Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,555,245 T→C Y205C (TAC→TGC)  yjiE ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,2450TC87.0% 757.5 / 90.4 301Y205C (TAC→TGC) yjiEputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base T (23/16);  new base C (133/129);  total (156/145)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 301 total aligned reads displayed.

TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA  >  NC_000913/4555155‑4555336
                                                                                          |                                                                                           
tttAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAg                                                                                           >  1:4459551/1‑93 (MQ=255)
  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGa                                                                                         <  2:365170/93‑1 (MQ=255)
  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGa                                                                                         <  1:6941591/93‑1 (MQ=255)
  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGa                                                                                         >  2:744245/1‑93 (MQ=255)
    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       >  1:4343284/1‑93 (MQ=255)
    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       <  1:3110676/93‑1 (MQ=255)
    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       >  2:2245579/1‑93 (MQ=255)
      aGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTg                                                                                     <  2:4667629/93‑1 (MQ=255)
       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTGACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGc                                                                                    <  2:7256472/93‑1 (MQ=255)
       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGCTGc                                                                                    >  2:5810785/1‑93 (MQ=255)
       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGc                                                                                    >  2:4816460/1‑93 (MQ=255)
         tcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCgg                                                                                  >  1:5218682/1‑93 (MQ=255)
         tcgctcATCGAAGAGACAAAAAAGGAGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCgg                                                                                  >  2:1464130/1‑93 (MQ=255)
             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGt                                                                              >  1:437686/1‑93 (MQ=255)
             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGt                                                                              >  2:1931747/1‑93 (MQ=255)
              cATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTa                                                                             >  2:2120202/1‑93 (MQ=255)
               aTCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTaa                                                                            <  1:7106949/93‑1 (MQ=255)
               aTCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTaa                                                                            >  1:5460531/1‑93 (MQ=255)
                tCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAt                                                                           >  1:7401733/1‑93 (MQ=255)
                 cGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAtt                                                                          <  1:1296034/93‑1 (MQ=255)
                  gAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTc                                                                         >  2:5382511/1‑93 (MQ=255)
                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGCAATTCa                                                                        <  2:6988238/93‑1 (MQ=255)
                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTa                                                                     >  1:1007982/1‑93 (MQ=255)
                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTa                                                                     >  1:6102986/1‑93 (MQ=255)
                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  1:4747940/93‑1 (MQ=255)
                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  1:1390385/93‑1 (MQ=255)
                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCACGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  2:6416312/93‑1 (MQ=255)
                        aCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAc                                                                   >  1:1158234/1‑93 (MQ=255)
                         cAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACg                                                                  <  2:5874922/93‑1 (MQ=255)
                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 >  2:5031233/1‑93 (MQ=255)
                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 >  1:5076658/1‑93 (MQ=255)
                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 <  1:3029312/93‑1 (MQ=255)
                           aaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGa                                                                >  2:4091835/1‑93 (MQ=255)
                           aaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGa                                                                <  2:5747770/93‑1 (MQ=255)
                            aaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaa                                                               >  2:3794502/1‑93 (MQ=255)
                            aaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaa                                                               <  2:3493737/93‑1 (MQ=255)
                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              <  2:6883746/93‑1 (MQ=255)
                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              <  1:3998783/93‑1 (MQ=255)
                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              >  1:1691749/1‑93 (MQ=255)
                               aGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgt                                                            >  2:5644430/1‑93 (MQ=255)
                               aGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgt                                                            >  1:1768307/1‑93 (MQ=255)
                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          >  1:4550120/1‑93 (MQ=255)
                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          <  1:6448004/93‑1 (MQ=255)
                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          >  2:2386303/1‑93 (MQ=255)
                                  tGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGtgtg                                                         >  2:3413861/1‑93 (MQ=255)
                                   gCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTgg                                                        <  2:5781467/93‑1 (MQ=255)
                                    cTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                       <  1:4944528/93‑1 (MQ=255)
                                    cTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                       <  1:1462100/93‑1 (MQ=255)
                                     tGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCt                                                      <  1:6003049/93‑1 (MQ=255)
                                        aaCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcg                                                   >  1:3254441/1‑93 (MQ=255)
                                        aaCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcg                                                   >  2:7515725/1‑93 (MQ=255)
                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                  >  1:985909/1‑93 (MQ=255)
                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                  >  2:6732500/1‑93 (MQ=255)
                                           ttAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCACCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGa                                                <  2:7098096/93‑1 (MQ=255)
                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                             >  1:3584319/1‑93 (MQ=255)
                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                             >  1:2645932/1‑93 (MQ=255)
                                                tCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTa                                           >  1:386444/1‑93 (MQ=255)
                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaa                                          <  2:1801756/93‑1 (MQ=255)
                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaa                                          >  1:813149/1‑93 (MQ=255)
                                                  aCTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaa                                         >  2:2459825/1‑93 (MQ=255)
                                                  aCTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaa                                         >  2:2767764/1‑93 (MQ=255)
                                                    tgtgGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaaaa                                       >  1:2199522/1‑93 (MQ=255)
                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                              <  1:4137833/93‑1 (MQ=255)
                                                              cAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTcc                             <  1:6335298/93‑1 (MQ=255)
                                                              cAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTcc                             <  2:4440112/93‑1 (MQ=255)
                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:2586653/1‑93 (MQ=255)
                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:5197522/1‑93 (MQ=255)
                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:7184730/1‑93 (MQ=255)
                                                                gggTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:37020/93‑1 (MQ=255)
                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:1051716/93‑1 (MQ=255)
                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:2457641/93‑1 (MQ=255)
                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  2:2975333/93‑1 (MQ=255)
                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:2853375/93‑1 (MQ=255)
                                                                   tGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGtt                        <  1:277690/93‑1 (MQ=255)
                                                                    gCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTGAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTc                       >  2:6275211/1‑93 (MQ=255)
                                                                    gCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTc                       >  1:483688/1‑93 (MQ=255)
                                                                      gATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGt                     >  2:7286825/1‑93 (MQ=255)
                                                                      gATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGt                     >  1:1860251/1‑93 (MQ=255)
                                                                       aTTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTc                    <  2:3692476/93‑1 (MQ=255)
                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  >  2:607194/1‑93 (MQ=255)
                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  1:2321252/93‑1 (MQ=255)
                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  2:1182792/93‑1 (MQ=255)
                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGAGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  2:1288555/93‑1 (MQ=255)
                                                                          aatcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACt                 <  2:6535303/93‑1 (MQ=255)
                                                                          aatcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGACTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACt                 <  2:7381284/93‑1 (MQ=255)
                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  1:670597/93‑1 (MQ=255)
                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  2:3136509/93‑1 (MQ=255)
                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  1:4670000/93‑1 (MQ=255)
                                                                             caatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGgc              <  2:428926/93‑1 (MQ=255)
                                                                               atcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGgcgc            >  2:5339461/1‑93 (MQ=255)
                                                                                tcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCa           <  1:5392613/93‑1 (MQ=255)
                                                                                  gCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGa         <  2:1621690/93‑1 (MQ=255)
                                                                                   ccccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAc        <  2:1758205/93‑1 (MQ=255)
                                                                                    cccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACa       >  2:492422/1‑93 (MQ=255)
                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      >  2:5723987/1‑93 (MQ=255)
                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      >  2:3282110/1‑93 (MQ=255)
                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      <  1:2073004/93‑1 (MQ=255)
                                                                                      cATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAgg     >  1:5546677/1‑93 (MQ=255)
                                                                                       aTGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAggg    <  2:6228866/93‑1 (MQ=255)
                                                                                         gTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGaa  <  2:3994415/93‑1 (MQ=255)
                                                                                          |                                                                                           
TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA  >  NC_000913/4555155‑4555336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑