BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,677	A→G	G108G (GGT→GGC)	yjfZ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,677	0	A	G	84.6%	669.8 / 114.8	293	G108G (GGT→GGC)	yjfZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (27/18); new base G (125/123); total (152/141)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 301 total aligned reads displayed.

CCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATTGTGAT  >  NC_000913/4424585‑4424769

cccAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCa                                                                                              >  1:1266124/1‑93 (MQ=255)

 ccAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCAc                                                                                             >  2:3365026/1‑93 (MQ=255)

  cAAACCCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATTTTTCAGTTCTGCGGTTAACCTCGCAcc                                                                                            <  1:7478719/93‑1 (MQ=255)

  cAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGcc                                                                                            <  2:3941682/93‑1 (MQ=255)

   aaaaCCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGccc                                                                                           >  2:7000183/1‑93 (MQ=255)

    aaaCCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCcg                                                                                          >  2:6324841/1‑93 (MQ=255)

        cACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGCTCTGCGGTTAACCTCGCGCCCGCGTa                                                                                      >  2:5436386/1‑93 (MQ=255)

         aCTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTaa                                                                                     >  2:7497299/1‑93 (MQ=255)

         aCTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTaa                                                                                     <  2:2737196/93‑1 (MQ=255)

         aCTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTaa                                                                                     <  1:6010530/93‑1 (MQ=255)

            tCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCt                                                                                  <  1:817596/93‑1 (MQ=255)

              gtgtattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCttt                                                                                >  1:7302652/1‑93 (MQ=255)

              gtgtattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCttt                                                                                >  1:3810639/1‑93 (MQ=255)

               tgtattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTa                                                                               <  2:4345277/93‑1 (MQ=255)

               tgtattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTa                                                                               >  2:2968995/1‑93 (MQ=255)

                gtattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTAt                                                                              >  1:5082697/1‑93 (MQ=255)

                 tattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTAtt                                                                             <  2:6561875/93‑1 (MQ=255)

                 tattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTAtt                                                                             <  2:4142891/93‑1 (MQ=255)

                 tattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTAtt                                                                             <  2:2004830/93‑1 (MQ=255)

                 tattgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTAtt                                                                             >  1:39881/1‑93 (MQ=255)

                  attgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTg                                                                            >  1:4111941/1‑93 (MQ=255)

                   ttgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGt                                                                           <  2:4120773/93‑1 (MQ=255)

                   ttgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGt                                                                           >  1:5555149/1‑93 (MQ=255)

                    tgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTa                                                                          <  1:6530823/93‑1 (MQ=255)

                    tgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTa                                                                          >  2:4553830/1‑93 (MQ=255)

                    tgtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTa                                                                          <  2:1458910/93‑1 (MQ=255)

                     gtgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTAt                                                                         >  1:4561032/1‑93 (MQ=255)

                      tgtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTAtt                                                                        >  2:4517702/1‑93 (MQ=255)

                       gtattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTAttt                                                                       >  2:5132083/1‑93 (MQ=255)

                        tattTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTc                                                                      <  2:4013509/93‑1 (MQ=255)

                         attTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCt                                                                     >  1:7255420/1‑93 (MQ=255)

                         attTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCt                                                                     >  2:5281621/1‑93 (MQ=255)

                         attTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCt                                                                     >  2:3253083/1‑93 (MQ=255)

                          tttACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTg                                                                    <  1:1887445/93‑1 (MQ=255)

                           ttACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTgg                                                                   <  1:3437017/93‑1 (MQ=255)

                           ttACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTgg                                                                   <  1:3345192/93‑1 (MQ=255)

                           ttACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTgg                                                                   <  2:7388362/93‑1 (MQ=255)

                           ttACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTgg                                                                   <  1:6657868/93‑1 (MQ=255)

                             aCTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGc                                                                 >  1:2440454/1‑93 (MQ=255)

                             aCTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGc                                                                 >  1:5248401/1‑93 (MQ=255)

                                 ttGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGa                                                             <  2:6775959/93‑1 (MQ=255)

                                  tGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGaa                                                            <  2:3551983/93‑1 (MQ=255)

                                    ggTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAata                                                          <  1:1570253/93‑1 (MQ=255)

                                      ttCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAAtatt                                                        >  2:4228814/1‑93 (MQ=255)

                                       tCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAAtatta                                                       >  1:3200535/1‑93 (MQ=255)

                                        cGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAAtattat                                                      <  1:4878077/93‑1 (MQ=255)

                                        cGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAAtattat                                                      >  2:1268014/1‑93 (MQ=255)

                                         gAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATc                                                     >  1:6355624/1‑93 (MQ=255)

                                          aaGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCt                                                    <  2:3680271/93‑1 (MQ=255)

                                            ggATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTaa                                                  <  1:4309671/93‑1 (MQ=255)

                                                tCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTa                                              <  2:7099100/93‑1 (MQ=255)

                                                tCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTa                                              <  1:779190/93‑1 (MQ=255)

                                                 cGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAg                                             >  1:5845733/1‑93 (MQ=255)

                                                 cGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCGCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAg                                             >  1:6222295/1‑93 (MQ=255)

                                                   aCATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCt                                           >  2:6931198/1‑93 (MQ=255)

                                                     aTCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTc                                         >  1:3382176/1‑93 (MQ=255)

                                                     aTCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTc                                         >  1:6716999/1‑93 (MQ=255)

                                                      tCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCa                                        >  2:374906/1‑93 (MQ=255)

                                                      tCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCa                                        <  1:7167572/93‑1 (MQ=255)

                                                       cTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCAt                                       <  1:4766782/93‑1 (MQ=255)

                                                        ttGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATg                                      >  2:5859024/1‑93 (MQ=255)

                                                        ttGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATg                                      <  1:7290233/93‑1 (MQ=255)

                                                         tGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATgg                                     <  1:6892766/93‑1 (MQ=255)

                                                          gCAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGt                                    <  1:2742184/93‑1 (MQ=255)

                                                           cAATAAATATTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGtt                                   >  2:4899255/1‑93 (MQ=255)

                                                            aataaataTTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtc                                  >  1:890261/1‑93 (MQ=255)

                                                              taaataTTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtcca                                <  1:2056634/93‑1 (MQ=255)

                                                              taaataTTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtcca                                <  1:166884/93‑1 (MQ=255)

                                                              taaataTTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtcca                                >  2:6660815/1‑93 (MQ=255)

                                                                aataTTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccatc                              >  2:6886482/1‑93 (MQ=255)

                                                                 atatTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccatcc                             <  1:7100226/93‑1 (MQ=255)

                                                                  tatTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccatcca                            >  1:741493/1‑93 (MQ=255)

                                                                   atTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCaa                           <  1:2569875/93‑1 (MQ=255)

                                                                   atTTCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCaa                           <  1:3059799/93‑1 (MQ=255)

                                                                    tttCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCaaa                          >  2:1802789/1‑93 (MQ=255)

                                                                     ttCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCaaaa                         <  2:1674581/93‑1 (MQ=255)

                                                                      tCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAg                        >  1:6441638/1‑93 (MQ=255)

                                                                      tCAGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAg                        >  1:3715047/1‑93 (MQ=255)

                                                                        aGTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGaa                      <  1:2214047/93‑1 (MQ=255)

                                                                         gTTCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAAt                     >  1:986432/1‑93 (MQ=255)

                                                                          ttCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAAtt                    >  1:3228146/1‑93 (MQ=255)

                                                                          ttCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAAtt                    <  1:2161627/93‑1 (MQ=255)

                                                                          ttCTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAAtt                    <  2:2482761/93‑1 (MQ=255)

                                                                            cTGCGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTAt                  >  2:3431876/1‑93 (MQ=255)

                                                                              gCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCa                >  2:3822154/1‑93 (MQ=255)

                                                                               cGGTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCAc               <  1:1113112/93‑1 (MQ=255)

                                                                                ggTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCAcc              <  2:114858/93‑1 (MQ=255)

                                                                                ggTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCAcc              >  2:6012256/1‑93 (MQ=255)

                                                                                 gTTAACCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACca             >  2:2153826/1‑93 (MQ=255)

                                                                                    aaCCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACcattc          <  1:2331665/93‑1 (MQ=255)

                                                                                     aCCTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACcattca         >  1:5019211/1‑93 (MQ=255)

                                                                                      ccTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACcattcat        >  2:924536/1‑93 (MQ=255)

                                                                                      ccTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACcattcat        <  2:7151077/93‑1 (MQ=255)

                                                                                      ccTCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACcattcat        >  1:2960629/1‑93 (MQ=255)

                                                                                        tCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATtg      >  1:699277/1‑93 (MQ=255)

                                                                                        tCGCGCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATtg      >  1:3776221/1‑93 (MQ=255)

                                                                                         cgcgCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATtgt     <  2:6169681/93‑1 (MQ=255)

                                                                                         cgcgCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATtgt     <  1:5704312/93‑1 (MQ=255)

                                                                                         cgcgCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATtgt     <  2:5678801/93‑1 (MQ=255)

                                                                                            aCCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATTGTGAt  >  2:1884108/1‑93 (MQ=255)

CCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCAAAAGAATTATCACCATTCATTGTGAT  >  NC_000913/4424585‑4424769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`