BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,522,805	G→A	A189A (GCC→GCT)	yjhI ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,522,805	0	G	A	80.9%	683.3 / 118.6	277	A189A (GCC→GCT)	yjhI	putative regulator
Reads supporting (aligned to +/- strand): ref base G (30/23); new base A (102/122); total (132/145)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 285 total aligned reads displayed.

GAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGC  >  NC_000913/4522713‑4522897

  aaGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGc                                                                                            <  1:7034369/93‑1 (MQ=255)

  aaGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGc                                                                                            <  1:3694498/93‑1 (MQ=255)

   aGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGcc                                                                                           <  1:194173/93‑1 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGccc                                                                                          >  2:7229189/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGccc                                                                                          >  2:6785437/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGccc                                                                                          >  2:596427/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGccc                                                                                          >  1:4284343/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGccc                                                                                          >  2:1753441/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGccc                                                                                          >  1:609480/1‑93 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGccc                                                                                          <  2:5430831/93‑1 (MQ=255)

    gTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGccc                                                                                          >  1:1350025/1‑93 (MQ=255)

     tGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCa                                                                                         <  2:2900121/93‑1 (MQ=255)

     tGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCa                                                                                         <  1:33730/93‑1 (MQ=255)

       agaTTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATc                                                                                       <  1:2216390/93‑1 (MQ=255)

       agaTTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATc                                                                                       >  2:4276646/1‑93 (MQ=255)

       agaTTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATc                                                                                       <  2:3453287/93‑1 (MQ=255)

       agaTTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATc                                                                                       <  1:5844798/93‑1 (MQ=255)

       agaTTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATc                                                                                       >  1:3177052/1‑93 (MQ=255)

           tGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCg                                                                                   >  2:6091856/1‑93 (MQ=255)

            gCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGa                                                                                  <  2:793224/93‑1 (MQ=255)

            gCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGa                                                                                  >  1:3245705/1‑93 (MQ=255)

            gCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGa                                                                                  >  1:3039295/1‑93 (MQ=255)

             cATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGaa                                                                                 >  1:7193772/1‑93 (MQ=255)

              aTAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAg                                                                                >  1:6572630/1‑93 (MQ=255)

               tAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAgg                                                                               <  1:6283722/93‑1 (MQ=255)

               tAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAgg                                                                               >  1:1288706/1‑93 (MQ=255)

               tAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAgg                                                                               >  1:5687380/1‑93 (MQ=255)

               tAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAgg                                                                               >  1:4759450/1‑93 (MQ=255)

                aGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGc                                                                              >  1:2884234/1‑93 (MQ=255)

                 gTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCg                                                                             >  1:6652186/1‑93 (MQ=255)

                   tAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCgtg                                                                           <  1:1118656/93‑1 (MQ=255)

                    aaCTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCgtgt                                                                          <  1:6927863/93‑1 (MQ=255)

                    aaCTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCgtgt                                                                          <  2:6059925/93‑1 (MQ=255)

                       tctGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGtttt                                                                       >  2:401878/1‑93 (MQ=255)

                       tctGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGtttt                                                                       <  2:3420344/93‑1 (MQ=255)

                       tctGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGtttt                                                                       <  1:554131/93‑1 (MQ=255)

                        ctGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGttttt                                                                      <  1:1075572/93‑1 (MQ=255)

                             ataCATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTaa                                                                 >  1:6085954/1‑93 (MQ=255)

                              taCATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAAt                                                                <  1:4996213/93‑1 (MQ=255)

                              taCATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAt                                                                <  2:117427/93‑1 (MQ=255)

                                cATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAAttc                                                              <  2:6711844/93‑1 (MQ=255)

                                cATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTCGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttc                                                              <  1:3839575/93‑1 (MQ=255)

                                 atatTGAATACCGGCATACTTAAACATGCCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttct                                                             >  2:6428159/1‑93 (MQ=255)

                                 atatTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttct                                                             >  1:2833368/1‑93 (MQ=255)

                                  tatTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttctt                                                            >  1:4012087/1‑93 (MQ=255)

                                  tatTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttctt                                                            <  1:5366615/93‑1 (MQ=255)

                                   atTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAAttcttc                                                           <  2:3863137/93‑1 (MQ=255)

                                    ttGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTcc                                                          <  1:5901837/93‑1 (MQ=255)

                                     tGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCa                                                         >  1:5486226/1‑93 (MQ=255)

                                         tACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGaaa                                                     <  2:1086433/93‑1 (MQ=255)

                                          aCCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAAc                                                    <  2:2190214/93‑1 (MQ=255)

                                          aCCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAAc                                                    <  2:3935326/93‑1 (MQ=255)

                                            cGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACaa                                                  <  2:2037394/93‑1 (MQ=255)

                                            cGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACaa                                                  >  2:2101947/1‑93 (MQ=255)

                                             ggCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAt                                                 <  1:5198380/93‑1 (MQ=255)

                                              gCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtt                                                <  2:4340101/93‑1 (MQ=255)

                                              gCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtt                                                >  1:4590977/1‑93 (MQ=255)

                                                aTACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtttt                                              >  2:4595227/1‑93 (MQ=255)

                                                aTACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtttt                                              <  2:4158015/93‑1 (MQ=255)

                                                 tACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAttttt                                             >  1:3555240/1‑93 (MQ=255)

                                                  aCTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtttttt                                            <  2:895577/93‑1 (MQ=255)

                                                   cTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTa                                           <  1:5073303/93‑1 (MQ=255)

                                                   cTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTa                                           >  1:1218410/1‑93 (MQ=255)

                                                   cTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTa                                           <  2:7037162/93‑1 (MQ=255)

                                                    ttAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTAt                                          >  1:6634011/1‑93 (MQ=255)

                                                    ttAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTAt                                          <  2:4209298/93‑1 (MQ=255)

                                                    ttAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTAt                                          <  1:7159706/93‑1 (MQ=255)

                                                       aaCATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCgg                                       >  1:4032333/1‑93 (MQ=255)

                                                        aCATACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGt                                      >  1:786786/1‑93 (MQ=255)

                                                          aTACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTaa                                    >  2:44985/1‑93 (MQ=255)

                                                           tACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTaaa                                   >  2:5072256/1‑93 (MQ=255)

                                                           tACCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTaaa                                   <  1:1834206/93‑1 (MQ=255)

                                                            aCCGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAAt                                  <  2:1334455/93‑1 (MQ=255)

                                                             ccGCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATg                                 <  2:2561716/93‑1 (MQ=255)

                                                               gCCCCATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTa                               <  1:6318342/93‑1 (MQ=255)

                                                                 cccATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTAtt                             >  1:931418/1‑93 (MQ=255)

                                                                 cccATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTAtt                             <  1:3401924/93‑1 (MQ=255)

                                                                   cATAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTgc                           <  1:2558747/93‑1 (MQ=255)

                                                                     tAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTgcgc                         >  2:2325935/1‑93 (MQ=255)

                                                                     tAGGTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTgcgc                         >  2:1679869/1‑93 (MQ=255)

                                                                        gTTGATTCTTCGTTGTCTATGGCCCATCCCGGAAGGCGTTTTTTTTGTAATT‑TTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAt                     <  1:6459116/93‑1 (MQ=255)

                                                                        gTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTa                      >  1:742312/1‑93 (MQ=255)

                                                                        gTTGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTa                      <  1:1589044/93‑1 (MQ=255)

                                                                         ttGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAt                     >  1:6860387/1‑93 (MQ=255)

                                                                          tGATTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAtg                    >  1:2659063/1‑93 (MQ=255)

                                                                            aTTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAtgtg                  >  1:2920415/1‑93 (MQ=255)

                                                                            aTTCTTCGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAtgtg                  <  2:5269418/93‑1 (MQ=255)

                                                                             ttcttcGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTgg                 <  1:3956240/93‑1 (MQ=255)

                                                                              tcttcGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGa                <  2:682136/93‑1 (MQ=255)

                                                                              tcttcGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGa                <  1:3115521/93‑1 (MQ=255)

                                                                               cttcGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAg               <  2:7140339/93‑1 (MQ=255)

                                                                                ttcGTTGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGt              <  2:1585332/93‑1 (MQ=255)

                                                                                    ttGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAAc          >  2:5524770/1‑93 (MQ=255)

                                                                                    ttGTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAAc          <  1:7068249/93‑1 (MQ=255)

                                                                                     tGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACg         >  2:4852648/1‑93 (MQ=255)

                                                                                      gTCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGt        >  1:235096/1‑93 (MQ=255)

                                                                                       tCTATAGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTg       <  2:5603557/93‑1 (MQ=255)

                                                                                         tataGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAg     <  2:3774665/93‑1 (MQ=255)

                                                                                         tATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAg     >  1:1729324/1‑93 (MQ=255)

                                                                                            ggCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGc  <  2:2983462/93‑1 (MQ=255)

GAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGC  >  NC_000913/4522713‑4522897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`