Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,404,526 C→T L99L (CTG→TTG)  b4179 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,404,5260CT89.1% 1110.2 / 77.1 357L99L (CTG→TTG) b4179hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (15/24);  new base T (136/182);  total (151/206)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 363 total aligned reads displayed.

TGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTG  >  NC_000913/4404434‑4404618
                                                                                            |                                                                                            
    cTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCg                                                                                          >  2:1556988/1‑93 (MQ=255)
     ttCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCgg                                                                                         <  1:3167181/93‑1 (MQ=255)
       cACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGt                                                                                       <  2:3671959/93‑1 (MQ=255)
       cACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGt                                                                                       >  2:6654254/1‑93 (MQ=255)
       cACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGt                                                                                       <  2:440515/93‑1 (MQ=255)
        aCTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGtt                                                                                      <  2:174092/93‑1 (MQ=255)
        aCTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGtt                                                                                      <  2:6117888/93‑1 (MQ=255)
        aCTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGtt                                                                                      <  1:5498557/93‑1 (MQ=255)
            gtcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAg                                                                                  <  1:5379067/93‑1 (MQ=255)
            gtcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAg                                                                                  <  2:2511621/93‑1 (MQ=255)
            gtcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAg                                                                                  <  1:2999010/93‑1 (MQ=255)
             tcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAgg                                                                                 <  1:4644739/93‑1 (MQ=255)
             tcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAgg                                                                                 >  1:1935423/1‑93 (MQ=255)
             tcgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAgg                                                                                 <  2:4133361/93‑1 (MQ=255)
               gtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGc                                                                               >  2:7063428/1‑93 (MQ=255)
                tcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCg                                                                              >  1:1454503/1‑93 (MQ=255)
                 cAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGt                                                                             <  2:225586/93‑1 (MQ=255)
                   gTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTaa                                                                           <  2:5646512/93‑1 (MQ=255)
                    tGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTaaa                                                                          >  2:5759068/1‑93 (MQ=255)
                    tGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTaaa                                                                          >  1:4739059/1‑93 (MQ=255)
                    tGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTaaa                                                                          >  2:2781096/1‑93 (MQ=255)
                       tATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAgat                                                                       >  1:4442172/1‑93 (MQ=255)
                       tATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAgat                                                                       <  2:3359178/93‑1 (MQ=255)
                         tGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAgatga                                                                     >  2:1944755/1‑93 (MQ=255)
                         tGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAgatga                                                                     <  2:2091291/93‑1 (MQ=255)
                          gcgcTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAgatgat                                                                    <  1:5958217/93‑1 (MQ=255)
                            gcTNCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGAttt                                                                  <  1:4832388/93‑1 (MQ=255)
                            gcTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGAttt                                                                  <  2:2600132/93‑1 (MQ=255)
                              tGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGt                                                                <  2:2388366/93‑1 (MQ=255)
                               gCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTa                                                               <  2:4711796/93‑1 (MQ=255)
                               gCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTa                                                               <  1:1831176/93‑1 (MQ=255)
                               gCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTa                                                               <  2:3831666/93‑1 (MQ=255)
                                 cGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtc                                                             <  1:846772/93‑1 (MQ=255)
                                 cGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtc                                                             >  2:4464571/1‑93 (MQ=255)
                                  ggAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtct                                                            >  1:5594606/1‑93 (MQ=255)
                                  ggAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtct                                                            >  2:6894717/1‑93 (MQ=255)
                                  ggAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtct                                                            >  2:5269210/1‑93 (MQ=255)
                                   gAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtctc                                                           <  2:6296482/93‑1 (MQ=255)
                                    aaCGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtctct                                                          <  1:6932325/93‑1 (MQ=255)
                                     aCGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtctctc                                                         <  2:4948439/93‑1 (MQ=255)
                                     aCGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTAtctctc                                                         >  1:3975577/1‑93 (MQ=255)
                                      cGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTcc                                                        <  2:1361834/93‑1 (MQ=255)
                                      cGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTcc                                                        <  1:1249022/93‑1 (MQ=255)
                                       gCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCa                                                       <  2:5369768/93‑1 (MQ=255)
                                        ccTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAg                                                      >  1:6749732/1‑93 (MQ=255)
                                        ccTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAg                                                      >  2:3520530/1‑93 (MQ=255)
                                          tCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCg                                                    >  1:2433764/1‑93 (MQ=255)
                                           cGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGa                                                   <  2:5507454/93‑1 (MQ=255)
                                             aCCTGGTGAAAGGTACCGTTATTCGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGc                                                 >  1:3579904/1‑93 (MQ=255)
                                              ccTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCa                                                >  2:5029392/1‑93 (MQ=255)
                                              ccTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCa                                                <  2:6230240/93‑1 (MQ=255)
                                               cTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAg                                               >  1:7481394/1‑93 (MQ=255)
                                               cTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAg                                               >  2:3975044/1‑93 (MQ=255)
                                                tGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGa                                              <  2:5135196/93‑1 (MQ=255)
                                                tGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGa                                              >  2:1209928/1‑93 (MQ=255)
                                                tGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGa                                              <  1:2860395/93‑1 (MQ=255)
                                                 ggTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGAt                                             <  2:2967725/93‑1 (MQ=255)
                                                  gTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATg                                            <  1:5669958/93‑1 (MQ=255)
                                                  gTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATg                                            >  2:2166430/1‑93 (MQ=255)
                                                  gTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATg                                            <  1:142005/93‑1 (MQ=255)
                                                    gAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGaa                                          <  1:7073214/93‑1 (MQ=255)
                                                     aaaGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGaaa                                         >  1:6570658/1‑93 (MQ=255)
                                                      aaGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGaaaa                                        <  2:758464/93‑1 (MQ=255)
                                                      aaGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGaaaa                                        <  1:6132413/93‑1 (MQ=255)
                                                      aaGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGaaaa                                        <  1:2494963/93‑1 (MQ=255)
                                                        ggTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAAcc                                      >  2:2890476/1‑93 (MQ=255)
                                                        ggTACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAAcc                                      <  1:6401873/93‑1 (MQ=255)
                                                          tACCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTg                                    <  2:325488/93‑1 (MQ=255)
                                                           aCCGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGc                                   >  2:1406058/1‑93 (MQ=255)
                                                            ccGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCa                                  >  2:1627696/1‑93 (MQ=255)
                                                            ccGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCa                                  <  2:6628617/93‑1 (MQ=255)
                                                            ccGTTATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCa                                  >  1:3345844/1‑93 (MQ=255)
                                                               ttATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTc                               >  2:1303937/1‑93 (MQ=255)
                                                                tATTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCa                              <  2:3217921/93‑1 (MQ=255)
                                                                 aTTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCAt                             <  1:3510244/93‑1 (MQ=255)
                                                                 aTTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCAt                             <  1:6532608/93‑1 (MQ=255)
                                                                 aTTGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCAt                             <  2:7144954/93‑1 (MQ=255)
                                                                  ttGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATg                            <  2:6543379/93‑1 (MQ=255)
                                                                  ttGGCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATg                            <  1:6255130/93‑1 (MQ=255)
                                                                     gCCACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCg                         <  1:1674193/93‑1 (MQ=255)
                                                                      ccACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGa                        >  2:2118473/1‑93 (MQ=255)
                                                                      ccACCGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGa                        <  1:7201779/93‑1 (MQ=255)
                                                                         ccGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGTATTCATGGCGATCa                     <  2:6437772/93‑1 (MQ=255)
                                                                         ccGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCa                     >  1:1146785/1‑93 (MQ=255)
                                                                         ccGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCa                     <  1:4115003/93‑1 (MQ=255)
                                                                          cGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAg                    <  1:7367948/93‑1 (MQ=255)
                                                                          cGTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAg                    >  2:1021294/1‑93 (MQ=255)
                                                                           gTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAgg                   <  1:325886/93‑1 (MQ=255)
                                                                           gTGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAgg                   <  1:210099/93‑1 (MQ=255)
                                                                           gTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAgg                   >  2:3437717/1‑93 (MQ=255)
                                                                            tGATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGt                  <  1:5133835/93‑1 (MQ=255)
                                                                             gATGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTg                 <  2:6142550/93‑1 (MQ=255)
                                                                              aTGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGc                <  2:5068782/93‑1 (MQ=255)
                                                                               tGGCTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCt               <  1:6439673/93‑1 (MQ=255)
                                                                                  cTACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGc            >  1:5009369/1‑93 (MQ=255)
                                                                                   tACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCt           <  1:3734295/93‑1 (MQ=255)
                                                                                   tACGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCt           >  1:5245741/1‑93 (MQ=255)
                                                                                    aCGGCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTc          >  1:3909562/1‑93 (MQ=255)
                                                                                      ggCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAg        >  2:3075058/1‑93 (MQ=255)
                                                                                       gCTTTTTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGc       <  2:477387/93‑1 (MQ=255)
                                                                                            |                                                                                            
TGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTG  >  NC_000913/4404434‑4404618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑