >  2:4525272/1‑93 (MQ=255)

                    acGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGaa                                                                      <  2:1760381/93‑1 (MQ=255)

                     cGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAg                                                                     >  2:4316976/1‑93 (MQ=255)

                      gCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGa                                                                    >  1:6593522/1‑93 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                   >  1:3949623/1‑93 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                   <  2:139880/93‑1 (MQ=255)

                        ccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACa                                                                  >  2:6796860/1‑93 (MQ=255)

                        ccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACa                                                                  >  2:6589858/1‑93 (MQ=255)

                        ccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACa                                                                  >  1:3119735/1‑93 (MQ=255)

                         cGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaa                                                                 >  1:4459640/1‑93 (MQ=255)

                          gCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaaa                                                                <  1:913938/93‑1 (MQ=255)

                          gCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaaa                                                                <  2:4190329/93‑1 (MQ=255)

                          gCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaaa                                                                <  2:6621665/93‑1 (MQ=255)

                          gCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaaa                                                                <  1:1230884/93‑1 (MQ=255)

                            aCTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACt                                                              >  2:2086388/1‑93 (MQ=255)

                             cTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTg                                                             >  1:5915803/1‑93 (MQ=255)

                             cTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTg                                                             >  1:5523130/1‑93 (MQ=255)

                             cTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTg                                                             >  1:1586064/1‑93 (MQ=255)

                             cTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTg                                                             >  1:4357597/1‑93 (MQ=255)

                              tGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTgg                                                            <  1:3211236/93‑1 (MQ=255)

                                gCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTgggg                                                          <  1:1990850/93‑1 (MQ=255)

                                gCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTgggg                                                          <  2:7195158/93‑1 (MQ=255)

                                gCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCCAAGACAAACTgggg                                                          <  2:6442125/93‑1 (MQ=255)

                                 cAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTggggg                                                         >  1:3541208/1‑93 (MQ=255)

                                  aGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGc                                                        >  2:5282099/1‑93 (MQ=255)

                                  aGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGc                                                        <  2:3781588/93‑1 (MQ=255)

                                  aGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGc                                                        <  1:2694048/93‑1 (MQ=255)

                                   gAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGcc                                                       >  1:5993638/1‑93 (MQ=255)

                                   gAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGcc                                                       >  1:5274398/1‑93 (MQ=255)

                                      aTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAt                                                    <  2:2271499/93‑1 (MQ=255)

                                      aTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAt                                                    <  1:5625286/93‑1 (MQ=255)

                                       ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATc                                                   >  2:2724225/1‑93 (MQ=255)

                                       ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATc                                                   <  1:76947/93‑1 (MQ=255)

                                        tCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCa                                                  >  1:88546/1‑93 (MQ=255)

                                        tCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCa                                                  <  2:5421452/93‑1 (MQ=255)

                                          aGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGc                                                >  1:2037893/1‑93 (MQ=255)

                                           gTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCa                                               <  2:2971192/93‑1 (MQ=255)

                                            tATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCaa                                              >  2:2433565/1‑93 (MQ=255)

                                             aTTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAc                                             <  2:6731398/93‑1 (MQ=255)

                                              ttGCTACGTGAACTGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAcc                                            <  2:1105475/93‑1 (MQ=255)

                                              ttGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAcc                                            >  1:2992173/1‑93 (MQ=255)

                                              ttGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAcc                                            >  1:3604647/1‑93 (MQ=255)

                                                 cTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGc                                         <  2:6491098/93‑1 (MQ=255)

                                                  tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCa                                        <  2:7285124/93‑1 (MQ=255)

                                                  tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCa                                        >  2:5529124/1‑93 (MQ=255)

                                                   aCGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAt                                       <  1:215575/93‑1 (MQ=255)

                                                    cGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAtt                                      <  2:4973670/93‑1 (MQ=255)

                                                      tGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTtg                                    >  1:1119856/1‑93 (MQ=255)

                                                       gAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTtgt                                   <  2:3127604/93‑1 (MQ=255)

                                                        aaCGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTtgtg                                  <  1:7247699/93‑1 (MQ=255)

                                                        aaCGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTtgtg                                  <  2:3578821/93‑1 (MQ=255)

                                                           ggTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGtgttg                               <  2:91024/93‑1 (MQ=255)

                                                             tCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTa                             <  1:6756918/93‑1 (MQ=255)

                                                              cGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAt                            <  2:3172434/93‑1 (MQ=255)

                                                               gCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                           <  2:2507933/93‑1 (MQ=255)

                                                                cATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCt                          >  2:327678/1‑93 (MQ=255)

                                                                 atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTg                         >  1:3324821/1‑93 (MQ=255)

                                                                 atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTg                         <  2:1164308/93‑1 (MQ=255)

                                                                  tgatgCGCGATTGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTgg                        >  1:4869383/1‑93 (MQ=255)

                                                                  tgatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTgg                        >  1:1007138/1‑93 (MQ=255)

                                                                  tgatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTgg                        >  1:2225724/1‑93 (MQ=255)

                                                                   gatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGga                       <  1:7161537/93‑1 (MQ=255)

                                                                     tgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGgaga                     >  2:1299513/1‑93 (MQ=255)

                                                                        gcgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCg                  >  2:4692401/1‑93 (MQ=255)

                                                                        gcgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCg                  <  1:6869493/93‑1 (MQ=255)

                                                                             cGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCca             <  2:5582110/93‑1 (MQ=255)

                                                                              gCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCcac            >  2:1709278/1‑93 (MQ=255)

                                                                               ccTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCcaca           >  2:2180619/1‑93 (MQ=255)

                                                                                cTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACAt          >  2:5859704/1‑93 (MQ=255)

                                                                                 tGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc         <  1:4193643/93‑1 (MQ=255)

                                                                                  gATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa        >  2:1624297/1‑93 (MQ=255)

                                                                                  gATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa        <  1:670944/93‑1 (MQ=255)

                                                                                  gATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa        <  1:2488353/93‑1 (MQ=255)

                                                                                  gATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa        <  1:7137352/93‑1 (MQ=255)

                                                                                    tcaatcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAgg      <  2:406812/93‑1 (MQ=255)

                                                                                    tcaatcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAgg      <  2:2828303/93‑1 (MQ=255)

                                                                                    tcaatcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAgg      >  1:4767949/1‑93 (MQ=255)

                                                                                        tcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGa  <  2:5305588/93‑1 (MQ=255)

TGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGA  >  NC_000913/4395708‑4395888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`